Multiple pharmacognostic characterization on hemp commercial cultivars: Focus on inflorescence water extract activity
One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ferrante, Claudio [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019transfer abstract |
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| Schlagwörter: |
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| Umfang: |
10 |
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| Übergeordnetes Werk: |
Enthalten in: Numerical simulations on static Vertical Axis Wind Turbine blade icing - Manatbayev, Rustem ELSEVIER, 2021, New York, NY [u.a.] |
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| Übergeordnetes Werk: |
volume:125 ; year:2019 ; pages:452-461 ; extent:10 |
| Links: |
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| DOI / URN: |
10.1016/j.fct.2019.01.035 |
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| 520 | |a One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. | ||
| 520 | |a One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. | ||
| 650 | 7 | |a Oxidative stress |2 Elsevier | |
| 650 | 7 | |a Hemp |2 Elsevier | |
| 650 | 7 | |a Antimicrobial activity |2 Elsevier | |
| 650 | 7 | |a Inflammation |2 Elsevier | |
| 650 | 7 | |a Ulcerative colitis |2 Elsevier | |
| 700 | 1 | |a Recinella, Lucia |4 oth | |
| 700 | 1 | |a Ronci, Maurizio |4 oth | |
| 700 | 1 | |a Menghini, Luigi |4 oth | |
| 700 | 1 | |a Brunetti, Luigi |4 oth | |
| 700 | 1 | |a Chiavaroli, Annalisa |4 oth | |
| 700 | 1 | |a Leone, Sheila |4 oth | |
| 700 | 1 | |a Di Iorio, Luigi |4 oth | |
| 700 | 1 | |a Carradori, Simone |4 oth | |
| 700 | 1 | |a Tirillini, Bruno |4 oth | |
| 700 | 1 | |a Angelini, Paola |4 oth | |
| 700 | 1 | |a Covino, Stefano |4 oth | |
| 700 | 1 | |a Venanzoni, Roberto |4 oth | |
| 700 | 1 | |a Orlando, Giustino |4 oth | |
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10.1016/j.fct.2019.01.035 doi GBV00000000000538.pica (DE-627)ELV045962510 (ELSEVIER)S0278-6915(19)30046-8 DE-627 ger DE-627 rakwb eng 530 620 VZ 52.56 bkl Ferrante, Claudio verfasserin aut Multiple pharmacognostic characterization on hemp commercial cultivars: Focus on inflorescence water extract activity 2019transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. Oxidative stress Elsevier Hemp Elsevier Antimicrobial activity Elsevier Inflammation Elsevier Ulcerative colitis Elsevier Recinella, Lucia oth Ronci, Maurizio oth Menghini, Luigi oth Brunetti, Luigi oth Chiavaroli, Annalisa oth Leone, Sheila oth Di Iorio, Luigi oth Carradori, Simone oth Tirillini, Bruno oth Angelini, Paola oth Covino, Stefano oth Venanzoni, Roberto oth Orlando, Giustino oth Enthalten in Elsevier Manatbayev, Rustem ELSEVIER Numerical simulations on static Vertical Axis Wind Turbine blade icing 2021 New York, NY [u.a.] (DE-627)ELV005709407 volume:125 year:2019 pages:452-461 extent:10 https://doi.org/10.1016/j.fct.2019.01.035 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 52.56 Regenerative Energieformen alternative Energieformen VZ AR 125 2019 452-461 10 |
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10.1016/j.fct.2019.01.035 doi GBV00000000000538.pica (DE-627)ELV045962510 (ELSEVIER)S0278-6915(19)30046-8 DE-627 ger DE-627 rakwb eng 530 620 VZ 52.56 bkl Ferrante, Claudio verfasserin aut Multiple pharmacognostic characterization on hemp commercial cultivars: Focus on inflorescence water extract activity 2019transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. Oxidative stress Elsevier Hemp Elsevier Antimicrobial activity Elsevier Inflammation Elsevier Ulcerative colitis Elsevier Recinella, Lucia oth Ronci, Maurizio oth Menghini, Luigi oth Brunetti, Luigi oth Chiavaroli, Annalisa oth Leone, Sheila oth Di Iorio, Luigi oth Carradori, Simone oth Tirillini, Bruno oth Angelini, Paola oth Covino, Stefano oth Venanzoni, Roberto oth Orlando, Giustino oth Enthalten in Elsevier Manatbayev, Rustem ELSEVIER Numerical simulations on static Vertical Axis Wind Turbine blade icing 2021 New York, NY [u.a.] (DE-627)ELV005709407 volume:125 year:2019 pages:452-461 extent:10 https://doi.org/10.1016/j.fct.2019.01.035 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 52.56 Regenerative Energieformen alternative Energieformen VZ AR 125 2019 452-461 10 |
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10.1016/j.fct.2019.01.035 doi GBV00000000000538.pica (DE-627)ELV045962510 (ELSEVIER)S0278-6915(19)30046-8 DE-627 ger DE-627 rakwb eng 530 620 VZ 52.56 bkl Ferrante, Claudio verfasserin aut Multiple pharmacognostic characterization on hemp commercial cultivars: Focus on inflorescence water extract activity 2019transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. Oxidative stress Elsevier Hemp Elsevier Antimicrobial activity Elsevier Inflammation Elsevier Ulcerative colitis Elsevier Recinella, Lucia oth Ronci, Maurizio oth Menghini, Luigi oth Brunetti, Luigi oth Chiavaroli, Annalisa oth Leone, Sheila oth Di Iorio, Luigi oth Carradori, Simone oth Tirillini, Bruno oth Angelini, Paola oth Covino, Stefano oth Venanzoni, Roberto oth Orlando, Giustino oth Enthalten in Elsevier Manatbayev, Rustem ELSEVIER Numerical simulations on static Vertical Axis Wind Turbine blade icing 2021 New York, NY [u.a.] (DE-627)ELV005709407 volume:125 year:2019 pages:452-461 extent:10 https://doi.org/10.1016/j.fct.2019.01.035 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 52.56 Regenerative Energieformen alternative Energieformen VZ AR 125 2019 452-461 10 |
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10.1016/j.fct.2019.01.035 doi GBV00000000000538.pica (DE-627)ELV045962510 (ELSEVIER)S0278-6915(19)30046-8 DE-627 ger DE-627 rakwb eng 530 620 VZ 52.56 bkl Ferrante, Claudio verfasserin aut Multiple pharmacognostic characterization on hemp commercial cultivars: Focus on inflorescence water extract activity 2019transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. Oxidative stress Elsevier Hemp Elsevier Antimicrobial activity Elsevier Inflammation Elsevier Ulcerative colitis Elsevier Recinella, Lucia oth Ronci, Maurizio oth Menghini, Luigi oth Brunetti, Luigi oth Chiavaroli, Annalisa oth Leone, Sheila oth Di Iorio, Luigi oth Carradori, Simone oth Tirillini, Bruno oth Angelini, Paola oth Covino, Stefano oth Venanzoni, Roberto oth Orlando, Giustino oth Enthalten in Elsevier Manatbayev, Rustem ELSEVIER Numerical simulations on static Vertical Axis Wind Turbine blade icing 2021 New York, NY [u.a.] (DE-627)ELV005709407 volume:125 year:2019 pages:452-461 extent:10 https://doi.org/10.1016/j.fct.2019.01.035 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 52.56 Regenerative Energieformen alternative Energieformen VZ AR 125 2019 452-461 10 |
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10.1016/j.fct.2019.01.035 doi GBV00000000000538.pica (DE-627)ELV045962510 (ELSEVIER)S0278-6915(19)30046-8 DE-627 ger DE-627 rakwb eng 530 620 VZ 52.56 bkl Ferrante, Claudio verfasserin aut Multiple pharmacognostic characterization on hemp commercial cultivars: Focus on inflorescence water extract activity 2019transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. Oxidative stress Elsevier Hemp Elsevier Antimicrobial activity Elsevier Inflammation Elsevier Ulcerative colitis Elsevier Recinella, Lucia oth Ronci, Maurizio oth Menghini, Luigi oth Brunetti, Luigi oth Chiavaroli, Annalisa oth Leone, Sheila oth Di Iorio, Luigi oth Carradori, Simone oth Tirillini, Bruno oth Angelini, Paola oth Covino, Stefano oth Venanzoni, Roberto oth Orlando, Giustino oth Enthalten in Elsevier Manatbayev, Rustem ELSEVIER Numerical simulations on static Vertical Axis Wind Turbine blade icing 2021 New York, NY [u.a.] (DE-627)ELV005709407 volume:125 year:2019 pages:452-461 extent:10 https://doi.org/10.1016/j.fct.2019.01.035 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 52.56 Regenerative Energieformen alternative Energieformen VZ AR 125 2019 452-461 10 |
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Enthalten in Numerical simulations on static Vertical Axis Wind Turbine blade icing New York, NY [u.a.] volume:125 year:2019 pages:452-461 extent:10 |
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Ferrante, Claudio @@aut@@ Recinella, Lucia @@oth@@ Ronci, Maurizio @@oth@@ Menghini, Luigi @@oth@@ Brunetti, Luigi @@oth@@ Chiavaroli, Annalisa @@oth@@ Leone, Sheila @@oth@@ Di Iorio, Luigi @@oth@@ Carradori, Simone @@oth@@ Tirillini, Bruno @@oth@@ Angelini, Paola @@oth@@ Covino, Stefano @@oth@@ Venanzoni, Roberto @@oth@@ Orlando, Giustino @@oth@@ |
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multiple pharmacognostic characterization on hemp commercial cultivars: focus on inflorescence water extract activity |
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Multiple pharmacognostic characterization on hemp commercial cultivars: Focus on inflorescence water extract activity |
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One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. |
| abstractGer |
One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. |
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One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water flower extracts from four commercial cultivars (Futura 75, Kc virtus, Carmagnola Cs and Villanova). We evaluated the phytochemical profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines, and in an ex vivo experimental model of ulcerative colitis, constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites, malondialdehyde (MDA), prostaglandin (PG)E2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on C. albicans and selected Gram positive and negative bacterial strains. |
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