Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem
The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based m...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lane, Jenna M. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022transfer abstract |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Fabrication of novel hybrid materials based on iron-aluminum modified hemp fibers: Comparison between two proposed methodologies - Viscusi, Gianluca ELSEVIER, 2022, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:175 ; year:2022 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.soilbio.2022.108858 |
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| 245 | 1 | 0 | |a Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem |
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| 520 | |a The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. | ||
| 520 | |a The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. | ||
| 650 | 7 | |a Soil storage methods |2 Elsevier | |
| 650 | 7 | |a Enzyme activity |2 Elsevier | |
| 650 | 7 | |a 16S rRNA sequencing |2 Elsevier | |
| 650 | 7 | |a Bacterial community |2 Elsevier | |
| 700 | 1 | |a Delavaux, Camille S. |4 oth | |
| 700 | 1 | |a Van Koppen, Linsey |4 oth | |
| 700 | 1 | |a Lu, Peina |4 oth | |
| 700 | 1 | |a Cade-Menun, Barbara J. |4 oth | |
| 700 | 1 | |a Tremblay, Julien |4 oth | |
| 700 | 1 | |a Bainard, Luke D. |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Viscusi, Gianluca ELSEVIER |t Fabrication of novel hybrid materials based on iron-aluminum modified hemp fibers: Comparison between two proposed methodologies |d 2022 |g Amsterdam [u.a.] |w (DE-627)ELV007627629 |
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10.1016/j.soilbio.2022.108858 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001953.pica (DE-627)ELV059373512 (ELSEVIER)S0038-0717(22)00315-7 DE-627 ger DE-627 rakwb eng 540 VZ 35.18 bkl 33.68 bkl 52.78 bkl 58.20 bkl Lane, Jenna M. verfasserin aut Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. Soil storage methods Elsevier Enzyme activity Elsevier 16S rRNA sequencing Elsevier Bacterial community Elsevier Delavaux, Camille S. oth Van Koppen, Linsey oth Lu, Peina oth Cade-Menun, Barbara J. oth Tremblay, Julien oth Bainard, Luke D. oth Enthalten in Elsevier Science Viscusi, Gianluca ELSEVIER Fabrication of novel hybrid materials based on iron-aluminum modified hemp fibers: Comparison between two proposed methodologies 2022 Amsterdam [u.a.] (DE-627)ELV007627629 volume:175 year:2022 pages:0 https://doi.org/10.1016/j.soilbio.2022.108858 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.18 Kolloidchemie Grenzflächenchemie VZ 33.68 Oberflächen Dünne Schichten Grenzflächen Physik VZ 52.78 Oberflächentechnik Wärmebehandlung VZ 58.20 Chemische Technologien: Allgemeines VZ AR 175 2022 0 |
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10.1016/j.soilbio.2022.108858 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001953.pica (DE-627)ELV059373512 (ELSEVIER)S0038-0717(22)00315-7 DE-627 ger DE-627 rakwb eng 540 VZ 35.18 bkl 33.68 bkl 52.78 bkl 58.20 bkl Lane, Jenna M. verfasserin aut Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. Soil storage methods Elsevier Enzyme activity Elsevier 16S rRNA sequencing Elsevier Bacterial community Elsevier Delavaux, Camille S. oth Van Koppen, Linsey oth Lu, Peina oth Cade-Menun, Barbara J. oth Tremblay, Julien oth Bainard, Luke D. oth Enthalten in Elsevier Science Viscusi, Gianluca ELSEVIER Fabrication of novel hybrid materials based on iron-aluminum modified hemp fibers: Comparison between two proposed methodologies 2022 Amsterdam [u.a.] (DE-627)ELV007627629 volume:175 year:2022 pages:0 https://doi.org/10.1016/j.soilbio.2022.108858 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.18 Kolloidchemie Grenzflächenchemie VZ 33.68 Oberflächen Dünne Schichten Grenzflächen Physik VZ 52.78 Oberflächentechnik Wärmebehandlung VZ 58.20 Chemische Technologien: Allgemeines VZ AR 175 2022 0 |
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10.1016/j.soilbio.2022.108858 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001953.pica (DE-627)ELV059373512 (ELSEVIER)S0038-0717(22)00315-7 DE-627 ger DE-627 rakwb eng 540 VZ 35.18 bkl 33.68 bkl 52.78 bkl 58.20 bkl Lane, Jenna M. verfasserin aut Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. Soil storage methods Elsevier Enzyme activity Elsevier 16S rRNA sequencing Elsevier Bacterial community Elsevier Delavaux, Camille S. oth Van Koppen, Linsey oth Lu, Peina oth Cade-Menun, Barbara J. oth Tremblay, Julien oth Bainard, Luke D. oth Enthalten in Elsevier Science Viscusi, Gianluca ELSEVIER Fabrication of novel hybrid materials based on iron-aluminum modified hemp fibers: Comparison between two proposed methodologies 2022 Amsterdam [u.a.] (DE-627)ELV007627629 volume:175 year:2022 pages:0 https://doi.org/10.1016/j.soilbio.2022.108858 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.18 Kolloidchemie Grenzflächenchemie VZ 33.68 Oberflächen Dünne Schichten Grenzflächen Physik VZ 52.78 Oberflächentechnik Wärmebehandlung VZ 58.20 Chemische Technologien: Allgemeines VZ AR 175 2022 0 |
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10.1016/j.soilbio.2022.108858 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001953.pica (DE-627)ELV059373512 (ELSEVIER)S0038-0717(22)00315-7 DE-627 ger DE-627 rakwb eng 540 VZ 35.18 bkl 33.68 bkl 52.78 bkl 58.20 bkl Lane, Jenna M. verfasserin aut Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. Soil storage methods Elsevier Enzyme activity Elsevier 16S rRNA sequencing Elsevier Bacterial community Elsevier Delavaux, Camille S. oth Van Koppen, Linsey oth Lu, Peina oth Cade-Menun, Barbara J. oth Tremblay, Julien oth Bainard, Luke D. oth Enthalten in Elsevier Science Viscusi, Gianluca ELSEVIER Fabrication of novel hybrid materials based on iron-aluminum modified hemp fibers: Comparison between two proposed methodologies 2022 Amsterdam [u.a.] (DE-627)ELV007627629 volume:175 year:2022 pages:0 https://doi.org/10.1016/j.soilbio.2022.108858 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.18 Kolloidchemie Grenzflächenchemie VZ 33.68 Oberflächen Dünne Schichten Grenzflächen Physik VZ 52.78 Oberflächentechnik Wärmebehandlung VZ 58.20 Chemische Technologien: Allgemeines VZ AR 175 2022 0 |
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10.1016/j.soilbio.2022.108858 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001953.pica (DE-627)ELV059373512 (ELSEVIER)S0038-0717(22)00315-7 DE-627 ger DE-627 rakwb eng 540 VZ 35.18 bkl 33.68 bkl 52.78 bkl 58.20 bkl Lane, Jenna M. verfasserin aut Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. Soil storage methods Elsevier Enzyme activity Elsevier 16S rRNA sequencing Elsevier Bacterial community Elsevier Delavaux, Camille S. oth Van Koppen, Linsey oth Lu, Peina oth Cade-Menun, Barbara J. oth Tremblay, Julien oth Bainard, Luke D. oth Enthalten in Elsevier Science Viscusi, Gianluca ELSEVIER Fabrication of novel hybrid materials based on iron-aluminum modified hemp fibers: Comparison between two proposed methodologies 2022 Amsterdam [u.a.] (DE-627)ELV007627629 volume:175 year:2022 pages:0 https://doi.org/10.1016/j.soilbio.2022.108858 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.18 Kolloidchemie Grenzflächenchemie VZ 33.68 Oberflächen Dünne Schichten Grenzflächen Physik VZ 52.78 Oberflächentechnik Wärmebehandlung VZ 58.20 Chemische Technologien: Allgemeines VZ AR 175 2022 0 |
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soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem |
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Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem |
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The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. |
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The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. |
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The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies. |
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Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and β-diversity from the controls. Storage at −80 °C was best for maintaining consistent enzyme activity and microbial α- and β-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful information about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage conditions used in future studies.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Soil storage methods</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Enzyme activity</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">16S rRNA sequencing</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Bacterial community</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Delavaux, Camille S.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Van Koppen, Linsey</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Peina</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cade-Menun, Barbara J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tremblay, Julien</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bainard, Luke D.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier Science</subfield><subfield code="a">Viscusi, Gianluca ELSEVIER</subfield><subfield code="t">Fabrication of novel hybrid materials based on iron-aluminum modified hemp fibers: Comparison between two proposed methodologies</subfield><subfield code="d">2022</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV007627629</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:175</subfield><subfield code="g">year:2022</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.soilbio.2022.108858</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">35.18</subfield><subfield code="j">Kolloidchemie</subfield><subfield code="j">Grenzflächenchemie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">33.68</subfield><subfield code="j">Oberflächen</subfield><subfield code="j">Dünne Schichten</subfield><subfield code="j">Grenzflächen</subfield><subfield code="x">Physik</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">52.78</subfield><subfield code="j">Oberflächentechnik</subfield><subfield code="j">Wärmebehandlung</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">58.20</subfield><subfield code="j">Chemische Technologien: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">175</subfield><subfield code="j">2022</subfield><subfield code="h">0</subfield></datafield></record></collection>
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