Derivation of chicken primordial germ cells using an indirect Co-culture system
Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Xie, Long [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2019transfer abstract |
|---|
| Schlagwörter: |
|---|
| Umfang: |
7 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance - Chen, Anyi ELSEVIER, 2023, an international journal of animal reproduction, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:123 ; year:2019 ; day:1 ; month:01 ; pages:83-89 ; extent:7 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.theriogenology.2018.09.017 |
|---|
| Katalog-ID: |
ELV044494947 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | ELV044494947 | ||
| 003 | DE-627 | ||
| 005 | 20230626005246.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 181113s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.theriogenology.2018.09.017 |2 doi | |
| 028 | 5 | 2 | |a GBV00000000000400.pica |
| 035 | |a (DE-627)ELV044494947 | ||
| 035 | |a (ELSEVIER)S0093-691X(18)30805-7 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 070 |a 004 |q VZ |
| 084 | |a LING |q DE-30 |2 fid | ||
| 084 | |a 54.00 |2 bkl | ||
| 084 | |a 53.71 |2 bkl | ||
| 100 | 1 | |a Xie, Long |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Derivation of chicken primordial germ cells using an indirect Co-culture system |
| 264 | 1 | |c 2019transfer abstract | |
| 300 | |a 7 | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. | ||
| 520 | |a Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. | ||
| 650 | 7 | |a Primordial germ cells |2 Elsevier | |
| 650 | 7 | |a Chicken |2 Elsevier | |
| 650 | 7 | |a In vitro culture |2 Elsevier | |
| 700 | 1 | |a Lu, Zhenping |4 oth | |
| 700 | 1 | |a Chen, Dongyang |4 oth | |
| 700 | 1 | |a Yang, Mengmeng |4 oth | |
| 700 | 1 | |a Liao, Yuying |4 oth | |
| 700 | 1 | |a Mao, Wenrong |4 oth | |
| 700 | 1 | |a Mo, Lifen |4 oth | |
| 700 | 1 | |a Sun, Juanjuan |4 oth | |
| 700 | 1 | |a Yang, Wenhao |4 oth | |
| 700 | 1 | |a Xu, Huiyan |4 oth | |
| 700 | 1 | |a Lu, Kehuan |4 oth | |
| 700 | 1 | |a Lu, Yangqing |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Chen, Anyi ELSEVIER |t Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance |d 2023 |d an international journal of animal reproduction |g Amsterdam [u.a.] |w (DE-627)ELV009476539 |
| 773 | 1 | 8 | |g volume:123 |g year:2019 |g day:1 |g month:01 |g pages:83-89 |g extent:7 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.theriogenology.2018.09.017 |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a GBV_ELV | ||
| 912 | |a SYSFLAG_U | ||
| 912 | |a FID-LING | ||
| 912 | |a SSG-OPC-BBI | ||
| 936 | b | k | |a 54.00 |j Informatik: Allgemeines |q VZ |
| 936 | b | k | |a 53.71 |j Theoretische Nachrichtentechnik |q VZ |
| 951 | |a AR | ||
| 952 | |d 123 |j 2019 |b 1 |c 0101 |h 83-89 |g 7 | ||
| author_variant |
l x lx |
|---|---|
| matchkey_str |
xielongluzhenpingchendongyangyangmengmen:2019----:eiainfhcepiodagrclssnaidr |
| hierarchy_sort_str |
2019transfer abstract |
| bklnumber |
54.00 53.71 |
| publishDate |
2019 |
| allfields |
10.1016/j.theriogenology.2018.09.017 doi GBV00000000000400.pica (DE-627)ELV044494947 (ELSEVIER)S0093-691X(18)30805-7 DE-627 ger DE-627 rakwb eng 070 004 VZ LING DE-30 fid 54.00 bkl 53.71 bkl Xie, Long verfasserin aut Derivation of chicken primordial germ cells using an indirect Co-culture system 2019transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. Primordial germ cells Elsevier Chicken Elsevier In vitro culture Elsevier Lu, Zhenping oth Chen, Dongyang oth Yang, Mengmeng oth Liao, Yuying oth Mao, Wenrong oth Mo, Lifen oth Sun, Juanjuan oth Yang, Wenhao oth Xu, Huiyan oth Lu, Kehuan oth Lu, Yangqing oth Enthalten in Elsevier Science Chen, Anyi ELSEVIER Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance 2023 an international journal of animal reproduction Amsterdam [u.a.] (DE-627)ELV009476539 volume:123 year:2019 day:1 month:01 pages:83-89 extent:7 https://doi.org/10.1016/j.theriogenology.2018.09.017 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-LING SSG-OPC-BBI 54.00 Informatik: Allgemeines VZ 53.71 Theoretische Nachrichtentechnik VZ AR 123 2019 1 0101 83-89 7 |
| spelling |
10.1016/j.theriogenology.2018.09.017 doi GBV00000000000400.pica (DE-627)ELV044494947 (ELSEVIER)S0093-691X(18)30805-7 DE-627 ger DE-627 rakwb eng 070 004 VZ LING DE-30 fid 54.00 bkl 53.71 bkl Xie, Long verfasserin aut Derivation of chicken primordial germ cells using an indirect Co-culture system 2019transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. Primordial germ cells Elsevier Chicken Elsevier In vitro culture Elsevier Lu, Zhenping oth Chen, Dongyang oth Yang, Mengmeng oth Liao, Yuying oth Mao, Wenrong oth Mo, Lifen oth Sun, Juanjuan oth Yang, Wenhao oth Xu, Huiyan oth Lu, Kehuan oth Lu, Yangqing oth Enthalten in Elsevier Science Chen, Anyi ELSEVIER Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance 2023 an international journal of animal reproduction Amsterdam [u.a.] (DE-627)ELV009476539 volume:123 year:2019 day:1 month:01 pages:83-89 extent:7 https://doi.org/10.1016/j.theriogenology.2018.09.017 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-LING SSG-OPC-BBI 54.00 Informatik: Allgemeines VZ 53.71 Theoretische Nachrichtentechnik VZ AR 123 2019 1 0101 83-89 7 |
| allfields_unstemmed |
10.1016/j.theriogenology.2018.09.017 doi GBV00000000000400.pica (DE-627)ELV044494947 (ELSEVIER)S0093-691X(18)30805-7 DE-627 ger DE-627 rakwb eng 070 004 VZ LING DE-30 fid 54.00 bkl 53.71 bkl Xie, Long verfasserin aut Derivation of chicken primordial germ cells using an indirect Co-culture system 2019transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. Primordial germ cells Elsevier Chicken Elsevier In vitro culture Elsevier Lu, Zhenping oth Chen, Dongyang oth Yang, Mengmeng oth Liao, Yuying oth Mao, Wenrong oth Mo, Lifen oth Sun, Juanjuan oth Yang, Wenhao oth Xu, Huiyan oth Lu, Kehuan oth Lu, Yangqing oth Enthalten in Elsevier Science Chen, Anyi ELSEVIER Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance 2023 an international journal of animal reproduction Amsterdam [u.a.] (DE-627)ELV009476539 volume:123 year:2019 day:1 month:01 pages:83-89 extent:7 https://doi.org/10.1016/j.theriogenology.2018.09.017 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-LING SSG-OPC-BBI 54.00 Informatik: Allgemeines VZ 53.71 Theoretische Nachrichtentechnik VZ AR 123 2019 1 0101 83-89 7 |
| allfieldsGer |
10.1016/j.theriogenology.2018.09.017 doi GBV00000000000400.pica (DE-627)ELV044494947 (ELSEVIER)S0093-691X(18)30805-7 DE-627 ger DE-627 rakwb eng 070 004 VZ LING DE-30 fid 54.00 bkl 53.71 bkl Xie, Long verfasserin aut Derivation of chicken primordial germ cells using an indirect Co-culture system 2019transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. Primordial germ cells Elsevier Chicken Elsevier In vitro culture Elsevier Lu, Zhenping oth Chen, Dongyang oth Yang, Mengmeng oth Liao, Yuying oth Mao, Wenrong oth Mo, Lifen oth Sun, Juanjuan oth Yang, Wenhao oth Xu, Huiyan oth Lu, Kehuan oth Lu, Yangqing oth Enthalten in Elsevier Science Chen, Anyi ELSEVIER Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance 2023 an international journal of animal reproduction Amsterdam [u.a.] (DE-627)ELV009476539 volume:123 year:2019 day:1 month:01 pages:83-89 extent:7 https://doi.org/10.1016/j.theriogenology.2018.09.017 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-LING SSG-OPC-BBI 54.00 Informatik: Allgemeines VZ 53.71 Theoretische Nachrichtentechnik VZ AR 123 2019 1 0101 83-89 7 |
| allfieldsSound |
10.1016/j.theriogenology.2018.09.017 doi GBV00000000000400.pica (DE-627)ELV044494947 (ELSEVIER)S0093-691X(18)30805-7 DE-627 ger DE-627 rakwb eng 070 004 VZ LING DE-30 fid 54.00 bkl 53.71 bkl Xie, Long verfasserin aut Derivation of chicken primordial germ cells using an indirect Co-culture system 2019transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. Primordial germ cells Elsevier Chicken Elsevier In vitro culture Elsevier Lu, Zhenping oth Chen, Dongyang oth Yang, Mengmeng oth Liao, Yuying oth Mao, Wenrong oth Mo, Lifen oth Sun, Juanjuan oth Yang, Wenhao oth Xu, Huiyan oth Lu, Kehuan oth Lu, Yangqing oth Enthalten in Elsevier Science Chen, Anyi ELSEVIER Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance 2023 an international journal of animal reproduction Amsterdam [u.a.] (DE-627)ELV009476539 volume:123 year:2019 day:1 month:01 pages:83-89 extent:7 https://doi.org/10.1016/j.theriogenology.2018.09.017 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-LING SSG-OPC-BBI 54.00 Informatik: Allgemeines VZ 53.71 Theoretische Nachrichtentechnik VZ AR 123 2019 1 0101 83-89 7 |
| language |
English |
| source |
Enthalten in Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance Amsterdam [u.a.] volume:123 year:2019 day:1 month:01 pages:83-89 extent:7 |
| sourceStr |
Enthalten in Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance Amsterdam [u.a.] volume:123 year:2019 day:1 month:01 pages:83-89 extent:7 |
| format_phy_str_mv |
Article |
| bklname |
Informatik: Allgemeines Theoretische Nachrichtentechnik |
| institution |
findex.gbv.de |
| topic_facet |
Primordial germ cells Chicken In vitro culture |
| dewey-raw |
070 |
| isfreeaccess_bool |
false |
| container_title |
Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance |
| authorswithroles_txt_mv |
Xie, Long @@aut@@ Lu, Zhenping @@oth@@ Chen, Dongyang @@oth@@ Yang, Mengmeng @@oth@@ Liao, Yuying @@oth@@ Mao, Wenrong @@oth@@ Mo, Lifen @@oth@@ Sun, Juanjuan @@oth@@ Yang, Wenhao @@oth@@ Xu, Huiyan @@oth@@ Lu, Kehuan @@oth@@ Lu, Yangqing @@oth@@ |
| publishDateDaySort_date |
2019-01-01T00:00:00Z |
| hierarchy_top_id |
ELV009476539 |
| dewey-sort |
270 |
| id |
ELV044494947 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV044494947</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230626005246.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">181113s2019 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.theriogenology.2018.09.017</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBV00000000000400.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV044494947</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0093-691X(18)30805-7</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">070</subfield><subfield code="a">004</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">LING</subfield><subfield code="q">DE-30</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">54.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">53.71</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Xie, Long</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Derivation of chicken primordial germ cells using an indirect Co-culture system</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Primordial germ cells</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Chicken</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">In vitro culture</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Zhenping</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chen, Dongyang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Mengmeng</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liao, Yuying</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mao, Wenrong</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mo, Lifen</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sun, Juanjuan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Wenhao</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Huiyan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Kehuan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Yangqing</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier Science</subfield><subfield code="a">Chen, Anyi ELSEVIER</subfield><subfield code="t">Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance</subfield><subfield code="d">2023</subfield><subfield code="d">an international journal of animal reproduction</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV009476539</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:123</subfield><subfield code="g">year:2019</subfield><subfield code="g">day:1</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:83-89</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.theriogenology.2018.09.017</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-LING</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-BBI</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">54.00</subfield><subfield code="j">Informatik: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">53.71</subfield><subfield code="j">Theoretische Nachrichtentechnik</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">123</subfield><subfield code="j">2019</subfield><subfield code="b">1</subfield><subfield code="c">0101</subfield><subfield code="h">83-89</subfield><subfield code="g">7</subfield></datafield></record></collection>
|
| author |
Xie, Long |
| spellingShingle |
Xie, Long ddc 070 fid LING bkl 54.00 bkl 53.71 Elsevier Primordial germ cells Elsevier Chicken Elsevier In vitro culture Derivation of chicken primordial germ cells using an indirect Co-culture system |
| authorStr |
Xie, Long |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)ELV009476539 |
| format |
electronic Article |
| dewey-ones |
070 - News media, journalism & publishing 004 - Data processing & computer science |
| delete_txt_mv |
keep |
| author_role |
aut |
| collection |
elsevier |
| remote_str |
true |
| illustrated |
Not Illustrated |
| topic_title |
070 004 VZ LING DE-30 fid 54.00 bkl 53.71 bkl Derivation of chicken primordial germ cells using an indirect Co-culture system Primordial germ cells Elsevier Chicken Elsevier In vitro culture Elsevier |
| topic |
ddc 070 fid LING bkl 54.00 bkl 53.71 Elsevier Primordial germ cells Elsevier Chicken Elsevier In vitro culture |
| topic_unstemmed |
ddc 070 fid LING bkl 54.00 bkl 53.71 Elsevier Primordial germ cells Elsevier Chicken Elsevier In vitro culture |
| topic_browse |
ddc 070 fid LING bkl 54.00 bkl 53.71 Elsevier Primordial germ cells Elsevier Chicken Elsevier In vitro culture |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
z l zl d c dc m y my y l yl w m wm l m lm j s js w y wy h x hx k l kl y l yl |
| hierarchy_parent_title |
Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance |
| hierarchy_parent_id |
ELV009476539 |
| dewey-tens |
070 - News media, journalism & publishing 000 - Computer science, knowledge & systems |
| hierarchy_top_title |
Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)ELV009476539 |
| title |
Derivation of chicken primordial germ cells using an indirect Co-culture system |
| ctrlnum |
(DE-627)ELV044494947 (ELSEVIER)S0093-691X(18)30805-7 |
| title_full |
Derivation of chicken primordial germ cells using an indirect Co-culture system |
| author_sort |
Xie, Long |
| journal |
Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance |
| journalStr |
Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
000 - Computer science, information & general works |
| recordtype |
marc |
| publishDateSort |
2019 |
| contenttype_str_mv |
zzz |
| container_start_page |
83 |
| author_browse |
Xie, Long |
| container_volume |
123 |
| physical |
7 |
| class |
070 004 VZ LING DE-30 fid 54.00 bkl 53.71 bkl |
| format_se |
Elektronische Aufsätze |
| author-letter |
Xie, Long |
| doi_str_mv |
10.1016/j.theriogenology.2018.09.017 |
| dewey-full |
070 004 |
| title_sort |
derivation of chicken primordial germ cells using an indirect co-culture system |
| title_auth |
Derivation of chicken primordial germ cells using an indirect Co-culture system |
| abstract |
Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. |
| abstractGer |
Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. |
| abstract_unstemmed |
Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. |
| collection_details |
GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-LING SSG-OPC-BBI |
| title_short |
Derivation of chicken primordial germ cells using an indirect Co-culture system |
| url |
https://doi.org/10.1016/j.theriogenology.2018.09.017 |
| remote_bool |
true |
| author2 |
Lu, Zhenping Chen, Dongyang Yang, Mengmeng Liao, Yuying Mao, Wenrong Mo, Lifen Sun, Juanjuan Yang, Wenhao Xu, Huiyan Lu, Kehuan Lu, Yangqing |
| author2Str |
Lu, Zhenping Chen, Dongyang Yang, Mengmeng Liao, Yuying Mao, Wenrong Mo, Lifen Sun, Juanjuan Yang, Wenhao Xu, Huiyan Lu, Kehuan Lu, Yangqing |
| ppnlink |
ELV009476539 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth oth oth oth oth oth oth oth |
| doi_str |
10.1016/j.theriogenology.2018.09.017 |
| up_date |
2024-07-06T21:38:47.066Z |
| _version_ |
1803867313476206592 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV044494947</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230626005246.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">181113s2019 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.theriogenology.2018.09.017</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBV00000000000400.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV044494947</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0093-691X(18)30805-7</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">070</subfield><subfield code="a">004</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">LING</subfield><subfield code="q">DE-30</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">54.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">53.71</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Xie, Long</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Derivation of chicken primordial germ cells using an indirect Co-culture system</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Primordial germ cells</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Chicken</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">In vitro culture</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Zhenping</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chen, Dongyang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Mengmeng</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liao, Yuying</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mao, Wenrong</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mo, Lifen</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sun, Juanjuan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Wenhao</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Huiyan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Kehuan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Yangqing</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier Science</subfield><subfield code="a">Chen, Anyi ELSEVIER</subfield><subfield code="t">Multi-source monitoring information fusion method for dam health diagnosis based on Wasserstein distance</subfield><subfield code="d">2023</subfield><subfield code="d">an international journal of animal reproduction</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV009476539</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:123</subfield><subfield code="g">year:2019</subfield><subfield code="g">day:1</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:83-89</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.theriogenology.2018.09.017</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-LING</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-BBI</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">54.00</subfield><subfield code="j">Informatik: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">53.71</subfield><subfield code="j">Theoretische Nachrichtentechnik</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">123</subfield><subfield code="j">2019</subfield><subfield code="b">1</subfield><subfield code="c">0101</subfield><subfield code="h">83-89</subfield><subfield code="g">7</subfield></datafield></record></collection>
|
| score |
7.4019136 |