Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae)

Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effec...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Fiaz, Muhammad [verfasserIn] Martínez, Luis Carlos Costa, Marilza da Silva Cossolin, Jamile Fernanda Silva Plata-Rueda, Angelica Gonçalves, Wagner Gonzaga Sant’Ana, Antônio Euzébio Goulart Zanuncio, José Cola Serrão, José Eduardo

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018transfer abstract

Schlagwörter:	Bioinsecticide Ultrastructure Respirometer rate Acetogenin Autophagy Velvet bean caterpillar

Umfang:	8

Übergeordnetes Werk:	Enthalten in: Erysipelas, the “Other” Cellulitis: A Practical Guide for Nurse Practitioners - Fetters, Lisa ELSEVIER, 2021, EES : official journal of the International Society of Ecotoxicology and Environmental safety, Amsterdam
Übergeordnetes Werk:	volume:156 ; year:2018 ; day:30 ; month:07 ; pages:1-8 ; extent:8

Links:	Volltext

DOI / URN:	10.1016/j.ecoenv.2018.02.080

Katalog-ID:	ELV042571235

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520			\|a Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis.
520			\|a Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis.
650		7	\|a Bioinsecticide \|2 Elsevier
650		7	\|a Ultrastructure \|2 Elsevier
650		7	\|a Respirometer rate \|2 Elsevier
650		7	\|a Acetogenin \|2 Elsevier
650		7	\|a Autophagy \|2 Elsevier
650		7	\|a Velvet bean caterpillar \|2 Elsevier
700	1		\|a Martínez, Luis Carlos \|4 oth
700	1		\|a Costa, Marilza da Silva \|4 oth
700	1		\|a Cossolin, Jamile Fernanda Silva \|4 oth
700	1		\|a Plata-Rueda, Angelica \|4 oth
700	1		\|a Gonçalves, Wagner Gonzaga \|4 oth
700	1		\|a Sant’Ana, Antônio Euzébio Goulart \|4 oth
700	1		\|a Zanuncio, José Cola \|4 oth
700	1		\|a Serrão, José Eduardo \|4 oth
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author_variant	m f mf
matchkey_str	fiazmuhammadmartnezluiscarloscostamarilz:2018----:qaoiidchsooiaadlrsrcuacagsnhmduclsfetlcniasaem
hierarchy_sort_str	2018transfer abstract
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publishDate	2018
allfields	10.1016/j.ecoenv.2018.02.080 doi GBV00000000000492.pica (DE-627)ELV042571235 (ELSEVIER)S0147-6513(18)30179-9 DE-627 ger DE-627 rakwb eng 610 VZ 44.63 bkl Fiaz, Muhammad verfasserin aut Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae) 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis. Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis. Bioinsecticide Elsevier Ultrastructure Elsevier Respirometer rate Elsevier Acetogenin Elsevier Autophagy Elsevier Velvet bean caterpillar Elsevier Martínez, Luis Carlos oth Costa, Marilza da Silva oth Cossolin, Jamile Fernanda Silva oth Plata-Rueda, Angelica oth Gonçalves, Wagner Gonzaga oth Sant’Ana, Antônio Euzébio Goulart oth Zanuncio, José Cola oth Serrão, José Eduardo oth Enthalten in Elsevier Fetters, Lisa ELSEVIER Erysipelas, the “Other” Cellulitis: A Practical Guide for Nurse Practitioners 2021 EES : official journal of the International Society of Ecotoxicology and Environmental safety Amsterdam (DE-627)ELV006765629 volume:156 year:2018 day:30 month:07 pages:1-8 extent:8 https://doi.org/10.1016/j.ecoenv.2018.02.080 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 44.63 Krankenpflege VZ AR 156 2018 30 0730 1-8 8
spelling	10.1016/j.ecoenv.2018.02.080 doi GBV00000000000492.pica (DE-627)ELV042571235 (ELSEVIER)S0147-6513(18)30179-9 DE-627 ger DE-627 rakwb eng 610 VZ 44.63 bkl Fiaz, Muhammad verfasserin aut Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae) 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis. Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis. Bioinsecticide Elsevier Ultrastructure Elsevier Respirometer rate Elsevier Acetogenin Elsevier Autophagy Elsevier Velvet bean caterpillar Elsevier Martínez, Luis Carlos oth Costa, Marilza da Silva oth Cossolin, Jamile Fernanda Silva oth Plata-Rueda, Angelica oth Gonçalves, Wagner Gonzaga oth Sant’Ana, Antônio Euzébio Goulart oth Zanuncio, José Cola oth Serrão, José Eduardo oth Enthalten in Elsevier Fetters, Lisa ELSEVIER Erysipelas, the “Other” Cellulitis: A Practical Guide for Nurse Practitioners 2021 EES : official journal of the International Society of Ecotoxicology and Environmental safety Amsterdam (DE-627)ELV006765629 volume:156 year:2018 day:30 month:07 pages:1-8 extent:8 https://doi.org/10.1016/j.ecoenv.2018.02.080 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 44.63 Krankenpflege VZ AR 156 2018 30 0730 1-8 8
allfields_unstemmed	10.1016/j.ecoenv.2018.02.080 doi GBV00000000000492.pica (DE-627)ELV042571235 (ELSEVIER)S0147-6513(18)30179-9 DE-627 ger DE-627 rakwb eng 610 VZ 44.63 bkl Fiaz, Muhammad verfasserin aut Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae) 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis. Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis. Bioinsecticide Elsevier Ultrastructure Elsevier Respirometer rate Elsevier Acetogenin Elsevier Autophagy Elsevier Velvet bean caterpillar Elsevier Martínez, Luis Carlos oth Costa, Marilza da Silva oth Cossolin, Jamile Fernanda Silva oth Plata-Rueda, Angelica oth Gonçalves, Wagner Gonzaga oth Sant’Ana, Antônio Euzébio Goulart oth Zanuncio, José Cola oth Serrão, José Eduardo oth Enthalten in Elsevier Fetters, Lisa ELSEVIER Erysipelas, the “Other” Cellulitis: A Practical Guide for Nurse Practitioners 2021 EES : official journal of the International Society of Ecotoxicology and Environmental safety Amsterdam (DE-627)ELV006765629 volume:156 year:2018 day:30 month:07 pages:1-8 extent:8 https://doi.org/10.1016/j.ecoenv.2018.02.080 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 44.63 Krankenpflege VZ AR 156 2018 30 0730 1-8 8
allfieldsGer	10.1016/j.ecoenv.2018.02.080 doi GBV00000000000492.pica (DE-627)ELV042571235 (ELSEVIER)S0147-6513(18)30179-9 DE-627 ger DE-627 rakwb eng 610 VZ 44.63 bkl Fiaz, Muhammad verfasserin aut Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae) 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis. Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis. Bioinsecticide Elsevier Ultrastructure Elsevier Respirometer rate Elsevier Acetogenin Elsevier Autophagy Elsevier Velvet bean caterpillar Elsevier Martínez, Luis Carlos oth Costa, Marilza da Silva oth Cossolin, Jamile Fernanda Silva oth Plata-Rueda, Angelica oth Gonçalves, Wagner Gonzaga oth Sant’Ana, Antônio Euzébio Goulart oth Zanuncio, José Cola oth Serrão, José Eduardo oth Enthalten in Elsevier Fetters, Lisa ELSEVIER Erysipelas, the “Other” Cellulitis: A Practical Guide for Nurse Practitioners 2021 EES : official journal of the International Society of Ecotoxicology and Environmental safety Amsterdam (DE-627)ELV006765629 volume:156 year:2018 day:30 month:07 pages:1-8 extent:8 https://doi.org/10.1016/j.ecoenv.2018.02.080 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 44.63 Krankenpflege VZ AR 156 2018 30 0730 1-8 8
allfieldsSound	10.1016/j.ecoenv.2018.02.080 doi GBV00000000000492.pica (DE-627)ELV042571235 (ELSEVIER)S0147-6513(18)30179-9 DE-627 ger DE-627 rakwb eng 610 VZ 44.63 bkl Fiaz, Muhammad verfasserin aut Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae) 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis. Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis. Bioinsecticide Elsevier Ultrastructure Elsevier Respirometer rate Elsevier Acetogenin Elsevier Autophagy Elsevier Velvet bean caterpillar Elsevier Martínez, Luis Carlos oth Costa, Marilza da Silva oth Cossolin, Jamile Fernanda Silva oth Plata-Rueda, Angelica oth Gonçalves, Wagner Gonzaga oth Sant’Ana, Antônio Euzébio Goulart oth Zanuncio, José Cola oth Serrão, José Eduardo oth Enthalten in Elsevier Fetters, Lisa ELSEVIER Erysipelas, the “Other” Cellulitis: A Practical Guide for Nurse Practitioners 2021 EES : official journal of the International Society of Ecotoxicology and Environmental safety Amsterdam (DE-627)ELV006765629 volume:156 year:2018 day:30 month:07 pages:1-8 extent:8 https://doi.org/10.1016/j.ecoenv.2018.02.080 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 44.63 Krankenpflege VZ AR 156 2018 30 0730 1-8 8
language	English
source	Enthalten in Erysipelas, the “Other” Cellulitis: A Practical Guide for Nurse Practitioners Amsterdam volume:156 year:2018 day:30 month:07 pages:1-8 extent:8
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topic_facet	Bioinsecticide Ultrastructure Respirometer rate Acetogenin Autophagy Velvet bean caterpillar
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container_title	Erysipelas, the “Other” Cellulitis: A Practical Guide for Nurse Practitioners
authorswithroles_txt_mv	Fiaz, Muhammad @@aut@@ Martínez, Luis Carlos @@oth@@ Costa, Marilza da Silva @@oth@@ Cossolin, Jamile Fernanda Silva @@oth@@ Plata-Rueda, Angelica @@oth@@ Gonçalves, Wagner Gonzaga @@oth@@ Sant’Ana, Antônio Euzébio Goulart @@oth@@ Zanuncio, José Cola @@oth@@ Serrão, José Eduardo @@oth@@
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author	Fiaz, Muhammad
spellingShingle	Fiaz, Muhammad ddc 610 bkl 44.63 Elsevier Bioinsecticide Elsevier Ultrastructure Elsevier Respirometer rate Elsevier Acetogenin Elsevier Autophagy Elsevier Velvet bean caterpillar Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae)
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topic_title	610 VZ 44.63 bkl Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae) Bioinsecticide Elsevier Ultrastructure Elsevier Respirometer rate Elsevier Acetogenin Elsevier Autophagy Elsevier Velvet bean caterpillar Elsevier
topic	ddc 610 bkl 44.63 Elsevier Bioinsecticide Elsevier Ultrastructure Elsevier Respirometer rate Elsevier Acetogenin Elsevier Autophagy Elsevier Velvet bean caterpillar
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title	Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae)
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title_full	Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae)
author_sort	Fiaz, Muhammad
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title_sort	squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>anticarsia gemmatalis</ce:italic> (lepidoptera: noctuidae)
title_auth	Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae)
abstract	Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis.
abstractGer	Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis.
abstract_unstemmed	Annonaceous acetogenins (Annona squamosa Linnaeus) comprises of a series of natural products which are extracted from Annonaceae species, squamocin proved to be highly efficient among those agents. Squamocin is mostly referred as a lethal agent for midgut cells of different insects, with toxic effects when tested against larva of some insects. In present study, LC50 and LC90 of squamocin for A. gemmatalis Hübner (Lepidoptera: Noctuidae) were calculated using probit analysis. Morphological changes in midgut cells were analyzed under light, fluorescence and transmission electron microscopes when larvae were treated with LC50 and LC90 of squamocin for 24, 48 and 72 h. Results revealed that the maximum damage to midgut cells was found under LC90 where it showed digestive cells with enlarged basal labyrinth, highly vacuolated cytoplasm, damaged apical surface, cell protrusions to the gut lumen, autophagy and cell death. The midgut goblet cells showed a strong disorganization of their microvilli. Likewise, in insects treated with squamocin, mitochondria were not marked with Mitotracker fluorescent probe, suggesting some molecular damage in these organelles, which was reinforced by decrease in the respiration rate in these insects. These results demonstrate that squamocin has potential to induce enough morphological changes in midgut through epithelial cell damage in A. gemmatalis.
collection_details	GBV_USEFLAG_U GBV_ELV SYSFLAG_U
title_short	Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae)
url	https://doi.org/10.1016/j.ecoenv.2018.02.080
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author2Str	Martínez, Luis Carlos Costa, Marilza da Silva Cossolin, Jamile Fernanda Silva Plata-Rueda, Angelica Gonçalves, Wagner Gonzaga Sant’Ana, Antônio Euzébio Goulart Zanuncio, José Cola Serrão, José Eduardo
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up_date	2024-07-06T23:09:10.089Z
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Squamocin induce histological and ultrastructural changes in the midgut cells of <ce:italic>Anticarsia gemmatalis</ce:italic> (Lepidoptera: Noctuidae)

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