Subcutaneous and intranasal immunization with Stx2B–Tir–Stx1B–Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice
The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the h...
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2011transfer abstract |
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| Umfang: |
7 |
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| Übergeordnetes Werk: |
Enthalten in: Quantifying contributions of leaf area and longevity to leaf area duration under increased planting density and nitrogen input regimens during maize yield improvement - Li, Yaoyao ELSEVIER, 2022, Amsterdam |
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| Übergeordnetes Werk: |
volume:29 ; year:2011 ; number:22 ; day:17 ; month:05 ; pages:3923-3929 ; extent:7 |
| Links: |
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| DOI / URN: |
10.1016/j.vaccine.2011.02.007 |
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ELV036711306 |
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| 520 | |a The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. | ||
| 520 | |a The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. | ||
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10.1016/j.vaccine.2011.02.007 doi GBVA2011008000016.pica (DE-627)ELV036711306 (ELSEVIER)S0264-410X(11)00208-8 DE-627 ger DE-627 rakwb eng 610 610 DE-600 630 640 VZ 48.00 bkl Subcutaneous and intranasal immunization with Stx2B–Tir–Stx1B–Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice 2011transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. Enthalten in Elsevier Li, Yaoyao ELSEVIER Quantifying contributions of leaf area and longevity to leaf area duration under increased planting density and nitrogen input regimens during maize yield improvement 2022 Amsterdam (DE-627)ELV007884451 volume:29 year:2011 number:22 day:17 month:05 pages:3923-3929 extent:7 https://doi.org/10.1016/j.vaccine.2011.02.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-FOR 48.00 Land- und Forstwirtschaft: Allgemeines VZ AR 29 2011 22 17 0517 3923-3929 7 045F 610 |
| spelling |
10.1016/j.vaccine.2011.02.007 doi GBVA2011008000016.pica (DE-627)ELV036711306 (ELSEVIER)S0264-410X(11)00208-8 DE-627 ger DE-627 rakwb eng 610 610 DE-600 630 640 VZ 48.00 bkl Subcutaneous and intranasal immunization with Stx2B–Tir–Stx1B–Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice 2011transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. Enthalten in Elsevier Li, Yaoyao ELSEVIER Quantifying contributions of leaf area and longevity to leaf area duration under increased planting density and nitrogen input regimens during maize yield improvement 2022 Amsterdam (DE-627)ELV007884451 volume:29 year:2011 number:22 day:17 month:05 pages:3923-3929 extent:7 https://doi.org/10.1016/j.vaccine.2011.02.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-FOR 48.00 Land- und Forstwirtschaft: Allgemeines VZ AR 29 2011 22 17 0517 3923-3929 7 045F 610 |
| allfields_unstemmed |
10.1016/j.vaccine.2011.02.007 doi GBVA2011008000016.pica (DE-627)ELV036711306 (ELSEVIER)S0264-410X(11)00208-8 DE-627 ger DE-627 rakwb eng 610 610 DE-600 630 640 VZ 48.00 bkl Subcutaneous and intranasal immunization with Stx2B–Tir–Stx1B–Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice 2011transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. Enthalten in Elsevier Li, Yaoyao ELSEVIER Quantifying contributions of leaf area and longevity to leaf area duration under increased planting density and nitrogen input regimens during maize yield improvement 2022 Amsterdam (DE-627)ELV007884451 volume:29 year:2011 number:22 day:17 month:05 pages:3923-3929 extent:7 https://doi.org/10.1016/j.vaccine.2011.02.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-FOR 48.00 Land- und Forstwirtschaft: Allgemeines VZ AR 29 2011 22 17 0517 3923-3929 7 045F 610 |
| allfieldsGer |
10.1016/j.vaccine.2011.02.007 doi GBVA2011008000016.pica (DE-627)ELV036711306 (ELSEVIER)S0264-410X(11)00208-8 DE-627 ger DE-627 rakwb eng 610 610 DE-600 630 640 VZ 48.00 bkl Subcutaneous and intranasal immunization with Stx2B–Tir–Stx1B–Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice 2011transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. Enthalten in Elsevier Li, Yaoyao ELSEVIER Quantifying contributions of leaf area and longevity to leaf area duration under increased planting density and nitrogen input regimens during maize yield improvement 2022 Amsterdam (DE-627)ELV007884451 volume:29 year:2011 number:22 day:17 month:05 pages:3923-3929 extent:7 https://doi.org/10.1016/j.vaccine.2011.02.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-FOR 48.00 Land- und Forstwirtschaft: Allgemeines VZ AR 29 2011 22 17 0517 3923-3929 7 045F 610 |
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10.1016/j.vaccine.2011.02.007 doi GBVA2011008000016.pica (DE-627)ELV036711306 (ELSEVIER)S0264-410X(11)00208-8 DE-627 ger DE-627 rakwb eng 610 610 DE-600 630 640 VZ 48.00 bkl Subcutaneous and intranasal immunization with Stx2B–Tir–Stx1B–Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice 2011transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. Enthalten in Elsevier Li, Yaoyao ELSEVIER Quantifying contributions of leaf area and longevity to leaf area duration under increased planting density and nitrogen input regimens during maize yield improvement 2022 Amsterdam (DE-627)ELV007884451 volume:29 year:2011 number:22 day:17 month:05 pages:3923-3929 extent:7 https://doi.org/10.1016/j.vaccine.2011.02.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-FOR 48.00 Land- und Forstwirtschaft: Allgemeines VZ AR 29 2011 22 17 0517 3923-3929 7 045F 610 |
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Enthalten in Quantifying contributions of leaf area and longevity to leaf area duration under increased planting density and nitrogen input regimens during maize yield improvement Amsterdam volume:29 year:2011 number:22 day:17 month:05 pages:3923-3929 extent:7 |
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Enthalten in Quantifying contributions of leaf area and longevity to leaf area duration under increased planting density and nitrogen input regimens during maize yield improvement Amsterdam volume:29 year:2011 number:22 day:17 month:05 pages:3923-3929 extent:7 |
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Quantifying contributions of leaf area and longevity to leaf area duration under increased planting density and nitrogen input regimens during maize yield improvement |
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Subcutaneous and intranasal immunization with Stx2B–Tir–Stx1B–Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice |
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Quantifying contributions of leaf area and longevity to leaf area duration under increased planting density and nitrogen input regimens during maize yield improvement |
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subcutaneous and intranasal immunization with stx2b–tir–stx1b–zot reduces colonization and shedding of escherichia coli o157:h7 in mice |
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Subcutaneous and intranasal immunization with Stx2B–Tir–Stx1B–Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice |
| abstract |
The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. |
| abstractGer |
The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. |
| abstract_unstemmed |
The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B–Tir–Stx1B–Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B–Tir–Stx1B–Zot, Stx2B–Tir–Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B–Tir–Stx1B–Zot induced significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B–Tir–Stx1B–Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B–Tir–Stx1B–Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B–Tir–Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B–Tir–Stx1B–Zot or Stx2B–Tir–Stx1B both showed reduced shedding in feces, moreover, Stx2B–Tir–Stx1B–Zot did better. These results demonstrate the perspective for the use of Stx2B–Tir–Stx1B–Zot to prevent colonization and shedding of E. coli O157:H7. |
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Subcutaneous and intranasal immunization with Stx2B–Tir–Stx1B–Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice |
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