Bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of Staphylococcus aureus
Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yin, Wen [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery - Borgès Da Silva, Virginie ELSEVIER, 2018, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:366 ; year:2022 ; day:1 ; month:09 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.snb.2022.132005 |
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| 245 | 1 | 0 | |a Bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of Staphylococcus aureus |
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| 520 | |a Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. | ||
| 520 | |a Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. | ||
| 650 | 7 | |a Horseradish peroxidase (HRP) |2 Elsevier | |
| 650 | 7 | |a Cell wall binding domain (CBD) |2 Elsevier | |
| 650 | 7 | |a Hybrid nanoflower |2 Elsevier | |
| 650 | 7 | |a Detection |2 Elsevier | |
| 650 | 7 | |a Staphylococcus aureus |2 Elsevier | |
| 650 | 7 | |a ELISA |2 Elsevier | |
| 700 | 1 | |a Zhu, Li |4 oth | |
| 700 | 1 | |a Xu, Hui |4 oth | |
| 700 | 1 | |a Tang, Qing |4 oth | |
| 700 | 1 | |a Ma, Yingxin |4 oth | |
| 700 | 1 | |a Chou, Shan-Ho |4 oth | |
| 700 | 1 | |a He, Jin |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Borgès Da Silva, Virginie ELSEVIER |t Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery |d 2018 |g Amsterdam [u.a.] |w (DE-627)ELV001079875 |
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10.1016/j.snb.2022.132005 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001781.pica (DE-627)ELV057800073 (ELSEVIER)S0925-4005(22)00647-5 DE-627 ger DE-627 rakwb eng 610 VZ 44.91 bkl Yin, Wen verfasserin aut Bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of Staphylococcus aureus 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. Horseradish peroxidase (HRP) Elsevier Cell wall binding domain (CBD) Elsevier Hybrid nanoflower Elsevier Detection Elsevier Staphylococcus aureus Elsevier ELISA Elsevier Zhu, Li oth Xu, Hui oth Tang, Qing oth Ma, Yingxin oth Chou, Shan-Ho oth He, Jin oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:366 year:2022 day:1 month:09 pages:0 https://doi.org/10.1016/j.snb.2022.132005 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 366 2022 1 0901 0 |
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10.1016/j.snb.2022.132005 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001781.pica (DE-627)ELV057800073 (ELSEVIER)S0925-4005(22)00647-5 DE-627 ger DE-627 rakwb eng 610 VZ 44.91 bkl Yin, Wen verfasserin aut Bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of Staphylococcus aureus 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. Horseradish peroxidase (HRP) Elsevier Cell wall binding domain (CBD) Elsevier Hybrid nanoflower Elsevier Detection Elsevier Staphylococcus aureus Elsevier ELISA Elsevier Zhu, Li oth Xu, Hui oth Tang, Qing oth Ma, Yingxin oth Chou, Shan-Ho oth He, Jin oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:366 year:2022 day:1 month:09 pages:0 https://doi.org/10.1016/j.snb.2022.132005 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 366 2022 1 0901 0 |
| allfields_unstemmed |
10.1016/j.snb.2022.132005 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001781.pica (DE-627)ELV057800073 (ELSEVIER)S0925-4005(22)00647-5 DE-627 ger DE-627 rakwb eng 610 VZ 44.91 bkl Yin, Wen verfasserin aut Bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of Staphylococcus aureus 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. Horseradish peroxidase (HRP) Elsevier Cell wall binding domain (CBD) Elsevier Hybrid nanoflower Elsevier Detection Elsevier Staphylococcus aureus Elsevier ELISA Elsevier Zhu, Li oth Xu, Hui oth Tang, Qing oth Ma, Yingxin oth Chou, Shan-Ho oth He, Jin oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:366 year:2022 day:1 month:09 pages:0 https://doi.org/10.1016/j.snb.2022.132005 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 366 2022 1 0901 0 |
| allfieldsGer |
10.1016/j.snb.2022.132005 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001781.pica (DE-627)ELV057800073 (ELSEVIER)S0925-4005(22)00647-5 DE-627 ger DE-627 rakwb eng 610 VZ 44.91 bkl Yin, Wen verfasserin aut Bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of Staphylococcus aureus 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. Horseradish peroxidase (HRP) Elsevier Cell wall binding domain (CBD) Elsevier Hybrid nanoflower Elsevier Detection Elsevier Staphylococcus aureus Elsevier ELISA Elsevier Zhu, Li oth Xu, Hui oth Tang, Qing oth Ma, Yingxin oth Chou, Shan-Ho oth He, Jin oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:366 year:2022 day:1 month:09 pages:0 https://doi.org/10.1016/j.snb.2022.132005 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 366 2022 1 0901 0 |
| allfieldsSound |
10.1016/j.snb.2022.132005 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001781.pica (DE-627)ELV057800073 (ELSEVIER)S0925-4005(22)00647-5 DE-627 ger DE-627 rakwb eng 610 VZ 44.91 bkl Yin, Wen verfasserin aut Bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of Staphylococcus aureus 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. Horseradish peroxidase (HRP) Elsevier Cell wall binding domain (CBD) Elsevier Hybrid nanoflower Elsevier Detection Elsevier Staphylococcus aureus Elsevier ELISA Elsevier Zhu, Li oth Xu, Hui oth Tang, Qing oth Ma, Yingxin oth Chou, Shan-Ho oth He, Jin oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:366 year:2022 day:1 month:09 pages:0 https://doi.org/10.1016/j.snb.2022.132005 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 366 2022 1 0901 0 |
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bio-hybrid nanoarchitectonics of nanoflower-based elisa method for the detection of staphylococcus aureus |
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Bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of Staphylococcus aureus |
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Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. |
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Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. |
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Staphylococcus aureus is one of the crucial bacterial pathogens that cause food poisoning and human infectious diseases. Therefore, the establishment of a rapid and sensitive detection method for S. aureus is of great significance for the diagnosis, prevention, and control of the diseases caused by this pathogen. To this end, we have established a bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of S. aureus with high specificity and sensitivity. The core of the method is to prepare a specific cell wall binding domain (CBD)-horseradish peroxidase (HRP)-Cu3(PO4)2 three-in-one organic-inorganic hybrid nanoflower complex through nanoarchitectonics. Detection consists of two main steps: we firstly coated an anti-S. aureus polyclonal antibody into the wells of a 96-well plate to specifically capture S. aureus, and then added customized CBD-HRP-Cu3(PO4)2 organic-inorganic hybrid nanoflower to the system. Due to the high specificity of the CBD from lysin PlyV12 of bacteriophage ϕ1 with S. aureus, a stable antibody-bacterium-hybrid nanoflower complex was formed. Then, the signal amplification of HRP enzyme activity in the hybrid nanoflower was carried out by using the TMB (3,3′,5,5′-tetramethylbenzidine)-hydrogen peroxide reporting system. The method has been proved to be capable of specifically detecting S. aureus ranging from 101 to 106 conoly-forming unit (CFU)/mL linearly with a detection limit down to 6 CFU/mL, which is arguably one of the best for all detection methods reported to date. |
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