The importance of defining the age-specific TREC/KREC levels for detection of various inborn errors of immunity in pediatric and adult patients
This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Medova, Veronika [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022transfer abstract |
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| Übergeordnetes Werk: |
Enthalten in: Comorbid episodes of primary bruxism and bruxism as an epileptic activity-related motor event - Bušková, Jitka ELSEVIER, 2019, San Diego, Calif |
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| Übergeordnetes Werk: |
volume:245 ; year:2022 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1016/j.clim.2022.109155 |
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| 520 | |a This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. | ||
| 520 | |a This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. | ||
| 700 | 1 | |a Hulinkova, Ivana |4 oth | |
| 700 | 1 | |a Laiferova, Nikoleta |4 oth | |
| 700 | 1 | |a Urdova, Veronika |4 oth | |
| 700 | 1 | |a Ciznar, Peter |4 oth | |
| 700 | 1 | |a Dolnikova, Dana |4 oth | |
| 700 | 1 | |a Krasnanova, Veronika |4 oth | |
| 700 | 1 | |a Fabri, Oksana |4 oth | |
| 700 | 1 | |a Ficek, Andrej |4 oth | |
| 700 | 1 | |a Soltysova, Andrea |4 oth | |
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10.1016/j.clim.2022.109155 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001971.pica (DE-627)ELV059565500 (ELSEVIER)S1521-6616(22)00236-4 DE-627 ger DE-627 rakwb eng 610 VZ 44.90 bkl Medova, Veronika verfasserin aut The importance of defining the age-specific TREC/KREC levels for detection of various inborn errors of immunity in pediatric and adult patients 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. Hulinkova, Ivana oth Laiferova, Nikoleta oth Urdova, Veronika oth Ciznar, Peter oth Dolnikova, Dana oth Krasnanova, Veronika oth Fabri, Oksana oth Ficek, Andrej oth Soltysova, Andrea oth Enthalten in Elsevier Bušková, Jitka ELSEVIER Comorbid episodes of primary bruxism and bruxism as an epileptic activity-related motor event 2019 San Diego, Calif (DE-627)ELV003196453 volume:245 year:2022 pages:0 https://doi.org/10.1016/j.clim.2022.109155 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.90 Neurologie VZ AR 245 2022 0 |
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10.1016/j.clim.2022.109155 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001971.pica (DE-627)ELV059565500 (ELSEVIER)S1521-6616(22)00236-4 DE-627 ger DE-627 rakwb eng 610 VZ 44.90 bkl Medova, Veronika verfasserin aut The importance of defining the age-specific TREC/KREC levels for detection of various inborn errors of immunity in pediatric and adult patients 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. Hulinkova, Ivana oth Laiferova, Nikoleta oth Urdova, Veronika oth Ciznar, Peter oth Dolnikova, Dana oth Krasnanova, Veronika oth Fabri, Oksana oth Ficek, Andrej oth Soltysova, Andrea oth Enthalten in Elsevier Bušková, Jitka ELSEVIER Comorbid episodes of primary bruxism and bruxism as an epileptic activity-related motor event 2019 San Diego, Calif (DE-627)ELV003196453 volume:245 year:2022 pages:0 https://doi.org/10.1016/j.clim.2022.109155 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.90 Neurologie VZ AR 245 2022 0 |
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10.1016/j.clim.2022.109155 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001971.pica (DE-627)ELV059565500 (ELSEVIER)S1521-6616(22)00236-4 DE-627 ger DE-627 rakwb eng 610 VZ 44.90 bkl Medova, Veronika verfasserin aut The importance of defining the age-specific TREC/KREC levels for detection of various inborn errors of immunity in pediatric and adult patients 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. Hulinkova, Ivana oth Laiferova, Nikoleta oth Urdova, Veronika oth Ciznar, Peter oth Dolnikova, Dana oth Krasnanova, Veronika oth Fabri, Oksana oth Ficek, Andrej oth Soltysova, Andrea oth Enthalten in Elsevier Bušková, Jitka ELSEVIER Comorbid episodes of primary bruxism and bruxism as an epileptic activity-related motor event 2019 San Diego, Calif (DE-627)ELV003196453 volume:245 year:2022 pages:0 https://doi.org/10.1016/j.clim.2022.109155 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.90 Neurologie VZ AR 245 2022 0 |
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10.1016/j.clim.2022.109155 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001971.pica (DE-627)ELV059565500 (ELSEVIER)S1521-6616(22)00236-4 DE-627 ger DE-627 rakwb eng 610 VZ 44.90 bkl Medova, Veronika verfasserin aut The importance of defining the age-specific TREC/KREC levels for detection of various inborn errors of immunity in pediatric and adult patients 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. Hulinkova, Ivana oth Laiferova, Nikoleta oth Urdova, Veronika oth Ciznar, Peter oth Dolnikova, Dana oth Krasnanova, Veronika oth Fabri, Oksana oth Ficek, Andrej oth Soltysova, Andrea oth Enthalten in Elsevier Bušková, Jitka ELSEVIER Comorbid episodes of primary bruxism and bruxism as an epileptic activity-related motor event 2019 San Diego, Calif (DE-627)ELV003196453 volume:245 year:2022 pages:0 https://doi.org/10.1016/j.clim.2022.109155 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.90 Neurologie VZ AR 245 2022 0 |
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10.1016/j.clim.2022.109155 doi /cbs_pica/cbs_olc/import_discovery/elsevier/einzuspielen/GBV00000000001971.pica (DE-627)ELV059565500 (ELSEVIER)S1521-6616(22)00236-4 DE-627 ger DE-627 rakwb eng 610 VZ 44.90 bkl Medova, Veronika verfasserin aut The importance of defining the age-specific TREC/KREC levels for detection of various inborn errors of immunity in pediatric and adult patients 2022transfer abstract nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. Hulinkova, Ivana oth Laiferova, Nikoleta oth Urdova, Veronika oth Ciznar, Peter oth Dolnikova, Dana oth Krasnanova, Veronika oth Fabri, Oksana oth Ficek, Andrej oth Soltysova, Andrea oth Enthalten in Elsevier Bušková, Jitka ELSEVIER Comorbid episodes of primary bruxism and bruxism as an epileptic activity-related motor event 2019 San Diego, Calif (DE-627)ELV003196453 volume:245 year:2022 pages:0 https://doi.org/10.1016/j.clim.2022.109155 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.90 Neurologie VZ AR 245 2022 0 |
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Enthalten in Comorbid episodes of primary bruxism and bruxism as an epileptic activity-related motor event San Diego, Calif volume:245 year:2022 pages:0 |
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Medova, Veronika @@aut@@ Hulinkova, Ivana @@oth@@ Laiferova, Nikoleta @@oth@@ Urdova, Veronika @@oth@@ Ciznar, Peter @@oth@@ Dolnikova, Dana @@oth@@ Krasnanova, Veronika @@oth@@ Fabri, Oksana @@oth@@ Ficek, Andrej @@oth@@ Soltysova, Andrea @@oth@@ |
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The importance of defining the age-specific TREC/KREC levels for detection of various inborn errors of immunity in pediatric and adult patients |
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This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. |
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This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. |
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This study aimed to establish physiological TREC/KREC values in a healthy population of different ages to create cut-offs and analyze pediatric patients with various inborn errors of immunity. Dry blood spots and DNA samples purified from whole blood were used for TREC/KREC quantification using real-time PCR. Observed difference (p < 0.001) between methods revealed the isolation method as a factor we need to consider when determinating cut-offs. Data of 713 healthy individuals showed a negative correlation (p < 0.001) between age and TREC/KREC levels with gender difference observed only for KREC in a group of 51–60 years old (p < 0.001). The 5th percentile cut-off values were set for age groups, which allowed us to identify 25% of patients with immunodeficiencies in case of non-zero, borderline values of TREC/KREC. Screening of infants with congenital heart disease identified 11% of patients with lowered TREC/KREC and shows potential for newborn screening of specific groups of patients. |
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