A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes
High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Vienne, Jean-Claude [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017transfer abstract |
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| Umfang: |
6 |
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| Übergeordnetes Werk: |
Enthalten in: Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems - Kroon, Frederieke J. ELSEVIER, 2014, methods in the biological sciences, San Diego, Calif |
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| Übergeordnetes Werk: |
volume:528 ; year:2017 ; day:1 ; month:07 ; pages:57-62 ; extent:6 |
| Links: |
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| DOI / URN: |
10.1016/j.ab.2017.04.015 |
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ELV03611877X |
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| 245 | 1 | 0 | |a A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes |
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| 520 | |a High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. | ||
| 520 | |a High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. | ||
| 650 | 7 | |a Saponin-permeabilized fibers |2 Elsevier | |
| 650 | 7 | |a High resolution oxymetry |2 Elsevier | |
| 650 | 7 | |a Ultradispersion |2 Elsevier | |
| 650 | 7 | |a Electron transfer system (ETS) |2 Elsevier | |
| 650 | 7 | |a MIR05 buffer |2 Elsevier | |
| 650 | 7 | |a FCCP |2 Elsevier | |
| 650 | 7 | |a Muscle needle biopsy |2 Elsevier | |
| 650 | 7 | |a Respiratory chain complex |2 Elsevier | |
| 700 | 1 | |a Cimetta, Catherine |4 oth | |
| 700 | 1 | |a Dubois, Marie |4 oth | |
| 700 | 1 | |a Duburcq, Thibault |4 oth | |
| 700 | 1 | |a Favory, Raphaël |4 oth | |
| 700 | 1 | |a Dessein, Anne-Frédérique |4 oth | |
| 700 | 1 | |a Fontaine, Monique |4 oth | |
| 700 | 1 | |a Joncquel-Chevalier Curt, Marie |4 oth | |
| 700 | 1 | |a Cuisset, Jean-Marie |4 oth | |
| 700 | 1 | |a Douillard, Claire |4 oth | |
| 700 | 1 | |a Mention-Mulliez, Karine |4 oth | |
| 700 | 1 | |a Dobbelaere, Dries |4 oth | |
| 700 | 1 | |a Vamecq, Joseph |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier |a Kroon, Frederieke J. ELSEVIER |t Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems |d 2014 |d methods in the biological sciences |g San Diego, Calif |w (DE-627)ELV018040411 |
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10.1016/j.ab.2017.04.015 doi GBV00000000000076A.pica (DE-627)ELV03611877X (ELSEVIER)S0003-2697(17)30183-5 DE-627 ger DE-627 rakwb eng 570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Vienne, Jean-Claude verfasserin aut A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes 2017transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. Saponin-permeabilized fibers Elsevier High resolution oxymetry Elsevier Ultradispersion Elsevier Electron transfer system (ETS) Elsevier MIR05 buffer Elsevier FCCP Elsevier Muscle needle biopsy Elsevier Respiratory chain complex Elsevier Cimetta, Catherine oth Dubois, Marie oth Duburcq, Thibault oth Favory, Raphaël oth Dessein, Anne-Frédérique oth Fontaine, Monique oth Joncquel-Chevalier Curt, Marie oth Cuisset, Jean-Marie oth Douillard, Claire oth Mention-Mulliez, Karine oth Dobbelaere, Dries oth Vamecq, Joseph oth Enthalten in Elsevier Kroon, Frederieke J. ELSEVIER Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems 2014 methods in the biological sciences San Diego, Calif (DE-627)ELV018040411 volume:528 year:2017 day:1 month:07 pages:57-62 extent:6 https://doi.org/10.1016/j.ab.2017.04.015 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 528 2017 1 0701 57-62 6 045F 570 |
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10.1016/j.ab.2017.04.015 doi GBV00000000000076A.pica (DE-627)ELV03611877X (ELSEVIER)S0003-2697(17)30183-5 DE-627 ger DE-627 rakwb eng 570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Vienne, Jean-Claude verfasserin aut A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes 2017transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. Saponin-permeabilized fibers Elsevier High resolution oxymetry Elsevier Ultradispersion Elsevier Electron transfer system (ETS) Elsevier MIR05 buffer Elsevier FCCP Elsevier Muscle needle biopsy Elsevier Respiratory chain complex Elsevier Cimetta, Catherine oth Dubois, Marie oth Duburcq, Thibault oth Favory, Raphaël oth Dessein, Anne-Frédérique oth Fontaine, Monique oth Joncquel-Chevalier Curt, Marie oth Cuisset, Jean-Marie oth Douillard, Claire oth Mention-Mulliez, Karine oth Dobbelaere, Dries oth Vamecq, Joseph oth Enthalten in Elsevier Kroon, Frederieke J. ELSEVIER Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems 2014 methods in the biological sciences San Diego, Calif (DE-627)ELV018040411 volume:528 year:2017 day:1 month:07 pages:57-62 extent:6 https://doi.org/10.1016/j.ab.2017.04.015 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 528 2017 1 0701 57-62 6 045F 570 |
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10.1016/j.ab.2017.04.015 doi GBV00000000000076A.pica (DE-627)ELV03611877X (ELSEVIER)S0003-2697(17)30183-5 DE-627 ger DE-627 rakwb eng 570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Vienne, Jean-Claude verfasserin aut A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes 2017transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. Saponin-permeabilized fibers Elsevier High resolution oxymetry Elsevier Ultradispersion Elsevier Electron transfer system (ETS) Elsevier MIR05 buffer Elsevier FCCP Elsevier Muscle needle biopsy Elsevier Respiratory chain complex Elsevier Cimetta, Catherine oth Dubois, Marie oth Duburcq, Thibault oth Favory, Raphaël oth Dessein, Anne-Frédérique oth Fontaine, Monique oth Joncquel-Chevalier Curt, Marie oth Cuisset, Jean-Marie oth Douillard, Claire oth Mention-Mulliez, Karine oth Dobbelaere, Dries oth Vamecq, Joseph oth Enthalten in Elsevier Kroon, Frederieke J. ELSEVIER Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems 2014 methods in the biological sciences San Diego, Calif (DE-627)ELV018040411 volume:528 year:2017 day:1 month:07 pages:57-62 extent:6 https://doi.org/10.1016/j.ab.2017.04.015 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 528 2017 1 0701 57-62 6 045F 570 |
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10.1016/j.ab.2017.04.015 doi GBV00000000000076A.pica (DE-627)ELV03611877X (ELSEVIER)S0003-2697(17)30183-5 DE-627 ger DE-627 rakwb eng 570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Vienne, Jean-Claude verfasserin aut A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes 2017transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. Saponin-permeabilized fibers Elsevier High resolution oxymetry Elsevier Ultradispersion Elsevier Electron transfer system (ETS) Elsevier MIR05 buffer Elsevier FCCP Elsevier Muscle needle biopsy Elsevier Respiratory chain complex Elsevier Cimetta, Catherine oth Dubois, Marie oth Duburcq, Thibault oth Favory, Raphaël oth Dessein, Anne-Frédérique oth Fontaine, Monique oth Joncquel-Chevalier Curt, Marie oth Cuisset, Jean-Marie oth Douillard, Claire oth Mention-Mulliez, Karine oth Dobbelaere, Dries oth Vamecq, Joseph oth Enthalten in Elsevier Kroon, Frederieke J. ELSEVIER Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems 2014 methods in the biological sciences San Diego, Calif (DE-627)ELV018040411 volume:528 year:2017 day:1 month:07 pages:57-62 extent:6 https://doi.org/10.1016/j.ab.2017.04.015 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 528 2017 1 0701 57-62 6 045F 570 |
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10.1016/j.ab.2017.04.015 doi GBV00000000000076A.pica (DE-627)ELV03611877X (ELSEVIER)S0003-2697(17)30183-5 DE-627 ger DE-627 rakwb eng 570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Vienne, Jean-Claude verfasserin aut A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes 2017transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. Saponin-permeabilized fibers Elsevier High resolution oxymetry Elsevier Ultradispersion Elsevier Electron transfer system (ETS) Elsevier MIR05 buffer Elsevier FCCP Elsevier Muscle needle biopsy Elsevier Respiratory chain complex Elsevier Cimetta, Catherine oth Dubois, Marie oth Duburcq, Thibault oth Favory, Raphaël oth Dessein, Anne-Frédérique oth Fontaine, Monique oth Joncquel-Chevalier Curt, Marie oth Cuisset, Jean-Marie oth Douillard, Claire oth Mention-Mulliez, Karine oth Dobbelaere, Dries oth Vamecq, Joseph oth Enthalten in Elsevier Kroon, Frederieke J. ELSEVIER Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems 2014 methods in the biological sciences San Diego, Calif (DE-627)ELV018040411 volume:528 year:2017 day:1 month:07 pages:57-62 extent:6 https://doi.org/10.1016/j.ab.2017.04.015 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 528 2017 1 0701 57-62 6 045F 570 |
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Enthalten in Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems San Diego, Calif volume:528 year:2017 day:1 month:07 pages:57-62 extent:6 |
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Enthalten in Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems San Diego, Calif volume:528 year:2017 day:1 month:07 pages:57-62 extent:6 |
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Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems |
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Vienne, Jean-Claude @@aut@@ Cimetta, Catherine @@oth@@ Dubois, Marie @@oth@@ Duburcq, Thibault @@oth@@ Favory, Raphaël @@oth@@ Dessein, Anne-Frédérique @@oth@@ Fontaine, Monique @@oth@@ Joncquel-Chevalier Curt, Marie @@oth@@ Cuisset, Jean-Marie @@oth@@ Douillard, Claire @@oth@@ Mention-Mulliez, Karine @@oth@@ Dobbelaere, Dries @@oth@@ Vamecq, Joseph @@oth@@ |
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A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes |
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Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems |
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a fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes |
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A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes |
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High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. |
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High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. |
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High resolution oxymetry study (HROS) of skeletal muscle usually requires 90–120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively. This simplified procedure compares favorably with classical (permeabilized fibers) HROS in terms of respiratory chain complex activities. Mitochondria from cells undergoing ultradispersion were functionally preserved as attested by relative inefficacy of added cytochrome C (not crossing intact mitochondrial outer membrane) to stimulate mitochondrial respiration. Responsiveness of respiration to ADP (in the absence of FCCP) suggested that these intact mitochondria were outside cells disrupted by ultradispersion or within cells permeated by this procedure. |
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