Graphene oxide nanosheets induced genotoxicity and pulmonary injury in mice
Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mic...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
El-Yamany, Nabil A. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017transfer abstract |
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| Schlagwörter: |
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| Umfang: |
10 |
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| Übergeordnetes Werk: |
Enthalten in: Calcium phosphate particulates increase friction in the rat knee joint - Roemhildt, M.L. ELSEVIER, 2014, official journal of the Gesellschaft für Toxikologische Pathologie, München |
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| Übergeordnetes Werk: |
volume:69 ; year:2017 ; number:6 ; day:5 ; month:07 ; pages:383-392 ; extent:10 |
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| DOI / URN: |
10.1016/j.etp.2017.03.002 |
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| 520 | |a Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. | ||
| 520 | |a Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. | ||
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| 773 | 0 | 8 | |i Enthalten in |n Elsevier |a Roemhildt, M.L. ELSEVIER |t Calcium phosphate particulates increase friction in the rat knee joint |d 2014 |d official journal of the Gesellschaft für Toxikologische Pathologie |g München |w (DE-627)ELV017871352 |
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10.1016/j.etp.2017.03.002 doi GBVA2017013000005.pica (DE-627)ELV035945206 (ELSEVIER)S0940-2993(17)30105-7 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 660 620 VZ 52.56 bkl El-Yamany, Nabil A. verfasserin aut Graphene oxide nanosheets induced genotoxicity and pulmonary injury in mice 2017transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. mM Elsevier DMSO Elsevier TNA Elsevier A549 Elsevier SCGE Elsevier GOs Elsevier Kv Elsevier MDA Elsevier XRD Elsevier Cu Elsevier °C Elsevier NaNO3 Elsevier LSD Elsevier ml Elsevier NBT Elsevier TSA Elsevier SWCNTs Elsevier i.p Elsevier TBARS Elsevier μg Elsevier i.v. Elsevier LaB6 Elsevier μl Elsevier α Elsevier μm Elsevier H&E Elsevier Min Elsevier JCPDS Elsevier GNFs Elsevier volt Elsevier CAT Elsevier ROS Elsevier NaCl Elsevier MWCNTs Elsevier SEM Elsevier BEAS-2B Elsevier nm Elsevier b.wt Elsevier G Elsevier NaOH Elsevier HR-TEM Elsevier HCl Elsevier SD Elsevier 2D Elsevier CAs Elsevier IBN Elsevier ANOVA Elsevier CNTs Elsevier U Elsevier H2O2 Elsevier V Elsevier pH Elsevier SPSS Elsevier GSH Elsevier DTNB Elsevier H2SO4 Elsevier gm Elsevier GO Elsevier h Elsevier SOD Elsevier EDTA Elsevier NIH Elsevier m Elsevier TIA Elsevier rpm Elsevier nmol Elsevier SCA Elsevier mA Elsevier DNA Elsevier Kg Elsevier TEM Elsevier KMnO4 Elsevier Mohamed, Faten F. oth Salaheldin, Taher A. oth Tohamy, Amany A. oth Abd El-Mohsen, Walaa N. oth Amin, Adel S. oth Enthalten in Elsevier Roemhildt, M.L. ELSEVIER Calcium phosphate particulates increase friction in the rat knee joint 2014 official journal of the Gesellschaft für Toxikologische Pathologie München (DE-627)ELV017871352 volume:69 year:2017 number:6 day:5 month:07 pages:383-392 extent:10 https://doi.org/10.1016/j.etp.2017.03.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2001 52.56 Regenerative Energieformen alternative Energieformen VZ AR 69 2017 6 5 0705 383-392 10 045F 610 |
| spelling |
10.1016/j.etp.2017.03.002 doi GBVA2017013000005.pica (DE-627)ELV035945206 (ELSEVIER)S0940-2993(17)30105-7 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 660 620 VZ 52.56 bkl El-Yamany, Nabil A. verfasserin aut Graphene oxide nanosheets induced genotoxicity and pulmonary injury in mice 2017transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. mM Elsevier DMSO Elsevier TNA Elsevier A549 Elsevier SCGE Elsevier GOs Elsevier Kv Elsevier MDA Elsevier XRD Elsevier Cu Elsevier °C Elsevier NaNO3 Elsevier LSD Elsevier ml Elsevier NBT Elsevier TSA Elsevier SWCNTs Elsevier i.p Elsevier TBARS Elsevier μg Elsevier i.v. Elsevier LaB6 Elsevier μl Elsevier α Elsevier μm Elsevier H&E Elsevier Min Elsevier JCPDS Elsevier GNFs Elsevier volt Elsevier CAT Elsevier ROS Elsevier NaCl Elsevier MWCNTs Elsevier SEM Elsevier BEAS-2B Elsevier nm Elsevier b.wt Elsevier G Elsevier NaOH Elsevier HR-TEM Elsevier HCl Elsevier SD Elsevier 2D Elsevier CAs Elsevier IBN Elsevier ANOVA Elsevier CNTs Elsevier U Elsevier H2O2 Elsevier V Elsevier pH Elsevier SPSS Elsevier GSH Elsevier DTNB Elsevier H2SO4 Elsevier gm Elsevier GO Elsevier h Elsevier SOD Elsevier EDTA Elsevier NIH Elsevier m Elsevier TIA Elsevier rpm Elsevier nmol Elsevier SCA Elsevier mA Elsevier DNA Elsevier Kg Elsevier TEM Elsevier KMnO4 Elsevier Mohamed, Faten F. oth Salaheldin, Taher A. oth Tohamy, Amany A. oth Abd El-Mohsen, Walaa N. oth Amin, Adel S. oth Enthalten in Elsevier Roemhildt, M.L. ELSEVIER Calcium phosphate particulates increase friction in the rat knee joint 2014 official journal of the Gesellschaft für Toxikologische Pathologie München (DE-627)ELV017871352 volume:69 year:2017 number:6 day:5 month:07 pages:383-392 extent:10 https://doi.org/10.1016/j.etp.2017.03.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2001 52.56 Regenerative Energieformen alternative Energieformen VZ AR 69 2017 6 5 0705 383-392 10 045F 610 |
| allfields_unstemmed |
10.1016/j.etp.2017.03.002 doi GBVA2017013000005.pica (DE-627)ELV035945206 (ELSEVIER)S0940-2993(17)30105-7 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 660 620 VZ 52.56 bkl El-Yamany, Nabil A. verfasserin aut Graphene oxide nanosheets induced genotoxicity and pulmonary injury in mice 2017transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. mM Elsevier DMSO Elsevier TNA Elsevier A549 Elsevier SCGE Elsevier GOs Elsevier Kv Elsevier MDA Elsevier XRD Elsevier Cu Elsevier °C Elsevier NaNO3 Elsevier LSD Elsevier ml Elsevier NBT Elsevier TSA Elsevier SWCNTs Elsevier i.p Elsevier TBARS Elsevier μg Elsevier i.v. Elsevier LaB6 Elsevier μl Elsevier α Elsevier μm Elsevier H&E Elsevier Min Elsevier JCPDS Elsevier GNFs Elsevier volt Elsevier CAT Elsevier ROS Elsevier NaCl Elsevier MWCNTs Elsevier SEM Elsevier BEAS-2B Elsevier nm Elsevier b.wt Elsevier G Elsevier NaOH Elsevier HR-TEM Elsevier HCl Elsevier SD Elsevier 2D Elsevier CAs Elsevier IBN Elsevier ANOVA Elsevier CNTs Elsevier U Elsevier H2O2 Elsevier V Elsevier pH Elsevier SPSS Elsevier GSH Elsevier DTNB Elsevier H2SO4 Elsevier gm Elsevier GO Elsevier h Elsevier SOD Elsevier EDTA Elsevier NIH Elsevier m Elsevier TIA Elsevier rpm Elsevier nmol Elsevier SCA Elsevier mA Elsevier DNA Elsevier Kg Elsevier TEM Elsevier KMnO4 Elsevier Mohamed, Faten F. oth Salaheldin, Taher A. oth Tohamy, Amany A. oth Abd El-Mohsen, Walaa N. oth Amin, Adel S. oth Enthalten in Elsevier Roemhildt, M.L. ELSEVIER Calcium phosphate particulates increase friction in the rat knee joint 2014 official journal of the Gesellschaft für Toxikologische Pathologie München (DE-627)ELV017871352 volume:69 year:2017 number:6 day:5 month:07 pages:383-392 extent:10 https://doi.org/10.1016/j.etp.2017.03.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2001 52.56 Regenerative Energieformen alternative Energieformen VZ AR 69 2017 6 5 0705 383-392 10 045F 610 |
| allfieldsGer |
10.1016/j.etp.2017.03.002 doi GBVA2017013000005.pica (DE-627)ELV035945206 (ELSEVIER)S0940-2993(17)30105-7 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 660 620 VZ 52.56 bkl El-Yamany, Nabil A. verfasserin aut Graphene oxide nanosheets induced genotoxicity and pulmonary injury in mice 2017transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. mM Elsevier DMSO Elsevier TNA Elsevier A549 Elsevier SCGE Elsevier GOs Elsevier Kv Elsevier MDA Elsevier XRD Elsevier Cu Elsevier °C Elsevier NaNO3 Elsevier LSD Elsevier ml Elsevier NBT Elsevier TSA Elsevier SWCNTs Elsevier i.p Elsevier TBARS Elsevier μg Elsevier i.v. Elsevier LaB6 Elsevier μl Elsevier α Elsevier μm Elsevier H&E Elsevier Min Elsevier JCPDS Elsevier GNFs Elsevier volt Elsevier CAT Elsevier ROS Elsevier NaCl Elsevier MWCNTs Elsevier SEM Elsevier BEAS-2B Elsevier nm Elsevier b.wt Elsevier G Elsevier NaOH Elsevier HR-TEM Elsevier HCl Elsevier SD Elsevier 2D Elsevier CAs Elsevier IBN Elsevier ANOVA Elsevier CNTs Elsevier U Elsevier H2O2 Elsevier V Elsevier pH Elsevier SPSS Elsevier GSH Elsevier DTNB Elsevier H2SO4 Elsevier gm Elsevier GO Elsevier h Elsevier SOD Elsevier EDTA Elsevier NIH Elsevier m Elsevier TIA Elsevier rpm Elsevier nmol Elsevier SCA Elsevier mA Elsevier DNA Elsevier Kg Elsevier TEM Elsevier KMnO4 Elsevier Mohamed, Faten F. oth Salaheldin, Taher A. oth Tohamy, Amany A. oth Abd El-Mohsen, Walaa N. oth Amin, Adel S. oth Enthalten in Elsevier Roemhildt, M.L. ELSEVIER Calcium phosphate particulates increase friction in the rat knee joint 2014 official journal of the Gesellschaft für Toxikologische Pathologie München (DE-627)ELV017871352 volume:69 year:2017 number:6 day:5 month:07 pages:383-392 extent:10 https://doi.org/10.1016/j.etp.2017.03.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2001 52.56 Regenerative Energieformen alternative Energieformen VZ AR 69 2017 6 5 0705 383-392 10 045F 610 |
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10.1016/j.etp.2017.03.002 doi GBVA2017013000005.pica (DE-627)ELV035945206 (ELSEVIER)S0940-2993(17)30105-7 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 660 620 VZ 52.56 bkl El-Yamany, Nabil A. verfasserin aut Graphene oxide nanosheets induced genotoxicity and pulmonary injury in mice 2017transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. mM Elsevier DMSO Elsevier TNA Elsevier A549 Elsevier SCGE Elsevier GOs Elsevier Kv Elsevier MDA Elsevier XRD Elsevier Cu Elsevier °C Elsevier NaNO3 Elsevier LSD Elsevier ml Elsevier NBT Elsevier TSA Elsevier SWCNTs Elsevier i.p Elsevier TBARS Elsevier μg Elsevier i.v. Elsevier LaB6 Elsevier μl Elsevier α Elsevier μm Elsevier H&E Elsevier Min Elsevier JCPDS Elsevier GNFs Elsevier volt Elsevier CAT Elsevier ROS Elsevier NaCl Elsevier MWCNTs Elsevier SEM Elsevier BEAS-2B Elsevier nm Elsevier b.wt Elsevier G Elsevier NaOH Elsevier HR-TEM Elsevier HCl Elsevier SD Elsevier 2D Elsevier CAs Elsevier IBN Elsevier ANOVA Elsevier CNTs Elsevier U Elsevier H2O2 Elsevier V Elsevier pH Elsevier SPSS Elsevier GSH Elsevier DTNB Elsevier H2SO4 Elsevier gm Elsevier GO Elsevier h Elsevier SOD Elsevier EDTA Elsevier NIH Elsevier m Elsevier TIA Elsevier rpm Elsevier nmol Elsevier SCA Elsevier mA Elsevier DNA Elsevier Kg Elsevier TEM Elsevier KMnO4 Elsevier Mohamed, Faten F. oth Salaheldin, Taher A. oth Tohamy, Amany A. oth Abd El-Mohsen, Walaa N. oth Amin, Adel S. oth Enthalten in Elsevier Roemhildt, M.L. ELSEVIER Calcium phosphate particulates increase friction in the rat knee joint 2014 official journal of the Gesellschaft für Toxikologische Pathologie München (DE-627)ELV017871352 volume:69 year:2017 number:6 day:5 month:07 pages:383-392 extent:10 https://doi.org/10.1016/j.etp.2017.03.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2001 52.56 Regenerative Energieformen alternative Energieformen VZ AR 69 2017 6 5 0705 383-392 10 045F 610 |
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Enthalten in Calcium phosphate particulates increase friction in the rat knee joint München volume:69 year:2017 number:6 day:5 month:07 pages:383-392 extent:10 |
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Enthalten in Calcium phosphate particulates increase friction in the rat knee joint München volume:69 year:2017 number:6 day:5 month:07 pages:383-392 extent:10 |
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Calcium phosphate particulates increase friction in the rat knee joint |
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El-Yamany, Nabil A. @@aut@@ Mohamed, Faten F. @@oth@@ Salaheldin, Taher A. @@oth@@ Tohamy, Amany A. @@oth@@ Abd El-Mohsen, Walaa N. @@oth@@ Amin, Adel S. @@oth@@ |
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This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. 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Graphene oxide nanosheets induced genotoxicity and pulmonary injury in mice |
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Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. |
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Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. |
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Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets’ (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury. Conclusion: GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner. |
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