Development of a sandwiched microarray platform for studying the interactions of antibiotics with Staphylococcus aureus
It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimize...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Liu, Xia [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016transfer abstract |
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| Schlagwörter: |
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| Umfang: |
8 |
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| Übergeordnetes Werk: |
Enthalten in: Neuro-Brucellosis - Gouider, R. ELSEVIER, 2015, an international journal devoted to all branches of analytical chemistry, Amsterdam |
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| Übergeordnetes Werk: |
volume:917 ; year:2016 ; day:21 ; month:04 ; pages:93-100 ; extent:8 |
| Links: |
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| DOI / URN: |
10.1016/j.aca.2016.02.038 |
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ELV035558482 |
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| 520 | |a It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. | ||
| 520 | |a It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. | ||
| 650 | 7 | |a Bacterium |2 Elsevier | |
| 650 | 7 | |a Antibiotics |2 Elsevier | |
| 650 | 7 | |a Lectin-hydrogel microarrays |2 Elsevier | |
| 650 | 7 | |a Sandwiched microarray platform |2 Elsevier | |
| 700 | 1 | |a Lei, Zhen |4 oth | |
| 700 | 1 | |a Liu, Dianjun |4 oth | |
| 700 | 1 | |a Wang, Zhenxin |4 oth | |
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10.1016/j.aca.2016.02.038 doi GBVA2016020000016.pica (DE-627)ELV035558482 (ELSEVIER)S0003-2670(16)30275-6 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 540 VZ 35.10 bkl Liu, Xia verfasserin aut Development of a sandwiched microarray platform for studying the interactions of antibiotics with Staphylococcus aureus 2016transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. Bacterium Elsevier Antibiotics Elsevier Lectin-hydrogel microarrays Elsevier Sandwiched microarray platform Elsevier Lei, Zhen oth Liu, Dianjun oth Wang, Zhenxin oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:917 year:2016 day:21 month:04 pages:93-100 extent:8 https://doi.org/10.1016/j.aca.2016.02.038 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 917 2016 21 0421 93-100 8 045F 540 |
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10.1016/j.aca.2016.02.038 doi GBVA2016020000016.pica (DE-627)ELV035558482 (ELSEVIER)S0003-2670(16)30275-6 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 540 VZ 35.10 bkl Liu, Xia verfasserin aut Development of a sandwiched microarray platform for studying the interactions of antibiotics with Staphylococcus aureus 2016transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. Bacterium Elsevier Antibiotics Elsevier Lectin-hydrogel microarrays Elsevier Sandwiched microarray platform Elsevier Lei, Zhen oth Liu, Dianjun oth Wang, Zhenxin oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:917 year:2016 day:21 month:04 pages:93-100 extent:8 https://doi.org/10.1016/j.aca.2016.02.038 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 917 2016 21 0421 93-100 8 045F 540 |
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10.1016/j.aca.2016.02.038 doi GBVA2016020000016.pica (DE-627)ELV035558482 (ELSEVIER)S0003-2670(16)30275-6 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 540 VZ 35.10 bkl Liu, Xia verfasserin aut Development of a sandwiched microarray platform for studying the interactions of antibiotics with Staphylococcus aureus 2016transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. Bacterium Elsevier Antibiotics Elsevier Lectin-hydrogel microarrays Elsevier Sandwiched microarray platform Elsevier Lei, Zhen oth Liu, Dianjun oth Wang, Zhenxin oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:917 year:2016 day:21 month:04 pages:93-100 extent:8 https://doi.org/10.1016/j.aca.2016.02.038 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 917 2016 21 0421 93-100 8 045F 540 |
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10.1016/j.aca.2016.02.038 doi GBVA2016020000016.pica (DE-627)ELV035558482 (ELSEVIER)S0003-2670(16)30275-6 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 540 VZ 35.10 bkl Liu, Xia verfasserin aut Development of a sandwiched microarray platform for studying the interactions of antibiotics with Staphylococcus aureus 2016transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. Bacterium Elsevier Antibiotics Elsevier Lectin-hydrogel microarrays Elsevier Sandwiched microarray platform Elsevier Lei, Zhen oth Liu, Dianjun oth Wang, Zhenxin oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:917 year:2016 day:21 month:04 pages:93-100 extent:8 https://doi.org/10.1016/j.aca.2016.02.038 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 917 2016 21 0421 93-100 8 045F 540 |
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10.1016/j.aca.2016.02.038 doi GBVA2016020000016.pica (DE-627)ELV035558482 (ELSEVIER)S0003-2670(16)30275-6 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 540 VZ 35.10 bkl Liu, Xia verfasserin aut Development of a sandwiched microarray platform for studying the interactions of antibiotics with Staphylococcus aureus 2016transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. Bacterium Elsevier Antibiotics Elsevier Lectin-hydrogel microarrays Elsevier Sandwiched microarray platform Elsevier Lei, Zhen oth Liu, Dianjun oth Wang, Zhenxin oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:917 year:2016 day:21 month:04 pages:93-100 extent:8 https://doi.org/10.1016/j.aca.2016.02.038 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 917 2016 21 0421 93-100 8 045F 540 |
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development of a sandwiched microarray platform for studying the interactions of antibiotics with staphylococcus aureus |
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Development of a sandwiched microarray platform for studying the interactions of antibiotics with Staphylococcus aureus |
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It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. |
| abstractGer |
It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. |
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It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus (S. aureus) and four well-known antibiotics (amoxicillin, vancomycin, streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin, vancomycin and streptomycin are 1.7 μg mL−1, 3.3 μg mL−1 and 10.3 μg mL−1, respectively. |
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