An inexpensive anaerobic chamber for the genetic manipulation of strictly anaerobic bacteria
Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to s...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Hong, Wei [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Design, synthesis and biological evaluation of 5-amino-4-(1H-benzoimidazol-2-yl)-phenyl-1,2-dihydro-pyrrol-3-ones as inhibitors of protein kinase FGFR1 - Gryshchenko, A.A. ELSEVIER, 2016, the official journal of the Anaerobe Society of the Americas, London |
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| Übergeordnetes Werk: |
volume:69 ; year:2021 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.anaerobe.2021.102349 |
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| 245 | 1 | 0 | |a An inexpensive anaerobic chamber for the genetic manipulation of strictly anaerobic bacteria |
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| 520 | |a Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. | ||
| 520 | |a Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. | ||
| 650 | 7 | |a Strictly anaerobic bacteria |2 Elsevier | |
| 650 | 7 | |a Cultivation |2 Elsevier | |
| 650 | 7 | |a Anaerobic chamber |2 Elsevier | |
| 650 | 7 | |a Gene manipulation |2 Elsevier | |
| 700 | 1 | |a Rao, Feng-qin |4 oth | |
| 700 | 1 | |a Zhao, Xing-xing |4 oth | |
| 700 | 1 | |a Guo, Zhen Yang |4 oth | |
| 700 | 1 | |a Chen, Yu-mei |4 oth | |
| 700 | 1 | |a Wang, Bing |4 oth | |
| 700 | 1 | |a Guan, Zhi-zhong |4 oth | |
| 700 | 1 | |a Qi, Xiao-lan |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Academic Press |a Gryshchenko, A.A. ELSEVIER |t Design, synthesis and biological evaluation of 5-amino-4-(1H-benzoimidazol-2-yl)-phenyl-1,2-dihydro-pyrrol-3-ones as inhibitors of protein kinase FGFR1 |d 2016 |d the official journal of the Anaerobe Society of the Americas |g London |w (DE-627)ELV019616112 |
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10.1016/j.anaerobe.2021.102349 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001626.pica (DE-627)ELV05439323X (ELSEVIER)S1075-9964(21)00032-9 DE-627 ger DE-627 rakwb eng 540 VZ 610 VZ 004 VZ 53.70 bkl 05.42 bkl 53.76 bkl 54.00 bkl Hong, Wei verfasserin aut An inexpensive anaerobic chamber for the genetic manipulation of strictly anaerobic bacteria 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. Strictly anaerobic bacteria Elsevier Cultivation Elsevier Anaerobic chamber Elsevier Gene manipulation Elsevier Rao, Feng-qin oth Zhao, Xing-xing oth Guo, Zhen Yang oth Chen, Yu-mei oth Wang, Bing oth Guan, Zhi-zhong oth Qi, Xiao-lan oth Enthalten in Academic Press Gryshchenko, A.A. ELSEVIER Design, synthesis and biological evaluation of 5-amino-4-(1H-benzoimidazol-2-yl)-phenyl-1,2-dihydro-pyrrol-3-ones as inhibitors of protein kinase FGFR1 2016 the official journal of the Anaerobe Society of the Americas London (DE-627)ELV019616112 volume:69 year:2021 pages:0 https://doi.org/10.1016/j.anaerobe.2021.102349 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_70 53.70 Nachrichtentechnik Kommunikationstechnik: Allgemeines VZ 05.42 Telekommunikation VZ 53.76 Kommunikationsdienste Fernmeldetechnik VZ 54.00 Informatik: Allgemeines VZ AR 69 2021 0 |
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10.1016/j.anaerobe.2021.102349 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001626.pica (DE-627)ELV05439323X (ELSEVIER)S1075-9964(21)00032-9 DE-627 ger DE-627 rakwb eng 540 VZ 610 VZ 004 VZ 53.70 bkl 05.42 bkl 53.76 bkl 54.00 bkl Hong, Wei verfasserin aut An inexpensive anaerobic chamber for the genetic manipulation of strictly anaerobic bacteria 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. Strictly anaerobic bacteria Elsevier Cultivation Elsevier Anaerobic chamber Elsevier Gene manipulation Elsevier Rao, Feng-qin oth Zhao, Xing-xing oth Guo, Zhen Yang oth Chen, Yu-mei oth Wang, Bing oth Guan, Zhi-zhong oth Qi, Xiao-lan oth Enthalten in Academic Press Gryshchenko, A.A. ELSEVIER Design, synthesis and biological evaluation of 5-amino-4-(1H-benzoimidazol-2-yl)-phenyl-1,2-dihydro-pyrrol-3-ones as inhibitors of protein kinase FGFR1 2016 the official journal of the Anaerobe Society of the Americas London (DE-627)ELV019616112 volume:69 year:2021 pages:0 https://doi.org/10.1016/j.anaerobe.2021.102349 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_70 53.70 Nachrichtentechnik Kommunikationstechnik: Allgemeines VZ 05.42 Telekommunikation VZ 53.76 Kommunikationsdienste Fernmeldetechnik VZ 54.00 Informatik: Allgemeines VZ AR 69 2021 0 |
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10.1016/j.anaerobe.2021.102349 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001626.pica (DE-627)ELV05439323X (ELSEVIER)S1075-9964(21)00032-9 DE-627 ger DE-627 rakwb eng 540 VZ 610 VZ 004 VZ 53.70 bkl 05.42 bkl 53.76 bkl 54.00 bkl Hong, Wei verfasserin aut An inexpensive anaerobic chamber for the genetic manipulation of strictly anaerobic bacteria 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. Strictly anaerobic bacteria Elsevier Cultivation Elsevier Anaerobic chamber Elsevier Gene manipulation Elsevier Rao, Feng-qin oth Zhao, Xing-xing oth Guo, Zhen Yang oth Chen, Yu-mei oth Wang, Bing oth Guan, Zhi-zhong oth Qi, Xiao-lan oth Enthalten in Academic Press Gryshchenko, A.A. ELSEVIER Design, synthesis and biological evaluation of 5-amino-4-(1H-benzoimidazol-2-yl)-phenyl-1,2-dihydro-pyrrol-3-ones as inhibitors of protein kinase FGFR1 2016 the official journal of the Anaerobe Society of the Americas London (DE-627)ELV019616112 volume:69 year:2021 pages:0 https://doi.org/10.1016/j.anaerobe.2021.102349 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_70 53.70 Nachrichtentechnik Kommunikationstechnik: Allgemeines VZ 05.42 Telekommunikation VZ 53.76 Kommunikationsdienste Fernmeldetechnik VZ 54.00 Informatik: Allgemeines VZ AR 69 2021 0 |
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10.1016/j.anaerobe.2021.102349 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001626.pica (DE-627)ELV05439323X (ELSEVIER)S1075-9964(21)00032-9 DE-627 ger DE-627 rakwb eng 540 VZ 610 VZ 004 VZ 53.70 bkl 05.42 bkl 53.76 bkl 54.00 bkl Hong, Wei verfasserin aut An inexpensive anaerobic chamber for the genetic manipulation of strictly anaerobic bacteria 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. Strictly anaerobic bacteria Elsevier Cultivation Elsevier Anaerobic chamber Elsevier Gene manipulation Elsevier Rao, Feng-qin oth Zhao, Xing-xing oth Guo, Zhen Yang oth Chen, Yu-mei oth Wang, Bing oth Guan, Zhi-zhong oth Qi, Xiao-lan oth Enthalten in Academic Press Gryshchenko, A.A. ELSEVIER Design, synthesis and biological evaluation of 5-amino-4-(1H-benzoimidazol-2-yl)-phenyl-1,2-dihydro-pyrrol-3-ones as inhibitors of protein kinase FGFR1 2016 the official journal of the Anaerobe Society of the Americas London (DE-627)ELV019616112 volume:69 year:2021 pages:0 https://doi.org/10.1016/j.anaerobe.2021.102349 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_70 53.70 Nachrichtentechnik Kommunikationstechnik: Allgemeines VZ 05.42 Telekommunikation VZ 53.76 Kommunikationsdienste Fernmeldetechnik VZ 54.00 Informatik: Allgemeines VZ AR 69 2021 0 |
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10.1016/j.anaerobe.2021.102349 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001626.pica (DE-627)ELV05439323X (ELSEVIER)S1075-9964(21)00032-9 DE-627 ger DE-627 rakwb eng 540 VZ 610 VZ 004 VZ 53.70 bkl 05.42 bkl 53.76 bkl 54.00 bkl Hong, Wei verfasserin aut An inexpensive anaerobic chamber for the genetic manipulation of strictly anaerobic bacteria 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. Strictly anaerobic bacteria Elsevier Cultivation Elsevier Anaerobic chamber Elsevier Gene manipulation Elsevier Rao, Feng-qin oth Zhao, Xing-xing oth Guo, Zhen Yang oth Chen, Yu-mei oth Wang, Bing oth Guan, Zhi-zhong oth Qi, Xiao-lan oth Enthalten in Academic Press Gryshchenko, A.A. ELSEVIER Design, synthesis and biological evaluation of 5-amino-4-(1H-benzoimidazol-2-yl)-phenyl-1,2-dihydro-pyrrol-3-ones as inhibitors of protein kinase FGFR1 2016 the official journal of the Anaerobe Society of the Americas London (DE-627)ELV019616112 volume:69 year:2021 pages:0 https://doi.org/10.1016/j.anaerobe.2021.102349 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_70 53.70 Nachrichtentechnik Kommunikationstechnik: Allgemeines VZ 05.42 Telekommunikation VZ 53.76 Kommunikationsdienste Fernmeldetechnik VZ 54.00 Informatik: Allgemeines VZ AR 69 2021 0 |
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Enthalten in Design, synthesis and biological evaluation of 5-amino-4-(1H-benzoimidazol-2-yl)-phenyl-1,2-dihydro-pyrrol-3-ones as inhibitors of protein kinase FGFR1 London volume:69 year:2021 pages:0 |
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Enthalten in Design, synthesis and biological evaluation of 5-amino-4-(1H-benzoimidazol-2-yl)-phenyl-1,2-dihydro-pyrrol-3-ones as inhibitors of protein kinase FGFR1 London volume:69 year:2021 pages:0 |
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an inexpensive anaerobic chamber for the genetic manipulation of strictly anaerobic bacteria |
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An inexpensive anaerobic chamber for the genetic manipulation of strictly anaerobic bacteria |
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Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. |
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Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. |
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Strictly anaerobic bacteria are important to both human health and industrial usage. These bacteria are sensitive to oxygen, therefore, it is preferable to manipulate these microbes in an anaerobic chamber. However, commercial anaerobic chambers (CACs) are expensive, making them less accessible to scientists with a limited budget, especially to those in developing countries. The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. The anaerobic chamber described in this study will promote the research of anaerobes in developing counties and scientists who have limited research budgets. |
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The high price of commercial chambers has hindered, at least partially, the progress of research on anaerobes in developing countries. In the research presented here, we developed an inexpensive and reliable anaerobic chamber and successfully achieved routine maintenance of eleven strictly anaerobic bacterial strains. Furthermore, genetic manipulation examples have been set for both C lostridioides difficile 630 and C lostridium beijerinckii NCIMB 8052 strains to validate that the chamber could applied to advanced genetic engineering of strictly anaerobes. C. difficile and C. bei je rinckii were both genetically manipulated in this chamber, showing it’s utility for the genetic engineering of anaerobes. Most importantly, the anaerobic chamber was 76% - 88% less expensive than a CACs and has similar functionality with regards to the cultivation and manipulation of strictly anaerobic bacteria. 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ELSEVIER</subfield><subfield code="t">Design, synthesis and biological evaluation of 5-amino-4-(1H-benzoimidazol-2-yl)-phenyl-1,2-dihydro-pyrrol-3-ones as inhibitors of protein kinase FGFR1</subfield><subfield code="d">2016</subfield><subfield code="d">the official journal of the Anaerobe Society of the Americas</subfield><subfield code="g">London</subfield><subfield code="w">(DE-627)ELV019616112</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:69</subfield><subfield code="g">year:2021</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.anaerobe.2021.102349</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">53.70</subfield><subfield code="j">Nachrichtentechnik</subfield><subfield code="j">Kommunikationstechnik: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">05.42</subfield><subfield code="j">Telekommunikation</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">53.76</subfield><subfield code="j">Kommunikationsdienste</subfield><subfield code="j">Fernmeldetechnik</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">54.00</subfield><subfield code="j">Informatik: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">69</subfield><subfield code="j">2021</subfield><subfield code="h">0</subfield></datafield></record></collection>
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