Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors
The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of vari...
Ausführliche Beschreibung
Autor*in: |
Vanderpool, Darin [verfasserIn] |
---|
Format: |
E-Artikel |
---|---|
Sprache: |
Englisch |
Erschienen: |
2015transfer abstract |
---|
Schlagwörter: |
---|
Umfang: |
7 |
---|
Übergeordnetes Werk: |
Enthalten in: Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems - Kroon, Frederieke J. ELSEVIER, 2014, methods in the biological sciences, San Diego, Calif |
---|---|
Übergeordnetes Werk: |
volume:473 ; year:2015 ; day:15 ; month:03 ; pages:46-52 ; extent:7 |
Links: |
---|
DOI / URN: |
10.1016/j.ab.2014.12.008 |
---|
Katalog-ID: |
ELV039904148 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | ELV039904148 | ||
003 | DE-627 | ||
005 | 20230625230234.0 | ||
007 | cr uuu---uuuuu | ||
008 | 180603s2015 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.ab.2014.12.008 |2 doi | |
028 | 5 | 2 | |a GBVA2015019000030.pica |
035 | |a (DE-627)ELV039904148 | ||
035 | |a (ELSEVIER)S0003-2697(14)00568-5 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | |a 570 |a 540 | |
082 | 0 | 4 | |a 570 |q DE-600 |
082 | 0 | 4 | |a 540 |q DE-600 |
082 | 0 | 4 | |a 550 |q VZ |
082 | 0 | 4 | |a 333.7 |q VZ |
082 | 0 | 4 | |a 610 |q VZ |
084 | |a 15,3 |2 ssgn | ||
084 | |a PHARM |q DE-84 |2 fid | ||
084 | |a 44.40 |2 bkl | ||
100 | 1 | |a Vanderpool, Darin |e verfasserin |4 aut | |
245 | 1 | 0 | |a Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors |
264 | 1 | |c 2015transfer abstract | |
300 | |a 7 | ||
336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
337 | |a nicht spezifiziert |b z |2 rdamedia | ||
338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
520 | |a The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. | ||
520 | |a The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. | ||
650 | 7 | |a ERK2 |2 Elsevier | |
650 | 7 | |a Time-dependent inhibition |2 Elsevier | |
650 | 7 | |a Residence time |2 Elsevier | |
650 | 7 | |a Kinetic efficiency |2 Elsevier | |
650 | 7 | |a Off-rate |2 Elsevier | |
650 | 7 | |a Structure–kinetic relationship |2 Elsevier | |
700 | 1 | |a Grimshaw, Charles E. |4 oth | |
700 | 1 | |a Lawson, J. David |4 oth | |
700 | 1 | |a Ermolieff, Jacques |4 oth | |
773 | 0 | 8 | |i Enthalten in |n Elsevier |a Kroon, Frederieke J. ELSEVIER |t Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems |d 2014 |d methods in the biological sciences |g San Diego, Calif |w (DE-627)ELV018040411 |
773 | 1 | 8 | |g volume:473 |g year:2015 |g day:15 |g month:03 |g pages:46-52 |g extent:7 |
856 | 4 | 0 | |u https://doi.org/10.1016/j.ab.2014.12.008 |3 Volltext |
912 | |a GBV_USEFLAG_U | ||
912 | |a GBV_ELV | ||
912 | |a SYSFLAG_U | ||
912 | |a FID-PHARM | ||
912 | |a SSG-OLC-PHA | ||
912 | |a SSG-OPC-PHA | ||
936 | b | k | |a 44.40 |j Pharmazie |j Pharmazeutika |q VZ |
951 | |a AR | ||
952 | |d 473 |j 2015 |b 15 |c 0315 |h 46-52 |g 7 | ||
953 | |2 045F |a 570 |
author_variant |
d v dv |
---|---|
matchkey_str |
vanderpooldaringrimshawcharleselawsonjda:2015----:eiectmadieiefcecaayioetaellrinleu |
hierarchy_sort_str |
2015transfer abstract |
bklnumber |
44.40 |
publishDate |
2015 |
allfields |
10.1016/j.ab.2014.12.008 doi GBVA2015019000030.pica (DE-627)ELV039904148 (ELSEVIER)S0003-2697(14)00568-5 DE-627 ger DE-627 rakwb eng 570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Vanderpool, Darin verfasserin aut Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. ERK2 Elsevier Time-dependent inhibition Elsevier Residence time Elsevier Kinetic efficiency Elsevier Off-rate Elsevier Structure–kinetic relationship Elsevier Grimshaw, Charles E. oth Lawson, J. David oth Ermolieff, Jacques oth Enthalten in Elsevier Kroon, Frederieke J. ELSEVIER Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems 2014 methods in the biological sciences San Diego, Calif (DE-627)ELV018040411 volume:473 year:2015 day:15 month:03 pages:46-52 extent:7 https://doi.org/10.1016/j.ab.2014.12.008 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 473 2015 15 0315 46-52 7 045F 570 |
spelling |
10.1016/j.ab.2014.12.008 doi GBVA2015019000030.pica (DE-627)ELV039904148 (ELSEVIER)S0003-2697(14)00568-5 DE-627 ger DE-627 rakwb eng 570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Vanderpool, Darin verfasserin aut Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. ERK2 Elsevier Time-dependent inhibition Elsevier Residence time Elsevier Kinetic efficiency Elsevier Off-rate Elsevier Structure–kinetic relationship Elsevier Grimshaw, Charles E. oth Lawson, J. David oth Ermolieff, Jacques oth Enthalten in Elsevier Kroon, Frederieke J. ELSEVIER Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems 2014 methods in the biological sciences San Diego, Calif (DE-627)ELV018040411 volume:473 year:2015 day:15 month:03 pages:46-52 extent:7 https://doi.org/10.1016/j.ab.2014.12.008 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 473 2015 15 0315 46-52 7 045F 570 |
allfields_unstemmed |
10.1016/j.ab.2014.12.008 doi GBVA2015019000030.pica (DE-627)ELV039904148 (ELSEVIER)S0003-2697(14)00568-5 DE-627 ger DE-627 rakwb eng 570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Vanderpool, Darin verfasserin aut Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. ERK2 Elsevier Time-dependent inhibition Elsevier Residence time Elsevier Kinetic efficiency Elsevier Off-rate Elsevier Structure–kinetic relationship Elsevier Grimshaw, Charles E. oth Lawson, J. David oth Ermolieff, Jacques oth Enthalten in Elsevier Kroon, Frederieke J. ELSEVIER Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems 2014 methods in the biological sciences San Diego, Calif (DE-627)ELV018040411 volume:473 year:2015 day:15 month:03 pages:46-52 extent:7 https://doi.org/10.1016/j.ab.2014.12.008 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 473 2015 15 0315 46-52 7 045F 570 |
allfieldsGer |
10.1016/j.ab.2014.12.008 doi GBVA2015019000030.pica (DE-627)ELV039904148 (ELSEVIER)S0003-2697(14)00568-5 DE-627 ger DE-627 rakwb eng 570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Vanderpool, Darin verfasserin aut Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. ERK2 Elsevier Time-dependent inhibition Elsevier Residence time Elsevier Kinetic efficiency Elsevier Off-rate Elsevier Structure–kinetic relationship Elsevier Grimshaw, Charles E. oth Lawson, J. David oth Ermolieff, Jacques oth Enthalten in Elsevier Kroon, Frederieke J. ELSEVIER Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems 2014 methods in the biological sciences San Diego, Calif (DE-627)ELV018040411 volume:473 year:2015 day:15 month:03 pages:46-52 extent:7 https://doi.org/10.1016/j.ab.2014.12.008 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 473 2015 15 0315 46-52 7 045F 570 |
allfieldsSound |
10.1016/j.ab.2014.12.008 doi GBVA2015019000030.pica (DE-627)ELV039904148 (ELSEVIER)S0003-2697(14)00568-5 DE-627 ger DE-627 rakwb eng 570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Vanderpool, Darin verfasserin aut Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. ERK2 Elsevier Time-dependent inhibition Elsevier Residence time Elsevier Kinetic efficiency Elsevier Off-rate Elsevier Structure–kinetic relationship Elsevier Grimshaw, Charles E. oth Lawson, J. David oth Ermolieff, Jacques oth Enthalten in Elsevier Kroon, Frederieke J. ELSEVIER Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems 2014 methods in the biological sciences San Diego, Calif (DE-627)ELV018040411 volume:473 year:2015 day:15 month:03 pages:46-52 extent:7 https://doi.org/10.1016/j.ab.2014.12.008 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 473 2015 15 0315 46-52 7 045F 570 |
language |
English |
source |
Enthalten in Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems San Diego, Calif volume:473 year:2015 day:15 month:03 pages:46-52 extent:7 |
sourceStr |
Enthalten in Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems San Diego, Calif volume:473 year:2015 day:15 month:03 pages:46-52 extent:7 |
format_phy_str_mv |
Article |
bklname |
Pharmazie Pharmazeutika |
institution |
findex.gbv.de |
topic_facet |
ERK2 Time-dependent inhibition Residence time Kinetic efficiency Off-rate Structure–kinetic relationship |
dewey-raw |
570 |
isfreeaccess_bool |
false |
container_title |
Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems |
authorswithroles_txt_mv |
Vanderpool, Darin @@aut@@ Grimshaw, Charles E. @@oth@@ Lawson, J. David @@oth@@ Ermolieff, Jacques @@oth@@ |
publishDateDaySort_date |
2015-01-15T00:00:00Z |
hierarchy_top_id |
ELV018040411 |
dewey-sort |
3570 |
id |
ELV039904148 |
language_de |
englisch |
fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV039904148</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230625230234.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180603s2015 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.ab.2014.12.008</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBVA2015019000030.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV039904148</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0003-2697(14)00568-5</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">570</subfield><subfield code="a">540</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">540</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">550</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">333.7</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">15,3</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">PHARM</subfield><subfield code="q">DE-84</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.40</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Vanderpool, Darin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">ERK2</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Time-dependent inhibition</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Residence time</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Kinetic efficiency</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Off-rate</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Structure–kinetic relationship</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Grimshaw, Charles E.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lawson, J. David</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ermolieff, Jacques</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier</subfield><subfield code="a">Kroon, Frederieke J. ELSEVIER</subfield><subfield code="t">Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems</subfield><subfield code="d">2014</subfield><subfield code="d">methods in the biological sciences</subfield><subfield code="g">San Diego, Calif</subfield><subfield code="w">(DE-627)ELV018040411</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:473</subfield><subfield code="g">year:2015</subfield><subfield code="g">day:15</subfield><subfield code="g">month:03</subfield><subfield code="g">pages:46-52</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.ab.2014.12.008</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-PHARM</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-PHA</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.40</subfield><subfield code="j">Pharmazie</subfield><subfield code="j">Pharmazeutika</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">473</subfield><subfield code="j">2015</subfield><subfield code="b">15</subfield><subfield code="c">0315</subfield><subfield code="h">46-52</subfield><subfield code="g">7</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">570</subfield></datafield></record></collection>
|
author |
Vanderpool, Darin |
spellingShingle |
Vanderpool, Darin ddc 570 ddc 540 ddc 550 ddc 333.7 ddc 610 ssgn 15,3 fid PHARM bkl 44.40 Elsevier ERK2 Elsevier Time-dependent inhibition Elsevier Residence time Elsevier Kinetic efficiency Elsevier Off-rate Elsevier Structure–kinetic relationship Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors |
authorStr |
Vanderpool, Darin |
ppnlink_with_tag_str_mv |
@@773@@(DE-627)ELV018040411 |
format |
electronic Article |
dewey-ones |
570 - Life sciences; biology 540 - Chemistry & allied sciences 550 - Earth sciences 333 - Economics of land & energy 610 - Medicine & health |
delete_txt_mv |
keep |
author_role |
aut |
collection |
elsevier |
remote_str |
true |
illustrated |
Not Illustrated |
topic_title |
570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors ERK2 Elsevier Time-dependent inhibition Elsevier Residence time Elsevier Kinetic efficiency Elsevier Off-rate Elsevier Structure–kinetic relationship Elsevier |
topic |
ddc 570 ddc 540 ddc 550 ddc 333.7 ddc 610 ssgn 15,3 fid PHARM bkl 44.40 Elsevier ERK2 Elsevier Time-dependent inhibition Elsevier Residence time Elsevier Kinetic efficiency Elsevier Off-rate Elsevier Structure–kinetic relationship |
topic_unstemmed |
ddc 570 ddc 540 ddc 550 ddc 333.7 ddc 610 ssgn 15,3 fid PHARM bkl 44.40 Elsevier ERK2 Elsevier Time-dependent inhibition Elsevier Residence time Elsevier Kinetic efficiency Elsevier Off-rate Elsevier Structure–kinetic relationship |
topic_browse |
ddc 570 ddc 540 ddc 550 ddc 333.7 ddc 610 ssgn 15,3 fid PHARM bkl 44.40 Elsevier ERK2 Elsevier Time-dependent inhibition Elsevier Residence time Elsevier Kinetic efficiency Elsevier Off-rate Elsevier Structure–kinetic relationship |
format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
format_main_str_mv |
Text Zeitschrift/Artikel |
carriertype_str_mv |
zu |
author2_variant |
c e g ce ceg j d l jd jdl j e je |
hierarchy_parent_title |
Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems |
hierarchy_parent_id |
ELV018040411 |
dewey-tens |
570 - Life sciences; biology 540 - Chemistry 550 - Earth sciences & geology 330 - Economics 610 - Medicine & health |
hierarchy_top_title |
Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems |
isfreeaccess_txt |
false |
familylinks_str_mv |
(DE-627)ELV018040411 |
title |
Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors |
ctrlnum |
(DE-627)ELV039904148 (ELSEVIER)S0003-2697(14)00568-5 |
title_full |
Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors |
author_sort |
Vanderpool, Darin |
journal |
Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems |
journalStr |
Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems |
lang_code |
eng |
isOA_bool |
false |
dewey-hundreds |
500 - Science 300 - Social sciences 600 - Technology |
recordtype |
marc |
publishDateSort |
2015 |
contenttype_str_mv |
zzz |
container_start_page |
46 |
author_browse |
Vanderpool, Darin |
container_volume |
473 |
physical |
7 |
class |
570 540 570 DE-600 540 DE-600 550 VZ 333.7 VZ 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl |
format_se |
Elektronische Aufsätze |
author-letter |
Vanderpool, Darin |
doi_str_mv |
10.1016/j.ab.2014.12.008 |
dewey-full |
570 540 550 333.7 610 |
title_sort |
residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors |
title_auth |
Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors |
abstract |
The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. |
abstractGer |
The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. |
abstract_unstemmed |
The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2. |
collection_details |
GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA |
title_short |
Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors |
url |
https://doi.org/10.1016/j.ab.2014.12.008 |
remote_bool |
true |
author2 |
Grimshaw, Charles E. Lawson, J. David Ermolieff, Jacques |
author2Str |
Grimshaw, Charles E. Lawson, J. David Ermolieff, Jacques |
ppnlink |
ELV018040411 |
mediatype_str_mv |
z |
isOA_txt |
false |
hochschulschrift_bool |
false |
author2_role |
oth oth oth |
doi_str |
10.1016/j.ab.2014.12.008 |
up_date |
2024-07-06T21:47:31.458Z |
_version_ |
1803867863342120960 |
fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV039904148</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230625230234.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180603s2015 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.ab.2014.12.008</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBVA2015019000030.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV039904148</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0003-2697(14)00568-5</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">570</subfield><subfield code="a">540</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">540</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">550</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">333.7</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">15,3</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">PHARM</subfield><subfield code="q">DE-84</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.40</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Vanderpool, Darin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The RAS/RAF/MEK/ERK signal transduction cascade plays an important role in the regulation of critical cellular processes such as cell proliferation, migration, and differentiation. The up-regulation of this pathway can negatively affect cell homeostasis and is responsible for the development of various forms of cancer and inflammation processes. Therefore, there is a strong interest in pursuing drug programs targeting some of the enzymes involved in this pathway. In addition to the determination of K i, K d, IC50, and/or EC50, a more thorough kinetic analysis can provide useful information for the selection of the best lead series during the early stage of the drug discovery process. This study describes a medium-throughput fluorescent probe displacement assay for the rapid determination of the k off constant, residence time, and kinetic efficiency for ERK (extracellular signal-regulated kinase) inhibitors. Using this method, we have identified several inhibitors that we have subjected to further kinetic analysis by comparing k off constants determined for these time-dependent inhibitors using either the active or inactive form of ERK2.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">ERK2</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Time-dependent inhibition</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Residence time</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Kinetic efficiency</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Off-rate</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Structure–kinetic relationship</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Grimshaw, Charles E.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lawson, J. David</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ermolieff, Jacques</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier</subfield><subfield code="a">Kroon, Frederieke J. ELSEVIER</subfield><subfield code="t">Informing policy to protect coastal coral reefs: Insight from a global review of reducing agricultural pollution to coastal ecosystems</subfield><subfield code="d">2014</subfield><subfield code="d">methods in the biological sciences</subfield><subfield code="g">San Diego, Calif</subfield><subfield code="w">(DE-627)ELV018040411</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:473</subfield><subfield code="g">year:2015</subfield><subfield code="g">day:15</subfield><subfield code="g">month:03</subfield><subfield code="g">pages:46-52</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.ab.2014.12.008</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-PHARM</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-PHA</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.40</subfield><subfield code="j">Pharmazie</subfield><subfield code="j">Pharmazeutika</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">473</subfield><subfield code="j">2015</subfield><subfield code="b">15</subfield><subfield code="c">0315</subfield><subfield code="h">46-52</subfield><subfield code="g">7</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">570</subfield></datafield></record></collection>
|
score |
7.400339 |