Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection
As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission elect...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Xiang, Jiawang [verfasserIn] Zhang, Bing [verfasserIn] Shi, Yani [verfasserIn] Wen, Yanfei [verfasserIn] Yuan, Yuan [verfasserIn] Lin, Jianying [verfasserIn] Zhao, Zhihuan [verfasserIn] Li, Jing [verfasserIn] Cheng, Yan [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Analytical biochemistry - San Diego, Calif. : Elsevier, 1960, 683 |
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| Übergeordnetes Werk: |
volume:683 |
| DOI / URN: |
10.1016/j.ab.2023.115370 |
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| Katalog-ID: |
ELV065505735 |
|---|
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| 245 | 1 | 0 | |a Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection |
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| 520 | |a As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL−1 to 40 ng mL−1, and the detection limit is 0.08 ng mL−1. Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. | ||
| 650 | 4 | |a Photothermal | |
| 650 | 4 | |a Immunoassay | |
| 650 | 4 | |a Carcinoembryonic antigen | |
| 650 | 4 | |a Molybdenum blue | |
| 650 | 4 | |a Isoniazide modified Ag nanoparticles | |
| 700 | 1 | |a Zhang, Bing |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Yani |e verfasserin |4 aut | |
| 700 | 1 | |a Wen, Yanfei |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Jianying |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Zhihuan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Yan |e verfasserin |4 aut | |
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10.1016/j.ab.2023.115370 doi (DE-627)ELV065505735 (ELSEVIER)S0003-2697(23)00335-4 DE-627 ger DE-627 rda eng 570 540 VZ 12 ssgn 35.71 bkl Xiang, Jiawang verfasserin aut Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL−1 to 40 ng mL−1, and the detection limit is 0.08 ng mL−1. Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. Photothermal Immunoassay Carcinoembryonic antigen Molybdenum blue Isoniazide modified Ag nanoparticles Zhang, Bing verfasserin aut Shi, Yani verfasserin aut Wen, Yanfei verfasserin aut Yuan, Yuan verfasserin aut Lin, Jianying verfasserin aut Zhao, Zhihuan verfasserin aut Li, Jing verfasserin aut Cheng, Yan verfasserin aut Enthalten in Analytical biochemistry San Diego, Calif. : Elsevier, 1960 683 Online-Ressource (DE-627)25378641X (DE-600)1461105-3 (DE-576)103372997 1096-0309 nnns volume:683 GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.71 Biochemische Methoden VZ AR 683 |
| spelling |
10.1016/j.ab.2023.115370 doi (DE-627)ELV065505735 (ELSEVIER)S0003-2697(23)00335-4 DE-627 ger DE-627 rda eng 570 540 VZ 12 ssgn 35.71 bkl Xiang, Jiawang verfasserin aut Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL−1 to 40 ng mL−1, and the detection limit is 0.08 ng mL−1. Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. Photothermal Immunoassay Carcinoembryonic antigen Molybdenum blue Isoniazide modified Ag nanoparticles Zhang, Bing verfasserin aut Shi, Yani verfasserin aut Wen, Yanfei verfasserin aut Yuan, Yuan verfasserin aut Lin, Jianying verfasserin aut Zhao, Zhihuan verfasserin aut Li, Jing verfasserin aut Cheng, Yan verfasserin aut Enthalten in Analytical biochemistry San Diego, Calif. : Elsevier, 1960 683 Online-Ressource (DE-627)25378641X (DE-600)1461105-3 (DE-576)103372997 1096-0309 nnns volume:683 GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.71 Biochemische Methoden VZ AR 683 |
| allfields_unstemmed |
10.1016/j.ab.2023.115370 doi (DE-627)ELV065505735 (ELSEVIER)S0003-2697(23)00335-4 DE-627 ger DE-627 rda eng 570 540 VZ 12 ssgn 35.71 bkl Xiang, Jiawang verfasserin aut Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL−1 to 40 ng mL−1, and the detection limit is 0.08 ng mL−1. Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. Photothermal Immunoassay Carcinoembryonic antigen Molybdenum blue Isoniazide modified Ag nanoparticles Zhang, Bing verfasserin aut Shi, Yani verfasserin aut Wen, Yanfei verfasserin aut Yuan, Yuan verfasserin aut Lin, Jianying verfasserin aut Zhao, Zhihuan verfasserin aut Li, Jing verfasserin aut Cheng, Yan verfasserin aut Enthalten in Analytical biochemistry San Diego, Calif. : Elsevier, 1960 683 Online-Ressource (DE-627)25378641X (DE-600)1461105-3 (DE-576)103372997 1096-0309 nnns volume:683 GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.71 Biochemische Methoden VZ AR 683 |
| allfieldsGer |
10.1016/j.ab.2023.115370 doi (DE-627)ELV065505735 (ELSEVIER)S0003-2697(23)00335-4 DE-627 ger DE-627 rda eng 570 540 VZ 12 ssgn 35.71 bkl Xiang, Jiawang verfasserin aut Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL−1 to 40 ng mL−1, and the detection limit is 0.08 ng mL−1. Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. Photothermal Immunoassay Carcinoembryonic antigen Molybdenum blue Isoniazide modified Ag nanoparticles Zhang, Bing verfasserin aut Shi, Yani verfasserin aut Wen, Yanfei verfasserin aut Yuan, Yuan verfasserin aut Lin, Jianying verfasserin aut Zhao, Zhihuan verfasserin aut Li, Jing verfasserin aut Cheng, Yan verfasserin aut Enthalten in Analytical biochemistry San Diego, Calif. : Elsevier, 1960 683 Online-Ressource (DE-627)25378641X (DE-600)1461105-3 (DE-576)103372997 1096-0309 nnns volume:683 GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.71 Biochemische Methoden VZ AR 683 |
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10.1016/j.ab.2023.115370 doi (DE-627)ELV065505735 (ELSEVIER)S0003-2697(23)00335-4 DE-627 ger DE-627 rda eng 570 540 VZ 12 ssgn 35.71 bkl Xiang, Jiawang verfasserin aut Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL−1 to 40 ng mL−1, and the detection limit is 0.08 ng mL−1. Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. Photothermal Immunoassay Carcinoembryonic antigen Molybdenum blue Isoniazide modified Ag nanoparticles Zhang, Bing verfasserin aut Shi, Yani verfasserin aut Wen, Yanfei verfasserin aut Yuan, Yuan verfasserin aut Lin, Jianying verfasserin aut Zhao, Zhihuan verfasserin aut Li, Jing verfasserin aut Cheng, Yan verfasserin aut Enthalten in Analytical biochemistry San Diego, Calif. : Elsevier, 1960 683 Online-Ressource (DE-627)25378641X (DE-600)1461105-3 (DE-576)103372997 1096-0309 nnns volume:683 GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.71 Biochemische Methoden VZ AR 683 |
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| author |
Xiang, Jiawang |
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Xiang, Jiawang ddc 570 ssgn 12 bkl 35.71 misc Photothermal misc Immunoassay misc Carcinoembryonic antigen misc Molybdenum blue misc Isoniazide modified Ag nanoparticles Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection |
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570 540 VZ 12 ssgn 35.71 bkl Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection Photothermal Immunoassay Carcinoembryonic antigen Molybdenum blue Isoniazide modified Ag nanoparticles |
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Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection |
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Xiang, Jiawang Zhang, Bing Shi, Yani Wen, Yanfei Yuan, Yuan Lin, Jianying Zhao, Zhihuan Li, Jing Cheng, Yan |
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isoniazide modified ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection |
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Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection |
| abstract |
As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL−1 to 40 ng mL−1, and the detection limit is 0.08 ng mL−1. Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. |
| abstractGer |
As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL−1 to 40 ng mL−1, and the detection limit is 0.08 ng mL−1. Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. |
| abstract_unstemmed |
As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL−1 to 40 ng mL−1, and the detection limit is 0.08 ng mL−1. Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. |
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Zhang, Bing Shi, Yani Wen, Yanfei Yuan, Yuan Lin, Jianying Zhao, Zhihuan Li, Jing Cheng, Yan |
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