Ultrasonic disruption extraction, characterization and bioactivities of polysaccharides from wild Armillaria mellea
An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Chen, Ruizhan [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020transfer abstract |
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| Umfang: |
12 |
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| Übergeordnetes Werk: |
Enthalten in: Automated DNA hybridization transfer with movable super-paramagnetic microbeads in a microflow reactor - Penchovsky, Robert ELSEVIER, 2019, structure, function and interactions, New York, NY [u.a.] |
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| Übergeordnetes Werk: |
volume:156 ; year:2020 ; day:1 ; month:08 ; pages:1491-1502 ; extent:12 |
| Links: |
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| DOI / URN: |
10.1016/j.ijbiomac.2019.11.196 |
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ELV050467174 |
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| 245 | 1 | 0 | |a Ultrasonic disruption extraction, characterization and bioactivities of polysaccharides from wild Armillaria mellea |
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| 520 | |a An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. | ||
| 520 | |a An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. | ||
| 700 | 1 | |a Ren, Xing |4 oth | |
| 700 | 1 | |a Yin, Wei |4 oth | |
| 700 | 1 | |a Lu, Juan |4 oth | |
| 700 | 1 | |a Tian, Li |4 oth | |
| 700 | 1 | |a Zhao, Lun |4 oth | |
| 700 | 1 | |a Yang, Ruping |4 oth | |
| 700 | 1 | |a Luo, Shujun |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier |a Penchovsky, Robert ELSEVIER |t Automated DNA hybridization transfer with movable super-paramagnetic microbeads in a microflow reactor |d 2019 |d structure, function and interactions |g New York, NY [u.a.] |w (DE-627)ELV002200198 |
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10.1016/j.ijbiomac.2019.11.196 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001024.pica (DE-627)ELV050467174 (ELSEVIER)S0141-8130(19)38360-6 DE-627 ger DE-627 rakwb eng 570 610 VZ 58.30 bkl 50.22 bkl 44.09 bkl Chen, Ruizhan verfasserin aut Ultrasonic disruption extraction, characterization and bioactivities of polysaccharides from wild Armillaria mellea 2020transfer abstract 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. Ren, Xing oth Yin, Wei oth Lu, Juan oth Tian, Li oth Zhao, Lun oth Yang, Ruping oth Luo, Shujun oth Enthalten in Elsevier Penchovsky, Robert ELSEVIER Automated DNA hybridization transfer with movable super-paramagnetic microbeads in a microflow reactor 2019 structure, function and interactions New York, NY [u.a.] (DE-627)ELV002200198 volume:156 year:2020 day:1 month:08 pages:1491-1502 extent:12 https://doi.org/10.1016/j.ijbiomac.2019.11.196 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 58.30 Biotechnologie VZ 50.22 Sensorik VZ 44.09 Medizintechnik VZ AR 156 2020 1 0801 1491-1502 12 |
| spelling |
10.1016/j.ijbiomac.2019.11.196 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001024.pica (DE-627)ELV050467174 (ELSEVIER)S0141-8130(19)38360-6 DE-627 ger DE-627 rakwb eng 570 610 VZ 58.30 bkl 50.22 bkl 44.09 bkl Chen, Ruizhan verfasserin aut Ultrasonic disruption extraction, characterization and bioactivities of polysaccharides from wild Armillaria mellea 2020transfer abstract 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. Ren, Xing oth Yin, Wei oth Lu, Juan oth Tian, Li oth Zhao, Lun oth Yang, Ruping oth Luo, Shujun oth Enthalten in Elsevier Penchovsky, Robert ELSEVIER Automated DNA hybridization transfer with movable super-paramagnetic microbeads in a microflow reactor 2019 structure, function and interactions New York, NY [u.a.] (DE-627)ELV002200198 volume:156 year:2020 day:1 month:08 pages:1491-1502 extent:12 https://doi.org/10.1016/j.ijbiomac.2019.11.196 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 58.30 Biotechnologie VZ 50.22 Sensorik VZ 44.09 Medizintechnik VZ AR 156 2020 1 0801 1491-1502 12 |
| allfields_unstemmed |
10.1016/j.ijbiomac.2019.11.196 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001024.pica (DE-627)ELV050467174 (ELSEVIER)S0141-8130(19)38360-6 DE-627 ger DE-627 rakwb eng 570 610 VZ 58.30 bkl 50.22 bkl 44.09 bkl Chen, Ruizhan verfasserin aut Ultrasonic disruption extraction, characterization and bioactivities of polysaccharides from wild Armillaria mellea 2020transfer abstract 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. Ren, Xing oth Yin, Wei oth Lu, Juan oth Tian, Li oth Zhao, Lun oth Yang, Ruping oth Luo, Shujun oth Enthalten in Elsevier Penchovsky, Robert ELSEVIER Automated DNA hybridization transfer with movable super-paramagnetic microbeads in a microflow reactor 2019 structure, function and interactions New York, NY [u.a.] (DE-627)ELV002200198 volume:156 year:2020 day:1 month:08 pages:1491-1502 extent:12 https://doi.org/10.1016/j.ijbiomac.2019.11.196 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 58.30 Biotechnologie VZ 50.22 Sensorik VZ 44.09 Medizintechnik VZ AR 156 2020 1 0801 1491-1502 12 |
| allfieldsGer |
10.1016/j.ijbiomac.2019.11.196 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001024.pica (DE-627)ELV050467174 (ELSEVIER)S0141-8130(19)38360-6 DE-627 ger DE-627 rakwb eng 570 610 VZ 58.30 bkl 50.22 bkl 44.09 bkl Chen, Ruizhan verfasserin aut Ultrasonic disruption extraction, characterization and bioactivities of polysaccharides from wild Armillaria mellea 2020transfer abstract 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. Ren, Xing oth Yin, Wei oth Lu, Juan oth Tian, Li oth Zhao, Lun oth Yang, Ruping oth Luo, Shujun oth Enthalten in Elsevier Penchovsky, Robert ELSEVIER Automated DNA hybridization transfer with movable super-paramagnetic microbeads in a microflow reactor 2019 structure, function and interactions New York, NY [u.a.] (DE-627)ELV002200198 volume:156 year:2020 day:1 month:08 pages:1491-1502 extent:12 https://doi.org/10.1016/j.ijbiomac.2019.11.196 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 58.30 Biotechnologie VZ 50.22 Sensorik VZ 44.09 Medizintechnik VZ AR 156 2020 1 0801 1491-1502 12 |
| allfieldsSound |
10.1016/j.ijbiomac.2019.11.196 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001024.pica (DE-627)ELV050467174 (ELSEVIER)S0141-8130(19)38360-6 DE-627 ger DE-627 rakwb eng 570 610 VZ 58.30 bkl 50.22 bkl 44.09 bkl Chen, Ruizhan verfasserin aut Ultrasonic disruption extraction, characterization and bioactivities of polysaccharides from wild Armillaria mellea 2020transfer abstract 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. Ren, Xing oth Yin, Wei oth Lu, Juan oth Tian, Li oth Zhao, Lun oth Yang, Ruping oth Luo, Shujun oth Enthalten in Elsevier Penchovsky, Robert ELSEVIER Automated DNA hybridization transfer with movable super-paramagnetic microbeads in a microflow reactor 2019 structure, function and interactions New York, NY [u.a.] (DE-627)ELV002200198 volume:156 year:2020 day:1 month:08 pages:1491-1502 extent:12 https://doi.org/10.1016/j.ijbiomac.2019.11.196 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 58.30 Biotechnologie VZ 50.22 Sensorik VZ 44.09 Medizintechnik VZ AR 156 2020 1 0801 1491-1502 12 |
| language |
English |
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Ultrasonic disruption extraction, characterization and bioactivities of polysaccharides from wild Armillaria mellea |
| abstract |
An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. |
| abstractGer |
An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. |
| abstract_unstemmed |
An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents. |
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AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">An efficient ultrasonic disruption extraction (UDE) of polysaccharides from Armillaria mellea (AM) were optimized by response surface methodology (RSM). Under optimum conditions: ultrasonic power 915.00 W, temperature 69.27 °C and time 39.13 min, the crude polysaccharides (AMPs) yield was 19.5%. Two purified fractions AMPs-1-1 and AMPs-2-1 were obtained through anion-exchange and gel chromatography. AMPs-1-1 was a heteropolysaccharide with average molecular weights of 1.23 × 105 Da, and composed of Glc, Gal and GlcA with mole percentages of 89.06%, 9.59% and 1.34%, respectively, owning a backbone structure of (1→)-β-d-Glcp, (1 → 3,6)-α-d-Glcp and (1 → 3)-β-d-Glcp residues. AMPs-2-1 was a heteropolysaccharide with average molecular weights of 6.76 × 104 Da, and composed of Glc, Gal, GlcA and Man with mole percentages of 65.28%, 22.87%, 2.87% and 8.98%, containing a main backbone chain of (1 → 3,6)-α-d-Glcp and (1 → 6)-β-d-Glcp residues. AMPs-2-1 possessed obviously antioxidant activities in terms of stronger scavenging activity against DPPH· and ABTS+⋅, higher FRAP and ORAC value than AMPs-1-1. AMPs-2-1 could promote splenocyte lymphocytes and RAW264.7 macrophages proliferation and enhanced the phagocytosis of macrophages, exhibited significant immunomodulatory activities. These results suggested that UDE is an effective extract technology, and AMPs-2-1 could be explored as potential natural antioxidants and immunomodulatory agents.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ren, Xing</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yin, Wei</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Juan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tian, Li</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhao, Lun</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Ruping</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Luo, Shujun</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier</subfield><subfield code="a">Penchovsky, Robert ELSEVIER</subfield><subfield code="t">Automated DNA hybridization transfer with movable super-paramagnetic microbeads in a microflow reactor</subfield><subfield code="d">2019</subfield><subfield code="d">structure, function and interactions</subfield><subfield code="g">New York, NY [u.a.]</subfield><subfield code="w">(DE-627)ELV002200198</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:156</subfield><subfield code="g">year:2020</subfield><subfield code="g">day:1</subfield><subfield code="g">month:08</subfield><subfield code="g">pages:1491-1502</subfield><subfield code="g">extent:12</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.ijbiomac.2019.11.196</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">58.30</subfield><subfield code="j">Biotechnologie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">50.22</subfield><subfield code="j">Sensorik</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.09</subfield><subfield code="j">Medizintechnik</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">156</subfield><subfield code="j">2020</subfield><subfield code="b">1</subfield><subfield code="c">0801</subfield><subfield code="h">1491-1502</subfield><subfield code="g">12</subfield></datafield></record></collection>
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