The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis
The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mi...
Ausführliche Beschreibung
Autor*in: |
Huang, Shu [verfasserIn] |
---|
Format: |
E-Artikel |
---|---|
Sprache: |
Englisch |
Erschienen: |
2020transfer abstract |
---|
Schlagwörter: |
---|
Übergeordnetes Werk: |
Enthalten in: GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis - Wearne, Travis A. ELSEVIER, 2016transfer abstract, ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology, Amsterdam [u.a.] |
---|---|
Übergeordnetes Werk: |
volume:111 ; year:2020 ; pages:0 |
Links: |
---|
DOI / URN: |
10.1016/j.dci.2020.103757 |
---|
Katalog-ID: |
ELV050886940 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | ELV050886940 | ||
003 | DE-627 | ||
005 | 20230626031251.0 | ||
007 | cr uuu---uuuuu | ||
008 | 210910s2020 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.dci.2020.103757 |2 doi | |
028 | 5 | 2 | |a /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001079.pica |
035 | |a (DE-627)ELV050886940 | ||
035 | |a (ELSEVIER)S0145-305X(20)30099-9 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | 4 | |a 610 |q VZ |
082 | 0 | 4 | |a 610 |q VZ |
084 | |a 44.88 |2 bkl | ||
100 | 1 | |a Huang, Shu |e verfasserin |4 aut | |
245 | 1 | 4 | |a The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis |
264 | 1 | |c 2020transfer abstract | |
336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
337 | |a nicht spezifiziert |b z |2 rdamedia | ||
338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
520 | |a The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. | ||
520 | |a The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. | ||
650 | 7 | |a Antimicrobial peptides |2 Elsevier | |
650 | 7 | |a Ecdysone |2 Elsevier | |
650 | 7 | |a Eriocheir sinensis |2 Elsevier | |
650 | 7 | |a Ecdysone receptor |2 Elsevier | |
700 | 1 | |a Yi, Qilin |4 oth | |
700 | 1 | |a Lian, Xingye |4 oth | |
700 | 1 | |a Xu, Siqi |4 oth | |
700 | 1 | |a Yang, Chuanyan |4 oth | |
700 | 1 | |a Sun, Jiejie |4 oth | |
700 | 1 | |a Wang, Lingling |4 oth | |
700 | 1 | |a Song, Linsheng |4 oth | |
773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Wearne, Travis A. ELSEVIER |t GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis |d 2016transfer abstract |d ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology |g Amsterdam [u.a.] |w (DE-627)ELV019920881 |
773 | 1 | 8 | |g volume:111 |g year:2020 |g pages:0 |
856 | 4 | 0 | |u https://doi.org/10.1016/j.dci.2020.103757 |3 Volltext |
912 | |a GBV_USEFLAG_U | ||
912 | |a GBV_ELV | ||
912 | |a SYSFLAG_U | ||
912 | |a SSG-OLC-PHA | ||
912 | |a GBV_ILN_60 | ||
936 | b | k | |a 44.88 |j Urologie |j Nephrologie |q VZ |
951 | |a AR | ||
952 | |d 111 |j 2020 |h 0 |
author_variant |
s h sh |
---|---|
matchkey_str |
huangshuyiqilinlianxingyexusiqiyangchuan:2020----:hivleetfcyoenedsnrcpoirgltntexrsinfniirbappiei |
hierarchy_sort_str |
2020transfer abstract |
bklnumber |
44.88 |
publishDate |
2020 |
allfields |
10.1016/j.dci.2020.103757 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001079.pica (DE-627)ELV050886940 (ELSEVIER)S0145-305X(20)30099-9 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Huang, Shu verfasserin aut The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. Antimicrobial peptides Elsevier Ecdysone Elsevier Eriocheir sinensis Elsevier Ecdysone receptor Elsevier Yi, Qilin oth Lian, Xingye oth Xu, Siqi oth Yang, Chuanyan oth Sun, Jiejie oth Wang, Lingling oth Song, Linsheng oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:111 year:2020 pages:0 https://doi.org/10.1016/j.dci.2020.103757 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 111 2020 0 |
spelling |
10.1016/j.dci.2020.103757 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001079.pica (DE-627)ELV050886940 (ELSEVIER)S0145-305X(20)30099-9 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Huang, Shu verfasserin aut The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. Antimicrobial peptides Elsevier Ecdysone Elsevier Eriocheir sinensis Elsevier Ecdysone receptor Elsevier Yi, Qilin oth Lian, Xingye oth Xu, Siqi oth Yang, Chuanyan oth Sun, Jiejie oth Wang, Lingling oth Song, Linsheng oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:111 year:2020 pages:0 https://doi.org/10.1016/j.dci.2020.103757 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 111 2020 0 |
allfields_unstemmed |
10.1016/j.dci.2020.103757 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001079.pica (DE-627)ELV050886940 (ELSEVIER)S0145-305X(20)30099-9 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Huang, Shu verfasserin aut The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. Antimicrobial peptides Elsevier Ecdysone Elsevier Eriocheir sinensis Elsevier Ecdysone receptor Elsevier Yi, Qilin oth Lian, Xingye oth Xu, Siqi oth Yang, Chuanyan oth Sun, Jiejie oth Wang, Lingling oth Song, Linsheng oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:111 year:2020 pages:0 https://doi.org/10.1016/j.dci.2020.103757 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 111 2020 0 |
allfieldsGer |
10.1016/j.dci.2020.103757 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001079.pica (DE-627)ELV050886940 (ELSEVIER)S0145-305X(20)30099-9 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Huang, Shu verfasserin aut The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. Antimicrobial peptides Elsevier Ecdysone Elsevier Eriocheir sinensis Elsevier Ecdysone receptor Elsevier Yi, Qilin oth Lian, Xingye oth Xu, Siqi oth Yang, Chuanyan oth Sun, Jiejie oth Wang, Lingling oth Song, Linsheng oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:111 year:2020 pages:0 https://doi.org/10.1016/j.dci.2020.103757 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 111 2020 0 |
allfieldsSound |
10.1016/j.dci.2020.103757 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001079.pica (DE-627)ELV050886940 (ELSEVIER)S0145-305X(20)30099-9 DE-627 ger DE-627 rakwb eng 610 VZ 610 VZ 44.88 bkl Huang, Shu verfasserin aut The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis 2020transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. Antimicrobial peptides Elsevier Ecdysone Elsevier Eriocheir sinensis Elsevier Ecdysone receptor Elsevier Yi, Qilin oth Lian, Xingye oth Xu, Siqi oth Yang, Chuanyan oth Sun, Jiejie oth Wang, Lingling oth Song, Linsheng oth Enthalten in Elsevier Science Wearne, Travis A. ELSEVIER GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis 2016transfer abstract ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology Amsterdam [u.a.] (DE-627)ELV019920881 volume:111 year:2020 pages:0 https://doi.org/10.1016/j.dci.2020.103757 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 44.88 Urologie Nephrologie VZ AR 111 2020 0 |
language |
English |
source |
Enthalten in GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis Amsterdam [u.a.] volume:111 year:2020 pages:0 |
sourceStr |
Enthalten in GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis Amsterdam [u.a.] volume:111 year:2020 pages:0 |
format_phy_str_mv |
Article |
bklname |
Urologie Nephrologie |
institution |
findex.gbv.de |
topic_facet |
Antimicrobial peptides Ecdysone Eriocheir sinensis Ecdysone receptor |
dewey-raw |
610 |
isfreeaccess_bool |
false |
container_title |
GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis |
authorswithroles_txt_mv |
Huang, Shu @@aut@@ Yi, Qilin @@oth@@ Lian, Xingye @@oth@@ Xu, Siqi @@oth@@ Yang, Chuanyan @@oth@@ Sun, Jiejie @@oth@@ Wang, Lingling @@oth@@ Song, Linsheng @@oth@@ |
publishDateDaySort_date |
2020-01-01T00:00:00Z |
hierarchy_top_id |
ELV019920881 |
dewey-sort |
3610 |
id |
ELV050886940 |
language_de |
englisch |
fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV050886940</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230626031251.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">210910s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.dci.2020.103757</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">/cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001079.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV050886940</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0145-305X(20)30099-9</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.88</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Huang, Shu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020transfer abstract</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Antimicrobial peptides</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Ecdysone</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Eriocheir sinensis</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Ecdysone receptor</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yi, Qilin</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lian, Xingye</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Siqi</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Chuanyan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sun, Jiejie</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Lingling</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Song, Linsheng</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier Science</subfield><subfield code="a">Wearne, Travis A. ELSEVIER</subfield><subfield code="t">GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis</subfield><subfield code="d">2016transfer abstract</subfield><subfield code="d">ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV019920881</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:111</subfield><subfield code="g">year:2020</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.dci.2020.103757</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.88</subfield><subfield code="j">Urologie</subfield><subfield code="j">Nephrologie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">111</subfield><subfield code="j">2020</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
author |
Huang, Shu |
spellingShingle |
Huang, Shu ddc 610 bkl 44.88 Elsevier Antimicrobial peptides Elsevier Ecdysone Elsevier Eriocheir sinensis Elsevier Ecdysone receptor The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis |
authorStr |
Huang, Shu |
ppnlink_with_tag_str_mv |
@@773@@(DE-627)ELV019920881 |
format |
electronic Article |
dewey-ones |
610 - Medicine & health |
delete_txt_mv |
keep |
author_role |
aut |
collection |
elsevier |
remote_str |
true |
illustrated |
Not Illustrated |
topic_title |
610 VZ 44.88 bkl The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis Antimicrobial peptides Elsevier Ecdysone Elsevier Eriocheir sinensis Elsevier Ecdysone receptor Elsevier |
topic |
ddc 610 bkl 44.88 Elsevier Antimicrobial peptides Elsevier Ecdysone Elsevier Eriocheir sinensis Elsevier Ecdysone receptor |
topic_unstemmed |
ddc 610 bkl 44.88 Elsevier Antimicrobial peptides Elsevier Ecdysone Elsevier Eriocheir sinensis Elsevier Ecdysone receptor |
topic_browse |
ddc 610 bkl 44.88 Elsevier Antimicrobial peptides Elsevier Ecdysone Elsevier Eriocheir sinensis Elsevier Ecdysone receptor |
format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
format_main_str_mv |
Text Zeitschrift/Artikel |
carriertype_str_mv |
zu |
author2_variant |
q y qy x l xl s x sx c y cy j s js l w lw l s ls |
hierarchy_parent_title |
GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis |
hierarchy_parent_id |
ELV019920881 |
dewey-tens |
610 - Medicine & health |
hierarchy_top_title |
GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis |
isfreeaccess_txt |
false |
familylinks_str_mv |
(DE-627)ELV019920881 |
title |
The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis |
ctrlnum |
(DE-627)ELV050886940 (ELSEVIER)S0145-305X(20)30099-9 |
title_full |
The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis |
author_sort |
Huang, Shu |
journal |
GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis |
journalStr |
GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis |
lang_code |
eng |
isOA_bool |
false |
dewey-hundreds |
600 - Technology |
recordtype |
marc |
publishDateSort |
2020 |
contenttype_str_mv |
zzz |
container_start_page |
0 |
author_browse |
Huang, Shu |
container_volume |
111 |
class |
610 VZ 44.88 bkl |
format_se |
Elektronische Aufsätze |
author-letter |
Huang, Shu |
doi_str_mv |
10.1016/j.dci.2020.103757 |
dewey-full |
610 |
title_sort |
involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in chinese mitten crab, eriocheir sinensis |
title_auth |
The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis |
abstract |
The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. |
abstractGer |
The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. |
abstract_unstemmed |
The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK. |
collection_details |
GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_60 |
title_short |
The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis |
url |
https://doi.org/10.1016/j.dci.2020.103757 |
remote_bool |
true |
author2 |
Yi, Qilin Lian, Xingye Xu, Siqi Yang, Chuanyan Sun, Jiejie Wang, Lingling Song, Linsheng |
author2Str |
Yi, Qilin Lian, Xingye Xu, Siqi Yang, Chuanyan Sun, Jiejie Wang, Lingling Song, Linsheng |
ppnlink |
ELV019920881 |
mediatype_str_mv |
z |
isOA_txt |
false |
hochschulschrift_bool |
false |
author2_role |
oth oth oth oth oth oth oth |
doi_str |
10.1016/j.dci.2020.103757 |
up_date |
2024-07-06T18:44:23.339Z |
_version_ |
1803856341464252416 |
fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV050886940</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230626031251.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">210910s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.dci.2020.103757</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">/cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001079.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV050886940</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0145-305X(20)30099-9</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.88</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Huang, Shu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020transfer abstract</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Antimicrobial peptides</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Ecdysone</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Eriocheir sinensis</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Ecdysone receptor</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yi, Qilin</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lian, Xingye</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Siqi</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Chuanyan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sun, Jiejie</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Lingling</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Song, Linsheng</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier Science</subfield><subfield code="a">Wearne, Travis A. ELSEVIER</subfield><subfield code="t">GABAergic mRNA expression is differentially expressed across the prelimbic and orbitofrontal cortices of rats sensitized to methamphetamine: Relevance to psychosis</subfield><subfield code="d">2016transfer abstract</subfield><subfield code="d">ontogeny, phylogeny, aging : the official journal of the International Society of Developmental and Comparative Immunology</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV019920881</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:111</subfield><subfield code="g">year:2020</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.dci.2020.103757</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.88</subfield><subfield code="j">Urologie</subfield><subfield code="j">Nephrologie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">111</subfield><subfield code="j">2020</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
score |
7.4009905 |