Multiplex immunoassays using virus-tethered gold microspheres by DC impedance-based flow cytometry
Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing...
Ausführliche Beschreibung
Autor*in: |
Rho, Jihun [verfasserIn] |
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Englisch |
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2018transfer abstract |
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8 |
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Enthalten in: Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines - Costanigro, Marco ELSEVIER, 2019, the principal international journal devoted to research, design development and application of biosensors and bioelectronics, Amsterdam [u.a.] |
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Übergeordnetes Werk: |
volume:102 ; year:2018 ; day:15 ; month:04 ; pages:121-128 ; extent:8 |
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DOI / URN: |
10.1016/j.bios.2017.11.027 |
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ELV041650727 |
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520 | |a Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. | ||
520 | |a Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. | ||
650 | 7 | |a Bead-based immunoassay |2 Elsevier | |
650 | 7 | |a DC impedance-based cytometer |2 Elsevier | |
650 | 7 | |a Filamentous bacteriophage |2 Elsevier | |
650 | 7 | |a Microfluidics |2 Elsevier | |
650 | 7 | |a Multiplex analysis |2 Elsevier | |
700 | 1 | |a Jang, Woohyuk |4 oth | |
700 | 1 | |a Hwang, Inseong |4 oth | |
700 | 1 | |a Lee, Dahye |4 oth | |
700 | 1 | |a Lee, Chang Heon |4 oth | |
700 | 1 | |a Chung, Taek Dong |4 oth | |
773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Costanigro, Marco ELSEVIER |t Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines |d 2019 |d the principal international journal devoted to research, design development and application of biosensors and bioelectronics |g Amsterdam [u.a.] |w (DE-627)ELV001931067 |
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10.1016/j.bios.2017.11.027 doi GBV00000000000361.pica (DE-627)ELV041650727 (ELSEVIER)S0956-5663(17)30755-8 DE-627 ger DE-627 rakwb eng 630 640 VZ 49.00 bkl Rho, Jihun verfasserin aut Multiplex immunoassays using virus-tethered gold microspheres by DC impedance-based flow cytometry 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. Bead-based immunoassay Elsevier DC impedance-based cytometer Elsevier Filamentous bacteriophage Elsevier Microfluidics Elsevier Multiplex analysis Elsevier Jang, Woohyuk oth Hwang, Inseong oth Lee, Dahye oth Lee, Chang Heon oth Chung, Taek Dong oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:102 year:2018 day:15 month:04 pages:121-128 extent:8 https://doi.org/10.1016/j.bios.2017.11.027 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 102 2018 15 0415 121-128 8 |
spelling |
10.1016/j.bios.2017.11.027 doi GBV00000000000361.pica (DE-627)ELV041650727 (ELSEVIER)S0956-5663(17)30755-8 DE-627 ger DE-627 rakwb eng 630 640 VZ 49.00 bkl Rho, Jihun verfasserin aut Multiplex immunoassays using virus-tethered gold microspheres by DC impedance-based flow cytometry 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. Bead-based immunoassay Elsevier DC impedance-based cytometer Elsevier Filamentous bacteriophage Elsevier Microfluidics Elsevier Multiplex analysis Elsevier Jang, Woohyuk oth Hwang, Inseong oth Lee, Dahye oth Lee, Chang Heon oth Chung, Taek Dong oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:102 year:2018 day:15 month:04 pages:121-128 extent:8 https://doi.org/10.1016/j.bios.2017.11.027 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 102 2018 15 0415 121-128 8 |
allfields_unstemmed |
10.1016/j.bios.2017.11.027 doi GBV00000000000361.pica (DE-627)ELV041650727 (ELSEVIER)S0956-5663(17)30755-8 DE-627 ger DE-627 rakwb eng 630 640 VZ 49.00 bkl Rho, Jihun verfasserin aut Multiplex immunoassays using virus-tethered gold microspheres by DC impedance-based flow cytometry 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. Bead-based immunoassay Elsevier DC impedance-based cytometer Elsevier Filamentous bacteriophage Elsevier Microfluidics Elsevier Multiplex analysis Elsevier Jang, Woohyuk oth Hwang, Inseong oth Lee, Dahye oth Lee, Chang Heon oth Chung, Taek Dong oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:102 year:2018 day:15 month:04 pages:121-128 extent:8 https://doi.org/10.1016/j.bios.2017.11.027 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 102 2018 15 0415 121-128 8 |
allfieldsGer |
10.1016/j.bios.2017.11.027 doi GBV00000000000361.pica (DE-627)ELV041650727 (ELSEVIER)S0956-5663(17)30755-8 DE-627 ger DE-627 rakwb eng 630 640 VZ 49.00 bkl Rho, Jihun verfasserin aut Multiplex immunoassays using virus-tethered gold microspheres by DC impedance-based flow cytometry 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. Bead-based immunoassay Elsevier DC impedance-based cytometer Elsevier Filamentous bacteriophage Elsevier Microfluidics Elsevier Multiplex analysis Elsevier Jang, Woohyuk oth Hwang, Inseong oth Lee, Dahye oth Lee, Chang Heon oth Chung, Taek Dong oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:102 year:2018 day:15 month:04 pages:121-128 extent:8 https://doi.org/10.1016/j.bios.2017.11.027 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 102 2018 15 0415 121-128 8 |
allfieldsSound |
10.1016/j.bios.2017.11.027 doi GBV00000000000361.pica (DE-627)ELV041650727 (ELSEVIER)S0956-5663(17)30755-8 DE-627 ger DE-627 rakwb eng 630 640 VZ 49.00 bkl Rho, Jihun verfasserin aut Multiplex immunoassays using virus-tethered gold microspheres by DC impedance-based flow cytometry 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. Bead-based immunoassay Elsevier DC impedance-based cytometer Elsevier Filamentous bacteriophage Elsevier Microfluidics Elsevier Multiplex analysis Elsevier Jang, Woohyuk oth Hwang, Inseong oth Lee, Dahye oth Lee, Chang Heon oth Chung, Taek Dong oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:102 year:2018 day:15 month:04 pages:121-128 extent:8 https://doi.org/10.1016/j.bios.2017.11.027 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 102 2018 15 0415 121-128 8 |
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Enthalten in Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines Amsterdam [u.a.] volume:102 year:2018 day:15 month:04 pages:121-128 extent:8 |
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Enthalten in Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines Amsterdam [u.a.] volume:102 year:2018 day:15 month:04 pages:121-128 extent:8 |
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Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines |
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Multiplex immunoassays using virus-tethered gold microspheres by DC impedance-based flow cytometry |
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Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. |
abstractGer |
Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. |
abstract_unstemmed |
Bead-based multiplex immunoassays for common use require enhanced sensitivity and effective prevention of non-specific adsorption, as well as miniaturization of the detection device. In this work, we have implemented virus-tethered gold microspheres for multiplex immunoassay applications, employing a DC impedance-based flow cytometer as a detection element. The advantages of virus-tethered gold microspheres, including excellent prevention of non-specific adsorption, are extended to signal enhancement arising from the large quantity of antibody loading on each virion, and to flexible movement of filamentous virus. Individual virus-tethered beads generate their own DC impedance and fluorescence signals, which are simultaneously detected by a chip-based microfluidic flow cytometer. This system successfully realized multiplex immunoassays involving four biomarkers: cardiac troponin I (cTnI), prostate specific antigen (PSA), creatine kinase MB (CK-MB), and myoglobin in undiluted human sera, elevating sensitivity by up to 5.7-fold compared to the beads without virus. Constructive integration between filamentous virus-tethered Au-layered microspheres and use of a microfluidic cytometer suggests a promising strategy for competitive multiplex immunoassay development based on suspension arrays. |
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Multiplex immunoassays using virus-tethered gold microspheres by DC impedance-based flow cytometry |
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