Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines

Complicated viral interactions make viral co-infections more difficult to treat. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) causes huge economic losses to the salmonid industry in China. To reveal the impact of IHNV on IPNV during c...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Xu, Liming [verfasserIn] Zhao, Jingzhuang [verfasserIn] Liu, Miao [verfasserIn] Ren, Guangming [verfasserIn] Han, Shi Cheng [verfasserIn] Shao, Yizhi [verfasserIn] Cao, Yongsheng [verfasserIn] Liu, Hongbai [verfasserIn] Lu, Tongyan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Co-infection Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Synergistic effect

Übergeordnetes Werk:	Enthalten in: Aquaculture - Amsterdam [u.a.] : Elsevier Science, 1972, 531
Übergeordnetes Werk:	volume:531

DOI / URN:	10.1016/j.aquaculture.2020.735898

Katalog-ID:	ELV052154106

Internformat


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245	1	0	\|a Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines
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520			\|a Complicated viral interactions make viral co-infections more difficult to treat. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) causes huge economic losses to the salmonid industry in China. To reveal the impact of IHNV on IPNV during co-infection, Chinook salmon embryo (CHSE-214) cells were inoculated with IHNV prior to or after IPNV at different time intervals, and propagation of IPNV was determined at the RNA, protein, and virion levels by real-time quantitative polymerase chain reaction (RT-qPCR), an immunofluorescence antibody test (IFAT), flow cytometry, and virus titration. Taken together, the results showed that IPNV multiplication was enhanced in all of the co-infection groups compared with the single IPNV infection group. This enhancement became stronger with an increasing infection time interval irrespective of the infection order, and the highest IPNV multiplication level was observed when IHNV was inoculated 12 h after IPNV (P12H). Compared with the single IPNV infection group, the IPNV RNA level in the P12H group was at least 10-fold higher, the proportions of IPNV-infected cells determined by IFAT and flow cytometry were approximately 2-fold higher, and the viral titer was about 40-fold higher. A significant difference was detected between each group in which IHNV was inoculated after IPNV. These findings suggested that a synergistic effect was induced by IHNV over IPNV, and IPNV multiplication was enhanced during co-infection in CHSE-214 cells. This enhancement was dependent on the infection time interval but not the infection order and the synergistic effect became stronger with increasing infection time interval within the study period.
650		4	\|a Co-infection
650		4	\|a Infectious hematopoietic necrosis virus
650		4	\|a Infectious pancreatic necrosis virus
650		4	\|a Synergistic effect
700	1		\|a Zhao, Jingzhuang \|e verfasserin \|4 aut
700	1		\|a Liu, Miao \|e verfasserin \|4 aut
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700	1		\|a Han, Shi Cheng \|e verfasserin \|4 aut
700	1		\|a Shao, Yizhi \|e verfasserin \|4 aut
700	1		\|a Cao, Yongsheng \|e verfasserin \|4 aut
700	1		\|a Liu, Hongbai \|e verfasserin \|4 aut
700	1		\|a Lu, Tongyan \|e verfasserin \|4 aut
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936	b	k	\|a 48.68 \|j Aquakultur \|q VZ
951			\|a AR
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author_variant	l x lx j z jz m l ml g r gr s c h sc sch y s ys y c yc h l hl t l tl
matchkey_str	xulimingzhaojingzhuangliumiaorenguangmin:2020----:netoseaooeincoivrsnacdnetosacetcerssiumlilctodrncif
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allfields	10.1016/j.aquaculture.2020.735898 doi (DE-627)ELV052154106 (ELSEVIER)S0044-8486(20)30800-0 DE-627 ger DE-627 rda eng 570 550 VZ BIODIV DE-30 fid 48.68 bkl Xu, Liming verfasserin aut Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Complicated viral interactions make viral co-infections more difficult to treat. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) causes huge economic losses to the salmonid industry in China. To reveal the impact of IHNV on IPNV during co-infection, Chinook salmon embryo (CHSE-214) cells were inoculated with IHNV prior to or after IPNV at different time intervals, and propagation of IPNV was determined at the RNA, protein, and virion levels by real-time quantitative polymerase chain reaction (RT-qPCR), an immunofluorescence antibody test (IFAT), flow cytometry, and virus titration. Taken together, the results showed that IPNV multiplication was enhanced in all of the co-infection groups compared with the single IPNV infection group. This enhancement became stronger with an increasing infection time interval irrespective of the infection order, and the highest IPNV multiplication level was observed when IHNV was inoculated 12 h after IPNV (P12H). Compared with the single IPNV infection group, the IPNV RNA level in the P12H group was at least 10-fold higher, the proportions of IPNV-infected cells determined by IFAT and flow cytometry were approximately 2-fold higher, and the viral titer was about 40-fold higher. A significant difference was detected between each group in which IHNV was inoculated after IPNV. These findings suggested that a synergistic effect was induced by IHNV over IPNV, and IPNV multiplication was enhanced during co-infection in CHSE-214 cells. This enhancement was dependent on the infection time interval but not the infection order and the synergistic effect became stronger with increasing infection time interval within the study period. Co-infection Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Synergistic effect Zhao, Jingzhuang verfasserin aut Liu, Miao verfasserin aut Ren, Guangming verfasserin aut Han, Shi Cheng verfasserin aut Shao, Yizhi verfasserin aut Cao, Yongsheng verfasserin aut Liu, Hongbai verfasserin aut Lu, Tongyan verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 531 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:531 GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur VZ AR 531
spelling	10.1016/j.aquaculture.2020.735898 doi (DE-627)ELV052154106 (ELSEVIER)S0044-8486(20)30800-0 DE-627 ger DE-627 rda eng 570 550 VZ BIODIV DE-30 fid 48.68 bkl Xu, Liming verfasserin aut Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Complicated viral interactions make viral co-infections more difficult to treat. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) causes huge economic losses to the salmonid industry in China. To reveal the impact of IHNV on IPNV during co-infection, Chinook salmon embryo (CHSE-214) cells were inoculated with IHNV prior to or after IPNV at different time intervals, and propagation of IPNV was determined at the RNA, protein, and virion levels by real-time quantitative polymerase chain reaction (RT-qPCR), an immunofluorescence antibody test (IFAT), flow cytometry, and virus titration. Taken together, the results showed that IPNV multiplication was enhanced in all of the co-infection groups compared with the single IPNV infection group. This enhancement became stronger with an increasing infection time interval irrespective of the infection order, and the highest IPNV multiplication level was observed when IHNV was inoculated 12 h after IPNV (P12H). Compared with the single IPNV infection group, the IPNV RNA level in the P12H group was at least 10-fold higher, the proportions of IPNV-infected cells determined by IFAT and flow cytometry were approximately 2-fold higher, and the viral titer was about 40-fold higher. A significant difference was detected between each group in which IHNV was inoculated after IPNV. These findings suggested that a synergistic effect was induced by IHNV over IPNV, and IPNV multiplication was enhanced during co-infection in CHSE-214 cells. This enhancement was dependent on the infection time interval but not the infection order and the synergistic effect became stronger with increasing infection time interval within the study period. Co-infection Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Synergistic effect Zhao, Jingzhuang verfasserin aut Liu, Miao verfasserin aut Ren, Guangming verfasserin aut Han, Shi Cheng verfasserin aut Shao, Yizhi verfasserin aut Cao, Yongsheng verfasserin aut Liu, Hongbai verfasserin aut Lu, Tongyan verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 531 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:531 GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur VZ AR 531
allfields_unstemmed	10.1016/j.aquaculture.2020.735898 doi (DE-627)ELV052154106 (ELSEVIER)S0044-8486(20)30800-0 DE-627 ger DE-627 rda eng 570 550 VZ BIODIV DE-30 fid 48.68 bkl Xu, Liming verfasserin aut Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Complicated viral interactions make viral co-infections more difficult to treat. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) causes huge economic losses to the salmonid industry in China. To reveal the impact of IHNV on IPNV during co-infection, Chinook salmon embryo (CHSE-214) cells were inoculated with IHNV prior to or after IPNV at different time intervals, and propagation of IPNV was determined at the RNA, protein, and virion levels by real-time quantitative polymerase chain reaction (RT-qPCR), an immunofluorescence antibody test (IFAT), flow cytometry, and virus titration. Taken together, the results showed that IPNV multiplication was enhanced in all of the co-infection groups compared with the single IPNV infection group. This enhancement became stronger with an increasing infection time interval irrespective of the infection order, and the highest IPNV multiplication level was observed when IHNV was inoculated 12 h after IPNV (P12H). Compared with the single IPNV infection group, the IPNV RNA level in the P12H group was at least 10-fold higher, the proportions of IPNV-infected cells determined by IFAT and flow cytometry were approximately 2-fold higher, and the viral titer was about 40-fold higher. A significant difference was detected between each group in which IHNV was inoculated after IPNV. These findings suggested that a synergistic effect was induced by IHNV over IPNV, and IPNV multiplication was enhanced during co-infection in CHSE-214 cells. This enhancement was dependent on the infection time interval but not the infection order and the synergistic effect became stronger with increasing infection time interval within the study period. Co-infection Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Synergistic effect Zhao, Jingzhuang verfasserin aut Liu, Miao verfasserin aut Ren, Guangming verfasserin aut Han, Shi Cheng verfasserin aut Shao, Yizhi verfasserin aut Cao, Yongsheng verfasserin aut Liu, Hongbai verfasserin aut Lu, Tongyan verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 531 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:531 GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur VZ AR 531
allfieldsGer	10.1016/j.aquaculture.2020.735898 doi (DE-627)ELV052154106 (ELSEVIER)S0044-8486(20)30800-0 DE-627 ger DE-627 rda eng 570 550 VZ BIODIV DE-30 fid 48.68 bkl Xu, Liming verfasserin aut Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Complicated viral interactions make viral co-infections more difficult to treat. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) causes huge economic losses to the salmonid industry in China. To reveal the impact of IHNV on IPNV during co-infection, Chinook salmon embryo (CHSE-214) cells were inoculated with IHNV prior to or after IPNV at different time intervals, and propagation of IPNV was determined at the RNA, protein, and virion levels by real-time quantitative polymerase chain reaction (RT-qPCR), an immunofluorescence antibody test (IFAT), flow cytometry, and virus titration. Taken together, the results showed that IPNV multiplication was enhanced in all of the co-infection groups compared with the single IPNV infection group. This enhancement became stronger with an increasing infection time interval irrespective of the infection order, and the highest IPNV multiplication level was observed when IHNV was inoculated 12 h after IPNV (P12H). Compared with the single IPNV infection group, the IPNV RNA level in the P12H group was at least 10-fold higher, the proportions of IPNV-infected cells determined by IFAT and flow cytometry were approximately 2-fold higher, and the viral titer was about 40-fold higher. A significant difference was detected between each group in which IHNV was inoculated after IPNV. These findings suggested that a synergistic effect was induced by IHNV over IPNV, and IPNV multiplication was enhanced during co-infection in CHSE-214 cells. This enhancement was dependent on the infection time interval but not the infection order and the synergistic effect became stronger with increasing infection time interval within the study period. Co-infection Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Synergistic effect Zhao, Jingzhuang verfasserin aut Liu, Miao verfasserin aut Ren, Guangming verfasserin aut Han, Shi Cheng verfasserin aut Shao, Yizhi verfasserin aut Cao, Yongsheng verfasserin aut Liu, Hongbai verfasserin aut Lu, Tongyan verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 531 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:531 GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur VZ AR 531
allfieldsSound	10.1016/j.aquaculture.2020.735898 doi (DE-627)ELV052154106 (ELSEVIER)S0044-8486(20)30800-0 DE-627 ger DE-627 rda eng 570 550 VZ BIODIV DE-30 fid 48.68 bkl Xu, Liming verfasserin aut Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Complicated viral interactions make viral co-infections more difficult to treat. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) causes huge economic losses to the salmonid industry in China. To reveal the impact of IHNV on IPNV during co-infection, Chinook salmon embryo (CHSE-214) cells were inoculated with IHNV prior to or after IPNV at different time intervals, and propagation of IPNV was determined at the RNA, protein, and virion levels by real-time quantitative polymerase chain reaction (RT-qPCR), an immunofluorescence antibody test (IFAT), flow cytometry, and virus titration. Taken together, the results showed that IPNV multiplication was enhanced in all of the co-infection groups compared with the single IPNV infection group. This enhancement became stronger with an increasing infection time interval irrespective of the infection order, and the highest IPNV multiplication level was observed when IHNV was inoculated 12 h after IPNV (P12H). Compared with the single IPNV infection group, the IPNV RNA level in the P12H group was at least 10-fold higher, the proportions of IPNV-infected cells determined by IFAT and flow cytometry were approximately 2-fold higher, and the viral titer was about 40-fold higher. A significant difference was detected between each group in which IHNV was inoculated after IPNV. These findings suggested that a synergistic effect was induced by IHNV over IPNV, and IPNV multiplication was enhanced during co-infection in CHSE-214 cells. This enhancement was dependent on the infection time interval but not the infection order and the synergistic effect became stronger with increasing infection time interval within the study period. Co-infection Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Synergistic effect Zhao, Jingzhuang verfasserin aut Liu, Miao verfasserin aut Ren, Guangming verfasserin aut Han, Shi Cheng verfasserin aut Shao, Yizhi verfasserin aut Cao, Yongsheng verfasserin aut Liu, Hongbai verfasserin aut Lu, Tongyan verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 531 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:531 GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur VZ AR 531
language	English
source	Enthalten in Aquaculture 531 volume:531
sourceStr	Enthalten in Aquaculture 531 volume:531
format_phy_str_mv	Article
bklname	Aquakultur
institution	findex.gbv.de
topic_facet	Co-infection Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Synergistic effect
dewey-raw	570
isfreeaccess_bool	false
container_title	Aquaculture
authorswithroles_txt_mv	Xu, Liming @@aut@@ Zhao, Jingzhuang @@aut@@ Liu, Miao @@aut@@ Ren, Guangming @@aut@@ Han, Shi Cheng @@aut@@ Shao, Yizhi @@aut@@ Cao, Yongsheng @@aut@@ Liu, Hongbai @@aut@@ Lu, Tongyan @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	306314002
dewey-sort	3570
id	ELV052154106
language_de	englisch
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author	Xu, Liming
spellingShingle	Xu, Liming ddc 570 fid BIODIV bkl 48.68 misc Co-infection misc Infectious hematopoietic necrosis virus misc Infectious pancreatic necrosis virus misc Synergistic effect Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines
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topic_title	570 550 VZ BIODIV DE-30 fid 48.68 bkl Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines Co-infection Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Synergistic effect
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topic_browse	ddc 570 fid BIODIV bkl 48.68 misc Co-infection misc Infectious hematopoietic necrosis virus misc Infectious pancreatic necrosis virus misc Synergistic effect
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title	Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines
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title_full	Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines
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author_browse	Xu, Liming Zhao, Jingzhuang Liu, Miao Ren, Guangming Han, Shi Cheng Shao, Yizhi Cao, Yongsheng Liu, Hongbai Lu, Tongyan
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title_sort	infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in chinook salmon embryo cell lines
title_auth	Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines
abstract	Complicated viral interactions make viral co-infections more difficult to treat. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) causes huge economic losses to the salmonid industry in China. To reveal the impact of IHNV on IPNV during co-infection, Chinook salmon embryo (CHSE-214) cells were inoculated with IHNV prior to or after IPNV at different time intervals, and propagation of IPNV was determined at the RNA, protein, and virion levels by real-time quantitative polymerase chain reaction (RT-qPCR), an immunofluorescence antibody test (IFAT), flow cytometry, and virus titration. Taken together, the results showed that IPNV multiplication was enhanced in all of the co-infection groups compared with the single IPNV infection group. This enhancement became stronger with an increasing infection time interval irrespective of the infection order, and the highest IPNV multiplication level was observed when IHNV was inoculated 12 h after IPNV (P12H). Compared with the single IPNV infection group, the IPNV RNA level in the P12H group was at least 10-fold higher, the proportions of IPNV-infected cells determined by IFAT and flow cytometry were approximately 2-fold higher, and the viral titer was about 40-fold higher. A significant difference was detected between each group in which IHNV was inoculated after IPNV. These findings suggested that a synergistic effect was induced by IHNV over IPNV, and IPNV multiplication was enhanced during co-infection in CHSE-214 cells. This enhancement was dependent on the infection time interval but not the infection order and the synergistic effect became stronger with increasing infection time interval within the study period.
abstractGer	Complicated viral interactions make viral co-infections more difficult to treat. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) causes huge economic losses to the salmonid industry in China. To reveal the impact of IHNV on IPNV during co-infection, Chinook salmon embryo (CHSE-214) cells were inoculated with IHNV prior to or after IPNV at different time intervals, and propagation of IPNV was determined at the RNA, protein, and virion levels by real-time quantitative polymerase chain reaction (RT-qPCR), an immunofluorescence antibody test (IFAT), flow cytometry, and virus titration. Taken together, the results showed that IPNV multiplication was enhanced in all of the co-infection groups compared with the single IPNV infection group. This enhancement became stronger with an increasing infection time interval irrespective of the infection order, and the highest IPNV multiplication level was observed when IHNV was inoculated 12 h after IPNV (P12H). Compared with the single IPNV infection group, the IPNV RNA level in the P12H group was at least 10-fold higher, the proportions of IPNV-infected cells determined by IFAT and flow cytometry were approximately 2-fold higher, and the viral titer was about 40-fold higher. A significant difference was detected between each group in which IHNV was inoculated after IPNV. These findings suggested that a synergistic effect was induced by IHNV over IPNV, and IPNV multiplication was enhanced during co-infection in CHSE-214 cells. This enhancement was dependent on the infection time interval but not the infection order and the synergistic effect became stronger with increasing infection time interval within the study period.
abstract_unstemmed	Complicated viral interactions make viral co-infections more difficult to treat. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) causes huge economic losses to the salmonid industry in China. To reveal the impact of IHNV on IPNV during co-infection, Chinook salmon embryo (CHSE-214) cells were inoculated with IHNV prior to or after IPNV at different time intervals, and propagation of IPNV was determined at the RNA, protein, and virion levels by real-time quantitative polymerase chain reaction (RT-qPCR), an immunofluorescence antibody test (IFAT), flow cytometry, and virus titration. Taken together, the results showed that IPNV multiplication was enhanced in all of the co-infection groups compared with the single IPNV infection group. This enhancement became stronger with an increasing infection time interval irrespective of the infection order, and the highest IPNV multiplication level was observed when IHNV was inoculated 12 h after IPNV (P12H). Compared with the single IPNV infection group, the IPNV RNA level in the P12H group was at least 10-fold higher, the proportions of IPNV-infected cells determined by IFAT and flow cytometry were approximately 2-fold higher, and the viral titer was about 40-fold higher. A significant difference was detected between each group in which IHNV was inoculated after IPNV. These findings suggested that a synergistic effect was induced by IHNV over IPNV, and IPNV multiplication was enhanced during co-infection in CHSE-214 cells. This enhancement was dependent on the infection time interval but not the infection order and the synergistic effect became stronger with increasing infection time interval within the study period.
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title_short	Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines
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author2	Zhao, Jingzhuang Liu, Miao Ren, Guangming Han, Shi Cheng Shao, Yizhi Cao, Yongsheng Liu, Hongbai Lu, Tongyan
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up_date	2024-07-06T22:14:40.007Z
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Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines

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