Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979
Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80...
Ausführliche Beschreibung
Autor*in: |
Dong, Yuesheng [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2014transfer abstract |
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Umfang: |
13 |
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Übergeordnetes Werk: |
Enthalten in: SP-0433: Imaging biobanks: challenges and opportunities - Van der Lugt, A. ELSEVIER, 2017, Amsterdam [u.a.] |
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Übergeordnetes Werk: |
volume:35 ; year:2014 ; number:9 ; pages:1534-1546 ; extent:13 |
Links: |
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DOI / URN: |
10.1016/S1872-2067(14)60134-0 |
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Katalog-ID: |
ELV02805377X |
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520 | |a Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. | ||
520 | |a Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. | ||
650 | 7 | |a Geniposide-β-glucosidase |2 Elsevier | |
650 | 7 | |a Geniposide |2 Elsevier | |
650 | 7 | |a Purification |2 Elsevier | |
650 | 7 | |a Biotransformation |2 Elsevier | |
650 | 7 | |a Trichoderma harzianum |2 Elsevier | |
650 | 7 | |a Genipin |2 Elsevier | |
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700 | 1 | |a Hao, Aiyu |4 oth | |
700 | 1 | |a Qin, Ying |4 oth | |
700 | 1 | |a Wen, Zujia |4 oth | |
700 | 1 | |a Xiu, Zhilong |4 oth | |
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10.1016/S1872-2067(14)60134-0 doi GBVA2014009000015.pica (DE-627)ELV02805377X (ELSEVIER)S1872-2067(14)60134-0 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 570 540 VZ Dong, Yuesheng verfasserin aut Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979 2014transfer abstract 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. Geniposide-β-glucosidase Elsevier Geniposide Elsevier Purification Elsevier Biotransformation Elsevier Trichoderma harzianum Elsevier Genipin Elsevier Liu, Leping oth Bao, Yongming oth Hao, Aiyu oth Qin, Ying oth Wen, Zujia oth Xiu, Zhilong oth Enthalten in Elsevier Van der Lugt, A. ELSEVIER SP-0433: Imaging biobanks: challenges and opportunities 2017 Amsterdam [u.a.] (DE-627)ELV01487606X volume:35 year:2014 number:9 pages:1534-1546 extent:13 https://doi.org/10.1016/S1872-2067(14)60134-0 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 AR 35 2014 9 1534-1546 13 045F 540 |
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10.1016/S1872-2067(14)60134-0 doi GBVA2014009000015.pica (DE-627)ELV02805377X (ELSEVIER)S1872-2067(14)60134-0 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 570 540 VZ Dong, Yuesheng verfasserin aut Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979 2014transfer abstract 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. Geniposide-β-glucosidase Elsevier Geniposide Elsevier Purification Elsevier Biotransformation Elsevier Trichoderma harzianum Elsevier Genipin Elsevier Liu, Leping oth Bao, Yongming oth Hao, Aiyu oth Qin, Ying oth Wen, Zujia oth Xiu, Zhilong oth Enthalten in Elsevier Van der Lugt, A. ELSEVIER SP-0433: Imaging biobanks: challenges and opportunities 2017 Amsterdam [u.a.] (DE-627)ELV01487606X volume:35 year:2014 number:9 pages:1534-1546 extent:13 https://doi.org/10.1016/S1872-2067(14)60134-0 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 AR 35 2014 9 1534-1546 13 045F 540 |
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10.1016/S1872-2067(14)60134-0 doi GBVA2014009000015.pica (DE-627)ELV02805377X (ELSEVIER)S1872-2067(14)60134-0 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 570 540 VZ Dong, Yuesheng verfasserin aut Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979 2014transfer abstract 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. Geniposide-β-glucosidase Elsevier Geniposide Elsevier Purification Elsevier Biotransformation Elsevier Trichoderma harzianum Elsevier Genipin Elsevier Liu, Leping oth Bao, Yongming oth Hao, Aiyu oth Qin, Ying oth Wen, Zujia oth Xiu, Zhilong oth Enthalten in Elsevier Van der Lugt, A. ELSEVIER SP-0433: Imaging biobanks: challenges and opportunities 2017 Amsterdam [u.a.] (DE-627)ELV01487606X volume:35 year:2014 number:9 pages:1534-1546 extent:13 https://doi.org/10.1016/S1872-2067(14)60134-0 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 AR 35 2014 9 1534-1546 13 045F 540 |
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10.1016/S1872-2067(14)60134-0 doi GBVA2014009000015.pica (DE-627)ELV02805377X (ELSEVIER)S1872-2067(14)60134-0 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 570 540 VZ Dong, Yuesheng verfasserin aut Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979 2014transfer abstract 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. Geniposide-β-glucosidase Elsevier Geniposide Elsevier Purification Elsevier Biotransformation Elsevier Trichoderma harzianum Elsevier Genipin Elsevier Liu, Leping oth Bao, Yongming oth Hao, Aiyu oth Qin, Ying oth Wen, Zujia oth Xiu, Zhilong oth Enthalten in Elsevier Van der Lugt, A. ELSEVIER SP-0433: Imaging biobanks: challenges and opportunities 2017 Amsterdam [u.a.] (DE-627)ELV01487606X volume:35 year:2014 number:9 pages:1534-1546 extent:13 https://doi.org/10.1016/S1872-2067(14)60134-0 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 AR 35 2014 9 1534-1546 13 045F 540 |
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10.1016/S1872-2067(14)60134-0 doi GBVA2014009000015.pica (DE-627)ELV02805377X (ELSEVIER)S1872-2067(14)60134-0 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 570 540 VZ Dong, Yuesheng verfasserin aut Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979 2014transfer abstract 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. Geniposide-β-glucosidase Elsevier Geniposide Elsevier Purification Elsevier Biotransformation Elsevier Trichoderma harzianum Elsevier Genipin Elsevier Liu, Leping oth Bao, Yongming oth Hao, Aiyu oth Qin, Ying oth Wen, Zujia oth Xiu, Zhilong oth Enthalten in Elsevier Van der Lugt, A. ELSEVIER SP-0433: Imaging biobanks: challenges and opportunities 2017 Amsterdam [u.a.] (DE-627)ELV01487606X volume:35 year:2014 number:9 pages:1534-1546 extent:13 https://doi.org/10.1016/S1872-2067(14)60134-0 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 AR 35 2014 9 1534-1546 13 045F 540 |
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biotransformation of geniposide in gardenia jasminoides to genipin by trichoderma harzianum cgmcc 2979 |
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Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979 |
abstract |
Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. |
abstractGer |
Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. |
abstract_unstemmed |
Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0–5.0. The K m and V max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin. |
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Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979 |
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