Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality
We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and ob...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lam, Matti [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2019transfer abstract |
|---|
| Schlagwörter: |
|---|
| Übergeordnetes Werk: |
Enthalten in: 72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS - 2012, ECR, Orlando, Fla |
|---|---|
| Übergeordnetes Werk: |
volume:383 ; year:2019 ; number:1 ; day:1 ; month:10 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.yexcr.2019.06.014 |
|---|
| Katalog-ID: |
ELV047814497 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | ELV047814497 | ||
| 003 | DE-627 | ||
| 005 | 20230626020527.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 191023s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.yexcr.2019.06.014 |2 doi | |
| 028 | 5 | 2 | |a GBV00000000000739.pica |
| 035 | |a (DE-627)ELV047814497 | ||
| 035 | |a (ELSEVIER)S0014-4827(19)30309-X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 610 |q VZ |
| 082 | 0 | 4 | |a 610 |q VZ |
| 084 | |a 44.44 |2 bkl | ||
| 100 | 1 | |a Lam, Matti |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality |
| 264 | 1 | |c 2019transfer abstract | |
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. | ||
| 520 | |a We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. | ||
| 650 | 7 | |a Neurexin |2 Elsevier | |
| 650 | 7 | |a Disease modeling |2 Elsevier | |
| 650 | 7 | |a Neural stem cell |2 Elsevier | |
| 650 | 7 | |a Induced pluripotent stem cell |2 Elsevier | |
| 650 | 7 | |a Neurexin-1 alpha |2 Elsevier | |
| 650 | 7 | |a Autism spectrum disorder |2 Elsevier | |
| 650 | 7 | |a Single cell RNA sequencing |2 Elsevier | |
| 650 | 7 | |a Neural development |2 Elsevier | |
| 700 | 1 | |a Moslem, Mohsen |4 oth | |
| 700 | 1 | |a Bryois, Julien |4 oth | |
| 700 | 1 | |a Pronk, Robin J. |4 oth | |
| 700 | 1 | |a Uhlin, Elias |4 oth | |
| 700 | 1 | |a Ellström, Ivar Dehnisch |4 oth | |
| 700 | 1 | |a Laan, Loora |4 oth | |
| 700 | 1 | |a Olive, Jessica |4 oth | |
| 700 | 1 | |a Morse, Rebecca |4 oth | |
| 700 | 1 | |a Rönnholm, Harriet |4 oth | |
| 700 | 1 | |a Louhivuori, Lauri |4 oth | |
| 700 | 1 | |a Korol, Sergiy V. |4 oth | |
| 700 | 1 | |a Dahl, Niklas |4 oth | |
| 700 | 1 | |a Uhlén, Per |4 oth | |
| 700 | 1 | |a Anderlid, Britt-Marie |4 oth | |
| 700 | 1 | |a Kele, Malin |4 oth | |
| 700 | 1 | |a Sullivan, Patrick F. |4 oth | |
| 700 | 1 | |a Falk, Anna |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Academic Press |t 72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS |d 2012 |d ECR |g Orlando, Fla |w (DE-627)ELV011050691 |
| 773 | 1 | 8 | |g volume:383 |g year:2019 |g number:1 |g day:1 |g month:10 |g pages:0 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.yexcr.2019.06.014 |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a GBV_ELV | ||
| 912 | |a SYSFLAG_U | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a GBV_ILN_70 | ||
| 936 | b | k | |a 44.44 |j Parasitologie |x Medizin |q VZ |
| 951 | |a AR | ||
| 952 | |d 383 |j 2019 |e 1 |b 1 |c 1001 |h 0 | ||
| author_variant |
m l ml |
|---|---|
| matchkey_str |
lammattimoslemmohsenbryoisjulienpronkrob:2019----:igeelnlssfuimainwtballcetlcrnapaetlceeineelseeftcocinuapo |
| hierarchy_sort_str |
2019transfer abstract |
| bklnumber |
44.44 |
| publishDate |
2019 |
| allfields |
10.1016/j.yexcr.2019.06.014 doi GBV00000000000739.pica (DE-627)ELV047814497 (ELSEVIER)S0014-4827(19)30309-X DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.44 bkl Lam, Matti verfasserin aut Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality 2019transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. Neurexin Elsevier Disease modeling Elsevier Neural stem cell Elsevier Induced pluripotent stem cell Elsevier Neurexin-1 alpha Elsevier Autism spectrum disorder Elsevier Single cell RNA sequencing Elsevier Neural development Elsevier Moslem, Mohsen oth Bryois, Julien oth Pronk, Robin J. oth Uhlin, Elias oth Ellström, Ivar Dehnisch oth Laan, Loora oth Olive, Jessica oth Morse, Rebecca oth Rönnholm, Harriet oth Louhivuori, Lauri oth Korol, Sergiy V. oth Dahl, Niklas oth Uhlén, Per oth Anderlid, Britt-Marie oth Kele, Malin oth Sullivan, Patrick F. oth Falk, Anna oth Enthalten in Academic Press 72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS 2012 ECR Orlando, Fla (DE-627)ELV011050691 volume:383 year:2019 number:1 day:1 month:10 pages:0 https://doi.org/10.1016/j.yexcr.2019.06.014 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_70 44.44 Parasitologie Medizin VZ AR 383 2019 1 1 1001 0 |
| spelling |
10.1016/j.yexcr.2019.06.014 doi GBV00000000000739.pica (DE-627)ELV047814497 (ELSEVIER)S0014-4827(19)30309-X DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.44 bkl Lam, Matti verfasserin aut Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality 2019transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. Neurexin Elsevier Disease modeling Elsevier Neural stem cell Elsevier Induced pluripotent stem cell Elsevier Neurexin-1 alpha Elsevier Autism spectrum disorder Elsevier Single cell RNA sequencing Elsevier Neural development Elsevier Moslem, Mohsen oth Bryois, Julien oth Pronk, Robin J. oth Uhlin, Elias oth Ellström, Ivar Dehnisch oth Laan, Loora oth Olive, Jessica oth Morse, Rebecca oth Rönnholm, Harriet oth Louhivuori, Lauri oth Korol, Sergiy V. oth Dahl, Niklas oth Uhlén, Per oth Anderlid, Britt-Marie oth Kele, Malin oth Sullivan, Patrick F. oth Falk, Anna oth Enthalten in Academic Press 72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS 2012 ECR Orlando, Fla (DE-627)ELV011050691 volume:383 year:2019 number:1 day:1 month:10 pages:0 https://doi.org/10.1016/j.yexcr.2019.06.014 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_70 44.44 Parasitologie Medizin VZ AR 383 2019 1 1 1001 0 |
| allfields_unstemmed |
10.1016/j.yexcr.2019.06.014 doi GBV00000000000739.pica (DE-627)ELV047814497 (ELSEVIER)S0014-4827(19)30309-X DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.44 bkl Lam, Matti verfasserin aut Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality 2019transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. Neurexin Elsevier Disease modeling Elsevier Neural stem cell Elsevier Induced pluripotent stem cell Elsevier Neurexin-1 alpha Elsevier Autism spectrum disorder Elsevier Single cell RNA sequencing Elsevier Neural development Elsevier Moslem, Mohsen oth Bryois, Julien oth Pronk, Robin J. oth Uhlin, Elias oth Ellström, Ivar Dehnisch oth Laan, Loora oth Olive, Jessica oth Morse, Rebecca oth Rönnholm, Harriet oth Louhivuori, Lauri oth Korol, Sergiy V. oth Dahl, Niklas oth Uhlén, Per oth Anderlid, Britt-Marie oth Kele, Malin oth Sullivan, Patrick F. oth Falk, Anna oth Enthalten in Academic Press 72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS 2012 ECR Orlando, Fla (DE-627)ELV011050691 volume:383 year:2019 number:1 day:1 month:10 pages:0 https://doi.org/10.1016/j.yexcr.2019.06.014 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_70 44.44 Parasitologie Medizin VZ AR 383 2019 1 1 1001 0 |
| allfieldsGer |
10.1016/j.yexcr.2019.06.014 doi GBV00000000000739.pica (DE-627)ELV047814497 (ELSEVIER)S0014-4827(19)30309-X DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.44 bkl Lam, Matti verfasserin aut Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality 2019transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. Neurexin Elsevier Disease modeling Elsevier Neural stem cell Elsevier Induced pluripotent stem cell Elsevier Neurexin-1 alpha Elsevier Autism spectrum disorder Elsevier Single cell RNA sequencing Elsevier Neural development Elsevier Moslem, Mohsen oth Bryois, Julien oth Pronk, Robin J. oth Uhlin, Elias oth Ellström, Ivar Dehnisch oth Laan, Loora oth Olive, Jessica oth Morse, Rebecca oth Rönnholm, Harriet oth Louhivuori, Lauri oth Korol, Sergiy V. oth Dahl, Niklas oth Uhlén, Per oth Anderlid, Britt-Marie oth Kele, Malin oth Sullivan, Patrick F. oth Falk, Anna oth Enthalten in Academic Press 72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS 2012 ECR Orlando, Fla (DE-627)ELV011050691 volume:383 year:2019 number:1 day:1 month:10 pages:0 https://doi.org/10.1016/j.yexcr.2019.06.014 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_70 44.44 Parasitologie Medizin VZ AR 383 2019 1 1 1001 0 |
| allfieldsSound |
10.1016/j.yexcr.2019.06.014 doi GBV00000000000739.pica (DE-627)ELV047814497 (ELSEVIER)S0014-4827(19)30309-X DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.44 bkl Lam, Matti verfasserin aut Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality 2019transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. Neurexin Elsevier Disease modeling Elsevier Neural stem cell Elsevier Induced pluripotent stem cell Elsevier Neurexin-1 alpha Elsevier Autism spectrum disorder Elsevier Single cell RNA sequencing Elsevier Neural development Elsevier Moslem, Mohsen oth Bryois, Julien oth Pronk, Robin J. oth Uhlin, Elias oth Ellström, Ivar Dehnisch oth Laan, Loora oth Olive, Jessica oth Morse, Rebecca oth Rönnholm, Harriet oth Louhivuori, Lauri oth Korol, Sergiy V. oth Dahl, Niklas oth Uhlén, Per oth Anderlid, Britt-Marie oth Kele, Malin oth Sullivan, Patrick F. oth Falk, Anna oth Enthalten in Academic Press 72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS 2012 ECR Orlando, Fla (DE-627)ELV011050691 volume:383 year:2019 number:1 day:1 month:10 pages:0 https://doi.org/10.1016/j.yexcr.2019.06.014 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_70 44.44 Parasitologie Medizin VZ AR 383 2019 1 1 1001 0 |
| language |
English |
| source |
Enthalten in 72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS Orlando, Fla volume:383 year:2019 number:1 day:1 month:10 pages:0 |
| sourceStr |
Enthalten in 72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS Orlando, Fla volume:383 year:2019 number:1 day:1 month:10 pages:0 |
| format_phy_str_mv |
Article |
| bklname |
Parasitologie |
| institution |
findex.gbv.de |
| topic_facet |
Neurexin Disease modeling Neural stem cell Induced pluripotent stem cell Neurexin-1 alpha Autism spectrum disorder Single cell RNA sequencing Neural development |
| dewey-raw |
610 |
| isfreeaccess_bool |
false |
| container_title |
72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS |
| authorswithroles_txt_mv |
Lam, Matti @@aut@@ Moslem, Mohsen @@oth@@ Bryois, Julien @@oth@@ Pronk, Robin J. @@oth@@ Uhlin, Elias @@oth@@ Ellström, Ivar Dehnisch @@oth@@ Laan, Loora @@oth@@ Olive, Jessica @@oth@@ Morse, Rebecca @@oth@@ Rönnholm, Harriet @@oth@@ Louhivuori, Lauri @@oth@@ Korol, Sergiy V. @@oth@@ Dahl, Niklas @@oth@@ Uhlén, Per @@oth@@ Anderlid, Britt-Marie @@oth@@ Kele, Malin @@oth@@ Sullivan, Patrick F. @@oth@@ Falk, Anna @@oth@@ |
| publishDateDaySort_date |
2019-01-01T00:00:00Z |
| hierarchy_top_id |
ELV011050691 |
| dewey-sort |
3610 |
| id |
ELV047814497 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV047814497</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230626020527.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">191023s2019 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.yexcr.2019.06.014</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBV00000000000739.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV047814497</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0014-4827(19)30309-X</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.44</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Lam, Matti</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019transfer abstract</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Neurexin</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Disease modeling</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Neural stem cell</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Induced pluripotent stem cell</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Neurexin-1 alpha</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Autism spectrum disorder</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Single cell RNA sequencing</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Neural development</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Moslem, Mohsen</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bryois, Julien</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pronk, Robin J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Uhlin, Elias</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ellström, Ivar Dehnisch</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Laan, Loora</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Olive, Jessica</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Morse, Rebecca</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rönnholm, Harriet</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Louhivuori, Lauri</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Korol, Sergiy V.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Dahl, Niklas</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Uhlén, Per</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Anderlid, Britt-Marie</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kele, Malin</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sullivan, Patrick F.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Falk, Anna</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Academic Press</subfield><subfield code="t">72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS</subfield><subfield code="d">2012</subfield><subfield code="d">ECR</subfield><subfield code="g">Orlando, Fla</subfield><subfield code="w">(DE-627)ELV011050691</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:383</subfield><subfield code="g">year:2019</subfield><subfield code="g">number:1</subfield><subfield code="g">day:1</subfield><subfield code="g">month:10</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.yexcr.2019.06.014</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.44</subfield><subfield code="j">Parasitologie</subfield><subfield code="x">Medizin</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">383</subfield><subfield code="j">2019</subfield><subfield code="e">1</subfield><subfield code="b">1</subfield><subfield code="c">1001</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| author |
Lam, Matti |
| spellingShingle |
Lam, Matti ddc 610 bkl 44.44 Elsevier Neurexin Elsevier Disease modeling Elsevier Neural stem cell Elsevier Induced pluripotent stem cell Elsevier Neurexin-1 alpha Elsevier Autism spectrum disorder Elsevier Single cell RNA sequencing Elsevier Neural development Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality |
| authorStr |
Lam, Matti |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)ELV011050691 |
| format |
electronic Article |
| dewey-ones |
610 - Medicine & health |
| delete_txt_mv |
keep |
| author_role |
aut |
| collection |
elsevier |
| remote_str |
true |
| illustrated |
Not Illustrated |
| topic_title |
610 VZ 44.44 bkl Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality Neurexin Elsevier Disease modeling Elsevier Neural stem cell Elsevier Induced pluripotent stem cell Elsevier Neurexin-1 alpha Elsevier Autism spectrum disorder Elsevier Single cell RNA sequencing Elsevier Neural development Elsevier |
| topic |
ddc 610 bkl 44.44 Elsevier Neurexin Elsevier Disease modeling Elsevier Neural stem cell Elsevier Induced pluripotent stem cell Elsevier Neurexin-1 alpha Elsevier Autism spectrum disorder Elsevier Single cell RNA sequencing Elsevier Neural development |
| topic_unstemmed |
ddc 610 bkl 44.44 Elsevier Neurexin Elsevier Disease modeling Elsevier Neural stem cell Elsevier Induced pluripotent stem cell Elsevier Neurexin-1 alpha Elsevier Autism spectrum disorder Elsevier Single cell RNA sequencing Elsevier Neural development |
| topic_browse |
ddc 610 bkl 44.44 Elsevier Neurexin Elsevier Disease modeling Elsevier Neural stem cell Elsevier Induced pluripotent stem cell Elsevier Neurexin-1 alpha Elsevier Autism spectrum disorder Elsevier Single cell RNA sequencing Elsevier Neural development |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
m m mm j b jb r j p rj rjp e u eu i d e id ide l l ll j o jo r m rm h r hr l l ll s v k sv svk n d nd p u pu b m a bma m k mk p f s pf pfs a f af |
| hierarchy_parent_title |
72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS |
| hierarchy_parent_id |
ELV011050691 |
| dewey-tens |
610 - Medicine & health |
| hierarchy_top_title |
72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)ELV011050691 |
| title |
Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality |
| ctrlnum |
(DE-627)ELV047814497 (ELSEVIER)S0014-4827(19)30309-X |
| title_full |
Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality |
| author_sort |
Lam, Matti |
| journal |
72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS |
| journalStr |
72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
600 - Technology |
| recordtype |
marc |
| publishDateSort |
2019 |
| contenttype_str_mv |
zzz |
| container_start_page |
0 |
| author_browse |
Lam, Matti |
| container_volume |
383 |
| class |
610 VZ 44.44 bkl |
| format_se |
Elektronische Aufsätze |
| author-letter |
Lam, Matti |
| doi_str_mv |
10.1016/j.yexcr.2019.06.014 |
| dewey-full |
610 |
| title_sort |
single cell analysis of autism patient with bi-allelic <ce:italic>nrxn1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality |
| title_auth |
Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality |
| abstract |
We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. |
| abstractGer |
We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. |
| abstract_unstemmed |
We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells. |
| collection_details |
GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_70 |
| container_issue |
1 |
| title_short |
Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality |
| url |
https://doi.org/10.1016/j.yexcr.2019.06.014 |
| remote_bool |
true |
| author2 |
Moslem, Mohsen Bryois, Julien Pronk, Robin J. Uhlin, Elias Ellström, Ivar Dehnisch Laan, Loora Olive, Jessica Morse, Rebecca Rönnholm, Harriet Louhivuori, Lauri Korol, Sergiy V. Dahl, Niklas Uhlén, Per Anderlid, Britt-Marie Kele, Malin Sullivan, Patrick F. Falk, Anna |
| author2Str |
Moslem, Mohsen Bryois, Julien Pronk, Robin J. Uhlin, Elias Ellström, Ivar Dehnisch Laan, Loora Olive, Jessica Morse, Rebecca Rönnholm, Harriet Louhivuori, Lauri Korol, Sergiy V. Dahl, Niklas Uhlén, Per Anderlid, Britt-Marie Kele, Malin Sullivan, Patrick F. Falk, Anna |
| ppnlink |
ELV011050691 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth oth oth oth oth oth oth oth oth oth oth oth oth oth |
| doi_str |
10.1016/j.yexcr.2019.06.014 |
| up_date |
2024-07-06T17:11:19.652Z |
| _version_ |
1803850486544072704 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV047814497</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230626020527.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">191023s2019 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.yexcr.2019.06.014</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBV00000000000739.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV047814497</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0014-4827(19)30309-X</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.44</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Lam, Matti</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Single cell analysis of autism patient with bi-allelic <ce:italic>NRXN1-alpha</ce:italic> deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019transfer abstract</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">We generated human iPS derived neural stem cells and differentiated cells from healthy control individuals and an individual with autism spectrum disorder carrying bi-allelic NRXN1-alpha deletion. We investigated the expression of NRXN1-alpha during neural induction and neural differentiation and observed a pivotal role for NRXN1-alpha during early neural induction and neuronal differentiation. Single cell RNA-seq pinpointed neural stem cells carrying NRXN1-alpha deletion shifting towards radial glia-like cell identity and revealed higher proportion of differentiated astroglia. Furthermore, neuronal cells carrying NRXN1-alpha deletion were identified as immature by single cell RNA-seq analysis, displayed significant depression in calcium signaling activity and presented impaired maturation action potential profile in neurons investigated with electrophysiology. Our observations propose NRXN1-alpha plays an important role for the efficient establishment of neural stem cells, in neuronal differentiation and in maturation of functional excitatory neuronal cells.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Neurexin</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Disease modeling</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Neural stem cell</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Induced pluripotent stem cell</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Neurexin-1 alpha</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Autism spectrum disorder</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Single cell RNA sequencing</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Neural development</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Moslem, Mohsen</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bryois, Julien</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pronk, Robin J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Uhlin, Elias</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ellström, Ivar Dehnisch</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Laan, Loora</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Olive, Jessica</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Morse, Rebecca</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rönnholm, Harriet</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Louhivuori, Lauri</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Korol, Sergiy V.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Dahl, Niklas</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Uhlén, Per</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Anderlid, Britt-Marie</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kele, Malin</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sullivan, Patrick F.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Falk, Anna</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Academic Press</subfield><subfield code="t">72 OUTCOMES OF COMBINATION OF HEPATITIS B IMMUNOGLOBULIN AND HEPATITIS B VACCINATION IN HIGH-RISK NEWBORNS BORN TO HBEAG-POSITIVE MOTHERS</subfield><subfield code="d">2012</subfield><subfield code="d">ECR</subfield><subfield code="g">Orlando, Fla</subfield><subfield code="w">(DE-627)ELV011050691</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:383</subfield><subfield code="g">year:2019</subfield><subfield code="g">number:1</subfield><subfield code="g">day:1</subfield><subfield code="g">month:10</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.yexcr.2019.06.014</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.44</subfield><subfield code="j">Parasitologie</subfield><subfield code="x">Medizin</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">383</subfield><subfield code="j">2019</subfield><subfield code="e">1</subfield><subfield code="b">1</subfield><subfield code="c">1001</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| score |
7.402112 |