Facile synthesis of IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols
In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Liu, Xueliang [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019transfer abstract |
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| Schlagwörter: |
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| Umfang: |
8 |
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| Übergeordnetes Werk: |
Enthalten in: Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications - Mohamed, S.H. ELSEVIER, 2019, the international journal of pure and applied analytical chemistry, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:203 ; year:2019 ; day:1 ; month:10 ; pages:227-234 ; extent:8 |
| Links: |
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| DOI / URN: |
10.1016/j.talanta.2019.05.070 |
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| 520 | |a In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. | ||
| 520 | |a In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. | ||
| 650 | 7 | |a IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites |2 Elsevier | |
| 650 | 7 | |a Low weight biothiols |2 Elsevier | |
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| 650 | 7 | |a Peroxidase-like activity |2 Elsevier | |
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| 700 | 1 | |a Han, Qiusen |4 oth | |
| 700 | 1 | |a Qi, Cui |4 oth | |
| 700 | 1 | |a Wang, Chen |4 oth | |
| 700 | 1 | |a Yang, Rong |4 oth | |
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10.1016/j.talanta.2019.05.070 doi GBV00000000000653.pica (DE-627)ELV047118105 (ELSEVIER)S0039-9140(19)30564-8 DE-627 ger DE-627 rakwb eng 530 620 VZ 53.56 bkl Liu, Xueliang verfasserin aut Facile synthesis of IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols 2019transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites Elsevier Low weight biothiols Elsevier Nanozyme Elsevier Peroxidase-like activity Elsevier Colorimetric detection Elsevier Wang, Xinhuan oth Han, Qiusen oth Qi, Cui oth Wang, Chen oth Yang, Rong oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:203 year:2019 day:1 month:10 pages:227-234 extent:8 https://doi.org/10.1016/j.talanta.2019.05.070 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 203 2019 1 1001 227-234 8 |
| spelling |
10.1016/j.talanta.2019.05.070 doi GBV00000000000653.pica (DE-627)ELV047118105 (ELSEVIER)S0039-9140(19)30564-8 DE-627 ger DE-627 rakwb eng 530 620 VZ 53.56 bkl Liu, Xueliang verfasserin aut Facile synthesis of IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols 2019transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites Elsevier Low weight biothiols Elsevier Nanozyme Elsevier Peroxidase-like activity Elsevier Colorimetric detection Elsevier Wang, Xinhuan oth Han, Qiusen oth Qi, Cui oth Wang, Chen oth Yang, Rong oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:203 year:2019 day:1 month:10 pages:227-234 extent:8 https://doi.org/10.1016/j.talanta.2019.05.070 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 203 2019 1 1001 227-234 8 |
| allfields_unstemmed |
10.1016/j.talanta.2019.05.070 doi GBV00000000000653.pica (DE-627)ELV047118105 (ELSEVIER)S0039-9140(19)30564-8 DE-627 ger DE-627 rakwb eng 530 620 VZ 53.56 bkl Liu, Xueliang verfasserin aut Facile synthesis of IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols 2019transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites Elsevier Low weight biothiols Elsevier Nanozyme Elsevier Peroxidase-like activity Elsevier Colorimetric detection Elsevier Wang, Xinhuan oth Han, Qiusen oth Qi, Cui oth Wang, Chen oth Yang, Rong oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:203 year:2019 day:1 month:10 pages:227-234 extent:8 https://doi.org/10.1016/j.talanta.2019.05.070 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 203 2019 1 1001 227-234 8 |
| allfieldsGer |
10.1016/j.talanta.2019.05.070 doi GBV00000000000653.pica (DE-627)ELV047118105 (ELSEVIER)S0039-9140(19)30564-8 DE-627 ger DE-627 rakwb eng 530 620 VZ 53.56 bkl Liu, Xueliang verfasserin aut Facile synthesis of IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols 2019transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites Elsevier Low weight biothiols Elsevier Nanozyme Elsevier Peroxidase-like activity Elsevier Colorimetric detection Elsevier Wang, Xinhuan oth Han, Qiusen oth Qi, Cui oth Wang, Chen oth Yang, Rong oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:203 year:2019 day:1 month:10 pages:227-234 extent:8 https://doi.org/10.1016/j.talanta.2019.05.070 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 203 2019 1 1001 227-234 8 |
| allfieldsSound |
10.1016/j.talanta.2019.05.070 doi GBV00000000000653.pica (DE-627)ELV047118105 (ELSEVIER)S0039-9140(19)30564-8 DE-627 ger DE-627 rakwb eng 530 620 VZ 53.56 bkl Liu, Xueliang verfasserin aut Facile synthesis of IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols 2019transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites Elsevier Low weight biothiols Elsevier Nanozyme Elsevier Peroxidase-like activity Elsevier Colorimetric detection Elsevier Wang, Xinhuan oth Han, Qiusen oth Qi, Cui oth Wang, Chen oth Yang, Rong oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:203 year:2019 day:1 month:10 pages:227-234 extent:8 https://doi.org/10.1016/j.talanta.2019.05.070 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 203 2019 1 1001 227-234 8 |
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Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:203 year:2019 day:1 month:10 pages:227-234 extent:8 |
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Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:203 year:2019 day:1 month:10 pages:227-234 extent:8 |
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facile synthesis of iro<ce:inf loc="post">2</ce:inf>/rgo nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols |
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Facile synthesis of IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols |
| abstract |
In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. |
| abstractGer |
In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. |
| abstract_unstemmed |
In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment. |
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Facile synthesis of IrO<ce:inf loc="post">2</ce:inf>/rGO nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols |
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