Co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in Bacillus subtilis for alcohol detoxification
Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully co...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lu, Jing [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Numerical simulations on static Vertical Axis Wind Turbine blade icing - Manatbayev, Rustem ELSEVIER, 2021, New York, NY [u.a.] |
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| Übergeordnetes Werk: |
volume:135 ; year:2020 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.fct.2019.110890 |
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ELV048918954 |
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| 520 | |a Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. | ||
| 520 | |a Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. | ||
| 650 | 7 | |a Bacillus subtilis |2 Elsevier | |
| 650 | 7 | |a Alcohol detoxification |2 Elsevier | |
| 650 | 7 | |a Alcohol dehydrogenase |2 Elsevier | |
| 650 | 7 | |a Aldehyde dehydrogenase |2 Elsevier | |
| 650 | 7 | |a Alcohol-induced liver injury |2 Elsevier | |
| 700 | 1 | |a Zhu, Xiaoyu |4 oth | |
| 700 | 1 | |a Zhang, Chong |4 oth | |
| 700 | 1 | |a Lu, Fengxia |4 oth | |
| 700 | 1 | |a Lu, Zhaoxin |4 oth | |
| 700 | 1 | |a Lu, Yingjian |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier |a Manatbayev, Rustem ELSEVIER |t Numerical simulations on static Vertical Axis Wind Turbine blade icing |d 2021 |g New York, NY [u.a.] |w (DE-627)ELV005709407 |
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10.1016/j.fct.2019.110890 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000000887.pica (DE-627)ELV048918954 (ELSEVIER)S0278-6915(19)30680-5 DE-627 ger DE-627 rakwb eng 530 620 VZ 52.56 bkl Lu, Jing verfasserin aut Co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in Bacillus subtilis for alcohol detoxification 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. Bacillus subtilis Elsevier Alcohol detoxification Elsevier Alcohol dehydrogenase Elsevier Aldehyde dehydrogenase Elsevier Alcohol-induced liver injury Elsevier Zhu, Xiaoyu oth Zhang, Chong oth Lu, Fengxia oth Lu, Zhaoxin oth Lu, Yingjian oth Enthalten in Elsevier Manatbayev, Rustem ELSEVIER Numerical simulations on static Vertical Axis Wind Turbine blade icing 2021 New York, NY [u.a.] (DE-627)ELV005709407 volume:135 year:2020 pages:0 https://doi.org/10.1016/j.fct.2019.110890 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 52.56 Regenerative Energieformen alternative Energieformen VZ AR 135 2020 0 |
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10.1016/j.fct.2019.110890 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000000887.pica (DE-627)ELV048918954 (ELSEVIER)S0278-6915(19)30680-5 DE-627 ger DE-627 rakwb eng 530 620 VZ 52.56 bkl Lu, Jing verfasserin aut Co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in Bacillus subtilis for alcohol detoxification 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. Bacillus subtilis Elsevier Alcohol detoxification Elsevier Alcohol dehydrogenase Elsevier Aldehyde dehydrogenase Elsevier Alcohol-induced liver injury Elsevier Zhu, Xiaoyu oth Zhang, Chong oth Lu, Fengxia oth Lu, Zhaoxin oth Lu, Yingjian oth Enthalten in Elsevier Manatbayev, Rustem ELSEVIER Numerical simulations on static Vertical Axis Wind Turbine blade icing 2021 New York, NY [u.a.] (DE-627)ELV005709407 volume:135 year:2020 pages:0 https://doi.org/10.1016/j.fct.2019.110890 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 52.56 Regenerative Energieformen alternative Energieformen VZ AR 135 2020 0 |
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10.1016/j.fct.2019.110890 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000000887.pica (DE-627)ELV048918954 (ELSEVIER)S0278-6915(19)30680-5 DE-627 ger DE-627 rakwb eng 530 620 VZ 52.56 bkl Lu, Jing verfasserin aut Co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in Bacillus subtilis for alcohol detoxification 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. Bacillus subtilis Elsevier Alcohol detoxification Elsevier Alcohol dehydrogenase Elsevier Aldehyde dehydrogenase Elsevier Alcohol-induced liver injury Elsevier Zhu, Xiaoyu oth Zhang, Chong oth Lu, Fengxia oth Lu, Zhaoxin oth Lu, Yingjian oth Enthalten in Elsevier Manatbayev, Rustem ELSEVIER Numerical simulations on static Vertical Axis Wind Turbine blade icing 2021 New York, NY [u.a.] (DE-627)ELV005709407 volume:135 year:2020 pages:0 https://doi.org/10.1016/j.fct.2019.110890 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 52.56 Regenerative Energieformen alternative Energieformen VZ AR 135 2020 0 |
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10.1016/j.fct.2019.110890 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000000887.pica (DE-627)ELV048918954 (ELSEVIER)S0278-6915(19)30680-5 DE-627 ger DE-627 rakwb eng 530 620 VZ 52.56 bkl Lu, Jing verfasserin aut Co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in Bacillus subtilis for alcohol detoxification 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. Bacillus subtilis Elsevier Alcohol detoxification Elsevier Alcohol dehydrogenase Elsevier Aldehyde dehydrogenase Elsevier Alcohol-induced liver injury Elsevier Zhu, Xiaoyu oth Zhang, Chong oth Lu, Fengxia oth Lu, Zhaoxin oth Lu, Yingjian oth Enthalten in Elsevier Manatbayev, Rustem ELSEVIER Numerical simulations on static Vertical Axis Wind Turbine blade icing 2021 New York, NY [u.a.] (DE-627)ELV005709407 volume:135 year:2020 pages:0 https://doi.org/10.1016/j.fct.2019.110890 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 52.56 Regenerative Energieformen alternative Energieformen VZ AR 135 2020 0 |
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10.1016/j.fct.2019.110890 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000000887.pica (DE-627)ELV048918954 (ELSEVIER)S0278-6915(19)30680-5 DE-627 ger DE-627 rakwb eng 530 620 VZ 52.56 bkl Lu, Jing verfasserin aut Co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in Bacillus subtilis for alcohol detoxification 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. Bacillus subtilis Elsevier Alcohol detoxification Elsevier Alcohol dehydrogenase Elsevier Aldehyde dehydrogenase Elsevier Alcohol-induced liver injury Elsevier Zhu, Xiaoyu oth Zhang, Chong oth Lu, Fengxia oth Lu, Zhaoxin oth Lu, Yingjian oth Enthalten in Elsevier Manatbayev, Rustem ELSEVIER Numerical simulations on static Vertical Axis Wind Turbine blade icing 2021 New York, NY [u.a.] (DE-627)ELV005709407 volume:135 year:2020 pages:0 https://doi.org/10.1016/j.fct.2019.110890 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 52.56 Regenerative Energieformen alternative Energieformen VZ AR 135 2020 0 |
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co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in bacillus subtilis for alcohol detoxification |
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Co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in Bacillus subtilis for alcohol detoxification |
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Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. |
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Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. |
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Alcoholic liver disease is a major cause of morbidity and mortality worldwide. In this work, a food-grade recombinant Bacillus subtilis co-expressing both alcohol dehydrogenase from Saccharomyces cerevisiae (scADH) and aldehyde dehydrogenase from Issatchenkia terricola (istALDH) were successfully constructed via double-crossover homologous recombination. When cultured at 37 °C for 48 h, the activities of scADH and istALDH were 57.56 ± 7.44 and 81.41 ± 8.26 U/mL, respectively. In pH 4.0, the alcohol degradation rate of recombinant B. subtilis fmb8 was 33% and the ΔLog10 was 0.1, indicating that fmb8 could be used as whole-cell biocatalysts for biodegradation of alcohols under low pH conditions. Mice experiments indicated that recombinant B. subtilis significantly alleviate recombinant alcohol-induced increases of mouse liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, recombinant B. subtilis significantly reduced liver malondialdehyde levels and increased total antioxidant capacity and superoxide dismutase levels in mouse liver. Overall, our findings suggested that food-grade B. subtilis fmb8 co-expressing scADH and istALDH could be used as a potential probiotic for alcohol detoxification and alleviation of alcoholic liver injury. |
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