Surface enhanced FRET for sensitive and selective detection of doxycycline using organosilicon nanodots as donors
In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Huang, Shuyi [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Neuro-Brucellosis - Gouider, R. ELSEVIER, 2015, an international journal devoted to all branches of analytical chemistry, Amsterdam |
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| Übergeordnetes Werk: |
volume:1197 ; year:2022 ; day:8 ; month:03 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.aca.2022.339530 |
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| 245 | 1 | 0 | |a Surface enhanced FRET for sensitive and selective detection of doxycycline using organosilicon nanodots as donors |
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| 520 | |a In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. | ||
| 520 | |a In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. | ||
| 650 | 7 | |a Organosilicon nanodots |2 Elsevier | |
| 650 | 7 | |a Surface enhanced fluorescence |2 Elsevier | |
| 650 | 7 | |a Doxycycline |2 Elsevier | |
| 650 | 7 | |a Antibiotics detection |2 Elsevier | |
| 700 | 1 | |a Yu, Long |4 oth | |
| 700 | 1 | |a Su, Pengchen |4 oth | |
| 700 | 1 | |a Wen, Tao |4 oth | |
| 700 | 1 | |a Sun, Mingtai |4 oth | |
| 700 | 1 | |a Huang, Dejian |4 oth | |
| 700 | 1 | |a Wang, Xiangke |4 oth | |
| 700 | 1 | |a Wang, Suhua |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Gouider, R. ELSEVIER |t Neuro-Brucellosis |d 2015 |d an international journal devoted to all branches of analytical chemistry |g Amsterdam |w (DE-627)ELV013501887 |
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10.1016/j.aca.2022.339530 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001869.pica (DE-627)ELV056812671 (ELSEVIER)S0003-2670(22)00101-5 DE-627 ger DE-627 rakwb eng 610 VZ 540 VZ 35.10 bkl Huang, Shuyi verfasserin aut Surface enhanced FRET for sensitive and selective detection of doxycycline using organosilicon nanodots as donors 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. Organosilicon nanodots Elsevier Surface enhanced fluorescence Elsevier Doxycycline Elsevier Antibiotics detection Elsevier Yu, Long oth Su, Pengchen oth Wen, Tao oth Sun, Mingtai oth Huang, Dejian oth Wang, Xiangke oth Wang, Suhua oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:1197 year:2022 day:8 month:03 pages:0 https://doi.org/10.1016/j.aca.2022.339530 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 1197 2022 8 0308 0 |
| spelling |
10.1016/j.aca.2022.339530 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001869.pica (DE-627)ELV056812671 (ELSEVIER)S0003-2670(22)00101-5 DE-627 ger DE-627 rakwb eng 610 VZ 540 VZ 35.10 bkl Huang, Shuyi verfasserin aut Surface enhanced FRET for sensitive and selective detection of doxycycline using organosilicon nanodots as donors 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. Organosilicon nanodots Elsevier Surface enhanced fluorescence Elsevier Doxycycline Elsevier Antibiotics detection Elsevier Yu, Long oth Su, Pengchen oth Wen, Tao oth Sun, Mingtai oth Huang, Dejian oth Wang, Xiangke oth Wang, Suhua oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:1197 year:2022 day:8 month:03 pages:0 https://doi.org/10.1016/j.aca.2022.339530 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 1197 2022 8 0308 0 |
| allfields_unstemmed |
10.1016/j.aca.2022.339530 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001869.pica (DE-627)ELV056812671 (ELSEVIER)S0003-2670(22)00101-5 DE-627 ger DE-627 rakwb eng 610 VZ 540 VZ 35.10 bkl Huang, Shuyi verfasserin aut Surface enhanced FRET for sensitive and selective detection of doxycycline using organosilicon nanodots as donors 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. Organosilicon nanodots Elsevier Surface enhanced fluorescence Elsevier Doxycycline Elsevier Antibiotics detection Elsevier Yu, Long oth Su, Pengchen oth Wen, Tao oth Sun, Mingtai oth Huang, Dejian oth Wang, Xiangke oth Wang, Suhua oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:1197 year:2022 day:8 month:03 pages:0 https://doi.org/10.1016/j.aca.2022.339530 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 1197 2022 8 0308 0 |
| allfieldsGer |
10.1016/j.aca.2022.339530 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001869.pica (DE-627)ELV056812671 (ELSEVIER)S0003-2670(22)00101-5 DE-627 ger DE-627 rakwb eng 610 VZ 540 VZ 35.10 bkl Huang, Shuyi verfasserin aut Surface enhanced FRET for sensitive and selective detection of doxycycline using organosilicon nanodots as donors 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. Organosilicon nanodots Elsevier Surface enhanced fluorescence Elsevier Doxycycline Elsevier Antibiotics detection Elsevier Yu, Long oth Su, Pengchen oth Wen, Tao oth Sun, Mingtai oth Huang, Dejian oth Wang, Xiangke oth Wang, Suhua oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:1197 year:2022 day:8 month:03 pages:0 https://doi.org/10.1016/j.aca.2022.339530 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 1197 2022 8 0308 0 |
| allfieldsSound |
10.1016/j.aca.2022.339530 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001869.pica (DE-627)ELV056812671 (ELSEVIER)S0003-2670(22)00101-5 DE-627 ger DE-627 rakwb eng 610 VZ 540 VZ 35.10 bkl Huang, Shuyi verfasserin aut Surface enhanced FRET for sensitive and selective detection of doxycycline using organosilicon nanodots as donors 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. Organosilicon nanodots Elsevier Surface enhanced fluorescence Elsevier Doxycycline Elsevier Antibiotics detection Elsevier Yu, Long oth Su, Pengchen oth Wen, Tao oth Sun, Mingtai oth Huang, Dejian oth Wang, Xiangke oth Wang, Suhua oth Enthalten in Elsevier Science Gouider, R. ELSEVIER Neuro-Brucellosis 2015 an international journal devoted to all branches of analytical chemistry Amsterdam (DE-627)ELV013501887 volume:1197 year:2022 day:8 month:03 pages:0 https://doi.org/10.1016/j.aca.2022.339530 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_40 GBV_ILN_120 35.10 Physikalische Chemie: Allgemeines VZ AR 1197 2022 8 0308 0 |
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This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. 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surface enhanced fret for sensitive and selective detection of doxycycline using organosilicon nanodots as donors |
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Surface enhanced FRET for sensitive and selective detection of doxycycline using organosilicon nanodots as donors |
| abstract |
In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. |
| abstractGer |
In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. |
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In order to achieve on-site fluorescent detection of antibiotic doxycycline (DOC) residue, a new kind of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed by a one-pot strategy and used as a novel ratiometric fluorescent probe. This method could enhance the fluorescence of DOC by conformational fixation on the surface of OSiNDs, and subsequently induced fluorescence resonance energy transfer (FRET). Control experimental results showed that phenylamine functional groups on the as-prepared OSiNDs possessed favorable interactions with DOC by hydrogen bond/π–π stacking, leading to both conformational change of DOC molecules and fluorescence resonance energy transfer process from OSiNDs to DOC. This method was then adapted and optimized for visual sensitive and selective detection of DOC based on ratiometric fluorescence signal, displaying a good linear range for DOC in the range of 1–35 μM with an excellent limit detection of ∼80 nM. Other tetracycline derivatives (TCs) and possible interferences have little influence on the determination of DOC using the method. The spike and recovery test of DOC in real water samples demonstrated the feasibility of the OSiNDs for DOC assay in practical samples and a test paper was fabricated with OSiNDs loading, achieving easy and visual detection of DOC by naked eyes. |
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Surface enhanced FRET for sensitive and selective detection of doxycycline using organosilicon nanodots as donors |
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