2023 - SIS-SEQ IDENTIFIES THE EARLIEST LINEAGE PRIMING REGULATORS OF DENDRITIC CELL FATE
Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor populatio...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Tomei, Sara [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
2019transfer abstract |
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| Übergeordnetes Werk: |
Enthalten in: Interfacing 2D M - Rawat, Ashima ELSEVIER, 2021, official publication of the International Society for Experimental Hematology, Amsterdam [u.a] |
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| Übergeordnetes Werk: |
volume:76 ; year:2019 ; pages:47 |
| Links: |
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| DOI / URN: |
10.1016/j.exphem.2019.06.295 |
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ELV047664177 |
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| 520 | |a Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. | ||
| 520 | |a Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. | ||
| 700 | 1 | |a Tian, Luyi |4 oth | |
| 700 | 1 | |a Schreuder, Jaring |4 oth | |
| 700 | 1 | |a Zalcenstein, Daniela |4 oth | |
| 700 | 1 | |a Tran, Jessica |4 oth | |
| 700 | 1 | |a Kocovski, Nikolce |4 oth | |
| 700 | 1 | |a Su, Shian |4 oth | |
| 700 | 1 | |a Diakumis, Peter |4 oth | |
| 700 | 1 | |a Bahlo, Melanie |4 oth | |
| 700 | 1 | |a Hodgkin, Philip |4 oth | |
| 700 | 1 | |a Ritchie, Matthew |4 oth | |
| 700 | 1 | |a Naik, Shalin |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Rawat, Ashima ELSEVIER |t Interfacing 2D M |d 2021 |d official publication of the International Society for Experimental Hematology |g Amsterdam [u.a] |w (DE-627)ELV006315852 |
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10.1016/j.exphem.2019.06.295 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000000796.pica (DE-627)ELV047664177 (ELSEVIER)S0301-472X(19)30623-X DE-627 ger DE-627 rakwb 670 530 660 VZ 33.68 bkl 35.18 bkl 52.78 bkl Tomei, Sara verfasserin aut 2023 - SIS-SEQ IDENTIFIES THE EARLIEST LINEAGE PRIMING REGULATORS OF DENDRITIC CELL FATE 2019transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. Tian, Luyi oth Schreuder, Jaring oth Zalcenstein, Daniela oth Tran, Jessica oth Kocovski, Nikolce oth Su, Shian oth Diakumis, Peter oth Bahlo, Melanie oth Hodgkin, Philip oth Ritchie, Matthew oth Naik, Shalin oth Enthalten in Elsevier Science Rawat, Ashima ELSEVIER Interfacing 2D M 2021 official publication of the International Society for Experimental Hematology Amsterdam [u.a] (DE-627)ELV006315852 volume:76 year:2019 pages:47 https://doi.org/10.1016/j.exphem.2019.06.295 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 33.68 Oberflächen Dünne Schichten Grenzflächen Physik VZ 35.18 Kolloidchemie Grenzflächenchemie VZ 52.78 Oberflächentechnik Wärmebehandlung VZ AR 76 2019 47 76.2019, S47- |
| spelling |
10.1016/j.exphem.2019.06.295 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000000796.pica (DE-627)ELV047664177 (ELSEVIER)S0301-472X(19)30623-X DE-627 ger DE-627 rakwb 670 530 660 VZ 33.68 bkl 35.18 bkl 52.78 bkl Tomei, Sara verfasserin aut 2023 - SIS-SEQ IDENTIFIES THE EARLIEST LINEAGE PRIMING REGULATORS OF DENDRITIC CELL FATE 2019transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. Tian, Luyi oth Schreuder, Jaring oth Zalcenstein, Daniela oth Tran, Jessica oth Kocovski, Nikolce oth Su, Shian oth Diakumis, Peter oth Bahlo, Melanie oth Hodgkin, Philip oth Ritchie, Matthew oth Naik, Shalin oth Enthalten in Elsevier Science Rawat, Ashima ELSEVIER Interfacing 2D M 2021 official publication of the International Society for Experimental Hematology Amsterdam [u.a] (DE-627)ELV006315852 volume:76 year:2019 pages:47 https://doi.org/10.1016/j.exphem.2019.06.295 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 33.68 Oberflächen Dünne Schichten Grenzflächen Physik VZ 35.18 Kolloidchemie Grenzflächenchemie VZ 52.78 Oberflächentechnik Wärmebehandlung VZ AR 76 2019 47 76.2019, S47- |
| allfields_unstemmed |
10.1016/j.exphem.2019.06.295 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000000796.pica (DE-627)ELV047664177 (ELSEVIER)S0301-472X(19)30623-X DE-627 ger DE-627 rakwb 670 530 660 VZ 33.68 bkl 35.18 bkl 52.78 bkl Tomei, Sara verfasserin aut 2023 - SIS-SEQ IDENTIFIES THE EARLIEST LINEAGE PRIMING REGULATORS OF DENDRITIC CELL FATE 2019transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. Tian, Luyi oth Schreuder, Jaring oth Zalcenstein, Daniela oth Tran, Jessica oth Kocovski, Nikolce oth Su, Shian oth Diakumis, Peter oth Bahlo, Melanie oth Hodgkin, Philip oth Ritchie, Matthew oth Naik, Shalin oth Enthalten in Elsevier Science Rawat, Ashima ELSEVIER Interfacing 2D M 2021 official publication of the International Society for Experimental Hematology Amsterdam [u.a] (DE-627)ELV006315852 volume:76 year:2019 pages:47 https://doi.org/10.1016/j.exphem.2019.06.295 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 33.68 Oberflächen Dünne Schichten Grenzflächen Physik VZ 35.18 Kolloidchemie Grenzflächenchemie VZ 52.78 Oberflächentechnik Wärmebehandlung VZ AR 76 2019 47 76.2019, S47- |
| allfieldsGer |
10.1016/j.exphem.2019.06.295 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000000796.pica (DE-627)ELV047664177 (ELSEVIER)S0301-472X(19)30623-X DE-627 ger DE-627 rakwb 670 530 660 VZ 33.68 bkl 35.18 bkl 52.78 bkl Tomei, Sara verfasserin aut 2023 - SIS-SEQ IDENTIFIES THE EARLIEST LINEAGE PRIMING REGULATORS OF DENDRITIC CELL FATE 2019transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. Tian, Luyi oth Schreuder, Jaring oth Zalcenstein, Daniela oth Tran, Jessica oth Kocovski, Nikolce oth Su, Shian oth Diakumis, Peter oth Bahlo, Melanie oth Hodgkin, Philip oth Ritchie, Matthew oth Naik, Shalin oth Enthalten in Elsevier Science Rawat, Ashima ELSEVIER Interfacing 2D M 2021 official publication of the International Society for Experimental Hematology Amsterdam [u.a] (DE-627)ELV006315852 volume:76 year:2019 pages:47 https://doi.org/10.1016/j.exphem.2019.06.295 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 33.68 Oberflächen Dünne Schichten Grenzflächen Physik VZ 35.18 Kolloidchemie Grenzflächenchemie VZ 52.78 Oberflächentechnik Wärmebehandlung VZ AR 76 2019 47 76.2019, S47- |
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10.1016/j.exphem.2019.06.295 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000000796.pica (DE-627)ELV047664177 (ELSEVIER)S0301-472X(19)30623-X DE-627 ger DE-627 rakwb 670 530 660 VZ 33.68 bkl 35.18 bkl 52.78 bkl Tomei, Sara verfasserin aut 2023 - SIS-SEQ IDENTIFIES THE EARLIEST LINEAGE PRIMING REGULATORS OF DENDRITIC CELL FATE 2019transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. Tian, Luyi oth Schreuder, Jaring oth Zalcenstein, Daniela oth Tran, Jessica oth Kocovski, Nikolce oth Su, Shian oth Diakumis, Peter oth Bahlo, Melanie oth Hodgkin, Philip oth Ritchie, Matthew oth Naik, Shalin oth Enthalten in Elsevier Science Rawat, Ashima ELSEVIER Interfacing 2D M 2021 official publication of the International Society for Experimental Hematology Amsterdam [u.a] (DE-627)ELV006315852 volume:76 year:2019 pages:47 https://doi.org/10.1016/j.exphem.2019.06.295 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 33.68 Oberflächen Dünne Schichten Grenzflächen Physik VZ 35.18 Kolloidchemie Grenzflächenchemie VZ 52.78 Oberflächentechnik Wärmebehandlung VZ AR 76 2019 47 76.2019, S47- |
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2023 - sis-seq identifies the earliest lineage priming regulators of dendritic cell fate |
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2023 - SIS-SEQ IDENTIFIES THE EARLIEST LINEAGE PRIMING REGULATORS OF DENDRITIC CELL FATE |
| abstract |
Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. |
| abstractGer |
Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. |
| abstract_unstemmed |
Dendritic cells (DCs) are immune cells important for the detection and immunity against pathogens, self-antigens and cancer. They include 3 subtypes, conventional DC type 1 (cDC1), cDC type 2 (cDC2) and plasmacytoid DC (pDC) which all derive from a common hematopoietic stem cell progenitor population (HSPCs). Recent evidence has shown that this population is heterogenous for fate and not all HSPCs produce every DC subtype (Naik et al., 2007). To understand the earliest lineage priming regulators of DC fate, one would have to overcome the problem of the destructive nature of scRNA-seq, which makes it impossible for a single cell to be tested for both fate and its transcription profile. Here, we achieve this with a novel approach where a single progenitor cell is allowed to divide into a few cells, some of which are tested for fate while others tested for gene expression. By correlating clonal fate with gene expression, clone-by-clone, for 109 clones, we identified 490 genes that correlated with cDC1, cDC2 and/or pDC fate bias. All genes were tested in a pooled CRISPR/Cas9 screen in DC cultures where several novel genes were identified that regulated DC fate – the knockout of some genes enhanced numbers of particular DC subtypes, whereas others reduced numbers. This system could be used to study heterogeneity of fate at clonal level in other populations, including stem cells and cancer cells to reveal the transcriptional origin of fate decisions. |
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2023 - SIS-SEQ IDENTIFIES THE EARLIEST LINEAGE PRIMING REGULATORS OF DENDRITIC CELL FATE |
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Tian, Luyi Schreuder, Jaring Zalcenstein, Daniela Tran, Jessica Kocovski, Nikolce Su, Shian Diakumis, Peter Bahlo, Melanie Hodgkin, Philip Ritchie, Matthew Naik, Shalin |
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|
| score |
7.400216 |