Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus
Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Godfroy, Olivier [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015transfer abstract |
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| Umfang: |
5 |
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| Übergeordnetes Werk: |
Enthalten in: 95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses - 2012, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:24 ; year:2015 ; pages:109-113 ; extent:5 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.margen.2015.03.007 |
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ELV039762963 |
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| 520 | |a Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). | ||
| 520 | |a Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). | ||
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10.1016/j.margen.2015.03.007 doi GBVA2015011000007.pica (DE-627)ELV039762963 (ELSEVIER)S1874-7787(15)00047-1 DE-627 ger DE-627 rakwb eng 570 550 570 DE-600 550 DE-600 610 VZ 670 VZ 35.80 bkl Godfroy, Olivier verfasserin aut Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus 2015transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). Peters, Akira F. oth Coelho, Susana M. oth Cock, J. Mark oth Enthalten in Elsevier 95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses 2012 Amsterdam [u.a.] (DE-627)ELV016099354 volume:24 year:2015 pages:109-113 extent:5 https://doi.org/10.1016/j.margen.2015.03.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_30 GBV_ILN_70 GBV_ILN_100 35.80 Makromolekulare Chemie VZ AR 24 2015 109-113 5 045F 570 |
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10.1016/j.margen.2015.03.007 doi GBVA2015011000007.pica (DE-627)ELV039762963 (ELSEVIER)S1874-7787(15)00047-1 DE-627 ger DE-627 rakwb eng 570 550 570 DE-600 550 DE-600 610 VZ 670 VZ 35.80 bkl Godfroy, Olivier verfasserin aut Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus 2015transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). Peters, Akira F. oth Coelho, Susana M. oth Cock, J. Mark oth Enthalten in Elsevier 95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses 2012 Amsterdam [u.a.] (DE-627)ELV016099354 volume:24 year:2015 pages:109-113 extent:5 https://doi.org/10.1016/j.margen.2015.03.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_30 GBV_ILN_70 GBV_ILN_100 35.80 Makromolekulare Chemie VZ AR 24 2015 109-113 5 045F 570 |
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10.1016/j.margen.2015.03.007 doi GBVA2015011000007.pica (DE-627)ELV039762963 (ELSEVIER)S1874-7787(15)00047-1 DE-627 ger DE-627 rakwb eng 570 550 570 DE-600 550 DE-600 610 VZ 670 VZ 35.80 bkl Godfroy, Olivier verfasserin aut Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus 2015transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). Peters, Akira F. oth Coelho, Susana M. oth Cock, J. Mark oth Enthalten in Elsevier 95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses 2012 Amsterdam [u.a.] (DE-627)ELV016099354 volume:24 year:2015 pages:109-113 extent:5 https://doi.org/10.1016/j.margen.2015.03.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_30 GBV_ILN_70 GBV_ILN_100 35.80 Makromolekulare Chemie VZ AR 24 2015 109-113 5 045F 570 |
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10.1016/j.margen.2015.03.007 doi GBVA2015011000007.pica (DE-627)ELV039762963 (ELSEVIER)S1874-7787(15)00047-1 DE-627 ger DE-627 rakwb eng 570 550 570 DE-600 550 DE-600 610 VZ 670 VZ 35.80 bkl Godfroy, Olivier verfasserin aut Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus 2015transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). Peters, Akira F. oth Coelho, Susana M. oth Cock, J. Mark oth Enthalten in Elsevier 95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses 2012 Amsterdam [u.a.] (DE-627)ELV016099354 volume:24 year:2015 pages:109-113 extent:5 https://doi.org/10.1016/j.margen.2015.03.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_30 GBV_ILN_70 GBV_ILN_100 35.80 Makromolekulare Chemie VZ AR 24 2015 109-113 5 045F 570 |
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10.1016/j.margen.2015.03.007 doi GBVA2015011000007.pica (DE-627)ELV039762963 (ELSEVIER)S1874-7787(15)00047-1 DE-627 ger DE-627 rakwb eng 570 550 570 DE-600 550 DE-600 610 VZ 670 VZ 35.80 bkl Godfroy, Olivier verfasserin aut Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus 2015transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). Peters, Akira F. oth Coelho, Susana M. oth Cock, J. Mark oth Enthalten in Elsevier 95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses 2012 Amsterdam [u.a.] (DE-627)ELV016099354 volume:24 year:2015 pages:109-113 extent:5 https://doi.org/10.1016/j.margen.2015.03.007 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_30 GBV_ILN_70 GBV_ILN_100 35.80 Makromolekulare Chemie VZ AR 24 2015 109-113 5 045F 570 |
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Enthalten in 95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses Amsterdam [u.a.] volume:24 year:2015 pages:109-113 extent:5 |
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95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses |
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95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses |
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Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus |
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Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus |
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Godfroy, Olivier |
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95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses |
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95 Staging lymphadenectomy in renal cell carcinoma must be extended: A sensitivity curve analyses |
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10.1016/j.margen.2015.03.007 |
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genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga ectocarpus |
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Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus |
| abstract |
Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). |
| abstractGer |
Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). |
| abstract_unstemmed |
Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). |
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Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus |
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https://doi.org/10.1016/j.margen.2015.03.007 |
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