Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production
High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 29...
Ausführliche Beschreibung
Autor*in: |
Wang, Xuemin [verfasserIn] |
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E-Artikel |
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Englisch |
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2022transfer abstract |
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11 |
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Übergeordnetes Werk: |
Enthalten in: Technologies and practice of CO - HU, Yongle ELSEVIER, 2019, an international journal : the official journal of WREN, The World Renewable Energy Network, Amsterdam [u.a.] |
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Übergeordnetes Werk: |
volume:197 ; year:2022 ; pages:1133-1143 ; extent:11 |
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DOI / URN: |
10.1016/j.renene.2022.08.002 |
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ELV058838600 |
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245 | 1 | 0 | |a Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production |
264 | 1 | |c 2022transfer abstract | |
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520 | |a High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. | ||
520 | |a High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. | ||
650 | 7 | |a Microbial lipid |2 Elsevier | |
650 | 7 | |a Corn stover |2 Elsevier | |
650 | 7 | |a Fed-batch saccharification |2 Elsevier | |
650 | 7 | |a Fed-batch fermentation |2 Elsevier | |
650 | 7 | |a Cutaneotrichosporon oleaginosum |2 Elsevier | |
650 | 7 | |a Biodiesel |2 Elsevier | |
700 | 1 | |a Wang, Yanan |4 oth | |
700 | 1 | |a He, Qiaoning |4 oth | |
700 | 1 | |a Liu, Yantao |4 oth | |
700 | 1 | |a Zhao, Man |4 oth | |
700 | 1 | |a Liu, Yi |4 oth | |
700 | 1 | |a Zhou, Wenting |4 oth | |
700 | 1 | |a Gong, Zhiwei |4 oth | |
773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a HU, Yongle ELSEVIER |t Technologies and practice of CO |d 2019 |d an international journal : the official journal of WREN, The World Renewable Energy Network |g Amsterdam [u.a.] |w (DE-627)ELV002723662 |
773 | 1 | 8 | |g volume:197 |g year:2022 |g pages:1133-1143 |g extent:11 |
856 | 4 | 0 | |u https://doi.org/10.1016/j.renene.2022.08.002 |3 Volltext |
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10.1016/j.renene.2022.08.002 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001929.pica (DE-627)ELV058838600 (ELSEVIER)S0960-1481(22)01168-5 DE-627 ger DE-627 rakwb eng Wang, Xuemin verfasserin aut Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production 2022transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. Microbial lipid Elsevier Corn stover Elsevier Fed-batch saccharification Elsevier Fed-batch fermentation Elsevier Cutaneotrichosporon oleaginosum Elsevier Biodiesel Elsevier Wang, Yanan oth He, Qiaoning oth Liu, Yantao oth Zhao, Man oth Liu, Yi oth Zhou, Wenting oth Gong, Zhiwei oth Enthalten in Elsevier Science HU, Yongle ELSEVIER Technologies and practice of CO 2019 an international journal : the official journal of WREN, The World Renewable Energy Network Amsterdam [u.a.] (DE-627)ELV002723662 volume:197 year:2022 pages:1133-1143 extent:11 https://doi.org/10.1016/j.renene.2022.08.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U AR 197 2022 1133-1143 11 |
spelling |
10.1016/j.renene.2022.08.002 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001929.pica (DE-627)ELV058838600 (ELSEVIER)S0960-1481(22)01168-5 DE-627 ger DE-627 rakwb eng Wang, Xuemin verfasserin aut Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production 2022transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. Microbial lipid Elsevier Corn stover Elsevier Fed-batch saccharification Elsevier Fed-batch fermentation Elsevier Cutaneotrichosporon oleaginosum Elsevier Biodiesel Elsevier Wang, Yanan oth He, Qiaoning oth Liu, Yantao oth Zhao, Man oth Liu, Yi oth Zhou, Wenting oth Gong, Zhiwei oth Enthalten in Elsevier Science HU, Yongle ELSEVIER Technologies and practice of CO 2019 an international journal : the official journal of WREN, The World Renewable Energy Network Amsterdam [u.a.] (DE-627)ELV002723662 volume:197 year:2022 pages:1133-1143 extent:11 https://doi.org/10.1016/j.renene.2022.08.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U AR 197 2022 1133-1143 11 |
allfields_unstemmed |
10.1016/j.renene.2022.08.002 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001929.pica (DE-627)ELV058838600 (ELSEVIER)S0960-1481(22)01168-5 DE-627 ger DE-627 rakwb eng Wang, Xuemin verfasserin aut Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production 2022transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. Microbial lipid Elsevier Corn stover Elsevier Fed-batch saccharification Elsevier Fed-batch fermentation Elsevier Cutaneotrichosporon oleaginosum Elsevier Biodiesel Elsevier Wang, Yanan oth He, Qiaoning oth Liu, Yantao oth Zhao, Man oth Liu, Yi oth Zhou, Wenting oth Gong, Zhiwei oth Enthalten in Elsevier Science HU, Yongle ELSEVIER Technologies and practice of CO 2019 an international journal : the official journal of WREN, The World Renewable Energy Network Amsterdam [u.a.] (DE-627)ELV002723662 volume:197 year:2022 pages:1133-1143 extent:11 https://doi.org/10.1016/j.renene.2022.08.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U AR 197 2022 1133-1143 11 |
allfieldsGer |
10.1016/j.renene.2022.08.002 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001929.pica (DE-627)ELV058838600 (ELSEVIER)S0960-1481(22)01168-5 DE-627 ger DE-627 rakwb eng Wang, Xuemin verfasserin aut Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production 2022transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. Microbial lipid Elsevier Corn stover Elsevier Fed-batch saccharification Elsevier Fed-batch fermentation Elsevier Cutaneotrichosporon oleaginosum Elsevier Biodiesel Elsevier Wang, Yanan oth He, Qiaoning oth Liu, Yantao oth Zhao, Man oth Liu, Yi oth Zhou, Wenting oth Gong, Zhiwei oth Enthalten in Elsevier Science HU, Yongle ELSEVIER Technologies and practice of CO 2019 an international journal : the official journal of WREN, The World Renewable Energy Network Amsterdam [u.a.] (DE-627)ELV002723662 volume:197 year:2022 pages:1133-1143 extent:11 https://doi.org/10.1016/j.renene.2022.08.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U AR 197 2022 1133-1143 11 |
allfieldsSound |
10.1016/j.renene.2022.08.002 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001929.pica (DE-627)ELV058838600 (ELSEVIER)S0960-1481(22)01168-5 DE-627 ger DE-627 rakwb eng Wang, Xuemin verfasserin aut Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production 2022transfer abstract 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. Microbial lipid Elsevier Corn stover Elsevier Fed-batch saccharification Elsevier Fed-batch fermentation Elsevier Cutaneotrichosporon oleaginosum Elsevier Biodiesel Elsevier Wang, Yanan oth He, Qiaoning oth Liu, Yantao oth Zhao, Man oth Liu, Yi oth Zhou, Wenting oth Gong, Zhiwei oth Enthalten in Elsevier Science HU, Yongle ELSEVIER Technologies and practice of CO 2019 an international journal : the official journal of WREN, The World Renewable Energy Network Amsterdam [u.a.] (DE-627)ELV002723662 volume:197 year:2022 pages:1133-1143 extent:11 https://doi.org/10.1016/j.renene.2022.08.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U AR 197 2022 1133-1143 11 |
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Wang, Xuemin Elsevier Microbial lipid Elsevier Corn stover Elsevier Fed-batch saccharification Elsevier Fed-batch fermentation Elsevier Cutaneotrichosporon oleaginosum Elsevier Biodiesel Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production |
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Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production Microbial lipid Elsevier Corn stover Elsevier Fed-batch saccharification Elsevier Fed-batch fermentation Elsevier Cutaneotrichosporon oleaginosum Elsevier Biodiesel Elsevier |
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highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production |
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Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production |
abstract |
High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. |
abstractGer |
High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. |
abstract_unstemmed |
High-density culture for microbial lipid preparation from low-cost lignocellulosic feedstocks is crucial for commercial-scale biodiesel production. Herein, fed-batch saccharification of alkaline organosolv pretreatment (AOP) of corn stover at an extremely high solids content of 47% (w/v) released 299.5 g/L of lignocellulosic sugars including 18.3% of soluble oligosaccharides. Three types of liquid hydrolysates for seed culture, fermentation, and feeding during fed-batch culture were obtained from the hydrolysate slurry using a two-step washing strategy with 99.3% of sugars recovery. Cutaneotrichosporon oleaginosum showed excellent capacity for assimilating both monosaccharides and oligosaccharides for lipid production using the fed-batch culture mode. Lipid concentration, content, and yield gained 42.3 g/L, 64.6%, and 20.4 g/100 g, respectively. Turbid hydrolysate collected with high recovery of high-concentration sugars and simplified process could be directly served as feeding medium. In general, the overall hydrolysis yield and lipid yield using fed-batch mode accounted for 93.2% and 97.6% of those using batch mode, respectively, resulting in a lipid output of 102.8 g/kg raw corn stover. The fatty acid composition and the prediction of biodiesel properties of lipid samples indicated the suitability for high-quality fuel production. This study provided valuable information for designing highly efficient lignocelluloses-to-biodiesel routes. |
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Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production |
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