High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification
• Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Odunuga, Odutayo O. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2015 |
|---|
| Schlagwörter: |
|---|
| Umfang: |
6 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake - Satriano, Claudio ELSEVIER, 2014, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:113 ; year:2015 ; pages:79-84 ; extent:6 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.pep.2015.05.005 |
|---|
| Katalog-ID: |
ELV034761950 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | ELV034761950 | ||
| 003 | DE-627 | ||
| 005 | 20230624015128.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180603s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.pep.2015.05.005 |2 doi | |
| 028 | 5 | 2 | |a GBVA2015018000021.pica |
| 035 | |a (DE-627)ELV034761950 | ||
| 035 | |a (ELSEVIER)S1046-5928(15)00110-2 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | |a 570 |a 610 | |
| 082 | 0 | 4 | |a 570 |q DE-600 |
| 082 | 0 | 4 | |a 610 |q DE-600 |
| 082 | 0 | 4 | |a 550 |q VZ |
| 082 | 0 | 4 | |a 610 |a 333.7 |q VZ |
| 084 | |a BIODIV |q DE-30 |2 fid | ||
| 084 | |a 42.90 |2 bkl | ||
| 084 | |a 42.11 |2 bkl | ||
| 100 | 1 | |a Odunuga, Odutayo O. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification |
| 264 | 1 | |c 2015 | |
| 300 | |a 6 | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a • Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed. | ||
| 650 | 7 | |a Precipitation |2 Elsevier | |
| 650 | 7 | |a Purification |2 Elsevier | |
| 650 | 7 | |a Refolding |2 Elsevier | |
| 650 | 7 | |a Elastase |2 Elsevier | |
| 650 | 7 | |a Over-expression |2 Elsevier | |
| 650 | 7 | |a Pseudolysin |2 Elsevier | |
| 700 | 1 | |a Adekoya, Olayiwola A. |4 oth | |
| 700 | 1 | |a Sylte, Ingebrigt |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier |a Satriano, Claudio ELSEVIER |t Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake |d 2014 |g Amsterdam [u.a.] |w (DE-627)ELV023049715 |
| 773 | 1 | 8 | |g volume:113 |g year:2015 |g pages:79-84 |g extent:6 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.pep.2015.05.005 |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a GBV_ELV | ||
| 912 | |a SYSFLAG_U | ||
| 912 | |a FID-BIODIV | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a GBV_ILN_31 | ||
| 912 | |a GBV_ILN_72 | ||
| 912 | |a GBV_ILN_217 | ||
| 912 | |a GBV_ILN_255 | ||
| 912 | |a GBV_ILN_257 | ||
| 912 | |a GBV_ILN_286 | ||
| 912 | |a GBV_ILN_341 | ||
| 936 | b | k | |a 42.90 |j Ökologie: Allgemeines |q VZ |
| 936 | b | k | |a 42.11 |j Biomathematik |j Biokybernetik |q VZ |
| 951 | |a AR | ||
| 952 | |d 113 |j 2015 |h 79-84 |g 6 | ||
| 953 | |2 045F |a 570 | ||
| author_variant |
o o o oo ooo |
|---|---|
| matchkey_str |
odunugaodutayooadekoyaolayiwolaasylteing:2015----:iheeepesoopedlsnhetaellrlsaefsuooaargnsiec |
| hierarchy_sort_str |
2015 |
| bklnumber |
42.90 42.11 |
| publishDate |
2015 |
| allfields |
10.1016/j.pep.2015.05.005 doi GBVA2015018000021.pica (DE-627)ELV034761950 (ELSEVIER)S1046-5928(15)00110-2 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl Odunuga, Odutayo O. verfasserin aut High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification 2015 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier • Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed. Precipitation Elsevier Purification Elsevier Refolding Elsevier Elastase Elsevier Over-expression Elsevier Pseudolysin Elsevier Adekoya, Olayiwola A. oth Sylte, Ingebrigt oth Enthalten in Elsevier Satriano, Claudio ELSEVIER Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake 2014 Amsterdam [u.a.] (DE-627)ELV023049715 volume:113 year:2015 pages:79-84 extent:6 https://doi.org/10.1016/j.pep.2015.05.005 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_31 GBV_ILN_72 GBV_ILN_217 GBV_ILN_255 GBV_ILN_257 GBV_ILN_286 GBV_ILN_341 42.90 Ökologie: Allgemeines VZ 42.11 Biomathematik Biokybernetik VZ AR 113 2015 79-84 6 045F 570 |
| spelling |
10.1016/j.pep.2015.05.005 doi GBVA2015018000021.pica (DE-627)ELV034761950 (ELSEVIER)S1046-5928(15)00110-2 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl Odunuga, Odutayo O. verfasserin aut High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification 2015 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier • Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed. Precipitation Elsevier Purification Elsevier Refolding Elsevier Elastase Elsevier Over-expression Elsevier Pseudolysin Elsevier Adekoya, Olayiwola A. oth Sylte, Ingebrigt oth Enthalten in Elsevier Satriano, Claudio ELSEVIER Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake 2014 Amsterdam [u.a.] (DE-627)ELV023049715 volume:113 year:2015 pages:79-84 extent:6 https://doi.org/10.1016/j.pep.2015.05.005 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_31 GBV_ILN_72 GBV_ILN_217 GBV_ILN_255 GBV_ILN_257 GBV_ILN_286 GBV_ILN_341 42.90 Ökologie: Allgemeines VZ 42.11 Biomathematik Biokybernetik VZ AR 113 2015 79-84 6 045F 570 |
| allfields_unstemmed |
10.1016/j.pep.2015.05.005 doi GBVA2015018000021.pica (DE-627)ELV034761950 (ELSEVIER)S1046-5928(15)00110-2 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl Odunuga, Odutayo O. verfasserin aut High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification 2015 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier • Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed. Precipitation Elsevier Purification Elsevier Refolding Elsevier Elastase Elsevier Over-expression Elsevier Pseudolysin Elsevier Adekoya, Olayiwola A. oth Sylte, Ingebrigt oth Enthalten in Elsevier Satriano, Claudio ELSEVIER Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake 2014 Amsterdam [u.a.] (DE-627)ELV023049715 volume:113 year:2015 pages:79-84 extent:6 https://doi.org/10.1016/j.pep.2015.05.005 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_31 GBV_ILN_72 GBV_ILN_217 GBV_ILN_255 GBV_ILN_257 GBV_ILN_286 GBV_ILN_341 42.90 Ökologie: Allgemeines VZ 42.11 Biomathematik Biokybernetik VZ AR 113 2015 79-84 6 045F 570 |
| allfieldsGer |
10.1016/j.pep.2015.05.005 doi GBVA2015018000021.pica (DE-627)ELV034761950 (ELSEVIER)S1046-5928(15)00110-2 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl Odunuga, Odutayo O. verfasserin aut High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification 2015 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier • Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed. Precipitation Elsevier Purification Elsevier Refolding Elsevier Elastase Elsevier Over-expression Elsevier Pseudolysin Elsevier Adekoya, Olayiwola A. oth Sylte, Ingebrigt oth Enthalten in Elsevier Satriano, Claudio ELSEVIER Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake 2014 Amsterdam [u.a.] (DE-627)ELV023049715 volume:113 year:2015 pages:79-84 extent:6 https://doi.org/10.1016/j.pep.2015.05.005 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_31 GBV_ILN_72 GBV_ILN_217 GBV_ILN_255 GBV_ILN_257 GBV_ILN_286 GBV_ILN_341 42.90 Ökologie: Allgemeines VZ 42.11 Biomathematik Biokybernetik VZ AR 113 2015 79-84 6 045F 570 |
| allfieldsSound |
10.1016/j.pep.2015.05.005 doi GBVA2015018000021.pica (DE-627)ELV034761950 (ELSEVIER)S1046-5928(15)00110-2 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl Odunuga, Odutayo O. verfasserin aut High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification 2015 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier • Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed. Precipitation Elsevier Purification Elsevier Refolding Elsevier Elastase Elsevier Over-expression Elsevier Pseudolysin Elsevier Adekoya, Olayiwola A. oth Sylte, Ingebrigt oth Enthalten in Elsevier Satriano, Claudio ELSEVIER Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake 2014 Amsterdam [u.a.] (DE-627)ELV023049715 volume:113 year:2015 pages:79-84 extent:6 https://doi.org/10.1016/j.pep.2015.05.005 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_31 GBV_ILN_72 GBV_ILN_217 GBV_ILN_255 GBV_ILN_257 GBV_ILN_286 GBV_ILN_341 42.90 Ökologie: Allgemeines VZ 42.11 Biomathematik Biokybernetik VZ AR 113 2015 79-84 6 045F 570 |
| language |
English |
| source |
Enthalten in Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake Amsterdam [u.a.] volume:113 year:2015 pages:79-84 extent:6 |
| sourceStr |
Enthalten in Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake Amsterdam [u.a.] volume:113 year:2015 pages:79-84 extent:6 |
| format_phy_str_mv |
Article |
| bklname |
Ökologie: Allgemeines Biomathematik Biokybernetik |
| institution |
findex.gbv.de |
| topic_facet |
Precipitation Purification Refolding Elastase Over-expression Pseudolysin |
| dewey-raw |
570 |
| isfreeaccess_bool |
false |
| container_title |
Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake |
| authorswithroles_txt_mv |
Odunuga, Odutayo O. @@aut@@ Adekoya, Olayiwola A. @@oth@@ Sylte, Ingebrigt @@oth@@ |
| publishDateDaySort_date |
2015-01-01T00:00:00Z |
| hierarchy_top_id |
ELV023049715 |
| dewey-sort |
3570 |
| id |
ELV034761950 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV034761950</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230624015128.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180603s2015 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.pep.2015.05.005</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBVA2015018000021.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV034761950</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S1046-5928(15)00110-2</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">570</subfield><subfield code="a">610</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">550</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="a">333.7</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIODIV</subfield><subfield code="q">DE-30</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.90</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.11</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Odunuga, Odutayo O.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">6</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">• Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Precipitation</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Purification</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Refolding</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Elastase</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Over-expression</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Pseudolysin</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Adekoya, Olayiwola A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sylte, Ingebrigt</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier</subfield><subfield code="a">Satriano, Claudio ELSEVIER</subfield><subfield code="t">Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake</subfield><subfield code="d">2014</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV023049715</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:113</subfield><subfield code="g">year:2015</subfield><subfield code="g">pages:79-84</subfield><subfield code="g">extent:6</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.pep.2015.05.005</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-BIODIV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_72</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_217</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_255</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_257</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_286</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_341</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">42.90</subfield><subfield code="j">Ökologie: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">42.11</subfield><subfield code="j">Biomathematik</subfield><subfield code="j">Biokybernetik</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">113</subfield><subfield code="j">2015</subfield><subfield code="h">79-84</subfield><subfield code="g">6</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">570</subfield></datafield></record></collection>
|
| author |
Odunuga, Odutayo O. |
| spellingShingle |
Odunuga, Odutayo O. ddc 570 ddc 610 ddc 550 fid BIODIV bkl 42.90 bkl 42.11 Elsevier Precipitation Elsevier Purification Elsevier Refolding Elsevier Elastase Elsevier Over-expression Elsevier Pseudolysin High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification |
| authorStr |
Odunuga, Odutayo O. |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)ELV023049715 |
| format |
electronic Article |
| dewey-ones |
570 - Life sciences; biology 610 - Medicine & health 550 - Earth sciences 333 - Economics of land & energy |
| delete_txt_mv |
keep |
| author_role |
aut |
| collection |
elsevier |
| remote_str |
true |
| illustrated |
Not Illustrated |
| topic_title |
570 610 570 DE-600 610 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification Precipitation Elsevier Purification Elsevier Refolding Elsevier Elastase Elsevier Over-expression Elsevier Pseudolysin Elsevier |
| topic |
ddc 570 ddc 610 ddc 550 fid BIODIV bkl 42.90 bkl 42.11 Elsevier Precipitation Elsevier Purification Elsevier Refolding Elsevier Elastase Elsevier Over-expression Elsevier Pseudolysin |
| topic_unstemmed |
ddc 570 ddc 610 ddc 550 fid BIODIV bkl 42.90 bkl 42.11 Elsevier Precipitation Elsevier Purification Elsevier Refolding Elsevier Elastase Elsevier Over-expression Elsevier Pseudolysin |
| topic_browse |
ddc 570 ddc 610 ddc 550 fid BIODIV bkl 42.90 bkl 42.11 Elsevier Precipitation Elsevier Purification Elsevier Refolding Elsevier Elastase Elsevier Over-expression Elsevier Pseudolysin |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
o a a oa oaa i s is |
| hierarchy_parent_title |
Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake |
| hierarchy_parent_id |
ELV023049715 |
| dewey-tens |
570 - Life sciences; biology 610 - Medicine & health 550 - Earth sciences & geology 330 - Economics |
| hierarchy_top_title |
Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)ELV023049715 |
| title |
High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification |
| ctrlnum |
(DE-627)ELV034761950 (ELSEVIER)S1046-5928(15)00110-2 |
| title_full |
High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification |
| author_sort |
Odunuga, Odutayo O. |
| journal |
Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake |
| journalStr |
Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
500 - Science 600 - Technology 300 - Social sciences |
| recordtype |
marc |
| publishDateSort |
2015 |
| contenttype_str_mv |
zzz |
| container_start_page |
79 |
| author_browse |
Odunuga, Odutayo O. |
| container_volume |
113 |
| physical |
6 |
| class |
570 610 570 DE-600 610 DE-600 550 VZ 610 333.7 VZ BIODIV DE-30 fid 42.90 bkl 42.11 bkl |
| format_se |
Elektronische Aufsätze |
| author-letter |
Odunuga, Odutayo O. |
| doi_str_mv |
10.1016/j.pep.2015.05.005 |
| dewey-full |
570 610 550 333.7 |
| title_sort |
high-level expression of pseudolysin, the extracellular elastase of pseudomonas aeruginosa, in escherichia coli and its purification |
| title_auth |
High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification |
| abstract |
• Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed. |
| abstractGer |
• Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed. |
| abstract_unstemmed |
• Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed. |
| collection_details |
GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_31 GBV_ILN_72 GBV_ILN_217 GBV_ILN_255 GBV_ILN_257 GBV_ILN_286 GBV_ILN_341 |
| title_short |
High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification |
| url |
https://doi.org/10.1016/j.pep.2015.05.005 |
| remote_bool |
true |
| author2 |
Adekoya, Olayiwola A. Sylte, Ingebrigt |
| author2Str |
Adekoya, Olayiwola A. Sylte, Ingebrigt |
| ppnlink |
ELV023049715 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth |
| doi_str |
10.1016/j.pep.2015.05.005 |
| up_date |
2024-07-06T21:56:28.193Z |
| _version_ |
1803868426149560320 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV034761950</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230624015128.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180603s2015 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.pep.2015.05.005</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBVA2015018000021.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV034761950</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S1046-5928(15)00110-2</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">570</subfield><subfield code="a">610</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">550</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="a">333.7</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIODIV</subfield><subfield code="q">DE-30</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.90</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.11</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Odunuga, Odutayo O.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">6</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">• Full-length pseudolysin was successfully expressed at high levels in Escherichia coli. • Full-length pseudolysin was purified in relatively large quantity. • Denatured pseudolysin was refolded correctly without zinc and calcium and shown to be catalytically active. • Precipitation during refolding of urea-denatured pseudolysin was eliminated. • A fast and reproducible scheme for expression and purification of pseudolysin was developed.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Precipitation</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Purification</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Refolding</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Elastase</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Over-expression</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Pseudolysin</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Adekoya, Olayiwola A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sylte, Ingebrigt</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier</subfield><subfield code="a">Satriano, Claudio ELSEVIER</subfield><subfield code="t">Structural and thermal control of seismic activity and megathrust rupture dynamics in subduction zones: Lessons from the Mw 9.0, 2011 Tohoku earthquake</subfield><subfield code="d">2014</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV023049715</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:113</subfield><subfield code="g">year:2015</subfield><subfield code="g">pages:79-84</subfield><subfield code="g">extent:6</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.pep.2015.05.005</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-BIODIV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_72</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_217</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_255</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_257</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_286</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_341</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">42.90</subfield><subfield code="j">Ökologie: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">42.11</subfield><subfield code="j">Biomathematik</subfield><subfield code="j">Biokybernetik</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">113</subfield><subfield code="j">2015</subfield><subfield code="h">79-84</subfield><subfield code="g">6</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">570</subfield></datafield></record></collection>
|
| score |
7.400732 |