Efficient isolation of CD8α positive T cells from postnatal mice using a combined MACS approach
Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Hübner, Anastasia [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014transfer abstract |
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| Umfang: |
7 |
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| Übergeordnetes Werk: |
Enthalten in: 969: Cesarean prevalence rates overtime by maternal characteristics - Thompson, Jennifer A. ELSEVIER, 2018, JIM, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:406 ; year:2014 ; pages:110-116 ; extent:7 |
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| DOI / URN: |
10.1016/j.jim.2014.02.006 |
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| 520 | |a Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. | ||
| 520 | |a Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. | ||
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10.1016/j.jim.2014.02.006 doi GBVA2014022000025.pica (DE-627)ELV034266569 (ELSEVIER)S0022-1759(14)00052-0 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 44.92 bkl Hübner, Anastasia verfasserin aut Efficient isolation of CD8α positive T cells from postnatal mice using a combined MACS approach 2014transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. Derkow, Katja oth Bräuer, Anja U. oth Enthalten in Elsevier Science Thompson, Jennifer A. ELSEVIER 969: Cesarean prevalence rates overtime by maternal characteristics 2018 JIM Amsterdam [u.a.] (DE-627)ELV001297813 volume:406 year:2014 pages:110-116 extent:7 https://doi.org/10.1016/j.jim.2014.02.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.92 Gynäkologie VZ AR 406 2014 110-116 7 045F 610 |
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10.1016/j.jim.2014.02.006 doi GBVA2014022000025.pica (DE-627)ELV034266569 (ELSEVIER)S0022-1759(14)00052-0 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 44.92 bkl Hübner, Anastasia verfasserin aut Efficient isolation of CD8α positive T cells from postnatal mice using a combined MACS approach 2014transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. Derkow, Katja oth Bräuer, Anja U. oth Enthalten in Elsevier Science Thompson, Jennifer A. ELSEVIER 969: Cesarean prevalence rates overtime by maternal characteristics 2018 JIM Amsterdam [u.a.] (DE-627)ELV001297813 volume:406 year:2014 pages:110-116 extent:7 https://doi.org/10.1016/j.jim.2014.02.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.92 Gynäkologie VZ AR 406 2014 110-116 7 045F 610 |
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10.1016/j.jim.2014.02.006 doi GBVA2014022000025.pica (DE-627)ELV034266569 (ELSEVIER)S0022-1759(14)00052-0 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 44.92 bkl Hübner, Anastasia verfasserin aut Efficient isolation of CD8α positive T cells from postnatal mice using a combined MACS approach 2014transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. Derkow, Katja oth Bräuer, Anja U. oth Enthalten in Elsevier Science Thompson, Jennifer A. ELSEVIER 969: Cesarean prevalence rates overtime by maternal characteristics 2018 JIM Amsterdam [u.a.] (DE-627)ELV001297813 volume:406 year:2014 pages:110-116 extent:7 https://doi.org/10.1016/j.jim.2014.02.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.92 Gynäkologie VZ AR 406 2014 110-116 7 045F 610 |
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10.1016/j.jim.2014.02.006 doi GBVA2014022000025.pica (DE-627)ELV034266569 (ELSEVIER)S0022-1759(14)00052-0 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 44.92 bkl Hübner, Anastasia verfasserin aut Efficient isolation of CD8α positive T cells from postnatal mice using a combined MACS approach 2014transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. Derkow, Katja oth Bräuer, Anja U. oth Enthalten in Elsevier Science Thompson, Jennifer A. ELSEVIER 969: Cesarean prevalence rates overtime by maternal characteristics 2018 JIM Amsterdam [u.a.] (DE-627)ELV001297813 volume:406 year:2014 pages:110-116 extent:7 https://doi.org/10.1016/j.jim.2014.02.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.92 Gynäkologie VZ AR 406 2014 110-116 7 045F 610 |
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10.1016/j.jim.2014.02.006 doi GBVA2014022000025.pica (DE-627)ELV034266569 (ELSEVIER)S0022-1759(14)00052-0 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 44.92 bkl Hübner, Anastasia verfasserin aut Efficient isolation of CD8α positive T cells from postnatal mice using a combined MACS approach 2014transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. Derkow, Katja oth Bräuer, Anja U. oth Enthalten in Elsevier Science Thompson, Jennifer A. ELSEVIER 969: Cesarean prevalence rates overtime by maternal characteristics 2018 JIM Amsterdam [u.a.] (DE-627)ELV001297813 volume:406 year:2014 pages:110-116 extent:7 https://doi.org/10.1016/j.jim.2014.02.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.92 Gynäkologie VZ AR 406 2014 110-116 7 045F 610 |
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efficient isolation of cd8α positive t cells from postnatal mice using a combined macs approach |
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Efficient isolation of CD8α positive T cells from postnatal mice using a combined MACS approach |
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Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. |
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Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. |
| abstract_unstemmed |
Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications. |
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| title_short |
Efficient isolation of CD8α positive T cells from postnatal mice using a combined MACS approach |
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https://doi.org/10.1016/j.jim.2014.02.006 |
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| author2 |
Derkow, Katja Bräuer, Anja U. |
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Derkow, Katja Bräuer, Anja U. |
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| doi_str |
10.1016/j.jim.2014.02.006 |
| up_date |
2024-07-06T20:41:06.867Z |
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