Interleukin-dependent modulation of the expression of MHC class I and MHC class II genes in chicken HD11 cells
Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we ev...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Truong, Anh Duc [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis - Wearne, Travis A. ELSEVIER, 2016transfer abstract, ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:110 ; year:2020 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.dci.2020.103729 |
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| 245 | 1 | 0 | |a Interleukin-dependent modulation of the expression of MHC class I and MHC class II genes in chicken HD11 cells |
| 264 | 1 | |c 2020transfer abstract | |
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| 520 | |a Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. | ||
| 520 | |a Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. | ||
| 650 | 7 | |a NF-κB |2 Elsevier | |
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| 650 | 7 | |a MHC class II |2 Elsevier | |
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| 700 | 1 | |a Ly, Viet Duc |4 oth | |
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| 700 | 1 | |a Van Hoang, Tuan |4 oth | |
| 700 | 1 | |a Thanh Tran, Ha Thi |4 oth | |
| 700 | 1 | |a Dang, Hoang Vu |4 oth | |
| 700 | 1 | |a Hong, Yeong Ho |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Wearne, Travis A. ELSEVIER |t GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis |d 2016transfer abstract |d ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology |g Amsterdam [u.a.] |w (DE-627)ELV019920881 |
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10.1016/j.dci.2020.103729 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001028.pica (DE-627)ELV050514407 (ELSEVIER)S0145-305X(20)30131-2 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Truong, Anh Duc verfasserin aut Interleukin-dependent modulation of the expression of MHC class I and MHC class II genes in chicken HD11 cells 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. NF-κB Elsevier Interleukin Elsevier MHC class I Elsevier MHC class II Elsevier Macrophage Elsevier Hong, Yeojin oth Ly, Viet Duc oth Nguyen, Huyen Thi oth Nguyen, Chinh Thi oth Vu, Hao Thi oth Chu, Nhu Thi oth Van Hoang, Tuan oth Thanh Tran, Ha Thi oth Dang, Hoang Vu oth Hong, Yeong Ho oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:110 year:2020 pages:0 https://doi.org/10.1016/j.dci.2020.103729 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 110 2020 0 |
| spelling |
10.1016/j.dci.2020.103729 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001028.pica (DE-627)ELV050514407 (ELSEVIER)S0145-305X(20)30131-2 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Truong, Anh Duc verfasserin aut Interleukin-dependent modulation of the expression of MHC class I and MHC class II genes in chicken HD11 cells 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. NF-κB Elsevier Interleukin Elsevier MHC class I Elsevier MHC class II Elsevier Macrophage Elsevier Hong, Yeojin oth Ly, Viet Duc oth Nguyen, Huyen Thi oth Nguyen, Chinh Thi oth Vu, Hao Thi oth Chu, Nhu Thi oth Van Hoang, Tuan oth Thanh Tran, Ha Thi oth Dang, Hoang Vu oth Hong, Yeong Ho oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:110 year:2020 pages:0 https://doi.org/10.1016/j.dci.2020.103729 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 110 2020 0 |
| allfields_unstemmed |
10.1016/j.dci.2020.103729 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001028.pica (DE-627)ELV050514407 (ELSEVIER)S0145-305X(20)30131-2 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Truong, Anh Duc verfasserin aut Interleukin-dependent modulation of the expression of MHC class I and MHC class II genes in chicken HD11 cells 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. NF-κB Elsevier Interleukin Elsevier MHC class I Elsevier MHC class II Elsevier Macrophage Elsevier Hong, Yeojin oth Ly, Viet Duc oth Nguyen, Huyen Thi oth Nguyen, Chinh Thi oth Vu, Hao Thi oth Chu, Nhu Thi oth Van Hoang, Tuan oth Thanh Tran, Ha Thi oth Dang, Hoang Vu oth Hong, Yeong Ho oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:110 year:2020 pages:0 https://doi.org/10.1016/j.dci.2020.103729 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 110 2020 0 |
| allfieldsGer |
10.1016/j.dci.2020.103729 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001028.pica (DE-627)ELV050514407 (ELSEVIER)S0145-305X(20)30131-2 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Truong, Anh Duc verfasserin aut Interleukin-dependent modulation of the expression of MHC class I and MHC class II genes in chicken HD11 cells 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. NF-κB Elsevier Interleukin Elsevier MHC class I Elsevier MHC class II Elsevier Macrophage Elsevier Hong, Yeojin oth Ly, Viet Duc oth Nguyen, Huyen Thi oth Nguyen, Chinh Thi oth Vu, Hao Thi oth Chu, Nhu Thi oth Van Hoang, Tuan oth Thanh Tran, Ha Thi oth Dang, Hoang Vu oth Hong, Yeong Ho oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:110 year:2020 pages:0 https://doi.org/10.1016/j.dci.2020.103729 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 110 2020 0 |
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10.1016/j.dci.2020.103729 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001028.pica (DE-627)ELV050514407 (ELSEVIER)S0145-305X(20)30131-2 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Truong, Anh Duc verfasserin aut Interleukin-dependent modulation of the expression of MHC class I and MHC class II genes in chicken HD11 cells 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. NF-κB Elsevier Interleukin Elsevier MHC class I Elsevier MHC class II Elsevier Macrophage Elsevier Hong, Yeojin oth Ly, Viet Duc oth Nguyen, Huyen Thi oth Nguyen, Chinh Thi oth Vu, Hao Thi oth Chu, Nhu Thi oth Van Hoang, Tuan oth Thanh Tran, Ha Thi oth Dang, Hoang Vu oth Hong, Yeong Ho oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:110 year:2020 pages:0 https://doi.org/10.1016/j.dci.2020.103729 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 110 2020 0 |
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Interleukin-dependent modulation of the expression of MHC class I and MHC class II genes in chicken HD11 cells |
| abstract |
Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. |
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Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. |
| abstract_unstemmed |
Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins—i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34—on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production. |
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| title_short |
Interleukin-dependent modulation of the expression of MHC class I and MHC class II genes in chicken HD11 cells |
| url |
https://doi.org/10.1016/j.dci.2020.103729 |
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Hong, Yeojin Ly, Viet Duc Nguyen, Huyen Thi Nguyen, Chinh Thi Vu, Hao Thi Chu, Nhu Thi Van Hoang, Tuan Thanh Tran, Ha Thi Dang, Hoang Vu Hong, Yeong Ho |
| author2Str |
Hong, Yeojin Ly, Viet Duc Nguyen, Huyen Thi Nguyen, Chinh Thi Vu, Hao Thi Chu, Nhu Thi Van Hoang, Tuan Thanh Tran, Ha Thi Dang, Hoang Vu Hong, Yeong Ho |
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| doi_str |
10.1016/j.dci.2020.103729 |
| up_date |
2024-07-06T17:44:40.827Z |
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