Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk
A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was perf...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Du, Meihong [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2018transfer abstract |
|---|
| Schlagwörter: |
|---|
| Umfang: |
8 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma - Tanaka, Hajime ELSEVIER, 2022, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:91 ; year:2018 ; pages:92-99 ; extent:8 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.foodcont.2018.03.032 |
|---|
| Katalog-ID: |
ELV043129048 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | ELV043129048 | ||
| 003 | DE-627 | ||
| 005 | 20230626003159.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180726s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.foodcont.2018.03.032 |2 doi | |
| 028 | 5 | 2 | |a GBV00000000000237A.pica |
| 035 | |a (DE-627)ELV043129048 | ||
| 035 | |a (ELSEVIER)S0956-7135(18)30139-7 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | |a 630 |a 640 | |
| 082 | 0 | 4 | |a 630 |q DE-600 |
| 082 | 0 | 4 | |a 640 |q DE-600 |
| 082 | 0 | 4 | |a 610 |q VZ |
| 100 | 1 | |a Du, Meihong |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk |
| 264 | 1 | |c 2018transfer abstract | |
| 300 | |a 8 | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. | ||
| 520 | |a A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. | ||
| 650 | 7 | |a Salmonella |2 Elsevier | |
| 650 | 7 | |a Pre-treatment |2 Elsevier | |
| 650 | 7 | |a Immune-magnetic separation (IMS) |2 Elsevier | |
| 650 | 7 | |a Rapid detection |2 Elsevier | |
| 650 | 7 | |a Immune-magnetic beads (IMBs) |2 Elsevier | |
| 700 | 1 | |a Li, Jingwen |4 oth | |
| 700 | 1 | |a Zhao, Ruixue |4 oth | |
| 700 | 1 | |a Yang, Yin |4 oth | |
| 700 | 1 | |a Wang, Yanfei |4 oth | |
| 700 | 1 | |a Ma, Kai |4 oth | |
| 700 | 1 | |a Cheng, Xiaoyan |4 oth | |
| 700 | 1 | |a Wan, Yuping |4 oth | |
| 700 | 1 | |a Wu, Xiaosheng |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Tanaka, Hajime ELSEVIER |t Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma |d 2022 |g Amsterdam [u.a.] |w (DE-627)ELV009139680 |
| 773 | 1 | 8 | |g volume:91 |g year:2018 |g pages:92-99 |g extent:8 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.foodcont.2018.03.032 |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a GBV_ELV | ||
| 912 | |a SYSFLAG_U | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |d 91 |j 2018 |h 92-99 |g 8 | ||
| 953 | |2 045F |a 630 | ||
| author_variant |
m d md |
|---|---|
| matchkey_str |
dumeihonglijingwenzhaoruixueyangyinwangy:2018----:fetvperamntcnqeaeoimnmgeisprtofraidtcinf |
| hierarchy_sort_str |
2018transfer abstract |
| publishDate |
2018 |
| allfields |
10.1016/j.foodcont.2018.03.032 doi GBV00000000000237A.pica (DE-627)ELV043129048 (ELSEVIER)S0956-7135(18)30139-7 DE-627 ger DE-627 rakwb eng 630 640 630 DE-600 640 DE-600 610 VZ Du, Meihong verfasserin aut Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. Salmonella Elsevier Pre-treatment Elsevier Immune-magnetic separation (IMS) Elsevier Rapid detection Elsevier Immune-magnetic beads (IMBs) Elsevier Li, Jingwen oth Zhao, Ruixue oth Yang, Yin oth Wang, Yanfei oth Ma, Kai oth Cheng, Xiaoyan oth Wan, Yuping oth Wu, Xiaosheng oth Enthalten in Elsevier Science Tanaka, Hajime ELSEVIER Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma 2022 Amsterdam [u.a.] (DE-627)ELV009139680 volume:91 year:2018 pages:92-99 extent:8 https://doi.org/10.1016/j.foodcont.2018.03.032 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 91 2018 92-99 8 045F 630 |
| spelling |
10.1016/j.foodcont.2018.03.032 doi GBV00000000000237A.pica (DE-627)ELV043129048 (ELSEVIER)S0956-7135(18)30139-7 DE-627 ger DE-627 rakwb eng 630 640 630 DE-600 640 DE-600 610 VZ Du, Meihong verfasserin aut Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. Salmonella Elsevier Pre-treatment Elsevier Immune-magnetic separation (IMS) Elsevier Rapid detection Elsevier Immune-magnetic beads (IMBs) Elsevier Li, Jingwen oth Zhao, Ruixue oth Yang, Yin oth Wang, Yanfei oth Ma, Kai oth Cheng, Xiaoyan oth Wan, Yuping oth Wu, Xiaosheng oth Enthalten in Elsevier Science Tanaka, Hajime ELSEVIER Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma 2022 Amsterdam [u.a.] (DE-627)ELV009139680 volume:91 year:2018 pages:92-99 extent:8 https://doi.org/10.1016/j.foodcont.2018.03.032 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 91 2018 92-99 8 045F 630 |
| allfields_unstemmed |
10.1016/j.foodcont.2018.03.032 doi GBV00000000000237A.pica (DE-627)ELV043129048 (ELSEVIER)S0956-7135(18)30139-7 DE-627 ger DE-627 rakwb eng 630 640 630 DE-600 640 DE-600 610 VZ Du, Meihong verfasserin aut Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. Salmonella Elsevier Pre-treatment Elsevier Immune-magnetic separation (IMS) Elsevier Rapid detection Elsevier Immune-magnetic beads (IMBs) Elsevier Li, Jingwen oth Zhao, Ruixue oth Yang, Yin oth Wang, Yanfei oth Ma, Kai oth Cheng, Xiaoyan oth Wan, Yuping oth Wu, Xiaosheng oth Enthalten in Elsevier Science Tanaka, Hajime ELSEVIER Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma 2022 Amsterdam [u.a.] (DE-627)ELV009139680 volume:91 year:2018 pages:92-99 extent:8 https://doi.org/10.1016/j.foodcont.2018.03.032 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 91 2018 92-99 8 045F 630 |
| allfieldsGer |
10.1016/j.foodcont.2018.03.032 doi GBV00000000000237A.pica (DE-627)ELV043129048 (ELSEVIER)S0956-7135(18)30139-7 DE-627 ger DE-627 rakwb eng 630 640 630 DE-600 640 DE-600 610 VZ Du, Meihong verfasserin aut Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. Salmonella Elsevier Pre-treatment Elsevier Immune-magnetic separation (IMS) Elsevier Rapid detection Elsevier Immune-magnetic beads (IMBs) Elsevier Li, Jingwen oth Zhao, Ruixue oth Yang, Yin oth Wang, Yanfei oth Ma, Kai oth Cheng, Xiaoyan oth Wan, Yuping oth Wu, Xiaosheng oth Enthalten in Elsevier Science Tanaka, Hajime ELSEVIER Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma 2022 Amsterdam [u.a.] (DE-627)ELV009139680 volume:91 year:2018 pages:92-99 extent:8 https://doi.org/10.1016/j.foodcont.2018.03.032 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 91 2018 92-99 8 045F 630 |
| allfieldsSound |
10.1016/j.foodcont.2018.03.032 doi GBV00000000000237A.pica (DE-627)ELV043129048 (ELSEVIER)S0956-7135(18)30139-7 DE-627 ger DE-627 rakwb eng 630 640 630 DE-600 640 DE-600 610 VZ Du, Meihong verfasserin aut Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk 2018transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. Salmonella Elsevier Pre-treatment Elsevier Immune-magnetic separation (IMS) Elsevier Rapid detection Elsevier Immune-magnetic beads (IMBs) Elsevier Li, Jingwen oth Zhao, Ruixue oth Yang, Yin oth Wang, Yanfei oth Ma, Kai oth Cheng, Xiaoyan oth Wan, Yuping oth Wu, Xiaosheng oth Enthalten in Elsevier Science Tanaka, Hajime ELSEVIER Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma 2022 Amsterdam [u.a.] (DE-627)ELV009139680 volume:91 year:2018 pages:92-99 extent:8 https://doi.org/10.1016/j.foodcont.2018.03.032 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 91 2018 92-99 8 045F 630 |
| language |
English |
| source |
Enthalten in Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma Amsterdam [u.a.] volume:91 year:2018 pages:92-99 extent:8 |
| sourceStr |
Enthalten in Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma Amsterdam [u.a.] volume:91 year:2018 pages:92-99 extent:8 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Salmonella Pre-treatment Immune-magnetic separation (IMS) Rapid detection Immune-magnetic beads (IMBs) |
| dewey-raw |
630 |
| isfreeaccess_bool |
false |
| container_title |
Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma |
| authorswithroles_txt_mv |
Du, Meihong @@aut@@ Li, Jingwen @@oth@@ Zhao, Ruixue @@oth@@ Yang, Yin @@oth@@ Wang, Yanfei @@oth@@ Ma, Kai @@oth@@ Cheng, Xiaoyan @@oth@@ Wan, Yuping @@oth@@ Wu, Xiaosheng @@oth@@ |
| publishDateDaySort_date |
2018-01-01T00:00:00Z |
| hierarchy_top_id |
ELV009139680 |
| dewey-sort |
3630 |
| id |
ELV043129048 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV043129048</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230626003159.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180726s2018 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.foodcont.2018.03.032</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBV00000000000237A.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV043129048</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0956-7135(18)30139-7</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">630</subfield><subfield code="a">640</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">630</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">640</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Du, Meihong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2018transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">8</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Salmonella</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Pre-treatment</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Immune-magnetic separation (IMS)</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Rapid detection</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Immune-magnetic beads (IMBs)</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Jingwen</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhao, Ruixue</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Yin</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Yanfei</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ma, Kai</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cheng, Xiaoyan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wan, Yuping</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wu, Xiaosheng</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier Science</subfield><subfield code="a">Tanaka, Hajime ELSEVIER</subfield><subfield code="t">Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma</subfield><subfield code="d">2022</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV009139680</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:91</subfield><subfield code="g">year:2018</subfield><subfield code="g">pages:92-99</subfield><subfield code="g">extent:8</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.foodcont.2018.03.032</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">91</subfield><subfield code="j">2018</subfield><subfield code="h">92-99</subfield><subfield code="g">8</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">630</subfield></datafield></record></collection>
|
| author |
Du, Meihong |
| spellingShingle |
Du, Meihong ddc 630 ddc 640 ddc 610 Elsevier Salmonella Elsevier Pre-treatment Elsevier Immune-magnetic separation (IMS) Elsevier Rapid detection Elsevier Immune-magnetic beads (IMBs) Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk |
| authorStr |
Du, Meihong |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)ELV009139680 |
| format |
electronic Article |
| dewey-ones |
630 - Agriculture & related technologies 640 - Home & family management 610 - Medicine & health |
| delete_txt_mv |
keep |
| author_role |
aut |
| collection |
elsevier |
| remote_str |
true |
| illustrated |
Not Illustrated |
| topic_title |
630 640 630 DE-600 640 DE-600 610 VZ Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk Salmonella Elsevier Pre-treatment Elsevier Immune-magnetic separation (IMS) Elsevier Rapid detection Elsevier Immune-magnetic beads (IMBs) Elsevier |
| topic |
ddc 630 ddc 640 ddc 610 Elsevier Salmonella Elsevier Pre-treatment Elsevier Immune-magnetic separation (IMS) Elsevier Rapid detection Elsevier Immune-magnetic beads (IMBs) |
| topic_unstemmed |
ddc 630 ddc 640 ddc 610 Elsevier Salmonella Elsevier Pre-treatment Elsevier Immune-magnetic separation (IMS) Elsevier Rapid detection Elsevier Immune-magnetic beads (IMBs) |
| topic_browse |
ddc 630 ddc 640 ddc 610 Elsevier Salmonella Elsevier Pre-treatment Elsevier Immune-magnetic separation (IMS) Elsevier Rapid detection Elsevier Immune-magnetic beads (IMBs) |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
j l jl r z rz y y yy y w yw k m km x c xc y w yw x w xw |
| hierarchy_parent_title |
Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma |
| hierarchy_parent_id |
ELV009139680 |
| dewey-tens |
630 - Agriculture 640 - Home & family management 610 - Medicine & health |
| hierarchy_top_title |
Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)ELV009139680 |
| title |
Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk |
| ctrlnum |
(DE-627)ELV043129048 (ELSEVIER)S0956-7135(18)30139-7 |
| title_full |
Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk |
| author_sort |
Du, Meihong |
| journal |
Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma |
| journalStr |
Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
600 - Technology |
| recordtype |
marc |
| publishDateSort |
2018 |
| contenttype_str_mv |
zzz |
| container_start_page |
92 |
| author_browse |
Du, Meihong |
| container_volume |
91 |
| physical |
8 |
| class |
630 640 630 DE-600 640 DE-600 610 VZ |
| format_se |
Elektronische Aufsätze |
| author-letter |
Du, Meihong |
| doi_str_mv |
10.1016/j.foodcont.2018.03.032 |
| dewey-full |
630 640 610 |
| title_sort |
effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of salmonella in milk |
| title_auth |
Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk |
| abstract |
A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. |
| abstractGer |
A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. |
| abstract_unstemmed |
A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples. |
| collection_details |
GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA |
| title_short |
Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk |
| url |
https://doi.org/10.1016/j.foodcont.2018.03.032 |
| remote_bool |
true |
| author2 |
Li, Jingwen Zhao, Ruixue Yang, Yin Wang, Yanfei Ma, Kai Cheng, Xiaoyan Wan, Yuping Wu, Xiaosheng |
| author2Str |
Li, Jingwen Zhao, Ruixue Yang, Yin Wang, Yanfei Ma, Kai Cheng, Xiaoyan Wan, Yuping Wu, Xiaosheng |
| ppnlink |
ELV009139680 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth oth oth oth oth |
| doi_str |
10.1016/j.foodcont.2018.03.032 |
| up_date |
2024-07-06T18:00:45.757Z |
| _version_ |
1803853596729540608 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV043129048</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230626003159.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180726s2018 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.foodcont.2018.03.032</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBV00000000000237A.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV043129048</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0956-7135(18)30139-7</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">630</subfield><subfield code="a">640</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">630</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">640</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Du, Meihong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2018transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">8</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella. Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 106 CFU/mL of Salmonella. IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10−1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Salmonella</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Pre-treatment</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Immune-magnetic separation (IMS)</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Rapid detection</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Immune-magnetic beads (IMBs)</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Jingwen</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhao, Ruixue</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Yin</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Yanfei</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ma, Kai</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cheng, Xiaoyan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wan, Yuping</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wu, Xiaosheng</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier Science</subfield><subfield code="a">Tanaka, Hajime ELSEVIER</subfield><subfield code="t">Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma</subfield><subfield code="d">2022</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV009139680</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:91</subfield><subfield code="g">year:2018</subfield><subfield code="g">pages:92-99</subfield><subfield code="g">extent:8</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.foodcont.2018.03.032</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">91</subfield><subfield code="j">2018</subfield><subfield code="h">92-99</subfield><subfield code="g">8</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">630</subfield></datafield></record></collection>
|
| score |
7.399205 |