Engineering and development of model lipid membranes mimicking the HeLa cell membrane
Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model memb...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Botet-Carreras, Adrià [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Reaction mechanism investigation of furfural conversion to 2-methylfuran on Cu(1 1 1) surface - Ren, Guoqing ELSEVIER, 2018, an international journal devoted to the principles and applications of colloid and interface science, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:630 ; year:2021 ; day:5 ; month:12 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.colsurfa.2021.127663 |
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| 520 | |a Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. | ||
| 520 | |a Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. | ||
| 650 | 7 | |a Atomic force microscopy (AFM) |2 Elsevier | |
| 650 | 7 | |a HeLa lipid bilayer models |2 Elsevier | |
| 650 | 7 | |a Microviscosity |2 Elsevier | |
| 650 | 7 | |a Förster resonance energy transfer (FRET) |2 Elsevier | |
| 700 | 1 | |a Montero, M. Teresa |4 oth | |
| 700 | 1 | |a Sot, Jesús |4 oth | |
| 700 | 1 | |a Domènech, Òscar |4 oth | |
| 700 | 1 | |a Borrell, Jordi H. |4 oth | |
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10.1016/j.colsurfa.2021.127663 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001783.pica (DE-627)ELV055652905 (ELSEVIER)S0927-7757(21)01532-6 DE-627 ger DE-627 rakwb eng 540 VZ 35.10 bkl Botet-Carreras, Adrià verfasserin aut Engineering and development of model lipid membranes mimicking the HeLa cell membrane 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. Atomic force microscopy (AFM) Elsevier HeLa lipid bilayer models Elsevier Microviscosity Elsevier Förster resonance energy transfer (FRET) Elsevier Montero, M. Teresa oth Sot, Jesús oth Domènech, Òscar oth Borrell, Jordi H. oth Enthalten in Elsevier Science Ren, Guoqing ELSEVIER Reaction mechanism investigation of furfural conversion to 2-methylfuran on Cu(1 1 1) surface 2018 an international journal devoted to the principles and applications of colloid and interface science Amsterdam [u.a.] (DE-627)ELV003763498 volume:630 year:2021 day:5 month:12 pages:0 https://doi.org/10.1016/j.colsurfa.2021.127663 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.10 Physikalische Chemie: Allgemeines VZ AR 630 2021 5 1205 0 |
| spelling |
10.1016/j.colsurfa.2021.127663 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001783.pica (DE-627)ELV055652905 (ELSEVIER)S0927-7757(21)01532-6 DE-627 ger DE-627 rakwb eng 540 VZ 35.10 bkl Botet-Carreras, Adrià verfasserin aut Engineering and development of model lipid membranes mimicking the HeLa cell membrane 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. Atomic force microscopy (AFM) Elsevier HeLa lipid bilayer models Elsevier Microviscosity Elsevier Förster resonance energy transfer (FRET) Elsevier Montero, M. Teresa oth Sot, Jesús oth Domènech, Òscar oth Borrell, Jordi H. oth Enthalten in Elsevier Science Ren, Guoqing ELSEVIER Reaction mechanism investigation of furfural conversion to 2-methylfuran on Cu(1 1 1) surface 2018 an international journal devoted to the principles and applications of colloid and interface science Amsterdam [u.a.] (DE-627)ELV003763498 volume:630 year:2021 day:5 month:12 pages:0 https://doi.org/10.1016/j.colsurfa.2021.127663 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.10 Physikalische Chemie: Allgemeines VZ AR 630 2021 5 1205 0 |
| allfields_unstemmed |
10.1016/j.colsurfa.2021.127663 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001783.pica (DE-627)ELV055652905 (ELSEVIER)S0927-7757(21)01532-6 DE-627 ger DE-627 rakwb eng 540 VZ 35.10 bkl Botet-Carreras, Adrià verfasserin aut Engineering and development of model lipid membranes mimicking the HeLa cell membrane 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. Atomic force microscopy (AFM) Elsevier HeLa lipid bilayer models Elsevier Microviscosity Elsevier Förster resonance energy transfer (FRET) Elsevier Montero, M. Teresa oth Sot, Jesús oth Domènech, Òscar oth Borrell, Jordi H. oth Enthalten in Elsevier Science Ren, Guoqing ELSEVIER Reaction mechanism investigation of furfural conversion to 2-methylfuran on Cu(1 1 1) surface 2018 an international journal devoted to the principles and applications of colloid and interface science Amsterdam [u.a.] (DE-627)ELV003763498 volume:630 year:2021 day:5 month:12 pages:0 https://doi.org/10.1016/j.colsurfa.2021.127663 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.10 Physikalische Chemie: Allgemeines VZ AR 630 2021 5 1205 0 |
| allfieldsGer |
10.1016/j.colsurfa.2021.127663 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001783.pica (DE-627)ELV055652905 (ELSEVIER)S0927-7757(21)01532-6 DE-627 ger DE-627 rakwb eng 540 VZ 35.10 bkl Botet-Carreras, Adrià verfasserin aut Engineering and development of model lipid membranes mimicking the HeLa cell membrane 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. Atomic force microscopy (AFM) Elsevier HeLa lipid bilayer models Elsevier Microviscosity Elsevier Förster resonance energy transfer (FRET) Elsevier Montero, M. Teresa oth Sot, Jesús oth Domènech, Òscar oth Borrell, Jordi H. oth Enthalten in Elsevier Science Ren, Guoqing ELSEVIER Reaction mechanism investigation of furfural conversion to 2-methylfuran on Cu(1 1 1) surface 2018 an international journal devoted to the principles and applications of colloid and interface science Amsterdam [u.a.] (DE-627)ELV003763498 volume:630 year:2021 day:5 month:12 pages:0 https://doi.org/10.1016/j.colsurfa.2021.127663 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.10 Physikalische Chemie: Allgemeines VZ AR 630 2021 5 1205 0 |
| allfieldsSound |
10.1016/j.colsurfa.2021.127663 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001783.pica (DE-627)ELV055652905 (ELSEVIER)S0927-7757(21)01532-6 DE-627 ger DE-627 rakwb eng 540 VZ 35.10 bkl Botet-Carreras, Adrià verfasserin aut Engineering and development of model lipid membranes mimicking the HeLa cell membrane 2021transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. Atomic force microscopy (AFM) Elsevier HeLa lipid bilayer models Elsevier Microviscosity Elsevier Förster resonance energy transfer (FRET) Elsevier Montero, M. Teresa oth Sot, Jesús oth Domènech, Òscar oth Borrell, Jordi H. oth Enthalten in Elsevier Science Ren, Guoqing ELSEVIER Reaction mechanism investigation of furfural conversion to 2-methylfuran on Cu(1 1 1) surface 2018 an international journal devoted to the principles and applications of colloid and interface science Amsterdam [u.a.] (DE-627)ELV003763498 volume:630 year:2021 day:5 month:12 pages:0 https://doi.org/10.1016/j.colsurfa.2021.127663 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.10 Physikalische Chemie: Allgemeines VZ AR 630 2021 5 1205 0 |
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Enthalten in Reaction mechanism investigation of furfural conversion to 2-methylfuran on Cu(1 1 1) surface Amsterdam [u.a.] volume:630 year:2021 day:5 month:12 pages:0 |
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Enthalten in Reaction mechanism investigation of furfural conversion to 2-methylfuran on Cu(1 1 1) surface Amsterdam [u.a.] volume:630 year:2021 day:5 month:12 pages:0 |
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Reaction mechanism investigation of furfural conversion to 2-methylfuran on Cu(1 1 1) surface |
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Botet-Carreras, Adrià @@aut@@ Montero, M. Teresa @@oth@@ Sot, Jesús @@oth@@ Domènech, Òscar @@oth@@ Borrell, Jordi H. @@oth@@ |
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Engineering and development of model lipid membranes mimicking the HeLa cell membrane |
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Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. |
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Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. |
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Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery. |
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Engineering and development of model lipid membranes mimicking the HeLa cell membrane |
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Montero, M. Teresa Sot, Jesús Domènech, Òscar Borrell, Jordi H. |
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