Putative biosynthesis mechanism of the neurotoxin β-N-methylamino-L-alanine in marine diatoms based on a transcriptomics approach
The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobut...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Li, Aifeng [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Summer bloom of - Moreira-González, Angel R. ELSEVIER, 2020, environmental control, risk assessment, impact and management, New York, NY [u.a.] |
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| Übergeordnetes Werk: |
volume:441 ; year:2023 ; day:5 ; month:01 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.jhazmat.2022.129953 |
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| 245 | 1 | 0 | |a Putative biosynthesis mechanism of the neurotoxin β-N-methylamino-L-alanine in marine diatoms based on a transcriptomics approach |
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| 520 | |a The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. | ||
| 520 | |a The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. | ||
| 650 | 7 | |a Ubiquitination |2 Elsevier | |
| 650 | 7 | |a β-N-methylamino-L-alanine (BMAA) |2 Elsevier | |
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| 650 | 7 | |a Biosynthesis |2 Elsevier | |
| 650 | 7 | |a Autophagy |2 Elsevier | |
| 700 | 1 | |a Yan, Yeju |4 oth | |
| 700 | 1 | |a Qiu, Jiangbing |4 oth | |
| 700 | 1 | |a Yan, Guowang |4 oth | |
| 700 | 1 | |a Zhao, Peng |4 oth | |
| 700 | 1 | |a Li, Min |4 oth | |
| 700 | 1 | |a Ji, Ying |4 oth | |
| 700 | 1 | |a Wang, Guixiang |4 oth | |
| 700 | 1 | |a Meng, Fanping |4 oth | |
| 700 | 1 | |a Li, Yang |4 oth | |
| 700 | 1 | |a Metcalf, James S. |4 oth | |
| 700 | 1 | |a Banack, Sandra A. |4 oth | |
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10.1016/j.jhazmat.2022.129953 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001940.pica (DE-627)ELV059067918 (ELSEVIER)S0304-3894(22)01747-2 DE-627 ger DE-627 rakwb eng 333.7 610 VZ 43.12 bkl 43.13 bkl 44.13 bkl Li, Aifeng verfasserin aut Putative biosynthesis mechanism of the neurotoxin β-N-methylamino-L-alanine in marine diatoms based on a transcriptomics approach 2023transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. Ubiquitination Elsevier β-N-methylamino-L-alanine (BMAA) Elsevier Diatom Elsevier Biosynthesis Elsevier Autophagy Elsevier Yan, Yeju oth Qiu, Jiangbing oth Yan, Guowang oth Zhao, Peng oth Li, Min oth Ji, Ying oth Wang, Guixiang oth Meng, Fanping oth Li, Yang oth Metcalf, James S. oth Banack, Sandra A. oth Enthalten in Science Direct Moreira-González, Angel R. ELSEVIER Summer bloom of 2020 environmental control, risk assessment, impact and management New York, NY [u.a.] (DE-627)ELV005292484 volume:441 year:2023 day:5 month:01 pages:0 https://doi.org/10.1016/j.jhazmat.2022.129953 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-GGO 43.12 Umweltchemie VZ 43.13 Umwelttoxikologie VZ 44.13 Medizinische Ökologie VZ AR 441 2023 5 0105 0 |
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10.1016/j.jhazmat.2022.129953 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001940.pica (DE-627)ELV059067918 (ELSEVIER)S0304-3894(22)01747-2 DE-627 ger DE-627 rakwb eng 333.7 610 VZ 43.12 bkl 43.13 bkl 44.13 bkl Li, Aifeng verfasserin aut Putative biosynthesis mechanism of the neurotoxin β-N-methylamino-L-alanine in marine diatoms based on a transcriptomics approach 2023transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. Ubiquitination Elsevier β-N-methylamino-L-alanine (BMAA) Elsevier Diatom Elsevier Biosynthesis Elsevier Autophagy Elsevier Yan, Yeju oth Qiu, Jiangbing oth Yan, Guowang oth Zhao, Peng oth Li, Min oth Ji, Ying oth Wang, Guixiang oth Meng, Fanping oth Li, Yang oth Metcalf, James S. oth Banack, Sandra A. oth Enthalten in Science Direct Moreira-González, Angel R. ELSEVIER Summer bloom of 2020 environmental control, risk assessment, impact and management New York, NY [u.a.] (DE-627)ELV005292484 volume:441 year:2023 day:5 month:01 pages:0 https://doi.org/10.1016/j.jhazmat.2022.129953 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-GGO 43.12 Umweltchemie VZ 43.13 Umwelttoxikologie VZ 44.13 Medizinische Ökologie VZ AR 441 2023 5 0105 0 |
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10.1016/j.jhazmat.2022.129953 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001940.pica (DE-627)ELV059067918 (ELSEVIER)S0304-3894(22)01747-2 DE-627 ger DE-627 rakwb eng 333.7 610 VZ 43.12 bkl 43.13 bkl 44.13 bkl Li, Aifeng verfasserin aut Putative biosynthesis mechanism of the neurotoxin β-N-methylamino-L-alanine in marine diatoms based on a transcriptomics approach 2023transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. Ubiquitination Elsevier β-N-methylamino-L-alanine (BMAA) Elsevier Diatom Elsevier Biosynthesis Elsevier Autophagy Elsevier Yan, Yeju oth Qiu, Jiangbing oth Yan, Guowang oth Zhao, Peng oth Li, Min oth Ji, Ying oth Wang, Guixiang oth Meng, Fanping oth Li, Yang oth Metcalf, James S. oth Banack, Sandra A. oth Enthalten in Science Direct Moreira-González, Angel R. ELSEVIER Summer bloom of 2020 environmental control, risk assessment, impact and management New York, NY [u.a.] (DE-627)ELV005292484 volume:441 year:2023 day:5 month:01 pages:0 https://doi.org/10.1016/j.jhazmat.2022.129953 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-GGO 43.12 Umweltchemie VZ 43.13 Umwelttoxikologie VZ 44.13 Medizinische Ökologie VZ AR 441 2023 5 0105 0 |
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10.1016/j.jhazmat.2022.129953 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001940.pica (DE-627)ELV059067918 (ELSEVIER)S0304-3894(22)01747-2 DE-627 ger DE-627 rakwb eng 333.7 610 VZ 43.12 bkl 43.13 bkl 44.13 bkl Li, Aifeng verfasserin aut Putative biosynthesis mechanism of the neurotoxin β-N-methylamino-L-alanine in marine diatoms based on a transcriptomics approach 2023transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. Ubiquitination Elsevier β-N-methylamino-L-alanine (BMAA) Elsevier Diatom Elsevier Biosynthesis Elsevier Autophagy Elsevier Yan, Yeju oth Qiu, Jiangbing oth Yan, Guowang oth Zhao, Peng oth Li, Min oth Ji, Ying oth Wang, Guixiang oth Meng, Fanping oth Li, Yang oth Metcalf, James S. oth Banack, Sandra A. oth Enthalten in Science Direct Moreira-González, Angel R. ELSEVIER Summer bloom of 2020 environmental control, risk assessment, impact and management New York, NY [u.a.] (DE-627)ELV005292484 volume:441 year:2023 day:5 month:01 pages:0 https://doi.org/10.1016/j.jhazmat.2022.129953 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-GGO 43.12 Umweltchemie VZ 43.13 Umwelttoxikologie VZ 44.13 Medizinische Ökologie VZ AR 441 2023 5 0105 0 |
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10.1016/j.jhazmat.2022.129953 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001940.pica (DE-627)ELV059067918 (ELSEVIER)S0304-3894(22)01747-2 DE-627 ger DE-627 rakwb eng 333.7 610 VZ 43.12 bkl 43.13 bkl 44.13 bkl Li, Aifeng verfasserin aut Putative biosynthesis mechanism of the neurotoxin β-N-methylamino-L-alanine in marine diatoms based on a transcriptomics approach 2023transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. Ubiquitination Elsevier β-N-methylamino-L-alanine (BMAA) Elsevier Diatom Elsevier Biosynthesis Elsevier Autophagy Elsevier Yan, Yeju oth Qiu, Jiangbing oth Yan, Guowang oth Zhao, Peng oth Li, Min oth Ji, Ying oth Wang, Guixiang oth Meng, Fanping oth Li, Yang oth Metcalf, James S. oth Banack, Sandra A. oth Enthalten in Science Direct Moreira-González, Angel R. ELSEVIER Summer bloom of 2020 environmental control, risk assessment, impact and management New York, NY [u.a.] (DE-627)ELV005292484 volume:441 year:2023 day:5 month:01 pages:0 https://doi.org/10.1016/j.jhazmat.2022.129953 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA SSG-OPC-GGO 43.12 Umweltchemie VZ 43.13 Umwelttoxikologie VZ 44.13 Medizinische Ökologie VZ AR 441 2023 5 0105 0 |
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Putative biosynthesis mechanism of the neurotoxin β-N-methylamino-L-alanine in marine diatoms based on a transcriptomics approach |
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The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. |
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The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. |
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The neurotoxin β-N-methylamino- L -alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer’s disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms. |
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In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. 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