O-methyltransferases catalyze the last step of geniposide biosynthesis in Gardenia jasminoides
Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Xu, Wenjie [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Basal PPARα inhibits bile acid metabolism adaptation in chronic cholestatic model induced by α-naphthylisothiocyanate - Hua, Huiying ELSEVIER, 2018, an international journal, New York, NY [u.a.] |
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| Übergeordnetes Werk: |
volume:177 ; year:2022 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.indcrop.2021.114438 |
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ELV056718918 |
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| 245 | 1 | 0 | |a O-methyltransferases catalyze the last step of geniposide biosynthesis in Gardenia jasminoides |
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| 520 | |a Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. | ||
| 520 | |a Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. | ||
| 650 | 7 | |a Geniposide |2 Elsevier | |
| 650 | 7 | |a Gardenia jasminoides |2 Elsevier | |
| 650 | 7 | |a Transcriptome |2 Elsevier | |
| 650 | 7 | |a Geniposidic acid methyltransferase |2 Elsevier | |
| 650 | 7 | |a Iridoid glycosides |2 Elsevier | |
| 700 | 1 | |a Lou, Qian |4 oth | |
| 700 | 1 | |a Hao, Lijun |4 oth | |
| 700 | 1 | |a Hu, Kaizhi |4 oth | |
| 700 | 1 | |a Cao, Min |4 oth | |
| 700 | 1 | |a Liu, Yanqin |4 oth | |
| 700 | 1 | |a Han, Rongrong |4 oth | |
| 700 | 1 | |a He, Chunnian |4 oth | |
| 700 | 1 | |a Song, Jingyuan |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier |a Hua, Huiying ELSEVIER |t Basal PPARα inhibits bile acid metabolism adaptation in chronic cholestatic model induced by α-naphthylisothiocyanate |d 2018 |d an international journal |g New York, NY [u.a.] |w (DE-627)ELV001103067 |
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10.1016/j.indcrop.2021.114438 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001665.pica (DE-627)ELV056718918 (ELSEVIER)S0926-6690(21)01203-6 DE-627 ger DE-627 rakwb eng 570 540 610 VZ 44.39 bkl Xu, Wenjie verfasserin aut O-methyltransferases catalyze the last step of geniposide biosynthesis in Gardenia jasminoides 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. Geniposide Elsevier Gardenia jasminoides Elsevier Transcriptome Elsevier Geniposidic acid methyltransferase Elsevier Iridoid glycosides Elsevier Lou, Qian oth Hao, Lijun oth Hu, Kaizhi oth Cao, Min oth Liu, Yanqin oth Han, Rongrong oth He, Chunnian oth Song, Jingyuan oth Enthalten in Elsevier Hua, Huiying ELSEVIER Basal PPARα inhibits bile acid metabolism adaptation in chronic cholestatic model induced by α-naphthylisothiocyanate 2018 an international journal New York, NY [u.a.] (DE-627)ELV001103067 volume:177 year:2022 pages:0 https://doi.org/10.1016/j.indcrop.2021.114438 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-PHA 44.39 Toxikologie VZ AR 177 2022 0 |
| spelling |
10.1016/j.indcrop.2021.114438 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001665.pica (DE-627)ELV056718918 (ELSEVIER)S0926-6690(21)01203-6 DE-627 ger DE-627 rakwb eng 570 540 610 VZ 44.39 bkl Xu, Wenjie verfasserin aut O-methyltransferases catalyze the last step of geniposide biosynthesis in Gardenia jasminoides 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. Geniposide Elsevier Gardenia jasminoides Elsevier Transcriptome Elsevier Geniposidic acid methyltransferase Elsevier Iridoid glycosides Elsevier Lou, Qian oth Hao, Lijun oth Hu, Kaizhi oth Cao, Min oth Liu, Yanqin oth Han, Rongrong oth He, Chunnian oth Song, Jingyuan oth Enthalten in Elsevier Hua, Huiying ELSEVIER Basal PPARα inhibits bile acid metabolism adaptation in chronic cholestatic model induced by α-naphthylisothiocyanate 2018 an international journal New York, NY [u.a.] (DE-627)ELV001103067 volume:177 year:2022 pages:0 https://doi.org/10.1016/j.indcrop.2021.114438 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-PHA 44.39 Toxikologie VZ AR 177 2022 0 |
| allfields_unstemmed |
10.1016/j.indcrop.2021.114438 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001665.pica (DE-627)ELV056718918 (ELSEVIER)S0926-6690(21)01203-6 DE-627 ger DE-627 rakwb eng 570 540 610 VZ 44.39 bkl Xu, Wenjie verfasserin aut O-methyltransferases catalyze the last step of geniposide biosynthesis in Gardenia jasminoides 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. Geniposide Elsevier Gardenia jasminoides Elsevier Transcriptome Elsevier Geniposidic acid methyltransferase Elsevier Iridoid glycosides Elsevier Lou, Qian oth Hao, Lijun oth Hu, Kaizhi oth Cao, Min oth Liu, Yanqin oth Han, Rongrong oth He, Chunnian oth Song, Jingyuan oth Enthalten in Elsevier Hua, Huiying ELSEVIER Basal PPARα inhibits bile acid metabolism adaptation in chronic cholestatic model induced by α-naphthylisothiocyanate 2018 an international journal New York, NY [u.a.] (DE-627)ELV001103067 volume:177 year:2022 pages:0 https://doi.org/10.1016/j.indcrop.2021.114438 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-PHA 44.39 Toxikologie VZ AR 177 2022 0 |
| allfieldsGer |
10.1016/j.indcrop.2021.114438 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001665.pica (DE-627)ELV056718918 (ELSEVIER)S0926-6690(21)01203-6 DE-627 ger DE-627 rakwb eng 570 540 610 VZ 44.39 bkl Xu, Wenjie verfasserin aut O-methyltransferases catalyze the last step of geniposide biosynthesis in Gardenia jasminoides 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. Geniposide Elsevier Gardenia jasminoides Elsevier Transcriptome Elsevier Geniposidic acid methyltransferase Elsevier Iridoid glycosides Elsevier Lou, Qian oth Hao, Lijun oth Hu, Kaizhi oth Cao, Min oth Liu, Yanqin oth Han, Rongrong oth He, Chunnian oth Song, Jingyuan oth Enthalten in Elsevier Hua, Huiying ELSEVIER Basal PPARα inhibits bile acid metabolism adaptation in chronic cholestatic model induced by α-naphthylisothiocyanate 2018 an international journal New York, NY [u.a.] (DE-627)ELV001103067 volume:177 year:2022 pages:0 https://doi.org/10.1016/j.indcrop.2021.114438 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-PHA 44.39 Toxikologie VZ AR 177 2022 0 |
| allfieldsSound |
10.1016/j.indcrop.2021.114438 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001665.pica (DE-627)ELV056718918 (ELSEVIER)S0926-6690(21)01203-6 DE-627 ger DE-627 rakwb eng 570 540 610 VZ 44.39 bkl Xu, Wenjie verfasserin aut O-methyltransferases catalyze the last step of geniposide biosynthesis in Gardenia jasminoides 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. Geniposide Elsevier Gardenia jasminoides Elsevier Transcriptome Elsevier Geniposidic acid methyltransferase Elsevier Iridoid glycosides Elsevier Lou, Qian oth Hao, Lijun oth Hu, Kaizhi oth Cao, Min oth Liu, Yanqin oth Han, Rongrong oth He, Chunnian oth Song, Jingyuan oth Enthalten in Elsevier Hua, Huiying ELSEVIER Basal PPARα inhibits bile acid metabolism adaptation in chronic cholestatic model induced by α-naphthylisothiocyanate 2018 an international journal New York, NY [u.a.] (DE-627)ELV001103067 volume:177 year:2022 pages:0 https://doi.org/10.1016/j.indcrop.2021.114438 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-PHA 44.39 Toxikologie VZ AR 177 2022 0 |
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Basal PPARα inhibits bile acid metabolism adaptation in chronic cholestatic model induced by α-naphthylisothiocyanate |
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o-methyltransferases catalyze the last step of geniposide biosynthesis in gardenia jasminoides |
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O-methyltransferases catalyze the last step of geniposide biosynthesis in Gardenia jasminoides |
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Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. |
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Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. |
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Iridoid glycosides, including geniposide, geniposidic acid, and gardenoside, are the main active components of Gardenia jasminoides Ellis. Their biosynthetic pathways have widely attracted researchers’ attention, but yet remain unclear. Elucidating geniposide biosynthesis would help to better define the quality of G. jasminoides plants. In this study, we performed multidimensional transcriptional and metabolic analyses of the leaves and flower buds of G. jasminoides at different developmental stages based on the chromosome-level genome. Ten putative genes involved in geniposide biosynthesis were screened based on phylogenetic analysis and the correlation of gene expression with geniposide accumulation. Two non-tandem geniposidic acid methyltransferase (GAMT) genes were cloned, and in vitro enzymatic reactions with the corresponding recombinant proteins revealed that both could catalyze the conversion of geniposidic acid into geniposide. Synteny analysis showed that these two GAMT genes were unique to G. jasminoides, suggesting the existence of a Gardenia-specific geniposide biosynthetic pathway. In summary, two novel species-specific O-methyltransferases that catalyze the last step of geniposide biosynthesis were identified. These findings will help clarify the biosynthetic pathway of iridoid glycosides and support G. jasminoides breeding. |
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O-methyltransferases catalyze the last step of geniposide biosynthesis in Gardenia jasminoides |
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