Cu-mediated NIR photoinduced polymerization for highly sensitive electrochemical nucleic acid detection
An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polyme...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Liu, Qianrui [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery - Borgès Da Silva, Virginie ELSEVIER, 2018, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:349 ; year:2021 ; day:15 ; month:12 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.snb.2021.130797 |
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| 245 | 1 | 0 | |a Cu-mediated NIR photoinduced polymerization for highly sensitive electrochemical nucleic acid detection |
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| 520 | |a An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. | ||
| 520 | |a An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. | ||
| 650 | 7 | |a Cu2(OH)PO4 |2 Elsevier | |
| 650 | 7 | |a Electrochemical biosensor |2 Elsevier | |
| 650 | 7 | |a Near-infrared light |2 Elsevier | |
| 650 | 7 | |a Nucleic acid |2 Elsevier | |
| 650 | 7 | |a PhotoATRP |2 Elsevier | |
| 700 | 1 | |a Xie, Huifang |4 oth | |
| 700 | 1 | |a Kong, Jinming |4 oth | |
| 700 | 1 | |a Zhang, Xueji |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Borgès Da Silva, Virginie ELSEVIER |t Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery |d 2018 |g Amsterdam [u.a.] |w (DE-627)ELV001079875 |
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10.1016/j.snb.2021.130797 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001570.pica (DE-627)ELV055568165 (ELSEVIER)S0925-4005(21)01365-4 DE-627 ger DE-627 rakwb eng 610 VZ 44.91 bkl Liu, Qianrui verfasserin aut Cu-mediated NIR photoinduced polymerization for highly sensitive electrochemical nucleic acid detection 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. Cu2(OH)PO4 Elsevier Electrochemical biosensor Elsevier Near-infrared light Elsevier Nucleic acid Elsevier PhotoATRP Elsevier Xie, Huifang oth Kong, Jinming oth Zhang, Xueji oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:349 year:2021 day:15 month:12 pages:0 https://doi.org/10.1016/j.snb.2021.130797 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 349 2021 15 1215 0 |
| spelling |
10.1016/j.snb.2021.130797 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001570.pica (DE-627)ELV055568165 (ELSEVIER)S0925-4005(21)01365-4 DE-627 ger DE-627 rakwb eng 610 VZ 44.91 bkl Liu, Qianrui verfasserin aut Cu-mediated NIR photoinduced polymerization for highly sensitive electrochemical nucleic acid detection 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. Cu2(OH)PO4 Elsevier Electrochemical biosensor Elsevier Near-infrared light Elsevier Nucleic acid Elsevier PhotoATRP Elsevier Xie, Huifang oth Kong, Jinming oth Zhang, Xueji oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:349 year:2021 day:15 month:12 pages:0 https://doi.org/10.1016/j.snb.2021.130797 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 349 2021 15 1215 0 |
| allfields_unstemmed |
10.1016/j.snb.2021.130797 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001570.pica (DE-627)ELV055568165 (ELSEVIER)S0925-4005(21)01365-4 DE-627 ger DE-627 rakwb eng 610 VZ 44.91 bkl Liu, Qianrui verfasserin aut Cu-mediated NIR photoinduced polymerization for highly sensitive electrochemical nucleic acid detection 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. Cu2(OH)PO4 Elsevier Electrochemical biosensor Elsevier Near-infrared light Elsevier Nucleic acid Elsevier PhotoATRP Elsevier Xie, Huifang oth Kong, Jinming oth Zhang, Xueji oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:349 year:2021 day:15 month:12 pages:0 https://doi.org/10.1016/j.snb.2021.130797 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 349 2021 15 1215 0 |
| allfieldsGer |
10.1016/j.snb.2021.130797 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001570.pica (DE-627)ELV055568165 (ELSEVIER)S0925-4005(21)01365-4 DE-627 ger DE-627 rakwb eng 610 VZ 44.91 bkl Liu, Qianrui verfasserin aut Cu-mediated NIR photoinduced polymerization for highly sensitive electrochemical nucleic acid detection 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. Cu2(OH)PO4 Elsevier Electrochemical biosensor Elsevier Near-infrared light Elsevier Nucleic acid Elsevier PhotoATRP Elsevier Xie, Huifang oth Kong, Jinming oth Zhang, Xueji oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:349 year:2021 day:15 month:12 pages:0 https://doi.org/10.1016/j.snb.2021.130797 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 349 2021 15 1215 0 |
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10.1016/j.snb.2021.130797 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001570.pica (DE-627)ELV055568165 (ELSEVIER)S0925-4005(21)01365-4 DE-627 ger DE-627 rakwb eng 610 VZ 44.91 bkl Liu, Qianrui verfasserin aut Cu-mediated NIR photoinduced polymerization for highly sensitive electrochemical nucleic acid detection 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. Cu2(OH)PO4 Elsevier Electrochemical biosensor Elsevier Near-infrared light Elsevier Nucleic acid Elsevier PhotoATRP Elsevier Xie, Huifang oth Kong, Jinming oth Zhang, Xueji oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:349 year:2021 day:15 month:12 pages:0 https://doi.org/10.1016/j.snb.2021.130797 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 349 2021 15 1215 0 |
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Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). 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Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery |
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cu-mediated nir photoinduced polymerization for highly sensitive electrochemical nucleic acid detection |
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Cu-mediated NIR photoinduced polymerization for highly sensitive electrochemical nucleic acid detection |
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An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. |
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An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. |
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An original electrochemical biosensor via Cu-mediated near-infrared light (NIR) polymerization was reported for highly sensitive nucleic acid detection. Lung cancer DNA as nucleic acid model was specifically recognized via phosphate-free peptide nucleic acid (PNA). Subsequently, initiators of polymerization were attached to PNA/DNA heteroduplex under the assistance of phosphate-Zr4+-carboxylate. Cu2(OH)PO4 and H2O2 system was triggered off under the irradiation of NIR light, and then hydroxyl radicals (•OH) were produced. At the same time, carbon-based radicals were also generated through the reaction between •OH and methacrylic monomers of polymerization, which initiated the photoinduced polymerization. Then, numerous ferrocene-based methacrylic monomers were modified on the surface of electrode. The limit of detection was improved to 2.6 fM with linear range from 0.01 pM to 1000 pM (R2 = 0.993), and the minimum molar number of lung cancer DNA was 26 zmol (in 10 μL). This biosensor also presented portable and cost-efficient feature due to simple operation and ubiquitous light, and no phototoxicity and good tissue penetration of NIR also endowed this strategy great potential in biological detection. |
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Cu-mediated NIR photoinduced polymerization for highly sensitive electrochemical nucleic acid detection |
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