Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber <ce:italic>Apostichopus japonicus</ce:italic>
Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this stu...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Huo, Da [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019transfer abstract |
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| Umfang: |
17 |
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| Übergeordnetes Werk: |
Enthalten in: A second-order - Wang, Yuan-Ming ELSEVIER, 2022, New York, NY [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:193 ; year:2019 ; day:20 ; month:02 ; pages:27-43 ; extent:17 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.jprot.2018.12.020 |
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ELV045385467 |
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| 245 | 1 | 0 | |a Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber <ce:italic>Apostichopus japonicus</ce:italic> |
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| 520 | |a Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. | ||
| 520 | |a Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. | ||
| 700 | 1 | |a Sun, Lina |4 oth | |
| 700 | 1 | |a Zhang, Libin |4 oth | |
| 700 | 1 | |a Ru, Xiaoshang |4 oth | |
| 700 | 1 | |a Liu, Shilin |4 oth | |
| 700 | 1 | |a Yang, Xinyuan |4 oth | |
| 700 | 1 | |a Yang, Hongsheng |4 oth | |
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10.1016/j.jprot.2018.12.020 doi GBV00000000000483.pica (DE-627)ELV045385467 (ELSEVIER)S1874-3919(18)30449-4 DE-627 ger DE-627 rakwb eng 510 004 VZ 31.80 bkl 54.80 bkl Huo, Da verfasserin aut Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber <ce:italic>Apostichopus japonicus</ce:italic> 2019transfer abstract 17 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. Sun, Lina oth Zhang, Libin oth Ru, Xiaoshang oth Liu, Shilin oth Yang, Xinyuan oth Yang, Hongsheng oth Enthalten in Elsevier Wang, Yuan-Ming ELSEVIER A second-order 2022 New York, NY [u.a.] (DE-627)ELV008045720 volume:193 year:2019 day:20 month:02 pages:27-43 extent:17 https://doi.org/10.1016/j.jprot.2018.12.020 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-MAT 31.80 Angewandte Mathematik VZ 54.80 Angewandte Informatik VZ AR 193 2019 20 0220 27-43 17 |
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10.1016/j.jprot.2018.12.020 doi GBV00000000000483.pica (DE-627)ELV045385467 (ELSEVIER)S1874-3919(18)30449-4 DE-627 ger DE-627 rakwb eng 510 004 VZ 31.80 bkl 54.80 bkl Huo, Da verfasserin aut Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber <ce:italic>Apostichopus japonicus</ce:italic> 2019transfer abstract 17 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. Sun, Lina oth Zhang, Libin oth Ru, Xiaoshang oth Liu, Shilin oth Yang, Xinyuan oth Yang, Hongsheng oth Enthalten in Elsevier Wang, Yuan-Ming ELSEVIER A second-order 2022 New York, NY [u.a.] (DE-627)ELV008045720 volume:193 year:2019 day:20 month:02 pages:27-43 extent:17 https://doi.org/10.1016/j.jprot.2018.12.020 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-MAT 31.80 Angewandte Mathematik VZ 54.80 Angewandte Informatik VZ AR 193 2019 20 0220 27-43 17 |
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10.1016/j.jprot.2018.12.020 doi GBV00000000000483.pica (DE-627)ELV045385467 (ELSEVIER)S1874-3919(18)30449-4 DE-627 ger DE-627 rakwb eng 510 004 VZ 31.80 bkl 54.80 bkl Huo, Da verfasserin aut Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber <ce:italic>Apostichopus japonicus</ce:italic> 2019transfer abstract 17 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. Sun, Lina oth Zhang, Libin oth Ru, Xiaoshang oth Liu, Shilin oth Yang, Xinyuan oth Yang, Hongsheng oth Enthalten in Elsevier Wang, Yuan-Ming ELSEVIER A second-order 2022 New York, NY [u.a.] (DE-627)ELV008045720 volume:193 year:2019 day:20 month:02 pages:27-43 extent:17 https://doi.org/10.1016/j.jprot.2018.12.020 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-MAT 31.80 Angewandte Mathematik VZ 54.80 Angewandte Informatik VZ AR 193 2019 20 0220 27-43 17 |
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10.1016/j.jprot.2018.12.020 doi GBV00000000000483.pica (DE-627)ELV045385467 (ELSEVIER)S1874-3919(18)30449-4 DE-627 ger DE-627 rakwb eng 510 004 VZ 31.80 bkl 54.80 bkl Huo, Da verfasserin aut Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber <ce:italic>Apostichopus japonicus</ce:italic> 2019transfer abstract 17 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. Sun, Lina oth Zhang, Libin oth Ru, Xiaoshang oth Liu, Shilin oth Yang, Xinyuan oth Yang, Hongsheng oth Enthalten in Elsevier Wang, Yuan-Ming ELSEVIER A second-order 2022 New York, NY [u.a.] (DE-627)ELV008045720 volume:193 year:2019 day:20 month:02 pages:27-43 extent:17 https://doi.org/10.1016/j.jprot.2018.12.020 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-MAT 31.80 Angewandte Mathematik VZ 54.80 Angewandte Informatik VZ AR 193 2019 20 0220 27-43 17 |
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10.1016/j.jprot.2018.12.020 doi GBV00000000000483.pica (DE-627)ELV045385467 (ELSEVIER)S1874-3919(18)30449-4 DE-627 ger DE-627 rakwb eng 510 004 VZ 31.80 bkl 54.80 bkl Huo, Da verfasserin aut Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber <ce:italic>Apostichopus japonicus</ce:italic> 2019transfer abstract 17 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. Sun, Lina oth Zhang, Libin oth Ru, Xiaoshang oth Liu, Shilin oth Yang, Xinyuan oth Yang, Hongsheng oth Enthalten in Elsevier Wang, Yuan-Ming ELSEVIER A second-order 2022 New York, NY [u.a.] (DE-627)ELV008045720 volume:193 year:2019 day:20 month:02 pages:27-43 extent:17 https://doi.org/10.1016/j.jprot.2018.12.020 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-MAT 31.80 Angewandte Mathematik VZ 54.80 Angewandte Informatik VZ AR 193 2019 20 0220 27-43 17 |
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Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. 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Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. 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global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber <ce:italic>apostichopus japonicus</ce:italic> |
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Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber <ce:italic>Apostichopus japonicus</ce:italic> |
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Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. |
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Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. |
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Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed that 262, 155 and 433 proteins were differentially regulated in response to heat, hypoxia, and heat plus hypoxia, respectively. Expression of key genes and proteins were measured by real-time PCR and western blot to validate the accuracy of the proteome files. Results showed that the interaction between these two stressors has an additive effect at the proteome level. Proteins involved in amino acid and carbohydrate metabolic processes were largely induced by heat while repressed by hypoxia. Exposure to multiple climate change stressors caused several proteins involved in lipid metabolic progress to be down-regulated and lipid catabolic processes were induced. Additionally, hypoxia and the combined stress induced proteins involved in iron homeostasis. ATP synthesis and gluconeogenesis were induced under heat and the combined stress, while ATP and glycogen synthesis were depressed under hypoxia. Proteins related with immune and defense response were largely induced and protein synthesis ability was depressed under all three stresses. Thus, sea cucumber may adopt different strategies to cope with varied environmental stress, and the situation in heat group is more similar with the combined treatment than hypoxia group. These proteomic changes in response to high-temperature and low-oxygen levels may provide insights into the defense strategies of sea cucumber in response to global climate changes. |
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