How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides

Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide...
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Gespeichert in:

Autor*in:	Guzmán-Ortiz, Fabiola Araceli [verfasserIn] Peñas, Elena [verfasserIn] Frias, Juana [verfasserIn] Castro-Rosas, Javier [verfasserIn] Martínez-Villaluenga, Cristina [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Germination time Protein digestion Bioactive peptides INFOGEST Inflammation

Übergeordnetes Werk:	Enthalten in: Food chemistry - New York, NY [u.a.] : Elsevier, 1976, 433
Übergeordnetes Werk:	volume:433

DOI / URN:	10.1016/j.foodchem.2023.137343

Katalog-ID:	ELV064965031

Internformat


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520			\|a Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time for maximum nutritional and health benefits. Protein hydrolysis was monitored during germination and digestion by gel electrophoresis, size exclusion chromatography, and the analysis of soluble protein (SP), peptides (PEP), free amino acids (FAA) and free amino groups. The anti-inflammatory activity of intestinal digests was investigated in cell culture assays. Peptidomic and in silico analyses of intestinal digesta were conducted to identify digestion-resistant bioactive fragments. Germination time increased SP, PEP, and FAA. During digestion, the PH and release of small peptides was higher in sprouted lupin than control flour. Intestinal digests from sprouted lupin flour for 7 days exhibited the highest anti-inflammatory activity. In this sample, 11 potential bioactive peptides were identified. These findings open the exploration of novel food formulations based on sprouted lupins with higher protein digestibility and health-promoting potential.
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publishDate	2023
allfields	10.1016/j.foodchem.2023.137343 doi (DE-627)ELV064965031 (ELSEVIER)S0308-8146(23)01961-1 DE-627 ger DE-627 rda eng 540 660 VZ 58.34 bkl Guzmán-Ortiz, Fabiola Araceli verfasserin aut How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time for maximum nutritional and health benefits. Protein hydrolysis was monitored during germination and digestion by gel electrophoresis, size exclusion chromatography, and the analysis of soluble protein (SP), peptides (PEP), free amino acids (FAA) and free amino groups. The anti-inflammatory activity of intestinal digests was investigated in cell culture assays. Peptidomic and in silico analyses of intestinal digesta were conducted to identify digestion-resistant bioactive fragments. Germination time increased SP, PEP, and FAA. During digestion, the PH and release of small peptides was higher in sprouted lupin than control flour. Intestinal digests from sprouted lupin flour for 7 days exhibited the highest anti-inflammatory activity. In this sample, 11 potential bioactive peptides were identified. These findings open the exploration of novel food formulations based on sprouted lupins with higher protein digestibility and health-promoting potential. Germination time Protein digestion Bioactive peptides INFOGEST Inflammation Peñas, Elena verfasserin aut Frias, Juana verfasserin aut Castro-Rosas, Javier verfasserin aut Martínez-Villaluenga, Cristina verfasserin aut Enthalten in Food chemistry New York, NY [u.a.] : Elsevier, 1976 433 Online-Ressource (DE-627)300898509 (DE-600)1483647-6 (DE-576)098330225 1873-7072 nnns volume:433 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 433
spelling	10.1016/j.foodchem.2023.137343 doi (DE-627)ELV064965031 (ELSEVIER)S0308-8146(23)01961-1 DE-627 ger DE-627 rda eng 540 660 VZ 58.34 bkl Guzmán-Ortiz, Fabiola Araceli verfasserin aut How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time for maximum nutritional and health benefits. Protein hydrolysis was monitored during germination and digestion by gel electrophoresis, size exclusion chromatography, and the analysis of soluble protein (SP), peptides (PEP), free amino acids (FAA) and free amino groups. The anti-inflammatory activity of intestinal digests was investigated in cell culture assays. Peptidomic and in silico analyses of intestinal digesta were conducted to identify digestion-resistant bioactive fragments. Germination time increased SP, PEP, and FAA. During digestion, the PH and release of small peptides was higher in sprouted lupin than control flour. Intestinal digests from sprouted lupin flour for 7 days exhibited the highest anti-inflammatory activity. In this sample, 11 potential bioactive peptides were identified. These findings open the exploration of novel food formulations based on sprouted lupins with higher protein digestibility and health-promoting potential. Germination time Protein digestion Bioactive peptides INFOGEST Inflammation Peñas, Elena verfasserin aut Frias, Juana verfasserin aut Castro-Rosas, Javier verfasserin aut Martínez-Villaluenga, Cristina verfasserin aut Enthalten in Food chemistry New York, NY [u.a.] : Elsevier, 1976 433 Online-Ressource (DE-627)300898509 (DE-600)1483647-6 (DE-576)098330225 1873-7072 nnns volume:433 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 433
allfields_unstemmed	10.1016/j.foodchem.2023.137343 doi (DE-627)ELV064965031 (ELSEVIER)S0308-8146(23)01961-1 DE-627 ger DE-627 rda eng 540 660 VZ 58.34 bkl Guzmán-Ortiz, Fabiola Araceli verfasserin aut How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time for maximum nutritional and health benefits. Protein hydrolysis was monitored during germination and digestion by gel electrophoresis, size exclusion chromatography, and the analysis of soluble protein (SP), peptides (PEP), free amino acids (FAA) and free amino groups. The anti-inflammatory activity of intestinal digests was investigated in cell culture assays. Peptidomic and in silico analyses of intestinal digesta were conducted to identify digestion-resistant bioactive fragments. Germination time increased SP, PEP, and FAA. During digestion, the PH and release of small peptides was higher in sprouted lupin than control flour. Intestinal digests from sprouted lupin flour for 7 days exhibited the highest anti-inflammatory activity. In this sample, 11 potential bioactive peptides were identified. These findings open the exploration of novel food formulations based on sprouted lupins with higher protein digestibility and health-promoting potential. Germination time Protein digestion Bioactive peptides INFOGEST Inflammation Peñas, Elena verfasserin aut Frias, Juana verfasserin aut Castro-Rosas, Javier verfasserin aut Martínez-Villaluenga, Cristina verfasserin aut Enthalten in Food chemistry New York, NY [u.a.] : Elsevier, 1976 433 Online-Ressource (DE-627)300898509 (DE-600)1483647-6 (DE-576)098330225 1873-7072 nnns volume:433 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 433
allfieldsGer	10.1016/j.foodchem.2023.137343 doi (DE-627)ELV064965031 (ELSEVIER)S0308-8146(23)01961-1 DE-627 ger DE-627 rda eng 540 660 VZ 58.34 bkl Guzmán-Ortiz, Fabiola Araceli verfasserin aut How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time for maximum nutritional and health benefits. Protein hydrolysis was monitored during germination and digestion by gel electrophoresis, size exclusion chromatography, and the analysis of soluble protein (SP), peptides (PEP), free amino acids (FAA) and free amino groups. The anti-inflammatory activity of intestinal digests was investigated in cell culture assays. Peptidomic and in silico analyses of intestinal digesta were conducted to identify digestion-resistant bioactive fragments. Germination time increased SP, PEP, and FAA. During digestion, the PH and release of small peptides was higher in sprouted lupin than control flour. Intestinal digests from sprouted lupin flour for 7 days exhibited the highest anti-inflammatory activity. In this sample, 11 potential bioactive peptides were identified. These findings open the exploration of novel food formulations based on sprouted lupins with higher protein digestibility and health-promoting potential. Germination time Protein digestion Bioactive peptides INFOGEST Inflammation Peñas, Elena verfasserin aut Frias, Juana verfasserin aut Castro-Rosas, Javier verfasserin aut Martínez-Villaluenga, Cristina verfasserin aut Enthalten in Food chemistry New York, NY [u.a.] : Elsevier, 1976 433 Online-Ressource (DE-627)300898509 (DE-600)1483647-6 (DE-576)098330225 1873-7072 nnns volume:433 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 433
allfieldsSound	10.1016/j.foodchem.2023.137343 doi (DE-627)ELV064965031 (ELSEVIER)S0308-8146(23)01961-1 DE-627 ger DE-627 rda eng 540 660 VZ 58.34 bkl Guzmán-Ortiz, Fabiola Araceli verfasserin aut How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time for maximum nutritional and health benefits. Protein hydrolysis was monitored during germination and digestion by gel electrophoresis, size exclusion chromatography, and the analysis of soluble protein (SP), peptides (PEP), free amino acids (FAA) and free amino groups. The anti-inflammatory activity of intestinal digests was investigated in cell culture assays. Peptidomic and in silico analyses of intestinal digesta were conducted to identify digestion-resistant bioactive fragments. Germination time increased SP, PEP, and FAA. During digestion, the PH and release of small peptides was higher in sprouted lupin than control flour. Intestinal digests from sprouted lupin flour for 7 days exhibited the highest anti-inflammatory activity. In this sample, 11 potential bioactive peptides were identified. These findings open the exploration of novel food formulations based on sprouted lupins with higher protein digestibility and health-promoting potential. Germination time Protein digestion Bioactive peptides INFOGEST Inflammation Peñas, Elena verfasserin aut Frias, Juana verfasserin aut Castro-Rosas, Javier verfasserin aut Martínez-Villaluenga, Cristina verfasserin aut Enthalten in Food chemistry New York, NY [u.a.] : Elsevier, 1976 433 Online-Ressource (DE-627)300898509 (DE-600)1483647-6 (DE-576)098330225 1873-7072 nnns volume:433 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 433
language	English
source	Enthalten in Food chemistry 433 volume:433
sourceStr	Enthalten in Food chemistry 433 volume:433
format_phy_str_mv	Article
bklname	Lebensmitteltechnologie
institution	findex.gbv.de
topic_facet	Germination time Protein digestion Bioactive peptides INFOGEST Inflammation
dewey-raw	540
isfreeaccess_bool	false
container_title	Food chemistry
authorswithroles_txt_mv	Guzmán-Ortiz, Fabiola Araceli @@aut@@ Peñas, Elena @@aut@@ Frias, Juana @@aut@@ Castro-Rosas, Javier @@aut@@ Martínez-Villaluenga, Cristina @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	300898509
dewey-sort	3540
id	ELV064965031
language_de	englisch
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author	Guzmán-Ortiz, Fabiola Araceli
spellingShingle	Guzmán-Ortiz, Fabiola Araceli ddc 540 bkl 58.34 misc Germination time misc Protein digestion misc Bioactive peptides misc INFOGEST misc Inflammation How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides
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topic_title	540 660 VZ 58.34 bkl How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides Germination time Protein digestion Bioactive peptides INFOGEST Inflammation
topic	ddc 540 bkl 58.34 misc Germination time misc Protein digestion misc Bioactive peptides misc INFOGEST misc Inflammation
topic_unstemmed	ddc 540 bkl 58.34 misc Germination time misc Protein digestion misc Bioactive peptides misc INFOGEST misc Inflammation
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title	How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides
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title_full	How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides
author_sort	Guzmán-Ortiz, Fabiola Araceli
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title_sort	how germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides
title_auth	How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides
abstract	Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time for maximum nutritional and health benefits. Protein hydrolysis was monitored during germination and digestion by gel electrophoresis, size exclusion chromatography, and the analysis of soluble protein (SP), peptides (PEP), free amino acids (FAA) and free amino groups. The anti-inflammatory activity of intestinal digests was investigated in cell culture assays. Peptidomic and in silico analyses of intestinal digesta were conducted to identify digestion-resistant bioactive fragments. Germination time increased SP, PEP, and FAA. During digestion, the PH and release of small peptides was higher in sprouted lupin than control flour. Intestinal digests from sprouted lupin flour for 7 days exhibited the highest anti-inflammatory activity. In this sample, 11 potential bioactive peptides were identified. These findings open the exploration of novel food formulations based on sprouted lupins with higher protein digestibility and health-promoting potential.
abstractGer	Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time for maximum nutritional and health benefits. Protein hydrolysis was monitored during germination and digestion by gel electrophoresis, size exclusion chromatography, and the analysis of soluble protein (SP), peptides (PEP), free amino acids (FAA) and free amino groups. The anti-inflammatory activity of intestinal digests was investigated in cell culture assays. Peptidomic and in silico analyses of intestinal digesta were conducted to identify digestion-resistant bioactive fragments. Germination time increased SP, PEP, and FAA. During digestion, the PH and release of small peptides was higher in sprouted lupin than control flour. Intestinal digests from sprouted lupin flour for 7 days exhibited the highest anti-inflammatory activity. In this sample, 11 potential bioactive peptides were identified. These findings open the exploration of novel food formulations based on sprouted lupins with higher protein digestibility and health-promoting potential.
abstract_unstemmed	Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time for maximum nutritional and health benefits. Protein hydrolysis was monitored during germination and digestion by gel electrophoresis, size exclusion chromatography, and the analysis of soluble protein (SP), peptides (PEP), free amino acids (FAA) and free amino groups. The anti-inflammatory activity of intestinal digests was investigated in cell culture assays. Peptidomic and in silico analyses of intestinal digesta were conducted to identify digestion-resistant bioactive fragments. Germination time increased SP, PEP, and FAA. During digestion, the PH and release of small peptides was higher in sprouted lupin than control flour. Intestinal digests from sprouted lupin flour for 7 days exhibited the highest anti-inflammatory activity. In this sample, 11 potential bioactive peptides were identified. These findings open the exploration of novel food formulations based on sprouted lupins with higher protein digestibility and health-promoting potential.
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title_short	How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides
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author2	Peñas, Elena Frias, Juana Castro-Rosas, Javier Martínez-Villaluenga, Cristina
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up_date	2024-07-06T21:21:07.882Z
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How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides

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