Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee
Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zangheri, Martina [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Neuro-Brucellosis - Gouider, R. ELSEVIER, 2015, an international journal devoted to all branches of analytical chemistry, Amsterdam |
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| Übergeordnetes Werk: |
volume:1163 ; year:2021 ; day:8 ; month:06 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.aca.2021.338515 |
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ELV054103029 |
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| 245 | 1 | 0 | |a Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee |
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| 520 | |a Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. | ||
| 520 | |a Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. | ||
| 650 | 7 | |a Lateral flow immunoassay |2 Elsevier | |
| 650 | 7 | |a Mycotoxins |2 Elsevier | |
| 650 | 7 | |a Chemiluminescence |2 Elsevier | |
| 650 | 7 | |a Food safety |2 Elsevier | |
| 650 | 7 | |a Point of need |2 Elsevier | |
| 650 | 7 | |a Ochratoxin A |2 Elsevier | |
| 650 | 7 | |a Smartphone |2 Elsevier | |
| 700 | 1 | |a Di Nardo, Fabio |4 oth | |
| 700 | 1 | |a Calabria, Donato |4 oth | |
| 700 | 1 | |a Marchegiani, Elisa |4 oth | |
| 700 | 1 | |a Anfossi, Laura |4 oth | |
| 700 | 1 | |a Guardigli, Massimo |4 oth | |
| 700 | 1 | |a Mirasoli, Mara |4 oth | |
| 700 | 1 | |a Baggiani, Claudio |4 oth | |
| 700 | 1 | |a Roda, Aldo |4 oth | |
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10.1016/j.aca.2021.338515 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001398.pica (DE-627)ELV054103029 (ELSEVIER)S0003-2670(21)00341-X DE-627 ger DE-627 rakwb eng 610 VZ 540 VZ 35.10 bkl Zangheri, Martina verfasserin aut Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. Lateral flow immunoassay Elsevier Mycotoxins Elsevier Chemiluminescence Elsevier Food safety Elsevier Point of need Elsevier Ochratoxin A Elsevier Smartphone Elsevier Di Nardo, Fabio oth Calabria, Donato oth Marchegiani, Elisa oth Anfossi, Laura oth Guardigli, Massimo oth Mirasoli, Mara oth Baggiani, Claudio oth Roda, Aldo oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:1163 year:2021 day:8 month:06 pages:0 https://doi.org/10.1016/j.aca.2021.338515 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 1163 2021 8 0608 0 |
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10.1016/j.aca.2021.338515 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001398.pica (DE-627)ELV054103029 (ELSEVIER)S0003-2670(21)00341-X DE-627 ger DE-627 rakwb eng 610 VZ 540 VZ 35.10 bkl Zangheri, Martina verfasserin aut Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. Lateral flow immunoassay Elsevier Mycotoxins Elsevier Chemiluminescence Elsevier Food safety Elsevier Point of need Elsevier Ochratoxin A Elsevier Smartphone Elsevier Di Nardo, Fabio oth Calabria, Donato oth Marchegiani, Elisa oth Anfossi, Laura oth Guardigli, Massimo oth Mirasoli, Mara oth Baggiani, Claudio oth Roda, Aldo oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:1163 year:2021 day:8 month:06 pages:0 https://doi.org/10.1016/j.aca.2021.338515 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 1163 2021 8 0608 0 |
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10.1016/j.aca.2021.338515 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001398.pica (DE-627)ELV054103029 (ELSEVIER)S0003-2670(21)00341-X DE-627 ger DE-627 rakwb eng 610 VZ 540 VZ 35.10 bkl Zangheri, Martina verfasserin aut Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. Lateral flow immunoassay Elsevier Mycotoxins Elsevier Chemiluminescence Elsevier Food safety Elsevier Point of need Elsevier Ochratoxin A Elsevier Smartphone Elsevier Di Nardo, Fabio oth Calabria, Donato oth Marchegiani, Elisa oth Anfossi, Laura oth Guardigli, Massimo oth Mirasoli, Mara oth Baggiani, Claudio oth Roda, Aldo oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:1163 year:2021 day:8 month:06 pages:0 https://doi.org/10.1016/j.aca.2021.338515 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 1163 2021 8 0608 0 |
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10.1016/j.aca.2021.338515 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001398.pica (DE-627)ELV054103029 (ELSEVIER)S0003-2670(21)00341-X DE-627 ger DE-627 rakwb eng 610 VZ 540 VZ 35.10 bkl Zangheri, Martina verfasserin aut Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. Lateral flow immunoassay Elsevier Mycotoxins Elsevier Chemiluminescence Elsevier Food safety Elsevier Point of need Elsevier Ochratoxin A Elsevier Smartphone Elsevier Di Nardo, Fabio oth Calabria, Donato oth Marchegiani, Elisa oth Anfossi, Laura oth Guardigli, Massimo oth Mirasoli, Mara oth Baggiani, Claudio oth Roda, Aldo oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:1163 year:2021 day:8 month:06 pages:0 https://doi.org/10.1016/j.aca.2021.338515 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 1163 2021 8 0608 0 |
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10.1016/j.aca.2021.338515 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001398.pica (DE-627)ELV054103029 (ELSEVIER)S0003-2670(21)00341-X DE-627 ger DE-627 rakwb eng 610 VZ 540 VZ 35.10 bkl Zangheri, Martina verfasserin aut Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. Lateral flow immunoassay Elsevier Mycotoxins Elsevier Chemiluminescence Elsevier Food safety Elsevier Point of need Elsevier Ochratoxin A Elsevier Smartphone Elsevier Di Nardo, Fabio oth Calabria, Donato oth Marchegiani, Elisa oth Anfossi, Laura oth Guardigli, Massimo oth Mirasoli, Mara oth Baggiani, Claudio oth Roda, Aldo oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:1163 year:2021 day:8 month:06 pages:0 https://doi.org/10.1016/j.aca.2021.338515 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 1163 2021 8 0608 0 |
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smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin a in wine and coffee |
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Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee |
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Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. |
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Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. |
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Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis. |
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