Enrichment of cells with TALEN-induced mutations using surrogate reporters
Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always h...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kim, Young-Hoon [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014transfer abstract |
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| Schlagwörter: |
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| Umfang: |
10 |
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| Übergeordnetes Werk: |
Enthalten in: Calculating melting temperatures and pressures of peridotite protoliths: Implications for the origin of cratonic mantle - Lee, Cin-Ty A. ELSEVIER, 2014transfer abstract, a companion to methods in enzymology, Orlando, Fla |
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| Übergeordnetes Werk: |
volume:69 ; year:2014 ; number:1 ; day:15 ; month:08 ; pages:108-117 ; extent:10 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.ymeth.2014.04.012 |
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ELV034123377 |
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| 520 | |a Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. | ||
| 520 | |a Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. | ||
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10.1016/j.ymeth.2014.04.012 doi GBVA2014017000023.pica (DE-627)ELV034123377 (ELSEVIER)S1046-2023(14)00165-0 DE-627 ger DE-627 rakwb eng 540 540 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl Kim, Young-Hoon verfasserin aut Enrichment of cells with TALEN-induced mutations using surrogate reporters 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. Transcription activator-like effector nucleases Elsevier Enrichment Elsevier Flow cytometry Elsevier Magnetic separation Elsevier Hygromycin B Elsevier Mutant cells Elsevier Ramakrishna, Suresh oth Kim, Hyongbum oth Kim, Jin-Soo oth Enthalten in Academic Press Lee, Cin-Ty A. ELSEVIER Calculating melting temperatures and pressures of peridotite protoliths: Implications for the origin of cratonic mantle 2014transfer abstract a companion to methods in enzymology Orlando, Fla (DE-627)ELV023049820 volume:69 year:2014 number:1 day:15 month:08 pages:108-117 extent:10 https://doi.org/10.1016/j.ymeth.2014.04.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 42.90 Ökologie: Allgemeines VZ 42.11 Biomathematik Biokybernetik VZ AR 69 2014 1 15 0815 108-117 10 045F 540 |
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10.1016/j.ymeth.2014.04.012 doi GBVA2014017000023.pica (DE-627)ELV034123377 (ELSEVIER)S1046-2023(14)00165-0 DE-627 ger DE-627 rakwb eng 540 540 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl Kim, Young-Hoon verfasserin aut Enrichment of cells with TALEN-induced mutations using surrogate reporters 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. Transcription activator-like effector nucleases Elsevier Enrichment Elsevier Flow cytometry Elsevier Magnetic separation Elsevier Hygromycin B Elsevier Mutant cells Elsevier Ramakrishna, Suresh oth Kim, Hyongbum oth Kim, Jin-Soo oth Enthalten in Academic Press Lee, Cin-Ty A. ELSEVIER Calculating melting temperatures and pressures of peridotite protoliths: Implications for the origin of cratonic mantle 2014transfer abstract a companion to methods in enzymology Orlando, Fla (DE-627)ELV023049820 volume:69 year:2014 number:1 day:15 month:08 pages:108-117 extent:10 https://doi.org/10.1016/j.ymeth.2014.04.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 42.90 Ökologie: Allgemeines VZ 42.11 Biomathematik Biokybernetik VZ AR 69 2014 1 15 0815 108-117 10 045F 540 |
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10.1016/j.ymeth.2014.04.012 doi GBVA2014017000023.pica (DE-627)ELV034123377 (ELSEVIER)S1046-2023(14)00165-0 DE-627 ger DE-627 rakwb eng 540 540 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl Kim, Young-Hoon verfasserin aut Enrichment of cells with TALEN-induced mutations using surrogate reporters 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. Transcription activator-like effector nucleases Elsevier Enrichment Elsevier Flow cytometry Elsevier Magnetic separation Elsevier Hygromycin B Elsevier Mutant cells Elsevier Ramakrishna, Suresh oth Kim, Hyongbum oth Kim, Jin-Soo oth Enthalten in Academic Press Lee, Cin-Ty A. ELSEVIER Calculating melting temperatures and pressures of peridotite protoliths: Implications for the origin of cratonic mantle 2014transfer abstract a companion to methods in enzymology Orlando, Fla (DE-627)ELV023049820 volume:69 year:2014 number:1 day:15 month:08 pages:108-117 extent:10 https://doi.org/10.1016/j.ymeth.2014.04.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 42.90 Ökologie: Allgemeines VZ 42.11 Biomathematik Biokybernetik VZ AR 69 2014 1 15 0815 108-117 10 045F 540 |
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10.1016/j.ymeth.2014.04.012 doi GBVA2014017000023.pica (DE-627)ELV034123377 (ELSEVIER)S1046-2023(14)00165-0 DE-627 ger DE-627 rakwb eng 540 540 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl Kim, Young-Hoon verfasserin aut Enrichment of cells with TALEN-induced mutations using surrogate reporters 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. Transcription activator-like effector nucleases Elsevier Enrichment Elsevier Flow cytometry Elsevier Magnetic separation Elsevier Hygromycin B Elsevier Mutant cells Elsevier Ramakrishna, Suresh oth Kim, Hyongbum oth Kim, Jin-Soo oth Enthalten in Academic Press Lee, Cin-Ty A. ELSEVIER Calculating melting temperatures and pressures of peridotite protoliths: Implications for the origin of cratonic mantle 2014transfer abstract a companion to methods in enzymology Orlando, Fla (DE-627)ELV023049820 volume:69 year:2014 number:1 day:15 month:08 pages:108-117 extent:10 https://doi.org/10.1016/j.ymeth.2014.04.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 42.90 Ökologie: Allgemeines VZ 42.11 Biomathematik Biokybernetik VZ AR 69 2014 1 15 0815 108-117 10 045F 540 |
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10.1016/j.ymeth.2014.04.012 doi GBVA2014017000023.pica (DE-627)ELV034123377 (ELSEVIER)S1046-2023(14)00165-0 DE-627 ger DE-627 rakwb eng 540 540 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl Kim, Young-Hoon verfasserin aut Enrichment of cells with TALEN-induced mutations using surrogate reporters 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. Transcription activator-like effector nucleases Elsevier Enrichment Elsevier Flow cytometry Elsevier Magnetic separation Elsevier Hygromycin B Elsevier Mutant cells Elsevier Ramakrishna, Suresh oth Kim, Hyongbum oth Kim, Jin-Soo oth Enthalten in Academic Press Lee, Cin-Ty A. ELSEVIER Calculating melting temperatures and pressures of peridotite protoliths: Implications for the origin of cratonic mantle 2014transfer abstract a companion to methods in enzymology Orlando, Fla (DE-627)ELV023049820 volume:69 year:2014 number:1 day:15 month:08 pages:108-117 extent:10 https://doi.org/10.1016/j.ymeth.2014.04.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 42.90 Ökologie: Allgemeines VZ 42.11 Biomathematik Biokybernetik VZ AR 69 2014 1 15 0815 108-117 10 045F 540 |
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Enthalten in Calculating melting temperatures and pressures of peridotite protoliths: Implications for the origin of cratonic mantle Orlando, Fla volume:69 year:2014 number:1 day:15 month:08 pages:108-117 extent:10 |
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Enthalten in Calculating melting temperatures and pressures of peridotite protoliths: Implications for the origin of cratonic mantle Orlando, Fla volume:69 year:2014 number:1 day:15 month:08 pages:108-117 extent:10 |
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Calculating melting temperatures and pressures of peridotite protoliths: Implications for the origin of cratonic mantle |
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Kim, Young-Hoon @@aut@@ Ramakrishna, Suresh @@oth@@ Kim, Hyongbum @@oth@@ Kim, Jin-Soo @@oth@@ |
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Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. |
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Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. |
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Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. |
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