Super-resolution imaging of flat-mounted whole mouse cornea
Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatib...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Cai, Zhen [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021transfer abstract |
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| Schlagwörter: |
Super-resolution optical microscopy |
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| Übergeordnetes Werk: |
Enthalten in: Comparison of several interpolation methods to reconstruct field data in the vicinity of a finite element immersed boundary - Billo, Georis ELSEVIER, 2022, official journal of the ISER, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:205 ; year:2021 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.exer.2021.108499 |
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| 520 | |a Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. | ||
| 520 | |a Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. | ||
| 650 | 7 | |a Super-resolution optical microscopy |2 Elsevier | |
| 650 | 7 | |a Single-molecule localization microscopy |2 Elsevier | |
| 650 | 7 | |a Corneal endothelium |2 Elsevier | |
| 700 | 1 | |a Zhang, Yang |4 oth | |
| 700 | 1 | |a Zhang, Zheyuan |4 oth | |
| 700 | 1 | |a Song, Ki-Hee |4 oth | |
| 700 | 1 | |a Beckmann, Lisa |4 oth | |
| 700 | 1 | |a Djalilian, Ali |4 oth | |
| 700 | 1 | |a Sun, Cheng |4 oth | |
| 700 | 1 | |a Zhang, Hao F. |4 oth | |
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10.1016/j.exer.2021.108499 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001355.pica (DE-627)ELV053630246 (ELSEVIER)S0014-4835(21)00064-6 DE-627 ger DE-627 rakwb eng 510 004 VZ 31.80 bkl 54.80 bkl Cai, Zhen verfasserin aut Super-resolution imaging of flat-mounted whole mouse cornea 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. Super-resolution optical microscopy Elsevier Single-molecule localization microscopy Elsevier Corneal endothelium Elsevier Zhang, Yang oth Zhang, Zheyuan oth Song, Ki-Hee oth Beckmann, Lisa oth Djalilian, Ali oth Sun, Cheng oth Zhang, Hao F. oth Enthalten in Elsevier Billo, Georis ELSEVIER Comparison of several interpolation methods to reconstruct field data in the vicinity of a finite element immersed boundary 2022 official journal of the ISER Amsterdam [u.a.] (DE-627)ELV008415374 volume:205 year:2021 pages:0 https://doi.org/10.1016/j.exer.2021.108499 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-MAT 31.80 Angewandte Mathematik VZ 54.80 Angewandte Informatik VZ AR 205 2021 0 |
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10.1016/j.exer.2021.108499 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001355.pica (DE-627)ELV053630246 (ELSEVIER)S0014-4835(21)00064-6 DE-627 ger DE-627 rakwb eng 510 004 VZ 31.80 bkl 54.80 bkl Cai, Zhen verfasserin aut Super-resolution imaging of flat-mounted whole mouse cornea 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. Super-resolution optical microscopy Elsevier Single-molecule localization microscopy Elsevier Corneal endothelium Elsevier Zhang, Yang oth Zhang, Zheyuan oth Song, Ki-Hee oth Beckmann, Lisa oth Djalilian, Ali oth Sun, Cheng oth Zhang, Hao F. oth Enthalten in Elsevier Billo, Georis ELSEVIER Comparison of several interpolation methods to reconstruct field data in the vicinity of a finite element immersed boundary 2022 official journal of the ISER Amsterdam [u.a.] (DE-627)ELV008415374 volume:205 year:2021 pages:0 https://doi.org/10.1016/j.exer.2021.108499 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-MAT 31.80 Angewandte Mathematik VZ 54.80 Angewandte Informatik VZ AR 205 2021 0 |
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10.1016/j.exer.2021.108499 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001355.pica (DE-627)ELV053630246 (ELSEVIER)S0014-4835(21)00064-6 DE-627 ger DE-627 rakwb eng 510 004 VZ 31.80 bkl 54.80 bkl Cai, Zhen verfasserin aut Super-resolution imaging of flat-mounted whole mouse cornea 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. Super-resolution optical microscopy Elsevier Single-molecule localization microscopy Elsevier Corneal endothelium Elsevier Zhang, Yang oth Zhang, Zheyuan oth Song, Ki-Hee oth Beckmann, Lisa oth Djalilian, Ali oth Sun, Cheng oth Zhang, Hao F. oth Enthalten in Elsevier Billo, Georis ELSEVIER Comparison of several interpolation methods to reconstruct field data in the vicinity of a finite element immersed boundary 2022 official journal of the ISER Amsterdam [u.a.] (DE-627)ELV008415374 volume:205 year:2021 pages:0 https://doi.org/10.1016/j.exer.2021.108499 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-MAT 31.80 Angewandte Mathematik VZ 54.80 Angewandte Informatik VZ AR 205 2021 0 |
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10.1016/j.exer.2021.108499 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001355.pica (DE-627)ELV053630246 (ELSEVIER)S0014-4835(21)00064-6 DE-627 ger DE-627 rakwb eng 510 004 VZ 31.80 bkl 54.80 bkl Cai, Zhen verfasserin aut Super-resolution imaging of flat-mounted whole mouse cornea 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. Super-resolution optical microscopy Elsevier Single-molecule localization microscopy Elsevier Corneal endothelium Elsevier Zhang, Yang oth Zhang, Zheyuan oth Song, Ki-Hee oth Beckmann, Lisa oth Djalilian, Ali oth Sun, Cheng oth Zhang, Hao F. oth Enthalten in Elsevier Billo, Georis ELSEVIER Comparison of several interpolation methods to reconstruct field data in the vicinity of a finite element immersed boundary 2022 official journal of the ISER Amsterdam [u.a.] (DE-627)ELV008415374 volume:205 year:2021 pages:0 https://doi.org/10.1016/j.exer.2021.108499 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-MAT 31.80 Angewandte Mathematik VZ 54.80 Angewandte Informatik VZ AR 205 2021 0 |
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10.1016/j.exer.2021.108499 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001355.pica (DE-627)ELV053630246 (ELSEVIER)S0014-4835(21)00064-6 DE-627 ger DE-627 rakwb eng 510 004 VZ 31.80 bkl 54.80 bkl Cai, Zhen verfasserin aut Super-resolution imaging of flat-mounted whole mouse cornea 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. Super-resolution optical microscopy Elsevier Single-molecule localization microscopy Elsevier Corneal endothelium Elsevier Zhang, Yang oth Zhang, Zheyuan oth Song, Ki-Hee oth Beckmann, Lisa oth Djalilian, Ali oth Sun, Cheng oth Zhang, Hao F. oth Enthalten in Elsevier Billo, Georis ELSEVIER Comparison of several interpolation methods to reconstruct field data in the vicinity of a finite element immersed boundary 2022 official journal of the ISER Amsterdam [u.a.] (DE-627)ELV008415374 volume:205 year:2021 pages:0 https://doi.org/10.1016/j.exer.2021.108499 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-MAT 31.80 Angewandte Mathematik VZ 54.80 Angewandte Informatik VZ AR 205 2021 0 |
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Enthalten in Comparison of several interpolation methods to reconstruct field data in the vicinity of a finite element immersed boundary Amsterdam [u.a.] volume:205 year:2021 pages:0 |
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Enthalten in Comparison of several interpolation methods to reconstruct field data in the vicinity of a finite element immersed boundary Amsterdam [u.a.] volume:205 year:2021 pages:0 |
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Comparison of several interpolation methods to reconstruct field data in the vicinity of a finite element immersed boundary |
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To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. 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Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. |
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Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. |
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Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures. |
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