Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic>

Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the a...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Liu, Yue [verfasserIn] Yang, Huiping Torres, Leticia Tiersch, Terrence R.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018transfer abstract

Umfang:	11

Übergeordnetes Werk:	Enthalten in: Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar - Brown, Kerry A. ELSEVIER, 2013, CBP : an internat. journal, Tarrytown, NY
Übergeordnetes Werk:	volume:218 ; year:2018 ; pages:35-45 ; extent:11

Links:	Volltext

DOI / URN:	10.1016/j.cbpa.2018.01.006

Katalog-ID:	ELV042103622

Internformat


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245	1	0	\|a Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic>
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520			\|a Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids.
520			\|a Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids.
700	1		\|a Yang, Huiping \|4 oth
700	1		\|a Torres, Leticia \|4 oth
700	1		\|a Tiersch, Terrence R. \|4 oth
773	0	8	\|i Enthalten in \|n Elsevier \|a Brown, Kerry A. ELSEVIER \|t Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar \|d 2013 \|d CBP : an internat. journal \|g Tarrytown, NY \|w (DE-627)ELV022192689
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author_variant	y l yl
matchkey_str	liuyueyanghuipingtorresleticiatierschter:2018----:ciainfrepradiscainfprbnlspraoegaafnnagrdiiaosi
hierarchy_sort_str	2018transfer abstract
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publishDate	2018
allfields	10.1016/j.cbpa.2018.01.006 doi GBV00000000000529.pica (DE-627)ELV042103622 (ELSEVIER)S1095-6433(18)30011-4 DE-627 ger DE-627 rakwb eng 570 VZ 540 VZ 610 VZ 630 VZ 22 ssgn 46.00 bkl Liu, Yue verfasserin aut Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic> 2018transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids. Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids. Yang, Huiping oth Torres, Leticia oth Tiersch, Terrence R. oth Enthalten in Elsevier Brown, Kerry A. ELSEVIER Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar 2013 CBP : an internat. journal Tarrytown, NY (DE-627)ELV022192689 volume:218 year:2018 pages:35-45 extent:11 https://doi.org/10.1016/j.cbpa.2018.01.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_26 GBV_ILN_40 GBV_ILN_70 GBV_ILN_120 GBV_ILN_130 GBV_ILN_640 GBV_ILN_2001 GBV_ILN_2002 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2315 GBV_ILN_2568 46.00 Tiermedizin: Allgemeines VZ AR 218 2018 35-45 11
spelling	10.1016/j.cbpa.2018.01.006 doi GBV00000000000529.pica (DE-627)ELV042103622 (ELSEVIER)S1095-6433(18)30011-4 DE-627 ger DE-627 rakwb eng 570 VZ 540 VZ 610 VZ 630 VZ 22 ssgn 46.00 bkl Liu, Yue verfasserin aut Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic> 2018transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids. Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids. Yang, Huiping oth Torres, Leticia oth Tiersch, Terrence R. oth Enthalten in Elsevier Brown, Kerry A. ELSEVIER Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar 2013 CBP : an internat. journal Tarrytown, NY (DE-627)ELV022192689 volume:218 year:2018 pages:35-45 extent:11 https://doi.org/10.1016/j.cbpa.2018.01.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_26 GBV_ILN_40 GBV_ILN_70 GBV_ILN_120 GBV_ILN_130 GBV_ILN_640 GBV_ILN_2001 GBV_ILN_2002 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2315 GBV_ILN_2568 46.00 Tiermedizin: Allgemeines VZ AR 218 2018 35-45 11
allfields_unstemmed	10.1016/j.cbpa.2018.01.006 doi GBV00000000000529.pica (DE-627)ELV042103622 (ELSEVIER)S1095-6433(18)30011-4 DE-627 ger DE-627 rakwb eng 570 VZ 540 VZ 610 VZ 630 VZ 22 ssgn 46.00 bkl Liu, Yue verfasserin aut Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic> 2018transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids. Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids. Yang, Huiping oth Torres, Leticia oth Tiersch, Terrence R. oth Enthalten in Elsevier Brown, Kerry A. ELSEVIER Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar 2013 CBP : an internat. journal Tarrytown, NY (DE-627)ELV022192689 volume:218 year:2018 pages:35-45 extent:11 https://doi.org/10.1016/j.cbpa.2018.01.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_26 GBV_ILN_40 GBV_ILN_70 GBV_ILN_120 GBV_ILN_130 GBV_ILN_640 GBV_ILN_2001 GBV_ILN_2002 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2315 GBV_ILN_2568 46.00 Tiermedizin: Allgemeines VZ AR 218 2018 35-45 11
allfieldsGer	10.1016/j.cbpa.2018.01.006 doi GBV00000000000529.pica (DE-627)ELV042103622 (ELSEVIER)S1095-6433(18)30011-4 DE-627 ger DE-627 rakwb eng 570 VZ 540 VZ 610 VZ 630 VZ 22 ssgn 46.00 bkl Liu, Yue verfasserin aut Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic> 2018transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids. Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids. Yang, Huiping oth Torres, Leticia oth Tiersch, Terrence R. oth Enthalten in Elsevier Brown, Kerry A. ELSEVIER Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar 2013 CBP : an internat. journal Tarrytown, NY (DE-627)ELV022192689 volume:218 year:2018 pages:35-45 extent:11 https://doi.org/10.1016/j.cbpa.2018.01.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_26 GBV_ILN_40 GBV_ILN_70 GBV_ILN_120 GBV_ILN_130 GBV_ILN_640 GBV_ILN_2001 GBV_ILN_2002 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2315 GBV_ILN_2568 46.00 Tiermedizin: Allgemeines VZ AR 218 2018 35-45 11
allfieldsSound	10.1016/j.cbpa.2018.01.006 doi GBV00000000000529.pica (DE-627)ELV042103622 (ELSEVIER)S1095-6433(18)30011-4 DE-627 ger DE-627 rakwb eng 570 VZ 540 VZ 610 VZ 630 VZ 22 ssgn 46.00 bkl Liu, Yue verfasserin aut Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic> 2018transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids. Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids. Yang, Huiping oth Torres, Leticia oth Tiersch, Terrence R. oth Enthalten in Elsevier Brown, Kerry A. ELSEVIER Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar 2013 CBP : an internat. journal Tarrytown, NY (DE-627)ELV022192689 volume:218 year:2018 pages:35-45 extent:11 https://doi.org/10.1016/j.cbpa.2018.01.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_26 GBV_ILN_40 GBV_ILN_70 GBV_ILN_120 GBV_ILN_130 GBV_ILN_640 GBV_ILN_2001 GBV_ILN_2002 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2315 GBV_ILN_2568 46.00 Tiermedizin: Allgemeines VZ AR 218 2018 35-45 11
language	English
source	Enthalten in Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar Tarrytown, NY volume:218 year:2018 pages:35-45 extent:11
sourceStr	Enthalten in Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar Tarrytown, NY volume:218 year:2018 pages:35-45 extent:11
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container_title	Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar
authorswithroles_txt_mv	Liu, Yue @@aut@@ Yang, Huiping @@oth@@ Torres, Leticia @@oth@@ Tiersch, Terrence R. @@oth@@
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author	Liu, Yue
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topic_title	570 VZ 540 VZ 610 VZ 630 VZ 22 ssgn 46.00 bkl Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic>
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hierarchy_parent_title	Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar
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dewey-tens	570 - Life sciences; biology 540 - Chemistry 610 - Medicine & health 630 - Agriculture
hierarchy_top_title	Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar
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title	Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic>
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title_full	Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic>
author_sort	Liu, Yue
journal	Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar
journalStr	Use of provisioning ecosystem services drives loss of functional traits across land use intensification gradients in tropical forests in Madagascar
lang_code	eng
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container_start_page	35
author_browse	Liu, Yue
container_volume	218
physical	11
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format_se	Elektronische Aufsätze
author-letter	Liu, Yue
doi_str_mv	10.1016/j.cbpa.2018.01.006
dewey-full	570 540 610 630
title_sort	activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>xenotoca eiseni</ce:italic>
title_auth	Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic>
abstract	Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids.
abstractGer	Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids.
abstract_unstemmed	Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4 °C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81–516 mOsmol/kg. The highest motility (19 ± 6%) was at 305 mOsmol/kg and swim remained for 84 h. Glucose (300–700 mOsmol/kg), NaCl (50–600 mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5–160 mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5–160 mM dissociated spermatozeugmata within 10 min. Solutions of NaCl-NaOH at pH 11.6 to 12.4 dissociated spermatozeugmata within 1 min. The percentage of viable cells had no significant differences (P = 0.2033) among different concentrations of CaCl2, but it was lower (P < 0.0001) at pH 12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids.
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title_short	Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic>
url	https://doi.org/10.1016/j.cbpa.2018.01.006
remote_bool	true
author2	Yang, Huiping Torres, Leticia Tiersch, Terrence R.
author2Str	Yang, Huiping Torres, Leticia Tiersch, Terrence R.
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doi_str	10.1016/j.cbpa.2018.01.006
up_date	2024-07-06T21:54:55.381Z
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Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, <ce:italic>Xenotoca eiseni</ce:italic>

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