The link between delivered aerosol dose and inflammatory responses: Exposing a lung Cell Co-Culture system to selected Allergens and irritants
Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary inform...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Sayes, Christie M. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: The information content of the conditional pair probability - Rincón, Luis ELSEVIER, 2015, an international journal, Amsterdam |
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| Übergeordnetes Werk: |
volume:151 ; year:2021 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.jaerosci.2020.105677 |
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ELV052012573 |
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| 245 | 1 | 4 | |a The link between delivered aerosol dose and inflammatory responses: Exposing a lung Cell Co-Culture system to selected Allergens and irritants |
| 264 | 1 | |c 2021transfer abstract | |
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| 520 | |a Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. | ||
| 520 | |a Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. | ||
| 650 | 7 | |a Aerosol engineering |2 Elsevier | |
| 650 | 7 | |a Respiratory cell culture |2 Elsevier | |
| 650 | 7 | |a Air-liquid interface |2 Elsevier | |
| 650 | 7 | |a In vitro exposure System |2 Elsevier | |
| 650 | 7 | |a Dosimetry |2 Elsevier | |
| 700 | 1 | |a Singal, Madhuri |4 oth | |
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10.1016/j.jaerosci.2020.105677 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001295.pica (DE-627)ELV052012573 (ELSEVIER)S0021-8502(20)30163-4 DE-627 ger DE-627 rakwb eng 540 VZ 660 VZ 660 VZ 58.10 bkl Sayes, Christie M. verfasserin aut The link between delivered aerosol dose and inflammatory responses: Exposing a lung Cell Co-Culture system to selected Allergens and irritants 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. Aerosol engineering Elsevier Respiratory cell culture Elsevier Air-liquid interface Elsevier In vitro exposure System Elsevier Dosimetry Elsevier Singal, Madhuri oth Enthalten in Elsevier Rincón, Luis ELSEVIER The information content of the conditional pair probability 2015 an international journal Amsterdam (DE-627)ELV023917989 volume:151 year:2021 pages:0 https://doi.org/10.1016/j.jaerosci.2020.105677 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_72 58.10 Verfahrenstechnik: Allgemeines VZ AR 151 2021 0 |
| spelling |
10.1016/j.jaerosci.2020.105677 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001295.pica (DE-627)ELV052012573 (ELSEVIER)S0021-8502(20)30163-4 DE-627 ger DE-627 rakwb eng 540 VZ 660 VZ 660 VZ 58.10 bkl Sayes, Christie M. verfasserin aut The link between delivered aerosol dose and inflammatory responses: Exposing a lung Cell Co-Culture system to selected Allergens and irritants 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. Aerosol engineering Elsevier Respiratory cell culture Elsevier Air-liquid interface Elsevier In vitro exposure System Elsevier Dosimetry Elsevier Singal, Madhuri oth Enthalten in Elsevier Rincón, Luis ELSEVIER The information content of the conditional pair probability 2015 an international journal Amsterdam (DE-627)ELV023917989 volume:151 year:2021 pages:0 https://doi.org/10.1016/j.jaerosci.2020.105677 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_72 58.10 Verfahrenstechnik: Allgemeines VZ AR 151 2021 0 |
| allfields_unstemmed |
10.1016/j.jaerosci.2020.105677 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001295.pica (DE-627)ELV052012573 (ELSEVIER)S0021-8502(20)30163-4 DE-627 ger DE-627 rakwb eng 540 VZ 660 VZ 660 VZ 58.10 bkl Sayes, Christie M. verfasserin aut The link between delivered aerosol dose and inflammatory responses: Exposing a lung Cell Co-Culture system to selected Allergens and irritants 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. Aerosol engineering Elsevier Respiratory cell culture Elsevier Air-liquid interface Elsevier In vitro exposure System Elsevier Dosimetry Elsevier Singal, Madhuri oth Enthalten in Elsevier Rincón, Luis ELSEVIER The information content of the conditional pair probability 2015 an international journal Amsterdam (DE-627)ELV023917989 volume:151 year:2021 pages:0 https://doi.org/10.1016/j.jaerosci.2020.105677 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_72 58.10 Verfahrenstechnik: Allgemeines VZ AR 151 2021 0 |
| allfieldsGer |
10.1016/j.jaerosci.2020.105677 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001295.pica (DE-627)ELV052012573 (ELSEVIER)S0021-8502(20)30163-4 DE-627 ger DE-627 rakwb eng 540 VZ 660 VZ 660 VZ 58.10 bkl Sayes, Christie M. verfasserin aut The link between delivered aerosol dose and inflammatory responses: Exposing a lung Cell Co-Culture system to selected Allergens and irritants 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. Aerosol engineering Elsevier Respiratory cell culture Elsevier Air-liquid interface Elsevier In vitro exposure System Elsevier Dosimetry Elsevier Singal, Madhuri oth Enthalten in Elsevier Rincón, Luis ELSEVIER The information content of the conditional pair probability 2015 an international journal Amsterdam (DE-627)ELV023917989 volume:151 year:2021 pages:0 https://doi.org/10.1016/j.jaerosci.2020.105677 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_72 58.10 Verfahrenstechnik: Allgemeines VZ AR 151 2021 0 |
| allfieldsSound |
10.1016/j.jaerosci.2020.105677 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001295.pica (DE-627)ELV052012573 (ELSEVIER)S0021-8502(20)30163-4 DE-627 ger DE-627 rakwb eng 540 VZ 660 VZ 660 VZ 58.10 bkl Sayes, Christie M. verfasserin aut The link between delivered aerosol dose and inflammatory responses: Exposing a lung Cell Co-Culture system to selected Allergens and irritants 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. Aerosol engineering Elsevier Respiratory cell culture Elsevier Air-liquid interface Elsevier In vitro exposure System Elsevier Dosimetry Elsevier Singal, Madhuri oth Enthalten in Elsevier Rincón, Luis ELSEVIER The information content of the conditional pair probability 2015 an international journal Amsterdam (DE-627)ELV023917989 volume:151 year:2021 pages:0 https://doi.org/10.1016/j.jaerosci.2020.105677 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_72 58.10 Verfahrenstechnik: Allgemeines VZ AR 151 2021 0 |
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link between delivered aerosol dose and inflammatory responses: exposing a lung cell co-culture system to selected allergens and irritants |
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The link between delivered aerosol dose and inflammatory responses: Exposing a lung Cell Co-Culture system to selected Allergens and irritants |
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Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. |
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Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. |
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Traditional in vivo inhalation studies to inform hazard identification and risk assessment are time-intensive and financially expensive. Cell culture models combined with aerosol exposure systems have shown promise as surrogates in screening assays when multiple substances require preliminary information on hazard potential associated with aerosolized materials. This paper presents two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. Both apparatuses have been demonstrated for aerosol delivery capable of exposing cells in culture at the air-liquid interface. The settling chamber was constructed with simple design specifications, but exhibited a low deposition efficiency. The gentle impactor required a more sophisticated design based on aerosol dynamics of target size for the droplet delivery, but ultimately produced 64.25-fold greater deposition efficiency than the settling chamber. This led to a shorter exposure duration for the same dosage. It also enabled consistent exposure to the same sized aerosols. From these results, the gentle impactor shows promise as a high-throughput exposure tool that could be used in conjunction with cell-based co-culture models. Using a triple cell co-culture model of epithelial, dendritic, and macrophage cell-types, the induced inflammatory markers produced a more definitive dose-response for known irritants and positive indication for sensitizers. The materials tested include toluene-2,4-diisocyanate and trimellitic anhydride as known respiratory sensitizers; silica as a known respiratory irritant; and d-limonene as a known dermal sensitizer. Conversely, when using single cell monolayers, no significant different responses in inflammatory biomarkers was observed, suggesting cellular cross-talk is important. Evaluation of the ability to deliver aerosolized substances consistently and measure cytotoxicity and inflammatory endpoints is possible and demonstrates a potential high throughput screening tool. |
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