Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives
A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic comp...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Noda, Shuhei [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016transfer abstract |
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| Schlagwörter: |
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| Umfang: |
11 |
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| Übergeordnetes Werk: |
Enthalten in: Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery - Gao, Mengyao ELSEVIER, 2014transfer abstract, Orlando, Fla |
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| Übergeordnetes Werk: |
volume:33 ; year:2016 ; pages:119-129 ; extent:11 |
| Links: |
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| DOI / URN: |
10.1016/j.ymben.2015.11.007 |
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| 245 | 1 | 0 | |a Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives |
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| 520 | |a A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. | ||
| 520 | |a A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. | ||
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10.1016/j.ymben.2015.11.007 doi GBV00000000000154A.pica (DE-627)ELV029959136 (ELSEVIER)S1096-7176(15)00156-1 DE-627 ger DE-627 rakwb eng 610 610 DE-600 540 VZ 610 VZ 44.00 bkl Noda, Shuhei verfasserin aut Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives 2016transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. E4P Elsevier Csr Elsevier 3HBA Elsevier PTS Elsevier CDO Elsevier TPL Elsevier PHBA Elsevier MA Elsevier SMO Elsevier PABA Elsevier ICS Elsevier 2ABA Elsevier PEP Elsevier DAHP Elsevier PCR Elsevier Shirai, Tomokazu oth Oyama, Sachiko oth Kondo, Akihiko oth Enthalten in Academic Press Gao, Mengyao ELSEVIER Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery 2014transfer abstract Orlando, Fla (DE-627)ELV012555568 volume:33 year:2016 pages:119-129 extent:11 https://doi.org/10.1016/j.ymben.2015.11.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 44.00 Medizin: Allgemeines VZ AR 33 2016 119-129 11 045F 610 |
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10.1016/j.ymben.2015.11.007 doi GBV00000000000154A.pica (DE-627)ELV029959136 (ELSEVIER)S1096-7176(15)00156-1 DE-627 ger DE-627 rakwb eng 610 610 DE-600 540 VZ 610 VZ 44.00 bkl Noda, Shuhei verfasserin aut Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives 2016transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. E4P Elsevier Csr Elsevier 3HBA Elsevier PTS Elsevier CDO Elsevier TPL Elsevier PHBA Elsevier MA Elsevier SMO Elsevier PABA Elsevier ICS Elsevier 2ABA Elsevier PEP Elsevier DAHP Elsevier PCR Elsevier Shirai, Tomokazu oth Oyama, Sachiko oth Kondo, Akihiko oth Enthalten in Academic Press Gao, Mengyao ELSEVIER Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery 2014transfer abstract Orlando, Fla (DE-627)ELV012555568 volume:33 year:2016 pages:119-129 extent:11 https://doi.org/10.1016/j.ymben.2015.11.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 44.00 Medizin: Allgemeines VZ AR 33 2016 119-129 11 045F 610 |
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10.1016/j.ymben.2015.11.007 doi GBV00000000000154A.pica (DE-627)ELV029959136 (ELSEVIER)S1096-7176(15)00156-1 DE-627 ger DE-627 rakwb eng 610 610 DE-600 540 VZ 610 VZ 44.00 bkl Noda, Shuhei verfasserin aut Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives 2016transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. E4P Elsevier Csr Elsevier 3HBA Elsevier PTS Elsevier CDO Elsevier TPL Elsevier PHBA Elsevier MA Elsevier SMO Elsevier PABA Elsevier ICS Elsevier 2ABA Elsevier PEP Elsevier DAHP Elsevier PCR Elsevier Shirai, Tomokazu oth Oyama, Sachiko oth Kondo, Akihiko oth Enthalten in Academic Press Gao, Mengyao ELSEVIER Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery 2014transfer abstract Orlando, Fla (DE-627)ELV012555568 volume:33 year:2016 pages:119-129 extent:11 https://doi.org/10.1016/j.ymben.2015.11.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 44.00 Medizin: Allgemeines VZ AR 33 2016 119-129 11 045F 610 |
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10.1016/j.ymben.2015.11.007 doi GBV00000000000154A.pica (DE-627)ELV029959136 (ELSEVIER)S1096-7176(15)00156-1 DE-627 ger DE-627 rakwb eng 610 610 DE-600 540 VZ 610 VZ 44.00 bkl Noda, Shuhei verfasserin aut Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives 2016transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. E4P Elsevier Csr Elsevier 3HBA Elsevier PTS Elsevier CDO Elsevier TPL Elsevier PHBA Elsevier MA Elsevier SMO Elsevier PABA Elsevier ICS Elsevier 2ABA Elsevier PEP Elsevier DAHP Elsevier PCR Elsevier Shirai, Tomokazu oth Oyama, Sachiko oth Kondo, Akihiko oth Enthalten in Academic Press Gao, Mengyao ELSEVIER Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery 2014transfer abstract Orlando, Fla (DE-627)ELV012555568 volume:33 year:2016 pages:119-129 extent:11 https://doi.org/10.1016/j.ymben.2015.11.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 44.00 Medizin: Allgemeines VZ AR 33 2016 119-129 11 045F 610 |
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10.1016/j.ymben.2015.11.007 doi GBV00000000000154A.pica (DE-627)ELV029959136 (ELSEVIER)S1096-7176(15)00156-1 DE-627 ger DE-627 rakwb eng 610 610 DE-600 540 VZ 610 VZ 44.00 bkl Noda, Shuhei verfasserin aut Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives 2016transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. E4P Elsevier Csr Elsevier 3HBA Elsevier PTS Elsevier CDO Elsevier TPL Elsevier PHBA Elsevier MA Elsevier SMO Elsevier PABA Elsevier ICS Elsevier 2ABA Elsevier PEP Elsevier DAHP Elsevier PCR Elsevier Shirai, Tomokazu oth Oyama, Sachiko oth Kondo, Akihiko oth Enthalten in Academic Press Gao, Mengyao ELSEVIER Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery 2014transfer abstract Orlando, Fla (DE-627)ELV012555568 volume:33 year:2016 pages:119-129 extent:11 https://doi.org/10.1016/j.ymben.2015.11.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 44.00 Medizin: Allgemeines VZ AR 33 2016 119-129 11 045F 610 |
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Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives |
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A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. |
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A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. |
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A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture. |
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Each of these industrially important chemicals was successfully produced to levels of 1–3g/L in test tube-scale culture.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a high-demand aromatic chemical. The titer of salicylate reached 11.5g/L in batch culture after 48h cultivation in a 2-liter jar fermentor, and the yield from glucose as the sole carbon source exceeded 40% (mol/mol). In this test case, we found that pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruvate. In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives. 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