Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2

Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grap...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Navarro, Sara [verfasserIn] Vazquez-Hernandez, María Rosales, Raquel Sanchez-Ballesta, María Teresa Merodio, Carmen Escribano, María Isabel

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015transfer abstract

Schlagwörter:	Trehalose Dehydrin gene expression Cold storage Dehydrin isoforms Vitis vinifera

Umfang:	11

Übergeordnetes Werk:	Enthalten in: Continuity of ambulatory care and health outcomes in adult patients with type 2 diabetes in Korea - 2013, biochemistry, physiology, molecular biology and biotechnology of plants, München
Übergeordnetes Werk:	volume:179 ; year:2015 ; day:1 ; month:05 ; pages:1-11 ; extent:11

Links:	Volltext

DOI / URN:	10.1016/j.jplph.2015.02.007

Katalog-ID:	ELV034454179

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245	1	0	\|a Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2
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520			\|a Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage.
520			\|a Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage.
650		7	\|a Trehalose \|2 Elsevier
650		7	\|a Dehydrin gene expression \|2 Elsevier
650		7	\|a Cold storage \|2 Elsevier
650		7	\|a Dehydrin isoforms \|2 Elsevier
650		7	\|a Vitis vinifera \|2 Elsevier
700	1		\|a Vazquez-Hernandez, María \|4 oth
700	1		\|a Rosales, Raquel \|4 oth
700	1		\|a Sanchez-Ballesta, María Teresa \|4 oth
700	1		\|a Merodio, Carmen \|4 oth
700	1		\|a Escribano, María Isabel \|4 oth
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author_variant	s n sn
matchkey_str	navarrosaravazquezhernandezmararosalesra:2015----:ifrnileuainfeyrnxrsinnteaoeeesnadnlalgae
hierarchy_sort_str	2015transfer abstract
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allfields	10.1016/j.jplph.2015.02.007 doi GBVA2015006000024.pica (DE-627)ELV034454179 (ELSEVIER)S0176-1617(15)00047-4 DE-627 ger DE-627 rakwb eng 580 580 DE-600 610 VZ 630 640 VZ 48.00 bkl Navarro, Sara verfasserin aut Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2 2015transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage. Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage. Trehalose Elsevier Dehydrin gene expression Elsevier Cold storage Elsevier Dehydrin isoforms Elsevier Vitis vinifera Elsevier Vazquez-Hernandez, María oth Rosales, Raquel oth Sanchez-Ballesta, María Teresa oth Merodio, Carmen oth Escribano, María Isabel oth Enthalten in Elsevier Continuity of ambulatory care and health outcomes in adult patients with type 2 diabetes in Korea 2013 biochemistry, physiology, molecular biology and biotechnology of plants München (DE-627)ELV021744629 volume:179 year:2015 day:1 month:05 pages:1-11 extent:11 https://doi.org/10.1016/j.jplph.2015.02.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-FOR GBV_ILN_22 GBV_ILN_31 GBV_ILN_70 GBV_ILN_78 GBV_ILN_90 GBV_ILN_120 GBV_ILN_121 GBV_ILN_130 GBV_ILN_131 GBV_ILN_181 GBV_ILN_709 GBV_ILN_813 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2012 GBV_ILN_2018 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2083 GBV_ILN_2285 GBV_ILN_2497 GBV_ILN_2551 48.00 Land- und Forstwirtschaft: Allgemeines VZ AR 179 2015 1 0501 1-11 11 045F 580
spelling	10.1016/j.jplph.2015.02.007 doi GBVA2015006000024.pica (DE-627)ELV034454179 (ELSEVIER)S0176-1617(15)00047-4 DE-627 ger DE-627 rakwb eng 580 580 DE-600 610 VZ 630 640 VZ 48.00 bkl Navarro, Sara verfasserin aut Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2 2015transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage. Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage. Trehalose Elsevier Dehydrin gene expression Elsevier Cold storage Elsevier Dehydrin isoforms Elsevier Vitis vinifera Elsevier Vazquez-Hernandez, María oth Rosales, Raquel oth Sanchez-Ballesta, María Teresa oth Merodio, Carmen oth Escribano, María Isabel oth Enthalten in Elsevier Continuity of ambulatory care and health outcomes in adult patients with type 2 diabetes in Korea 2013 biochemistry, physiology, molecular biology and biotechnology of plants München (DE-627)ELV021744629 volume:179 year:2015 day:1 month:05 pages:1-11 extent:11 https://doi.org/10.1016/j.jplph.2015.02.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-FOR GBV_ILN_22 GBV_ILN_31 GBV_ILN_70 GBV_ILN_78 GBV_ILN_90 GBV_ILN_120 GBV_ILN_121 GBV_ILN_130 GBV_ILN_131 GBV_ILN_181 GBV_ILN_709 GBV_ILN_813 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2012 GBV_ILN_2018 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2083 GBV_ILN_2285 GBV_ILN_2497 GBV_ILN_2551 48.00 Land- und Forstwirtschaft: Allgemeines VZ AR 179 2015 1 0501 1-11 11 045F 580
allfields_unstemmed	10.1016/j.jplph.2015.02.007 doi GBVA2015006000024.pica (DE-627)ELV034454179 (ELSEVIER)S0176-1617(15)00047-4 DE-627 ger DE-627 rakwb eng 580 580 DE-600 610 VZ 630 640 VZ 48.00 bkl Navarro, Sara verfasserin aut Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2 2015transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage. Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage. Trehalose Elsevier Dehydrin gene expression Elsevier Cold storage Elsevier Dehydrin isoforms Elsevier Vitis vinifera Elsevier Vazquez-Hernandez, María oth Rosales, Raquel oth Sanchez-Ballesta, María Teresa oth Merodio, Carmen oth Escribano, María Isabel oth Enthalten in Elsevier Continuity of ambulatory care and health outcomes in adult patients with type 2 diabetes in Korea 2013 biochemistry, physiology, molecular biology and biotechnology of plants München (DE-627)ELV021744629 volume:179 year:2015 day:1 month:05 pages:1-11 extent:11 https://doi.org/10.1016/j.jplph.2015.02.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-FOR GBV_ILN_22 GBV_ILN_31 GBV_ILN_70 GBV_ILN_78 GBV_ILN_90 GBV_ILN_120 GBV_ILN_121 GBV_ILN_130 GBV_ILN_131 GBV_ILN_181 GBV_ILN_709 GBV_ILN_813 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2012 GBV_ILN_2018 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2083 GBV_ILN_2285 GBV_ILN_2497 GBV_ILN_2551 48.00 Land- und Forstwirtschaft: Allgemeines VZ AR 179 2015 1 0501 1-11 11 045F 580
allfieldsGer	10.1016/j.jplph.2015.02.007 doi GBVA2015006000024.pica (DE-627)ELV034454179 (ELSEVIER)S0176-1617(15)00047-4 DE-627 ger DE-627 rakwb eng 580 580 DE-600 610 VZ 630 640 VZ 48.00 bkl Navarro, Sara verfasserin aut Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2 2015transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage. Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage. Trehalose Elsevier Dehydrin gene expression Elsevier Cold storage Elsevier Dehydrin isoforms Elsevier Vitis vinifera Elsevier Vazquez-Hernandez, María oth Rosales, Raquel oth Sanchez-Ballesta, María Teresa oth Merodio, Carmen oth Escribano, María Isabel oth Enthalten in Elsevier Continuity of ambulatory care and health outcomes in adult patients with type 2 diabetes in Korea 2013 biochemistry, physiology, molecular biology and biotechnology of plants München (DE-627)ELV021744629 volume:179 year:2015 day:1 month:05 pages:1-11 extent:11 https://doi.org/10.1016/j.jplph.2015.02.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-FOR GBV_ILN_22 GBV_ILN_31 GBV_ILN_70 GBV_ILN_78 GBV_ILN_90 GBV_ILN_120 GBV_ILN_121 GBV_ILN_130 GBV_ILN_131 GBV_ILN_181 GBV_ILN_709 GBV_ILN_813 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2012 GBV_ILN_2018 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2083 GBV_ILN_2285 GBV_ILN_2497 GBV_ILN_2551 48.00 Land- und Forstwirtschaft: Allgemeines VZ AR 179 2015 1 0501 1-11 11 045F 580
allfieldsSound	10.1016/j.jplph.2015.02.007 doi GBVA2015006000024.pica (DE-627)ELV034454179 (ELSEVIER)S0176-1617(15)00047-4 DE-627 ger DE-627 rakwb eng 580 580 DE-600 610 VZ 630 640 VZ 48.00 bkl Navarro, Sara verfasserin aut Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2 2015transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage. Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage. Trehalose Elsevier Dehydrin gene expression Elsevier Cold storage Elsevier Dehydrin isoforms Elsevier Vitis vinifera Elsevier Vazquez-Hernandez, María oth Rosales, Raquel oth Sanchez-Ballesta, María Teresa oth Merodio, Carmen oth Escribano, María Isabel oth Enthalten in Elsevier Continuity of ambulatory care and health outcomes in adult patients with type 2 diabetes in Korea 2013 biochemistry, physiology, molecular biology and biotechnology of plants München (DE-627)ELV021744629 volume:179 year:2015 day:1 month:05 pages:1-11 extent:11 https://doi.org/10.1016/j.jplph.2015.02.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-FOR GBV_ILN_22 GBV_ILN_31 GBV_ILN_70 GBV_ILN_78 GBV_ILN_90 GBV_ILN_120 GBV_ILN_121 GBV_ILN_130 GBV_ILN_131 GBV_ILN_181 GBV_ILN_709 GBV_ILN_813 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2012 GBV_ILN_2018 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2083 GBV_ILN_2285 GBV_ILN_2497 GBV_ILN_2551 48.00 Land- und Forstwirtschaft: Allgemeines VZ AR 179 2015 1 0501 1-11 11 045F 580
language	English
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authorswithroles_txt_mv	Navarro, Sara @@aut@@ Vazquez-Hernandez, María @@oth@@ Rosales, Raquel @@oth@@ Sanchez-Ballesta, María Teresa @@oth@@ Merodio, Carmen @@oth@@ Escribano, María Isabel @@oth@@
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title_full	Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2
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title_sort	differential regulation of dehydrin expression and trehalose levels in cardinal table grape skin by low temperature and high co2
title_auth	Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2
abstract	Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage.
abstractGer	Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage.
abstract_unstemmed	Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage.
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title_short	Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2
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up_date	2024-07-06T21:09:58.836Z
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In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. We propose that the constitutive presence and up-regulation of dehydrins and trehalose during low temperature postharvest storage could be positively correlated with the relative chilling tolerance of table grapes and the adaptive responses activated by high CO2 levels to preserve cell water status and to counteract the disruption of physiological processes during cold storage.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Dehydrins and trehalose are multifunctional protective biomolecules that play a role in counteracting cellular damage during dehydrative stresses. In this paper, we studied dehydrin isoform patterns, dehydrin gene expression and trehalose levels in the skin of Cardinal (Vitis vinifera L.) table grapes, along with their regulation by different cold postharvest storage conditions. Immunoanalysis with K-segment antibody recognizes four constitutive dehydrins (from 17 to 44kDa) that are tightly regulated by low temperature and high CO2. Phosphatase treatment showed that DHN44 and DHN22 isoforms are phosphorylated polypeptides, while MALDI-TOF MS and MS/MS analysis suggested that 44kDa polypeptide may be a dehydrin homodimer. At the transcriptional level, dehydrins are also regulated by low temperature and high CO2, showing a fairly good correlation with their mRNA levels. Trehalose was quantified by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD), revealing a progressive increase of this metabolite throughout storage at 0°C and the sudden transitory increases in short-term high CO2-treated fruit. 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Differential regulation of dehydrin expression and trehalose levels in Cardinal table grape skin by low temperature and high CO2

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