Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods

A simple PCR-based lateral flow immunoassay (LFIA) was developed in this study to identify rainbow trout ingredient in foods. This assay was developed by coupling PCR amplification of rainbow trout DNA with the detection of labeled amplicon using LFIA. The rainbow trout-specific PCR primers targeted...
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Autor*in:	Ji, Chao [verfasserIn] He, Yihan [verfasserIn] Xing, Yuanying [verfasserIn] Hua, Marti Z. [verfasserIn] Liu, Wei [verfasserIn] Xie, Yuxin [verfasserIn] Ba, Haoran [verfasserIn] Yang, Min [verfasserIn] He, Xiahong [verfasserIn] Zheng, Wenjie [verfasserIn] Lu, Xiaonan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	PCR Lateral flow immunoassay Rainbow trout ingredient Species identification Food adulteration Food authentication

Übergeordnetes Werk:	Enthalten in: Food control - Amsterdam [u.a.] : Elsevier Science, 1990, 154
Übergeordnetes Werk:	volume:154

DOI / URN:	10.1016/j.foodcont.2023.110034

Katalog-ID:	ELV06207427X

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245	1	0	\|a Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods
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520			\|a A simple PCR-based lateral flow immunoassay (LFIA) was developed in this study to identify rainbow trout ingredient in foods. This assay was developed by coupling PCR amplification of rainbow trout DNA with the detection of labeled amplicon using LFIA. The rainbow trout-specific PCR primers targeted at mitochondrial ATP6 gene were successfully designed. The color signals of LFIA could be observed by the naked eye within 5 min. The reliability of this assay was confirmed by quantitative real-time PCR (qPCR). The proposed assay could achieve an LOD at 0.1 ng/μL of rainbow trout DNA. The specificity of the primers was validated against 17 other species related to fish meat fraud and no cross-reactivity was identified, followed by successful test of commercial products for fish meat authentication. PCR-LFIA is simpler and faster than the conventional qPCR and has great potential to be applied to rapidly authenticate rainbow trout ingredient in foods.
650		4	\|a PCR
650		4	\|a Lateral flow immunoassay
650		4	\|a Rainbow trout ingredient
650		4	\|a Species identification
650		4	\|a Food adulteration
650		4	\|a Food authentication
700	1		\|a He, Yihan \|e verfasserin \|0 (orcid)0009-0008-0511-8039 \|4 aut
700	1		\|a Xing, Yuanying \|e verfasserin \|4 aut
700	1		\|a Hua, Marti Z. \|e verfasserin \|4 aut
700	1		\|a Liu, Wei \|e verfasserin \|4 aut
700	1		\|a Xie, Yuxin \|e verfasserin \|4 aut
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700	1		\|a Yang, Min \|e verfasserin \|4 aut
700	1		\|a He, Xiahong \|e verfasserin \|4 aut
700	1		\|a Zheng, Wenjie \|e verfasserin \|4 aut
700	1		\|a Lu, Xiaonan \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Food control \|d Amsterdam [u.a.] : Elsevier Science, 1990 \|g 154 \|h Online-Ressource \|w (DE-627)320604772 \|w (DE-600)2020604-5 \|w (DE-576)259271756 \|x 0956-7135 \|7 nnns
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936	b	k	\|a 58.34 \|j Lebensmitteltechnologie \|q VZ
951			\|a AR
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publishDate	2023
allfields	10.1016/j.foodcont.2023.110034 doi (DE-627)ELV06207427X (ELSEVIER)S0956-7135(23)00434-6 DE-627 ger DE-627 rda eng 630 640 VZ 58.34 bkl Ji, Chao verfasserin aut Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple PCR-based lateral flow immunoassay (LFIA) was developed in this study to identify rainbow trout ingredient in foods. This assay was developed by coupling PCR amplification of rainbow trout DNA with the detection of labeled amplicon using LFIA. The rainbow trout-specific PCR primers targeted at mitochondrial ATP6 gene were successfully designed. The color signals of LFIA could be observed by the naked eye within 5 min. The reliability of this assay was confirmed by quantitative real-time PCR (qPCR). The proposed assay could achieve an LOD at 0.1 ng/μL of rainbow trout DNA. The specificity of the primers was validated against 17 other species related to fish meat fraud and no cross-reactivity was identified, followed by successful test of commercial products for fish meat authentication. PCR-LFIA is simpler and faster than the conventional qPCR and has great potential to be applied to rapidly authenticate rainbow trout ingredient in foods. PCR Lateral flow immunoassay Rainbow trout ingredient Species identification Food adulteration Food authentication He, Yihan verfasserin (orcid)0009-0008-0511-8039 aut Xing, Yuanying verfasserin aut Hua, Marti Z. verfasserin aut Liu, Wei verfasserin aut Xie, Yuxin verfasserin aut Ba, Haoran verfasserin aut Yang, Min verfasserin aut He, Xiahong verfasserin aut Zheng, Wenjie verfasserin aut Lu, Xiaonan verfasserin aut Enthalten in Food control Amsterdam [u.a.] : Elsevier Science, 1990 154 Online-Ressource (DE-627)320604772 (DE-600)2020604-5 (DE-576)259271756 0956-7135 nnns volume:154 GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 154
spelling	10.1016/j.foodcont.2023.110034 doi (DE-627)ELV06207427X (ELSEVIER)S0956-7135(23)00434-6 DE-627 ger DE-627 rda eng 630 640 VZ 58.34 bkl Ji, Chao verfasserin aut Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple PCR-based lateral flow immunoassay (LFIA) was developed in this study to identify rainbow trout ingredient in foods. This assay was developed by coupling PCR amplification of rainbow trout DNA with the detection of labeled amplicon using LFIA. The rainbow trout-specific PCR primers targeted at mitochondrial ATP6 gene were successfully designed. The color signals of LFIA could be observed by the naked eye within 5 min. The reliability of this assay was confirmed by quantitative real-time PCR (qPCR). The proposed assay could achieve an LOD at 0.1 ng/μL of rainbow trout DNA. The specificity of the primers was validated against 17 other species related to fish meat fraud and no cross-reactivity was identified, followed by successful test of commercial products for fish meat authentication. PCR-LFIA is simpler and faster than the conventional qPCR and has great potential to be applied to rapidly authenticate rainbow trout ingredient in foods. PCR Lateral flow immunoassay Rainbow trout ingredient Species identification Food adulteration Food authentication He, Yihan verfasserin (orcid)0009-0008-0511-8039 aut Xing, Yuanying verfasserin aut Hua, Marti Z. verfasserin aut Liu, Wei verfasserin aut Xie, Yuxin verfasserin aut Ba, Haoran verfasserin aut Yang, Min verfasserin aut He, Xiahong verfasserin aut Zheng, Wenjie verfasserin aut Lu, Xiaonan verfasserin aut Enthalten in Food control Amsterdam [u.a.] : Elsevier Science, 1990 154 Online-Ressource (DE-627)320604772 (DE-600)2020604-5 (DE-576)259271756 0956-7135 nnns volume:154 GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 154
allfields_unstemmed	10.1016/j.foodcont.2023.110034 doi (DE-627)ELV06207427X (ELSEVIER)S0956-7135(23)00434-6 DE-627 ger DE-627 rda eng 630 640 VZ 58.34 bkl Ji, Chao verfasserin aut Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple PCR-based lateral flow immunoassay (LFIA) was developed in this study to identify rainbow trout ingredient in foods. This assay was developed by coupling PCR amplification of rainbow trout DNA with the detection of labeled amplicon using LFIA. The rainbow trout-specific PCR primers targeted at mitochondrial ATP6 gene were successfully designed. The color signals of LFIA could be observed by the naked eye within 5 min. The reliability of this assay was confirmed by quantitative real-time PCR (qPCR). The proposed assay could achieve an LOD at 0.1 ng/μL of rainbow trout DNA. The specificity of the primers was validated against 17 other species related to fish meat fraud and no cross-reactivity was identified, followed by successful test of commercial products for fish meat authentication. PCR-LFIA is simpler and faster than the conventional qPCR and has great potential to be applied to rapidly authenticate rainbow trout ingredient in foods. PCR Lateral flow immunoassay Rainbow trout ingredient Species identification Food adulteration Food authentication He, Yihan verfasserin (orcid)0009-0008-0511-8039 aut Xing, Yuanying verfasserin aut Hua, Marti Z. verfasserin aut Liu, Wei verfasserin aut Xie, Yuxin verfasserin aut Ba, Haoran verfasserin aut Yang, Min verfasserin aut He, Xiahong verfasserin aut Zheng, Wenjie verfasserin aut Lu, Xiaonan verfasserin aut Enthalten in Food control Amsterdam [u.a.] : Elsevier Science, 1990 154 Online-Ressource (DE-627)320604772 (DE-600)2020604-5 (DE-576)259271756 0956-7135 nnns volume:154 GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 154
allfieldsGer	10.1016/j.foodcont.2023.110034 doi (DE-627)ELV06207427X (ELSEVIER)S0956-7135(23)00434-6 DE-627 ger DE-627 rda eng 630 640 VZ 58.34 bkl Ji, Chao verfasserin aut Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple PCR-based lateral flow immunoassay (LFIA) was developed in this study to identify rainbow trout ingredient in foods. This assay was developed by coupling PCR amplification of rainbow trout DNA with the detection of labeled amplicon using LFIA. The rainbow trout-specific PCR primers targeted at mitochondrial ATP6 gene were successfully designed. The color signals of LFIA could be observed by the naked eye within 5 min. The reliability of this assay was confirmed by quantitative real-time PCR (qPCR). The proposed assay could achieve an LOD at 0.1 ng/μL of rainbow trout DNA. The specificity of the primers was validated against 17 other species related to fish meat fraud and no cross-reactivity was identified, followed by successful test of commercial products for fish meat authentication. PCR-LFIA is simpler and faster than the conventional qPCR and has great potential to be applied to rapidly authenticate rainbow trout ingredient in foods. PCR Lateral flow immunoassay Rainbow trout ingredient Species identification Food adulteration Food authentication He, Yihan verfasserin (orcid)0009-0008-0511-8039 aut Xing, Yuanying verfasserin aut Hua, Marti Z. verfasserin aut Liu, Wei verfasserin aut Xie, Yuxin verfasserin aut Ba, Haoran verfasserin aut Yang, Min verfasserin aut He, Xiahong verfasserin aut Zheng, Wenjie verfasserin aut Lu, Xiaonan verfasserin aut Enthalten in Food control Amsterdam [u.a.] : Elsevier Science, 1990 154 Online-Ressource (DE-627)320604772 (DE-600)2020604-5 (DE-576)259271756 0956-7135 nnns volume:154 GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 154
allfieldsSound	10.1016/j.foodcont.2023.110034 doi (DE-627)ELV06207427X (ELSEVIER)S0956-7135(23)00434-6 DE-627 ger DE-627 rda eng 630 640 VZ 58.34 bkl Ji, Chao verfasserin aut Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple PCR-based lateral flow immunoassay (LFIA) was developed in this study to identify rainbow trout ingredient in foods. This assay was developed by coupling PCR amplification of rainbow trout DNA with the detection of labeled amplicon using LFIA. The rainbow trout-specific PCR primers targeted at mitochondrial ATP6 gene were successfully designed. The color signals of LFIA could be observed by the naked eye within 5 min. The reliability of this assay was confirmed by quantitative real-time PCR (qPCR). The proposed assay could achieve an LOD at 0.1 ng/μL of rainbow trout DNA. The specificity of the primers was validated against 17 other species related to fish meat fraud and no cross-reactivity was identified, followed by successful test of commercial products for fish meat authentication. PCR-LFIA is simpler and faster than the conventional qPCR and has great potential to be applied to rapidly authenticate rainbow trout ingredient in foods. PCR Lateral flow immunoassay Rainbow trout ingredient Species identification Food adulteration Food authentication He, Yihan verfasserin (orcid)0009-0008-0511-8039 aut Xing, Yuanying verfasserin aut Hua, Marti Z. verfasserin aut Liu, Wei verfasserin aut Xie, Yuxin verfasserin aut Ba, Haoran verfasserin aut Yang, Min verfasserin aut He, Xiahong verfasserin aut Zheng, Wenjie verfasserin aut Lu, Xiaonan verfasserin aut Enthalten in Food control Amsterdam [u.a.] : Elsevier Science, 1990 154 Online-Ressource (DE-627)320604772 (DE-600)2020604-5 (DE-576)259271756 0956-7135 nnns volume:154 GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 154
language	English
source	Enthalten in Food control 154 volume:154
sourceStr	Enthalten in Food control 154 volume:154
format_phy_str_mv	Article
bklname	Lebensmitteltechnologie
institution	findex.gbv.de
topic_facet	PCR Lateral flow immunoassay Rainbow trout ingredient Species identification Food adulteration Food authentication
dewey-raw	630
isfreeaccess_bool	false
container_title	Food control
authorswithroles_txt_mv	Ji, Chao @@aut@@ He, Yihan @@aut@@ Xing, Yuanying @@aut@@ Hua, Marti Z. @@aut@@ Liu, Wei @@aut@@ Xie, Yuxin @@aut@@ Ba, Haoran @@aut@@ Yang, Min @@aut@@ He, Xiahong @@aut@@ Zheng, Wenjie @@aut@@ Lu, Xiaonan @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	320604772
dewey-sort	3630
id	ELV06207427X
language_de	englisch
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author	Ji, Chao
spellingShingle	Ji, Chao ddc 630 bkl 58.34 misc PCR misc Lateral flow immunoassay misc Rainbow trout ingredient misc Species identification misc Food adulteration misc Food authentication Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods
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topic_title	630 640 VZ 58.34 bkl Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods PCR Lateral flow immunoassay Rainbow trout ingredient Species identification Food adulteration Food authentication
topic	ddc 630 bkl 58.34 misc PCR misc Lateral flow immunoassay misc Rainbow trout ingredient misc Species identification misc Food adulteration misc Food authentication
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title	Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods
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title_full	Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods
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author_browse	Ji, Chao He, Yihan Xing, Yuanying Hua, Marti Z. Liu, Wei Xie, Yuxin Ba, Haoran Yang, Min He, Xiahong Zheng, Wenjie Lu, Xiaonan
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title_sort	development of a pcr-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods
title_auth	Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods
abstract	A simple PCR-based lateral flow immunoassay (LFIA) was developed in this study to identify rainbow trout ingredient in foods. This assay was developed by coupling PCR amplification of rainbow trout DNA with the detection of labeled amplicon using LFIA. The rainbow trout-specific PCR primers targeted at mitochondrial ATP6 gene were successfully designed. The color signals of LFIA could be observed by the naked eye within 5 min. The reliability of this assay was confirmed by quantitative real-time PCR (qPCR). The proposed assay could achieve an LOD at 0.1 ng/μL of rainbow trout DNA. The specificity of the primers was validated against 17 other species related to fish meat fraud and no cross-reactivity was identified, followed by successful test of commercial products for fish meat authentication. PCR-LFIA is simpler and faster than the conventional qPCR and has great potential to be applied to rapidly authenticate rainbow trout ingredient in foods.
abstractGer	A simple PCR-based lateral flow immunoassay (LFIA) was developed in this study to identify rainbow trout ingredient in foods. This assay was developed by coupling PCR amplification of rainbow trout DNA with the detection of labeled amplicon using LFIA. The rainbow trout-specific PCR primers targeted at mitochondrial ATP6 gene were successfully designed. The color signals of LFIA could be observed by the naked eye within 5 min. The reliability of this assay was confirmed by quantitative real-time PCR (qPCR). The proposed assay could achieve an LOD at 0.1 ng/μL of rainbow trout DNA. The specificity of the primers was validated against 17 other species related to fish meat fraud and no cross-reactivity was identified, followed by successful test of commercial products for fish meat authentication. PCR-LFIA is simpler and faster than the conventional qPCR and has great potential to be applied to rapidly authenticate rainbow trout ingredient in foods.
abstract_unstemmed	A simple PCR-based lateral flow immunoassay (LFIA) was developed in this study to identify rainbow trout ingredient in foods. This assay was developed by coupling PCR amplification of rainbow trout DNA with the detection of labeled amplicon using LFIA. The rainbow trout-specific PCR primers targeted at mitochondrial ATP6 gene were successfully designed. The color signals of LFIA could be observed by the naked eye within 5 min. The reliability of this assay was confirmed by quantitative real-time PCR (qPCR). The proposed assay could achieve an LOD at 0.1 ng/μL of rainbow trout DNA. The specificity of the primers was validated against 17 other species related to fish meat fraud and no cross-reactivity was identified, followed by successful test of commercial products for fish meat authentication. PCR-LFIA is simpler and faster than the conventional qPCR and has great potential to be applied to rapidly authenticate rainbow trout ingredient in foods.
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title_short	Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods
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author2	He, Yihan Xing, Yuanying Hua, Marti Z. Liu, Wei Xie, Yuxin Ba, Haoran Yang, Min He, Xiahong Zheng, Wenjie Lu, Xiaonan
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doi_str	10.1016/j.foodcont.2023.110034
up_date	2024-07-06T18:22:36.580Z
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Development of a PCR-based lateral flow immunoassay for the identification of rainbow trout ingredient in foods

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