Transcriptomic approach: A promising tool for rapid screening nanomaterial-mediated toxicity in the marine bivalve Mytilus edulis—Application to copper oxide nanoparticles
The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Châtel, Amélie [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018transfer abstract |
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| Umfang: |
8 |
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| Übergeordnetes Werk: |
Enthalten in: Monitoring land surface albedo and vegetation dynamics using high spatial and temporal resolution synthetic time series from Landsat and the MODIS BRDF/NBAR/albedo product - Wang, Zhuosen ELSEVIER, 2017, CBP : an international journal, London [u.a.] |
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| Übergeordnetes Werk: |
volume:205 ; year:2018 ; pages:26-33 ; extent:8 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.cbpc.2018.01.003 |
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ELV042020212 |
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| 245 | 1 | 0 | |a Transcriptomic approach: A promising tool for rapid screening nanomaterial-mediated toxicity in the marine bivalve Mytilus edulis—Application to copper oxide nanoparticles |
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| 520 | |a The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. | ||
| 520 | |a The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. | ||
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| 700 | 1 | |a Bruneau, Mélanie |4 oth | |
| 700 | 1 | |a Mouneyrac, Catherine |4 oth | |
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10.1016/j.cbpc.2018.01.003 doi GBV00000000000141A.pica (DE-627)ELV042020212 (ELSEVIER)S1532-0456(18)30003-6 DE-627 ger DE-627 rakwb eng 610 610 DE-600 550 VZ KARTEN DE-1a fid 38.03 bkl 74.48 bkl 74.41 bkl Châtel, Amélie verfasserin aut Transcriptomic approach: A promising tool for rapid screening nanomaterial-mediated toxicity in the marine bivalve Mytilus edulis—Application to copper oxide nanoparticles 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. Lièvre, Clémence oth Barrick, Andrew oth Bruneau, Mélanie oth Mouneyrac, Catherine oth Enthalten in Pergamon Press Wang, Zhuosen ELSEVIER Monitoring land surface albedo and vegetation dynamics using high spatial and temporal resolution synthetic time series from Landsat and the MODIS BRDF/NBAR/albedo product 2017 CBP : an international journal London [u.a.] (DE-627)ELV007784783 volume:205 year:2018 pages:26-33 extent:8 https://doi.org/10.1016/j.cbpc.2018.01.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-KARTEN SSG-OPC-GGO SSG-OPC-AST SSG-OPC-GEO 38.03 Methoden und Techniken der Geowissenschaften VZ 74.48 Geoinformationssysteme VZ 74.41 Luftaufnahmen Photogrammetrie VZ AR 205 2018 26-33 8 045F 610 |
| spelling |
10.1016/j.cbpc.2018.01.003 doi GBV00000000000141A.pica (DE-627)ELV042020212 (ELSEVIER)S1532-0456(18)30003-6 DE-627 ger DE-627 rakwb eng 610 610 DE-600 550 VZ KARTEN DE-1a fid 38.03 bkl 74.48 bkl 74.41 bkl Châtel, Amélie verfasserin aut Transcriptomic approach: A promising tool for rapid screening nanomaterial-mediated toxicity in the marine bivalve Mytilus edulis—Application to copper oxide nanoparticles 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. Lièvre, Clémence oth Barrick, Andrew oth Bruneau, Mélanie oth Mouneyrac, Catherine oth Enthalten in Pergamon Press Wang, Zhuosen ELSEVIER Monitoring land surface albedo and vegetation dynamics using high spatial and temporal resolution synthetic time series from Landsat and the MODIS BRDF/NBAR/albedo product 2017 CBP : an international journal London [u.a.] (DE-627)ELV007784783 volume:205 year:2018 pages:26-33 extent:8 https://doi.org/10.1016/j.cbpc.2018.01.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-KARTEN SSG-OPC-GGO SSG-OPC-AST SSG-OPC-GEO 38.03 Methoden und Techniken der Geowissenschaften VZ 74.48 Geoinformationssysteme VZ 74.41 Luftaufnahmen Photogrammetrie VZ AR 205 2018 26-33 8 045F 610 |
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10.1016/j.cbpc.2018.01.003 doi GBV00000000000141A.pica (DE-627)ELV042020212 (ELSEVIER)S1532-0456(18)30003-6 DE-627 ger DE-627 rakwb eng 610 610 DE-600 550 VZ KARTEN DE-1a fid 38.03 bkl 74.48 bkl 74.41 bkl Châtel, Amélie verfasserin aut Transcriptomic approach: A promising tool for rapid screening nanomaterial-mediated toxicity in the marine bivalve Mytilus edulis—Application to copper oxide nanoparticles 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. Lièvre, Clémence oth Barrick, Andrew oth Bruneau, Mélanie oth Mouneyrac, Catherine oth Enthalten in Pergamon Press Wang, Zhuosen ELSEVIER Monitoring land surface albedo and vegetation dynamics using high spatial and temporal resolution synthetic time series from Landsat and the MODIS BRDF/NBAR/albedo product 2017 CBP : an international journal London [u.a.] (DE-627)ELV007784783 volume:205 year:2018 pages:26-33 extent:8 https://doi.org/10.1016/j.cbpc.2018.01.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-KARTEN SSG-OPC-GGO SSG-OPC-AST SSG-OPC-GEO 38.03 Methoden und Techniken der Geowissenschaften VZ 74.48 Geoinformationssysteme VZ 74.41 Luftaufnahmen Photogrammetrie VZ AR 205 2018 26-33 8 045F 610 |
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10.1016/j.cbpc.2018.01.003 doi GBV00000000000141A.pica (DE-627)ELV042020212 (ELSEVIER)S1532-0456(18)30003-6 DE-627 ger DE-627 rakwb eng 610 610 DE-600 550 VZ KARTEN DE-1a fid 38.03 bkl 74.48 bkl 74.41 bkl Châtel, Amélie verfasserin aut Transcriptomic approach: A promising tool for rapid screening nanomaterial-mediated toxicity in the marine bivalve Mytilus edulis—Application to copper oxide nanoparticles 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. Lièvre, Clémence oth Barrick, Andrew oth Bruneau, Mélanie oth Mouneyrac, Catherine oth Enthalten in Pergamon Press Wang, Zhuosen ELSEVIER Monitoring land surface albedo and vegetation dynamics using high spatial and temporal resolution synthetic time series from Landsat and the MODIS BRDF/NBAR/albedo product 2017 CBP : an international journal London [u.a.] (DE-627)ELV007784783 volume:205 year:2018 pages:26-33 extent:8 https://doi.org/10.1016/j.cbpc.2018.01.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-KARTEN SSG-OPC-GGO SSG-OPC-AST SSG-OPC-GEO 38.03 Methoden und Techniken der Geowissenschaften VZ 74.48 Geoinformationssysteme VZ 74.41 Luftaufnahmen Photogrammetrie VZ AR 205 2018 26-33 8 045F 610 |
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10.1016/j.cbpc.2018.01.003 doi GBV00000000000141A.pica (DE-627)ELV042020212 (ELSEVIER)S1532-0456(18)30003-6 DE-627 ger DE-627 rakwb eng 610 610 DE-600 550 VZ KARTEN DE-1a fid 38.03 bkl 74.48 bkl 74.41 bkl Châtel, Amélie verfasserin aut Transcriptomic approach: A promising tool for rapid screening nanomaterial-mediated toxicity in the marine bivalve Mytilus edulis—Application to copper oxide nanoparticles 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. Lièvre, Clémence oth Barrick, Andrew oth Bruneau, Mélanie oth Mouneyrac, Catherine oth Enthalten in Pergamon Press Wang, Zhuosen ELSEVIER Monitoring land surface albedo and vegetation dynamics using high spatial and temporal resolution synthetic time series from Landsat and the MODIS BRDF/NBAR/albedo product 2017 CBP : an international journal London [u.a.] (DE-627)ELV007784783 volume:205 year:2018 pages:26-33 extent:8 https://doi.org/10.1016/j.cbpc.2018.01.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-KARTEN SSG-OPC-GGO SSG-OPC-AST SSG-OPC-GEO 38.03 Methoden und Techniken der Geowissenschaften VZ 74.48 Geoinformationssysteme VZ 74.41 Luftaufnahmen Photogrammetrie VZ AR 205 2018 26-33 8 045F 610 |
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Enthalten in Monitoring land surface albedo and vegetation dynamics using high spatial and temporal resolution synthetic time series from Landsat and the MODIS BRDF/NBAR/albedo product London [u.a.] volume:205 year:2018 pages:26-33 extent:8 |
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transcriptomic approach: a promising tool for rapid screening nanomaterial-mediated toxicity in the marine bivalve mytilus edulis—application to copper oxide nanoparticles |
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Transcriptomic approach: A promising tool for rapid screening nanomaterial-mediated toxicity in the marine bivalve Mytilus edulis—Application to copper oxide nanoparticles |
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The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. |
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The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. |
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The extensive development of nanotechnologies will inevitably lead to the release of nanomaterials (NMs) in the environment. As the aquatic environments represent the ultimate sink for various contaminants, it is highly probable that they also constitute a reservoir for NMs and hence aquatic animals represent potential targets. In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves. |
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Transcriptomic approach: A promising tool for rapid screening nanomaterial-mediated toxicity in the marine bivalve Mytilus edulis—Application to copper oxide nanoparticles |
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In a regulatory perspective, it is necessary to develop tools to rapidly screen the impact of NMs on model organisms, given that the number of NMs on the market will be increasing. In this context High Throughput Screening approaches represent relevant tools for the investigation of NM-mediated toxicity. The objective of this work was to study the effects of copper oxide nanoparticles (CuONPs) in the marine bivalve Mytilus edulis, using a transcriptomic approach. Mussels were exposed in vivo to CuONPs (10 μg·L−1CuO NPs) for 24 h and analysis of mRNA expression levels of genes implicated in immune response, antioxidant activities, cell metabolism, cell transport and cytoskeleton was investigated by qPCR on hemocytes and gills. Results showed common effects of CuONPs and its ionic counterpart. However, greater effects of CuONPs on GST, SOD, MT, Actin, ATP synthase gene expressions were observed compared to ionic form indicating that toxicity of CuONPs is not solely due to the release of Cu2+. Even though M. edulis genome is not fully characterized, this study provides additional knowledge on the signaling pathways implicated in CuONP-mediated toxicity and demonstrates the reliability of using a qPCR approach to go further in the cellular aspects implicated in response to NPs in marine bivalves.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lièvre, Clémence</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Barrick, Andrew</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bruneau, Mélanie</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mouneyrac, Catherine</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Pergamon Press</subfield><subfield code="a">Wang, Zhuosen ELSEVIER</subfield><subfield code="t">Monitoring land surface albedo and vegetation dynamics using high spatial and temporal resolution synthetic time series from Landsat and the MODIS BRDF/NBAR/albedo product</subfield><subfield code="d">2017</subfield><subfield code="d">CBP : an international journal</subfield><subfield code="g">London [u.a.]</subfield><subfield code="w">(DE-627)ELV007784783</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:205</subfield><subfield code="g">year:2018</subfield><subfield code="g">pages:26-33</subfield><subfield code="g">extent:8</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.cbpc.2018.01.003</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-KARTEN</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-GGO</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-AST</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-GEO</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">38.03</subfield><subfield code="j">Methoden und Techniken der Geowissenschaften</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">74.48</subfield><subfield code="j">Geoinformationssysteme</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">74.41</subfield><subfield code="j">Luftaufnahmen</subfield><subfield code="j">Photogrammetrie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">205</subfield><subfield code="j">2018</subfield><subfield code="h">26-33</subfield><subfield code="g">8</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">610</subfield></datafield></record></collection>
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