Evaluation of a novel optical assay for rapid detection of methicillin-resistant Staphylococcus aureus in liquid culture
Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KI...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Knaack, Dennis [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018transfer abstract |
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| Umfang: |
3 |
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| Übergeordnetes Werk: |
Enthalten in: HIV-1 group O infection in Cameroon from 2006 to 2013: Prevalence, genetic diversity, evolution and public health challenges - Villabona-Arenas, Christian Julian ELSEVIER, 2015transfer abstract, New York, NY |
|---|---|
| Übergeordnetes Werk: |
volume:146 ; year:2018 ; pages:68-70 ; extent:3 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.mimet.2018.01.016 |
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| 520 | |a Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. | ||
| 520 | |a Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. | ||
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10.1016/j.mimet.2018.01.016 doi GBV00000000000150A.pica (DE-627)ELV042123054 (ELSEVIER)S0167-7012(18)30049-6 DE-627 ger DE-627 rakwb eng 570 570 DE-600 570 VZ 540 570 VZ BIODIV DE-30 fid 35.80 bkl 58.30 bkl Knaack, Dennis verfasserin aut Evaluation of a novel optical assay for rapid detection of methicillin-resistant Staphylococcus aureus in liquid culture 2018transfer abstract 3 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. Idelevich, Evgeny A. oth Körber-Irrgang, Barbara oth Kresken, Michael oth Becker, Karsten oth Enthalten in Elsevier Villabona-Arenas, Christian Julian ELSEVIER HIV-1 group O infection in Cameroon from 2006 to 2013: Prevalence, genetic diversity, evolution and public health challenges 2015transfer abstract New York, NY (DE-627)ELV018341047 volume:146 year:2018 pages:68-70 extent:3 https://doi.org/10.1016/j.mimet.2018.01.016 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 35.80 Makromolekulare Chemie VZ 58.30 Biotechnologie VZ AR 146 2018 68-70 3 045F 570 |
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10.1016/j.mimet.2018.01.016 doi GBV00000000000150A.pica (DE-627)ELV042123054 (ELSEVIER)S0167-7012(18)30049-6 DE-627 ger DE-627 rakwb eng 570 570 DE-600 570 VZ 540 570 VZ BIODIV DE-30 fid 35.80 bkl 58.30 bkl Knaack, Dennis verfasserin aut Evaluation of a novel optical assay for rapid detection of methicillin-resistant Staphylococcus aureus in liquid culture 2018transfer abstract 3 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. Idelevich, Evgeny A. oth Körber-Irrgang, Barbara oth Kresken, Michael oth Becker, Karsten oth Enthalten in Elsevier Villabona-Arenas, Christian Julian ELSEVIER HIV-1 group O infection in Cameroon from 2006 to 2013: Prevalence, genetic diversity, evolution and public health challenges 2015transfer abstract New York, NY (DE-627)ELV018341047 volume:146 year:2018 pages:68-70 extent:3 https://doi.org/10.1016/j.mimet.2018.01.016 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 35.80 Makromolekulare Chemie VZ 58.30 Biotechnologie VZ AR 146 2018 68-70 3 045F 570 |
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10.1016/j.mimet.2018.01.016 doi GBV00000000000150A.pica (DE-627)ELV042123054 (ELSEVIER)S0167-7012(18)30049-6 DE-627 ger DE-627 rakwb eng 570 570 DE-600 570 VZ 540 570 VZ BIODIV DE-30 fid 35.80 bkl 58.30 bkl Knaack, Dennis verfasserin aut Evaluation of a novel optical assay for rapid detection of methicillin-resistant Staphylococcus aureus in liquid culture 2018transfer abstract 3 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. Idelevich, Evgeny A. oth Körber-Irrgang, Barbara oth Kresken, Michael oth Becker, Karsten oth Enthalten in Elsevier Villabona-Arenas, Christian Julian ELSEVIER HIV-1 group O infection in Cameroon from 2006 to 2013: Prevalence, genetic diversity, evolution and public health challenges 2015transfer abstract New York, NY (DE-627)ELV018341047 volume:146 year:2018 pages:68-70 extent:3 https://doi.org/10.1016/j.mimet.2018.01.016 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 35.80 Makromolekulare Chemie VZ 58.30 Biotechnologie VZ AR 146 2018 68-70 3 045F 570 |
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10.1016/j.mimet.2018.01.016 doi GBV00000000000150A.pica (DE-627)ELV042123054 (ELSEVIER)S0167-7012(18)30049-6 DE-627 ger DE-627 rakwb eng 570 570 DE-600 570 VZ 540 570 VZ BIODIV DE-30 fid 35.80 bkl 58.30 bkl Knaack, Dennis verfasserin aut Evaluation of a novel optical assay for rapid detection of methicillin-resistant Staphylococcus aureus in liquid culture 2018transfer abstract 3 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. Idelevich, Evgeny A. oth Körber-Irrgang, Barbara oth Kresken, Michael oth Becker, Karsten oth Enthalten in Elsevier Villabona-Arenas, Christian Julian ELSEVIER HIV-1 group O infection in Cameroon from 2006 to 2013: Prevalence, genetic diversity, evolution and public health challenges 2015transfer abstract New York, NY (DE-627)ELV018341047 volume:146 year:2018 pages:68-70 extent:3 https://doi.org/10.1016/j.mimet.2018.01.016 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 35.80 Makromolekulare Chemie VZ 58.30 Biotechnologie VZ AR 146 2018 68-70 3 045F 570 |
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10.1016/j.mimet.2018.01.016 doi GBV00000000000150A.pica (DE-627)ELV042123054 (ELSEVIER)S0167-7012(18)30049-6 DE-627 ger DE-627 rakwb eng 570 570 DE-600 570 VZ 540 570 VZ BIODIV DE-30 fid 35.80 bkl 58.30 bkl Knaack, Dennis verfasserin aut Evaluation of a novel optical assay for rapid detection of methicillin-resistant Staphylococcus aureus in liquid culture 2018transfer abstract 3 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. Idelevich, Evgeny A. oth Körber-Irrgang, Barbara oth Kresken, Michael oth Becker, Karsten oth Enthalten in Elsevier Villabona-Arenas, Christian Julian ELSEVIER HIV-1 group O infection in Cameroon from 2006 to 2013: Prevalence, genetic diversity, evolution and public health challenges 2015transfer abstract New York, NY (DE-627)ELV018341047 volume:146 year:2018 pages:68-70 extent:3 https://doi.org/10.1016/j.mimet.2018.01.016 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 35.80 Makromolekulare Chemie VZ 58.30 Biotechnologie VZ AR 146 2018 68-70 3 045F 570 |
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Enthalten in HIV-1 group O infection in Cameroon from 2006 to 2013: Prevalence, genetic diversity, evolution and public health challenges New York, NY volume:146 year:2018 pages:68-70 extent:3 |
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HIV-1 group O infection in Cameroon from 2006 to 2013: Prevalence, genetic diversity, evolution and public health challenges |
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evaluation of a novel optical assay for rapid detection of methicillin-resistant staphylococcus aureus in liquid culture |
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Evaluation of a novel optical assay for rapid detection of methicillin-resistant Staphylococcus aureus in liquid culture |
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Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. |
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Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. |
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Valid detection of methicillin-resistant Staphylococcus aureus (MRSA) is a precondition for MRSA prevention measures. Most of the available MRSA screening tests are based either on the identification of colony material grown on solid agar media or on direct molecular approaches. The HB&L MRSA KIT (Alifax, Polverara, Italy) is designed to screen for MRSA in liquid cultures applying the light scattering technology. Here, the assay was evaluated with pure culture isolates from solid agar media on a representative subset of 29 MRSA and 171 methicillin-susceptible S. aureus clinical strains from a German multicenter study reflecting the ratio of MRSA to MSSA in Germany. Additionally, 53 mecA/mecC-MRSA comprising hospital-, community and livestock-associated MRSA as well as 20 clinical coagulase-negative staphylococci (CoNS) challenge strains were tested. Including the results of the assay-immanent confirmatory tests for positively tested strains, the sensitivity, specificity and the positive and negative predictive values were 100%. The methicillin-resistance was also correctly identified for all challenge strains. |
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