Two novel MKKs (MKK4 and MKK7) from <ce:italic>Ctenopharyngodon idella</ce:italic> are involved in the intestinal immune response to bacterial muramyl dipeptide challenge
Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Qu, Fufa [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019transfer abstract |
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| Umfang: |
12 |
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| Übergeordnetes Werk: |
Enthalten in: GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis - Wearne, Travis A. ELSEVIER, 2016transfer abstract, ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:93 ; year:2019 ; pages:103-114 ; extent:12 |
| Links: |
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| DOI / URN: |
10.1016/j.dci.2019.01.001 |
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ELV04544319X |
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| 245 | 1 | 0 | |a Two novel MKKs (MKK4 and MKK7) from <ce:italic>Ctenopharyngodon idella</ce:italic> are involved in the intestinal immune response to bacterial muramyl dipeptide challenge |
| 264 | 1 | |c 2019transfer abstract | |
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| 520 | |a Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. | ||
| 520 | |a Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. | ||
| 650 | 7 | |a Expression profile |2 Elsevier | |
| 650 | 7 | |a Muramyl dipeptide |2 Elsevier | |
| 650 | 7 | |a Grass carp |2 Elsevier | |
| 650 | 7 | |a MKK4 and MKK7 |2 Elsevier | |
| 650 | 7 | |a Intestinal inflammation |2 Elsevier | |
| 700 | 1 | |a Tang, Jianzhou |4 oth | |
| 700 | 1 | |a Peng, Xiangyu |4 oth | |
| 700 | 1 | |a Zhang, Hui |4 oth | |
| 700 | 1 | |a Shi, Liping |4 oth | |
| 700 | 1 | |a Huang, Zhenzhen |4 oth | |
| 700 | 1 | |a Xu, Wenqian |4 oth | |
| 700 | 1 | |a Chen, Huiqing |4 oth | |
| 700 | 1 | |a Shen, Ying |4 oth | |
| 700 | 1 | |a Yan, Jinpeng |4 oth | |
| 700 | 1 | |a Li, Jianzhong |4 oth | |
| 700 | 1 | |a Lu, Shuangqing |4 oth | |
| 700 | 1 | |a Liu, Zhen |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Wearne, Travis A. ELSEVIER |t GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis |d 2016transfer abstract |d ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology |g Amsterdam [u.a.] |w (DE-627)ELV019920881 |
| 773 | 1 | 8 | |g volume:93 |g year:2019 |g pages:103-114 |g extent:12 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.dci.2019.01.001 |3 Volltext |
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| 936 | b | k | |a 44.88 |j Urologie |j Nephrologie |q VZ |
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| 952 | |d 93 |j 2019 |h 103-114 |g 12 | ||
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2019 |
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10.1016/j.dci.2019.01.001 doi GBV00000000000488.pica (DE-627)ELV04544319X (ELSEVIER)S0145-305X(18)30584-6 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Qu, Fufa verfasserin aut Two novel MKKs (MKK4 and MKK7) from <ce:italic>Ctenopharyngodon idella</ce:italic> are involved in the intestinal immune response to bacterial muramyl dipeptide challenge 2019transfer abstract 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. Expression profile Elsevier Muramyl dipeptide Elsevier Grass carp Elsevier MKK4 and MKK7 Elsevier Intestinal inflammation Elsevier Tang, Jianzhou oth Peng, Xiangyu oth Zhang, Hui oth Shi, Liping oth Huang, Zhenzhen oth Xu, Wenqian oth Chen, Huiqing oth Shen, Ying oth Yan, Jinpeng oth Li, Jianzhong oth Lu, Shuangqing oth Liu, Zhen oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:93 year:2019 pages:103-114 extent:12 https://doi.org/10.1016/j.dci.2019.01.001 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 93 2019 103-114 12 |
| spelling |
10.1016/j.dci.2019.01.001 doi GBV00000000000488.pica (DE-627)ELV04544319X (ELSEVIER)S0145-305X(18)30584-6 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Qu, Fufa verfasserin aut Two novel MKKs (MKK4 and MKK7) from <ce:italic>Ctenopharyngodon idella</ce:italic> are involved in the intestinal immune response to bacterial muramyl dipeptide challenge 2019transfer abstract 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. Expression profile Elsevier Muramyl dipeptide Elsevier Grass carp Elsevier MKK4 and MKK7 Elsevier Intestinal inflammation Elsevier Tang, Jianzhou oth Peng, Xiangyu oth Zhang, Hui oth Shi, Liping oth Huang, Zhenzhen oth Xu, Wenqian oth Chen, Huiqing oth Shen, Ying oth Yan, Jinpeng oth Li, Jianzhong oth Lu, Shuangqing oth Liu, Zhen oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:93 year:2019 pages:103-114 extent:12 https://doi.org/10.1016/j.dci.2019.01.001 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 93 2019 103-114 12 |
| allfields_unstemmed |
10.1016/j.dci.2019.01.001 doi GBV00000000000488.pica (DE-627)ELV04544319X (ELSEVIER)S0145-305X(18)30584-6 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Qu, Fufa verfasserin aut Two novel MKKs (MKK4 and MKK7) from <ce:italic>Ctenopharyngodon idella</ce:italic> are involved in the intestinal immune response to bacterial muramyl dipeptide challenge 2019transfer abstract 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. Expression profile Elsevier Muramyl dipeptide Elsevier Grass carp Elsevier MKK4 and MKK7 Elsevier Intestinal inflammation Elsevier Tang, Jianzhou oth Peng, Xiangyu oth Zhang, Hui oth Shi, Liping oth Huang, Zhenzhen oth Xu, Wenqian oth Chen, Huiqing oth Shen, Ying oth Yan, Jinpeng oth Li, Jianzhong oth Lu, Shuangqing oth Liu, Zhen oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:93 year:2019 pages:103-114 extent:12 https://doi.org/10.1016/j.dci.2019.01.001 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 93 2019 103-114 12 |
| allfieldsGer |
10.1016/j.dci.2019.01.001 doi GBV00000000000488.pica (DE-627)ELV04544319X (ELSEVIER)S0145-305X(18)30584-6 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Qu, Fufa verfasserin aut Two novel MKKs (MKK4 and MKK7) from <ce:italic>Ctenopharyngodon idella</ce:italic> are involved in the intestinal immune response to bacterial muramyl dipeptide challenge 2019transfer abstract 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. Expression profile Elsevier Muramyl dipeptide Elsevier Grass carp Elsevier MKK4 and MKK7 Elsevier Intestinal inflammation Elsevier Tang, Jianzhou oth Peng, Xiangyu oth Zhang, Hui oth Shi, Liping oth Huang, Zhenzhen oth Xu, Wenqian oth Chen, Huiqing oth Shen, Ying oth Yan, Jinpeng oth Li, Jianzhong oth Lu, Shuangqing oth Liu, Zhen oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:93 year:2019 pages:103-114 extent:12 https://doi.org/10.1016/j.dci.2019.01.001 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 93 2019 103-114 12 |
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10.1016/j.dci.2019.01.001 doi GBV00000000000488.pica (DE-627)ELV04544319X (ELSEVIER)S0145-305X(18)30584-6 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Qu, Fufa verfasserin aut Two novel MKKs (MKK4 and MKK7) from <ce:italic>Ctenopharyngodon idella</ce:italic> are involved in the intestinal immune response to bacterial muramyl dipeptide challenge 2019transfer abstract 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. Expression profile Elsevier Muramyl dipeptide Elsevier Grass carp Elsevier MKK4 and MKK7 Elsevier Intestinal inflammation Elsevier Tang, Jianzhou oth Peng, Xiangyu oth Zhang, Hui oth Shi, Liping oth Huang, Zhenzhen oth Xu, Wenqian oth Chen, Huiqing oth Shen, Ying oth Yan, Jinpeng oth Li, Jianzhong oth Lu, Shuangqing oth Liu, Zhen oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:93 year:2019 pages:103-114 extent:12 https://doi.org/10.1016/j.dci.2019.01.001 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 93 2019 103-114 12 |
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Enthalten in GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis Amsterdam [u.a.] volume:93 year:2019 pages:103-114 extent:12 |
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Enthalten in GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis Amsterdam [u.a.] volume:93 year:2019 pages:103-114 extent:12 |
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GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis |
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two novel mkks (mkk4 and mkk7) from <ce:italic>ctenopharyngodon idella</ce:italic> are involved in the intestinal immune response to bacterial muramyl dipeptide challenge |
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Two novel MKKs (MKK4 and MKK7) from <ce:italic>Ctenopharyngodon idella</ce:italic> are involved in the intestinal immune response to bacterial muramyl dipeptide challenge |
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Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. |
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Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. |
| abstract_unstemmed |
Mitogen-activated protein kinase kinases (MKKs) are a class of evolutionarily conserved signalling intermediates of the MAPK signalling pathway that can be activated by a diverse range of pathogenic stimuli and are crucial for the regulation of host immune defence. In this study, two fish MKK genes (CiMKK4 and CiMKK7) were first identified and characterized from grass carp (Ctenopharyngodon idella). Similar to other reported MKKs, the present CiMKK4 and CiMKK7 contained a conserved serine/threonine protein kinase (S_TKc) domain and a canonical dual phosphorylation motif. Quantitative real-time PCR results showed that CiMKK4 and CiMKK7 were broadly transcribed in all selected tissues and developmental stages of grass carp. The mRNA expression levels of CiMKK4 and CiMKK7 in the intestine were significantly induced by bacterial muramyl dipeptide (MDP) challenge in a time-dependent manner (P < 0.01). Additionally, the stimulatory effects of bacterial MDP on CiMKK4 and CiMKK7 expression in the intestine were inhibited by the bioactive dipeptide β-alanyl-l-histidine (carnosine) and alanyl-glutamine (Ala-Gln) (P < 0.05). Moreover, overexpression analysis revealed that CiMKK4 and CiMKK7 were localized throughout the entire cell and could significantly enhance AP-1 reporter gene activation in HEK293T cells. Taken together, these results provide the first experimental demonstration that CiMKK4 and CiMKK7 are involved in the intestinal immune response to MDP challenge in C. idella, which may provide new insight into the bacterial-induced intestinal inflammation of bony fishes. |
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| title_short |
Two novel MKKs (MKK4 and MKK7) from <ce:italic>Ctenopharyngodon idella</ce:italic> are involved in the intestinal immune response to bacterial muramyl dipeptide challenge |
| url |
https://doi.org/10.1016/j.dci.2019.01.001 |
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Tang, Jianzhou Peng, Xiangyu Zhang, Hui Shi, Liping Huang, Zhenzhen Xu, Wenqian Chen, Huiqing Shen, Ying Yan, Jinpeng Li, Jianzhong Lu, Shuangqing Liu, Zhen |
| author2Str |
Tang, Jianzhou Peng, Xiangyu Zhang, Hui Shi, Liping Huang, Zhenzhen Xu, Wenqian Chen, Huiqing Shen, Ying Yan, Jinpeng Li, Jianzhong Lu, Shuangqing Liu, Zhen |
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| doi_str |
10.1016/j.dci.2019.01.001 |
| up_date |
2024-07-06T17:33:08.586Z |
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