The two TRIM25 isoforms were differentially induced in <ce:italic>Larimichthys crocea</ce:italic> post poly (I:C) stimulation
In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The lo...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zhou, Zhenzhen [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019transfer abstract |
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| Schlagwörter: |
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| Umfang: |
8 |
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| Übergeordnetes Werk: |
Enthalten in: Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention - Istanbuly, Sedralmontaha ELSEVIER, 2021, London |
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| Übergeordnetes Werk: |
volume:86 ; year:2019 ; pages:672-679 ; extent:8 |
| Links: |
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| DOI / URN: |
10.1016/j.fsi.2018.12.009 |
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ELV045759987 |
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| 245 | 1 | 4 | |a The two TRIM25 isoforms were differentially induced in <ce:italic>Larimichthys crocea</ce:italic> post poly (I:C) stimulation |
| 264 | 1 | |c 2019transfer abstract | |
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| 520 | |a In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. | ||
| 520 | |a In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. | ||
| 650 | 7 | |a Differentially expression |2 Elsevier | |
| 650 | 7 | |a poly(I:C) |2 Elsevier | |
| 650 | 7 | |a TRIM25 |2 Elsevier | |
| 650 | 7 | |a Innate immunity |2 Elsevier | |
| 650 | 7 | |a <ce:italic>Larimichthys crocea</ce:italic> |2 Elsevier | |
| 700 | 1 | |a Wei, Ke |4 oth | |
| 700 | 1 | |a Zhang, Jianshe |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Academic Press |a Istanbuly, Sedralmontaha ELSEVIER |t Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention |d 2021 |g London |w (DE-627)ELV006540406 |
| 773 | 1 | 8 | |g volume:86 |g year:2019 |g pages:672-679 |g extent:8 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.fsi.2018.12.009 |3 Volltext |
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10.1016/j.fsi.2018.12.009 doi GBV00000000000518.pica (DE-627)ELV045759987 (ELSEVIER)S1050-4648(18)30808-8 DE-627 ger DE-627 rakwb eng 610 VZ 44.85 bkl Zhou, Zhenzhen verfasserin aut The two TRIM25 isoforms were differentially induced in <ce:italic>Larimichthys crocea</ce:italic> post poly (I:C) stimulation 2019transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. Differentially expression Elsevier poly(I:C) Elsevier TRIM25 Elsevier Innate immunity Elsevier <ce:italic>Larimichthys crocea</ce:italic> Elsevier Wei, Ke oth Zhang, Jianshe oth Enthalten in Academic Press Istanbuly, Sedralmontaha ELSEVIER Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention 2021 London (DE-627)ELV006540406 volume:86 year:2019 pages:672-679 extent:8 https://doi.org/10.1016/j.fsi.2018.12.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.85 Kardiologie Angiologie VZ AR 86 2019 672-679 8 |
| spelling |
10.1016/j.fsi.2018.12.009 doi GBV00000000000518.pica (DE-627)ELV045759987 (ELSEVIER)S1050-4648(18)30808-8 DE-627 ger DE-627 rakwb eng 610 VZ 44.85 bkl Zhou, Zhenzhen verfasserin aut The two TRIM25 isoforms were differentially induced in <ce:italic>Larimichthys crocea</ce:italic> post poly (I:C) stimulation 2019transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. Differentially expression Elsevier poly(I:C) Elsevier TRIM25 Elsevier Innate immunity Elsevier <ce:italic>Larimichthys crocea</ce:italic> Elsevier Wei, Ke oth Zhang, Jianshe oth Enthalten in Academic Press Istanbuly, Sedralmontaha ELSEVIER Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention 2021 London (DE-627)ELV006540406 volume:86 year:2019 pages:672-679 extent:8 https://doi.org/10.1016/j.fsi.2018.12.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.85 Kardiologie Angiologie VZ AR 86 2019 672-679 8 |
| allfields_unstemmed |
10.1016/j.fsi.2018.12.009 doi GBV00000000000518.pica (DE-627)ELV045759987 (ELSEVIER)S1050-4648(18)30808-8 DE-627 ger DE-627 rakwb eng 610 VZ 44.85 bkl Zhou, Zhenzhen verfasserin aut The two TRIM25 isoforms were differentially induced in <ce:italic>Larimichthys crocea</ce:italic> post poly (I:C) stimulation 2019transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. Differentially expression Elsevier poly(I:C) Elsevier TRIM25 Elsevier Innate immunity Elsevier <ce:italic>Larimichthys crocea</ce:italic> Elsevier Wei, Ke oth Zhang, Jianshe oth Enthalten in Academic Press Istanbuly, Sedralmontaha ELSEVIER Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention 2021 London (DE-627)ELV006540406 volume:86 year:2019 pages:672-679 extent:8 https://doi.org/10.1016/j.fsi.2018.12.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.85 Kardiologie Angiologie VZ AR 86 2019 672-679 8 |
| allfieldsGer |
10.1016/j.fsi.2018.12.009 doi GBV00000000000518.pica (DE-627)ELV045759987 (ELSEVIER)S1050-4648(18)30808-8 DE-627 ger DE-627 rakwb eng 610 VZ 44.85 bkl Zhou, Zhenzhen verfasserin aut The two TRIM25 isoforms were differentially induced in <ce:italic>Larimichthys crocea</ce:italic> post poly (I:C) stimulation 2019transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. Differentially expression Elsevier poly(I:C) Elsevier TRIM25 Elsevier Innate immunity Elsevier <ce:italic>Larimichthys crocea</ce:italic> Elsevier Wei, Ke oth Zhang, Jianshe oth Enthalten in Academic Press Istanbuly, Sedralmontaha ELSEVIER Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention 2021 London (DE-627)ELV006540406 volume:86 year:2019 pages:672-679 extent:8 https://doi.org/10.1016/j.fsi.2018.12.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.85 Kardiologie Angiologie VZ AR 86 2019 672-679 8 |
| allfieldsSound |
10.1016/j.fsi.2018.12.009 doi GBV00000000000518.pica (DE-627)ELV045759987 (ELSEVIER)S1050-4648(18)30808-8 DE-627 ger DE-627 rakwb eng 610 VZ 44.85 bkl Zhou, Zhenzhen verfasserin aut The two TRIM25 isoforms were differentially induced in <ce:italic>Larimichthys crocea</ce:italic> post poly (I:C) stimulation 2019transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. Differentially expression Elsevier poly(I:C) Elsevier TRIM25 Elsevier Innate immunity Elsevier <ce:italic>Larimichthys crocea</ce:italic> Elsevier Wei, Ke oth Zhang, Jianshe oth Enthalten in Academic Press Istanbuly, Sedralmontaha ELSEVIER Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention 2021 London (DE-627)ELV006540406 volume:86 year:2019 pages:672-679 extent:8 https://doi.org/10.1016/j.fsi.2018.12.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.85 Kardiologie Angiologie VZ AR 86 2019 672-679 8 |
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two trim25 isoforms were differentially induced in <ce:italic>larimichthys crocea</ce:italic> post poly (i:c) stimulation |
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The two TRIM25 isoforms were differentially induced in <ce:italic>Larimichthys crocea</ce:italic> post poly (I:C) stimulation |
| abstract |
In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. |
| abstractGer |
In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. |
| abstract_unstemmed |
In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues. The two LcTRIM25 isoforms contained a conserved Really Interesting New Gene (RING) domain, a B-box2 domain, a Coiled-coil domain (CCD), and variable C-terminal PRY/SPRY domains. Phylogenetic analysis showed that the two LcTRIM25 isoforms of the large yellow croaker was clustered together with their counterparts from other teleost fish. The Real-time PCR analysis showed that the LcTRIM25-L and LcTRIM25-S isoforms were both ubiquitously expressed in nine examined tissues in the large yellow croaker, with predominant expressions in the liver. The expression levels of the two isoforms of LcTRIM25 were rapidly and significantly upregulated in vivo after poly (I:C) stimulation in peripheral blood, head kidney, spleen and liver. Moreover, LcTRIM25-L and LcTRIM25-S showed differential expression post poly(I:C) stimulation. LcTRIM25 may have a dual role in innate immunity via alternative gene splicing. These results indicated that LcTRIM25 is likely to be involved in antiviral immune responses. |
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