Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan
• A syber green based quantitative real-time PCR (qPCR) assay was optimized and then detection of all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established.A syber green based quantitative real-time PCR (qPCR) assay was optimized for...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Shafiq, Muhammad [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Schlagwörter: |
Cotton leaf curl Multan betasatellite (CLCuMuB) Polymerase chain reaction (PCR) Cotton leaf curl disease (CLCuD) |
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| Umfang: |
7 |
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| Übergeordnetes Werk: |
Enthalten in: Perpendicular magnetic anisotropy in Ta/CoFeB/MgO systems synthesized on treated SiN/SiO2 substrates for magnetic memories - 2013transfer abstract, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:248 ; year:2017 ; pages:54-60 ; extent:7 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.jviromet.2017.05.012 |
|---|
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ELV030310776 |
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| 520 | |a • A syber green based quantitative real-time PCR (qPCR) assay was optimized and then detection of all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established.A syber green based quantitative real-time PCR (qPCR) assay was optimized for quantification of CLCuD causing viruses. • This viral/satellite quantification was used to investigate the relationship between disease severity scales and virus/satellite titre. • No direct correlation was found with viral load and symptom severity. | ||
| 650 | 7 | |a Cotton leaf curl Multan betasatellite (CLCuMuB) |2 Elsevier | |
| 650 | 7 | |a Polymerase chain reaction (PCR) |2 Elsevier | |
| 650 | 7 | |a Cotton leaf curl disease (CLCuD) |2 Elsevier | |
| 650 | 7 | |a Cotton leaf curl Khokhran virus − Burewala (CLCuKoV-Bu) |2 Elsevier | |
| 650 | 7 | |a Quantitative real-time PCR (qPCR) |2 Elsevier | |
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10.1016/j.jviromet.2017.05.012 doi GBV00000000000255A.pica (DE-627)ELV030310776 (ELSEVIER)S0166-0934(16)30523-7 DE-627 ger DE-627 rakwb eng 610 610 DE-600 070 VZ 660 VZ 333.7 610 VZ 43.12 bkl 43.13 bkl 44.13 bkl Shafiq, Muhammad verfasserin aut Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan 2017 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier • A syber green based quantitative real-time PCR (qPCR) assay was optimized and then detection of all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established.A syber green based quantitative real-time PCR (qPCR) assay was optimized for quantification of CLCuD causing viruses. • This viral/satellite quantification was used to investigate the relationship between disease severity scales and virus/satellite titre. • No direct correlation was found with viral load and symptom severity. Cotton leaf curl Multan betasatellite (CLCuMuB) Elsevier Polymerase chain reaction (PCR) Elsevier Cotton leaf curl disease (CLCuD) Elsevier Cotton leaf curl Khokhran virus − Burewala (CLCuKoV-Bu) Elsevier Quantitative real-time PCR (qPCR) Elsevier Iqbal, Zafar oth Ali, Irfan oth Abbas, Qamar oth Mansoor, Shahid oth Briddon, Rob. W. oth Amin, Imran oth Enthalten in Elsevier Science Perpendicular magnetic anisotropy in Ta/CoFeB/MgO systems synthesized on treated SiN/SiO2 substrates for magnetic memories 2013transfer abstract Amsterdam [u.a.] (DE-627)ELV011297778 volume:248 year:2017 pages:54-60 extent:7 https://doi.org/10.1016/j.jviromet.2017.05.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-GGO GBV_ILN_70 43.12 Umweltchemie VZ 43.13 Umwelttoxikologie VZ 44.13 Medizinische Ökologie VZ AR 248 2017 54-60 7 045F 610 |
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10.1016/j.jviromet.2017.05.012 doi GBV00000000000255A.pica (DE-627)ELV030310776 (ELSEVIER)S0166-0934(16)30523-7 DE-627 ger DE-627 rakwb eng 610 610 DE-600 070 VZ 660 VZ 333.7 610 VZ 43.12 bkl 43.13 bkl 44.13 bkl Shafiq, Muhammad verfasserin aut Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan 2017 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier • A syber green based quantitative real-time PCR (qPCR) assay was optimized and then detection of all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established.A syber green based quantitative real-time PCR (qPCR) assay was optimized for quantification of CLCuD causing viruses. • This viral/satellite quantification was used to investigate the relationship between disease severity scales and virus/satellite titre. • No direct correlation was found with viral load and symptom severity. Cotton leaf curl Multan betasatellite (CLCuMuB) Elsevier Polymerase chain reaction (PCR) Elsevier Cotton leaf curl disease (CLCuD) Elsevier Cotton leaf curl Khokhran virus − Burewala (CLCuKoV-Bu) Elsevier Quantitative real-time PCR (qPCR) Elsevier Iqbal, Zafar oth Ali, Irfan oth Abbas, Qamar oth Mansoor, Shahid oth Briddon, Rob. W. oth Amin, Imran oth Enthalten in Elsevier Science Perpendicular magnetic anisotropy in Ta/CoFeB/MgO systems synthesized on treated SiN/SiO2 substrates for magnetic memories 2013transfer abstract Amsterdam [u.a.] (DE-627)ELV011297778 volume:248 year:2017 pages:54-60 extent:7 https://doi.org/10.1016/j.jviromet.2017.05.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-GGO GBV_ILN_70 43.12 Umweltchemie VZ 43.13 Umwelttoxikologie VZ 44.13 Medizinische Ökologie VZ AR 248 2017 54-60 7 045F 610 |
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10.1016/j.jviromet.2017.05.012 doi GBV00000000000255A.pica (DE-627)ELV030310776 (ELSEVIER)S0166-0934(16)30523-7 DE-627 ger DE-627 rakwb eng 610 610 DE-600 070 VZ 660 VZ 333.7 610 VZ 43.12 bkl 43.13 bkl 44.13 bkl Shafiq, Muhammad verfasserin aut Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan 2017 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier • A syber green based quantitative real-time PCR (qPCR) assay was optimized and then detection of all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established.A syber green based quantitative real-time PCR (qPCR) assay was optimized for quantification of CLCuD causing viruses. • This viral/satellite quantification was used to investigate the relationship between disease severity scales and virus/satellite titre. • No direct correlation was found with viral load and symptom severity. Cotton leaf curl Multan betasatellite (CLCuMuB) Elsevier Polymerase chain reaction (PCR) Elsevier Cotton leaf curl disease (CLCuD) Elsevier Cotton leaf curl Khokhran virus − Burewala (CLCuKoV-Bu) Elsevier Quantitative real-time PCR (qPCR) Elsevier Iqbal, Zafar oth Ali, Irfan oth Abbas, Qamar oth Mansoor, Shahid oth Briddon, Rob. W. oth Amin, Imran oth Enthalten in Elsevier Science Perpendicular magnetic anisotropy in Ta/CoFeB/MgO systems synthesized on treated SiN/SiO2 substrates for magnetic memories 2013transfer abstract Amsterdam [u.a.] (DE-627)ELV011297778 volume:248 year:2017 pages:54-60 extent:7 https://doi.org/10.1016/j.jviromet.2017.05.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-GGO GBV_ILN_70 43.12 Umweltchemie VZ 43.13 Umwelttoxikologie VZ 44.13 Medizinische Ökologie VZ AR 248 2017 54-60 7 045F 610 |
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Cotton leaf curl Multan betasatellite (CLCuMuB) Polymerase chain reaction (PCR) Cotton leaf curl disease (CLCuD) Cotton leaf curl Khokhran virus − Burewala (CLCuKoV-Bu) Quantitative real-time PCR (qPCR) |
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Perpendicular magnetic anisotropy in Ta/CoFeB/MgO systems synthesized on treated SiN/SiO2 substrates for magnetic memories |
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Perpendicular magnetic anisotropy in Ta/CoFeB/MgO systems synthesized on treated SiN/SiO2 substrates for magnetic memories |
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Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan |
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Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan |
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Shafiq, Muhammad |
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Perpendicular magnetic anisotropy in Ta/CoFeB/MgO systems synthesized on treated SiN/SiO2 substrates for magnetic memories |
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real-time quantitative pcr assay for the quantification of virus and satellites causing leaf curl disease in cotton in pakistan |
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Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan |
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• A syber green based quantitative real-time PCR (qPCR) assay was optimized and then detection of all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established.A syber green based quantitative real-time PCR (qPCR) assay was optimized for quantification of CLCuD causing viruses. • This viral/satellite quantification was used to investigate the relationship between disease severity scales and virus/satellite titre. • No direct correlation was found with viral load and symptom severity. |
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• A syber green based quantitative real-time PCR (qPCR) assay was optimized and then detection of all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established.A syber green based quantitative real-time PCR (qPCR) assay was optimized for quantification of CLCuD causing viruses. • This viral/satellite quantification was used to investigate the relationship between disease severity scales and virus/satellite titre. • No direct correlation was found with viral load and symptom severity. |
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• A syber green based quantitative real-time PCR (qPCR) assay was optimized and then detection of all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established.A syber green based quantitative real-time PCR (qPCR) assay was optimized for quantification of CLCuD causing viruses. • This viral/satellite quantification was used to investigate the relationship between disease severity scales and virus/satellite titre. • No direct correlation was found with viral load and symptom severity. |
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Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan |
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Iqbal, Zafar Ali, Irfan Abbas, Qamar Mansoor, Shahid Briddon, Rob. W. Amin, Imran |
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