Evaluation of in vivo expressed <ce:italic>Borrelia burgdorferi</ce:italic> antigens for improved IgM serodiagnosis of early Lyme disease
Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sens...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Brandt, Kevin S. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019transfer abstract |
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| Umfang: |
7 |
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| Übergeordnetes Werk: |
Enthalten in: Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications - Rodrigues, Jéssica S. ELSEVIER, 2023, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:93 ; year:2019 ; number:3 ; pages:196-202 ; extent:7 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.diagmicrobio.2018.09.012 |
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| 520 | |a Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. | ||
| 520 | |a Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. | ||
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| 700 | 1 | |a Biggerstaff, Brad J. |4 oth | |
| 700 | 1 | |a Gilmore, Robert D. |4 oth | |
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10.1016/j.diagmicrobio.2018.09.012 doi GBV00000000000515.pica (DE-627)ELV045734313 (ELSEVIER)S0732-8893(18)30435-8 DE-627 ger DE-627 rakwb eng 540 570 VZ BIODIV DE-30 fid 35.80 bkl 58.30 bkl Brandt, Kevin S. verfasserin aut Evaluation of in vivo expressed <ce:italic>Borrelia burgdorferi</ce:italic> antigens for improved IgM serodiagnosis of early Lyme disease 2019transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. Ullmann, Amy J. oth Molins, Claudia R. oth Horiuchi, Kalanthe oth Biggerstaff, Brad J. oth Gilmore, Robert D. oth Enthalten in Elsevier Science Rodrigues, Jéssica S. ELSEVIER Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications 2023 Amsterdam [u.a.] (DE-627)ELV009877355 volume:93 year:2019 number:3 pages:196-202 extent:7 https://doi.org/10.1016/j.diagmicrobio.2018.09.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 35.80 Makromolekulare Chemie VZ 58.30 Biotechnologie VZ AR 93 2019 3 196-202 7 |
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10.1016/j.diagmicrobio.2018.09.012 doi GBV00000000000515.pica (DE-627)ELV045734313 (ELSEVIER)S0732-8893(18)30435-8 DE-627 ger DE-627 rakwb eng 540 570 VZ BIODIV DE-30 fid 35.80 bkl 58.30 bkl Brandt, Kevin S. verfasserin aut Evaluation of in vivo expressed <ce:italic>Borrelia burgdorferi</ce:italic> antigens for improved IgM serodiagnosis of early Lyme disease 2019transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. Ullmann, Amy J. oth Molins, Claudia R. oth Horiuchi, Kalanthe oth Biggerstaff, Brad J. oth Gilmore, Robert D. oth Enthalten in Elsevier Science Rodrigues, Jéssica S. ELSEVIER Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications 2023 Amsterdam [u.a.] (DE-627)ELV009877355 volume:93 year:2019 number:3 pages:196-202 extent:7 https://doi.org/10.1016/j.diagmicrobio.2018.09.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 35.80 Makromolekulare Chemie VZ 58.30 Biotechnologie VZ AR 93 2019 3 196-202 7 |
| allfields_unstemmed |
10.1016/j.diagmicrobio.2018.09.012 doi GBV00000000000515.pica (DE-627)ELV045734313 (ELSEVIER)S0732-8893(18)30435-8 DE-627 ger DE-627 rakwb eng 540 570 VZ BIODIV DE-30 fid 35.80 bkl 58.30 bkl Brandt, Kevin S. verfasserin aut Evaluation of in vivo expressed <ce:italic>Borrelia burgdorferi</ce:italic> antigens for improved IgM serodiagnosis of early Lyme disease 2019transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. Ullmann, Amy J. oth Molins, Claudia R. oth Horiuchi, Kalanthe oth Biggerstaff, Brad J. oth Gilmore, Robert D. oth Enthalten in Elsevier Science Rodrigues, Jéssica S. ELSEVIER Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications 2023 Amsterdam [u.a.] (DE-627)ELV009877355 volume:93 year:2019 number:3 pages:196-202 extent:7 https://doi.org/10.1016/j.diagmicrobio.2018.09.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 35.80 Makromolekulare Chemie VZ 58.30 Biotechnologie VZ AR 93 2019 3 196-202 7 |
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10.1016/j.diagmicrobio.2018.09.012 doi GBV00000000000515.pica (DE-627)ELV045734313 (ELSEVIER)S0732-8893(18)30435-8 DE-627 ger DE-627 rakwb eng 540 570 VZ BIODIV DE-30 fid 35.80 bkl 58.30 bkl Brandt, Kevin S. verfasserin aut Evaluation of in vivo expressed <ce:italic>Borrelia burgdorferi</ce:italic> antigens for improved IgM serodiagnosis of early Lyme disease 2019transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. Ullmann, Amy J. oth Molins, Claudia R. oth Horiuchi, Kalanthe oth Biggerstaff, Brad J. oth Gilmore, Robert D. oth Enthalten in Elsevier Science Rodrigues, Jéssica S. ELSEVIER Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications 2023 Amsterdam [u.a.] (DE-627)ELV009877355 volume:93 year:2019 number:3 pages:196-202 extent:7 https://doi.org/10.1016/j.diagmicrobio.2018.09.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 35.80 Makromolekulare Chemie VZ 58.30 Biotechnologie VZ AR 93 2019 3 196-202 7 |
| allfieldsSound |
10.1016/j.diagmicrobio.2018.09.012 doi GBV00000000000515.pica (DE-627)ELV045734313 (ELSEVIER)S0732-8893(18)30435-8 DE-627 ger DE-627 rakwb eng 540 570 VZ BIODIV DE-30 fid 35.80 bkl 58.30 bkl Brandt, Kevin S. verfasserin aut Evaluation of in vivo expressed <ce:italic>Borrelia burgdorferi</ce:italic> antigens for improved IgM serodiagnosis of early Lyme disease 2019transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. Ullmann, Amy J. oth Molins, Claudia R. oth Horiuchi, Kalanthe oth Biggerstaff, Brad J. oth Gilmore, Robert D. oth Enthalten in Elsevier Science Rodrigues, Jéssica S. ELSEVIER Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications 2023 Amsterdam [u.a.] (DE-627)ELV009877355 volume:93 year:2019 number:3 pages:196-202 extent:7 https://doi.org/10.1016/j.diagmicrobio.2018.09.012 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA 35.80 Makromolekulare Chemie VZ 58.30 Biotechnologie VZ AR 93 2019 3 196-202 7 |
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Enthalten in Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications Amsterdam [u.a.] volume:93 year:2019 number:3 pages:196-202 extent:7 |
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Enthalten in Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications Amsterdam [u.a.] volume:93 year:2019 number:3 pages:196-202 extent:7 |
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Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications |
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Brandt, Kevin S. @@aut@@ Ullmann, Amy J. @@oth@@ Molins, Claudia R. @@oth@@ Horiuchi, Kalanthe @@oth@@ Biggerstaff, Brad J. @@oth@@ Gilmore, Robert D. @@oth@@ |
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Evaluation of in vivo expressed <ce:italic>Borrelia burgdorferi</ce:italic> antigens for improved IgM serodiagnosis of early Lyme disease |
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Evaluation of in vivo expressed <ce:italic>Borrelia burgdorferi</ce:italic> antigens for improved IgM serodiagnosis of early Lyme disease |
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Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications |
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evaluation of in vivo expressed <ce:italic>borrelia burgdorferi</ce:italic> antigens for improved igm serodiagnosis of early lyme disease |
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Evaluation of in vivo expressed <ce:italic>Borrelia burgdorferi</ce:italic> antigens for improved IgM serodiagnosis of early Lyme disease |
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Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. |
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Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. |
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Improved serologic tests are needed for accurate diagnosis and proper treatment of early stage Lyme disease. We evaluated the 3 antigens currently used for 2-tiered IgM immunoblot testing (FlaB, OspC, and BmpA) in combination with 3 additional antigens (BBA65, BBA70, and BBA73) and measured the sensitivity and specificity against a serum repository of positive and negative controls. Using 3 statistical methods for positivity cutoff determinations and scoring criteria, we found increased sensitivities for early Lyme disease when 2 of 6 antigens were positive as compared with the 2 of 3 antigen IgM criteria currently used for second-tier immunoblot scoring. Specificities for negative controls were comparable or superior to using 2 of 3 antigens. These results indicate that IgM sensitivity and specificity of serological testing for Lyme disease in the early stages of illness can be improved by employing antigens that target the initial host antibody responses. |
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Evaluation of in vivo expressed <ce:italic>Borrelia burgdorferi</ce:italic> antigens for improved IgM serodiagnosis of early Lyme disease |
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