Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia

Schizophrenia is a serious mental disease with unknown etiology that affects approximately 1 % of the population around the world. Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatograp...
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Autor*in:	Wang, Juan [verfasserIn] Cui, Li [verfasserIn] Pan, Guoliang [verfasserIn] Li, Ying [verfasserIn] Sun, Xiaoyu [verfasserIn] Zhang, Yalian [verfasserIn] Zhao, Furong [verfasserIn] Cao, Yunfeng [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Amino acid neurotransmitters Human plasma Liquid chromatography-tandem mass spectrometry Reference intervals Schizophrenia

Übergeordnetes Werk:	Enthalten in: Clinica chimica acta - Amsterdam [u.a.] : Elsevier Science, 1956, 552
Übergeordnetes Werk:	volume:552

DOI / URN:	10.1016/j.cca.2023.117650

Katalog-ID:	ELV066175844

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245	1	0	\|a Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia
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520			\|a Schizophrenia is a serious mental disease with unknown etiology that affects approximately 1 % of the population around the world. Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glutamate acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine acid (Gly), and Taurine acid (Tau) in patients with schizophrenia plasma and establish reference intervals for Chinese adult populations, and applied to patients with schizophrenia for a preliminary exploration of changes in their plasma levels of five amino acid neurotransmitters. Sample treatment involved protein precipitation followed by dansyl chloride (DNS-Cl) derivatization and total run time is 5.8 min. The method was validated according to the latest national and international guidelines, which achieved acceptable precision (0.54–14.54 %) and accuracy (97.06–103.82 %). The reference interval for Glu, Asp, Gly, Tau, and GABA were 55.51–189.06, 27.51–92.38, 204.01–574.55, 107.50–227.65, and <1 μmol/L, respectively. Increased Tau levels and decreased Asp and Glu levels were shown in patients with schizophrenia. This method was suitable for clinical routine detection of plasma 5 amino acid neurotransmitters in Chinese adult populations.
650		4	\|a Amino acid neurotransmitters
650		4	\|a Human plasma
650		4	\|a Liquid chromatography-tandem mass spectrometry
650		4	\|a Reference intervals
650		4	\|a Schizophrenia
700	1		\|a Cui, Li \|e verfasserin \|4 aut
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700	1		\|a Sun, Xiaoyu \|e verfasserin \|4 aut
700	1		\|a Zhang, Yalian \|e verfasserin \|4 aut
700	1		\|a Zhao, Furong \|e verfasserin \|4 aut
700	1		\|a Cao, Yunfeng \|e verfasserin \|0 (orcid)0000-0002-6625-5765 \|4 aut
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912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
936	b	k	\|a 35.00 \|j Chemie: Allgemeines \|q VZ
936	b	k	\|a 44.46 \|j Klinische Pathologie \|q VZ
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allfields	10.1016/j.cca.2023.117650 doi (DE-627)ELV066175844 (ELSEVIER)S0009-8981(23)00452-7 DE-627 ger DE-627 rda eng 540 610 VZ 35.00 bkl 44.46 bkl Wang, Juan verfasserin aut Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Schizophrenia is a serious mental disease with unknown etiology that affects approximately 1 % of the population around the world. Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glutamate acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine acid (Gly), and Taurine acid (Tau) in patients with schizophrenia plasma and establish reference intervals for Chinese adult populations, and applied to patients with schizophrenia for a preliminary exploration of changes in their plasma levels of five amino acid neurotransmitters. Sample treatment involved protein precipitation followed by dansyl chloride (DNS-Cl) derivatization and total run time is 5.8 min. The method was validated according to the latest national and international guidelines, which achieved acceptable precision (0.54–14.54 %) and accuracy (97.06–103.82 %). The reference interval for Glu, Asp, Gly, Tau, and GABA were 55.51–189.06, 27.51–92.38, 204.01–574.55, 107.50–227.65, and <1 μmol/L, respectively. Increased Tau levels and decreased Asp and Glu levels were shown in patients with schizophrenia. This method was suitable for clinical routine detection of plasma 5 amino acid neurotransmitters in Chinese adult populations. Amino acid neurotransmitters Human plasma Liquid chromatography-tandem mass spectrometry Reference intervals Schizophrenia Cui, Li verfasserin aut Pan, Guoliang verfasserin aut Li, Ying verfasserin aut Sun, Xiaoyu verfasserin aut Zhang, Yalian verfasserin aut Zhao, Furong verfasserin aut Cao, Yunfeng verfasserin (orcid)0000-0002-6625-5765 aut Enthalten in Clinica chimica acta Amsterdam [u.a.] : Elsevier Science, 1956 552 Online-Ressource (DE-627)306654423 (DE-600)1499920-1 (DE-576)094531242 1873-3492 nnns volume:552 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 Chemie: Allgemeines VZ 44.46 Klinische Pathologie VZ AR 552
spelling	10.1016/j.cca.2023.117650 doi (DE-627)ELV066175844 (ELSEVIER)S0009-8981(23)00452-7 DE-627 ger DE-627 rda eng 540 610 VZ 35.00 bkl 44.46 bkl Wang, Juan verfasserin aut Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Schizophrenia is a serious mental disease with unknown etiology that affects approximately 1 % of the population around the world. Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glutamate acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine acid (Gly), and Taurine acid (Tau) in patients with schizophrenia plasma and establish reference intervals for Chinese adult populations, and applied to patients with schizophrenia for a preliminary exploration of changes in their plasma levels of five amino acid neurotransmitters. Sample treatment involved protein precipitation followed by dansyl chloride (DNS-Cl) derivatization and total run time is 5.8 min. The method was validated according to the latest national and international guidelines, which achieved acceptable precision (0.54–14.54 %) and accuracy (97.06–103.82 %). The reference interval for Glu, Asp, Gly, Tau, and GABA were 55.51–189.06, 27.51–92.38, 204.01–574.55, 107.50–227.65, and <1 μmol/L, respectively. Increased Tau levels and decreased Asp and Glu levels were shown in patients with schizophrenia. This method was suitable for clinical routine detection of plasma 5 amino acid neurotransmitters in Chinese adult populations. Amino acid neurotransmitters Human plasma Liquid chromatography-tandem mass spectrometry Reference intervals Schizophrenia Cui, Li verfasserin aut Pan, Guoliang verfasserin aut Li, Ying verfasserin aut Sun, Xiaoyu verfasserin aut Zhang, Yalian verfasserin aut Zhao, Furong verfasserin aut Cao, Yunfeng verfasserin (orcid)0000-0002-6625-5765 aut Enthalten in Clinica chimica acta Amsterdam [u.a.] : Elsevier Science, 1956 552 Online-Ressource (DE-627)306654423 (DE-600)1499920-1 (DE-576)094531242 1873-3492 nnns volume:552 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 Chemie: Allgemeines VZ 44.46 Klinische Pathologie VZ AR 552
allfields_unstemmed	10.1016/j.cca.2023.117650 doi (DE-627)ELV066175844 (ELSEVIER)S0009-8981(23)00452-7 DE-627 ger DE-627 rda eng 540 610 VZ 35.00 bkl 44.46 bkl Wang, Juan verfasserin aut Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Schizophrenia is a serious mental disease with unknown etiology that affects approximately 1 % of the population around the world. Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glutamate acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine acid (Gly), and Taurine acid (Tau) in patients with schizophrenia plasma and establish reference intervals for Chinese adult populations, and applied to patients with schizophrenia for a preliminary exploration of changes in their plasma levels of five amino acid neurotransmitters. Sample treatment involved protein precipitation followed by dansyl chloride (DNS-Cl) derivatization and total run time is 5.8 min. The method was validated according to the latest national and international guidelines, which achieved acceptable precision (0.54–14.54 %) and accuracy (97.06–103.82 %). The reference interval for Glu, Asp, Gly, Tau, and GABA were 55.51–189.06, 27.51–92.38, 204.01–574.55, 107.50–227.65, and <1 μmol/L, respectively. Increased Tau levels and decreased Asp and Glu levels were shown in patients with schizophrenia. This method was suitable for clinical routine detection of plasma 5 amino acid neurotransmitters in Chinese adult populations. Amino acid neurotransmitters Human plasma Liquid chromatography-tandem mass spectrometry Reference intervals Schizophrenia Cui, Li verfasserin aut Pan, Guoliang verfasserin aut Li, Ying verfasserin aut Sun, Xiaoyu verfasserin aut Zhang, Yalian verfasserin aut Zhao, Furong verfasserin aut Cao, Yunfeng verfasserin (orcid)0000-0002-6625-5765 aut Enthalten in Clinica chimica acta Amsterdam [u.a.] : Elsevier Science, 1956 552 Online-Ressource (DE-627)306654423 (DE-600)1499920-1 (DE-576)094531242 1873-3492 nnns volume:552 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 Chemie: Allgemeines VZ 44.46 Klinische Pathologie VZ AR 552
allfieldsGer	10.1016/j.cca.2023.117650 doi (DE-627)ELV066175844 (ELSEVIER)S0009-8981(23)00452-7 DE-627 ger DE-627 rda eng 540 610 VZ 35.00 bkl 44.46 bkl Wang, Juan verfasserin aut Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Schizophrenia is a serious mental disease with unknown etiology that affects approximately 1 % of the population around the world. Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glutamate acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine acid (Gly), and Taurine acid (Tau) in patients with schizophrenia plasma and establish reference intervals for Chinese adult populations, and applied to patients with schizophrenia for a preliminary exploration of changes in their plasma levels of five amino acid neurotransmitters. Sample treatment involved protein precipitation followed by dansyl chloride (DNS-Cl) derivatization and total run time is 5.8 min. The method was validated according to the latest national and international guidelines, which achieved acceptable precision (0.54–14.54 %) and accuracy (97.06–103.82 %). The reference interval for Glu, Asp, Gly, Tau, and GABA were 55.51–189.06, 27.51–92.38, 204.01–574.55, 107.50–227.65, and <1 μmol/L, respectively. Increased Tau levels and decreased Asp and Glu levels were shown in patients with schizophrenia. This method was suitable for clinical routine detection of plasma 5 amino acid neurotransmitters in Chinese adult populations. Amino acid neurotransmitters Human plasma Liquid chromatography-tandem mass spectrometry Reference intervals Schizophrenia Cui, Li verfasserin aut Pan, Guoliang verfasserin aut Li, Ying verfasserin aut Sun, Xiaoyu verfasserin aut Zhang, Yalian verfasserin aut Zhao, Furong verfasserin aut Cao, Yunfeng verfasserin (orcid)0000-0002-6625-5765 aut Enthalten in Clinica chimica acta Amsterdam [u.a.] : Elsevier Science, 1956 552 Online-Ressource (DE-627)306654423 (DE-600)1499920-1 (DE-576)094531242 1873-3492 nnns volume:552 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 Chemie: Allgemeines VZ 44.46 Klinische Pathologie VZ AR 552
allfieldsSound	10.1016/j.cca.2023.117650 doi (DE-627)ELV066175844 (ELSEVIER)S0009-8981(23)00452-7 DE-627 ger DE-627 rda eng 540 610 VZ 35.00 bkl 44.46 bkl Wang, Juan verfasserin aut Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Schizophrenia is a serious mental disease with unknown etiology that affects approximately 1 % of the population around the world. Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glutamate acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine acid (Gly), and Taurine acid (Tau) in patients with schizophrenia plasma and establish reference intervals for Chinese adult populations, and applied to patients with schizophrenia for a preliminary exploration of changes in their plasma levels of five amino acid neurotransmitters. Sample treatment involved protein precipitation followed by dansyl chloride (DNS-Cl) derivatization and total run time is 5.8 min. The method was validated according to the latest national and international guidelines, which achieved acceptable precision (0.54–14.54 %) and accuracy (97.06–103.82 %). The reference interval for Glu, Asp, Gly, Tau, and GABA were 55.51–189.06, 27.51–92.38, 204.01–574.55, 107.50–227.65, and <1 μmol/L, respectively. Increased Tau levels and decreased Asp and Glu levels were shown in patients with schizophrenia. This method was suitable for clinical routine detection of plasma 5 amino acid neurotransmitters in Chinese adult populations. Amino acid neurotransmitters Human plasma Liquid chromatography-tandem mass spectrometry Reference intervals Schizophrenia Cui, Li verfasserin aut Pan, Guoliang verfasserin aut Li, Ying verfasserin aut Sun, Xiaoyu verfasserin aut Zhang, Yalian verfasserin aut Zhao, Furong verfasserin aut Cao, Yunfeng verfasserin (orcid)0000-0002-6625-5765 aut Enthalten in Clinica chimica acta Amsterdam [u.a.] : Elsevier Science, 1956 552 Online-Ressource (DE-627)306654423 (DE-600)1499920-1 (DE-576)094531242 1873-3492 nnns volume:552 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 Chemie: Allgemeines VZ 44.46 Klinische Pathologie VZ AR 552
language	English
source	Enthalten in Clinica chimica acta 552 volume:552
sourceStr	Enthalten in Clinica chimica acta 552 volume:552
format_phy_str_mv	Article
bklname	Chemie: Allgemeines Klinische Pathologie
institution	findex.gbv.de
topic_facet	Amino acid neurotransmitters Human plasma Liquid chromatography-tandem mass spectrometry Reference intervals Schizophrenia
dewey-raw	540
isfreeaccess_bool	false
container_title	Clinica chimica acta
authorswithroles_txt_mv	Wang, Juan @@aut@@ Cui, Li @@aut@@ Pan, Guoliang @@aut@@ Li, Ying @@aut@@ Sun, Xiaoyu @@aut@@ Zhang, Yalian @@aut@@ Zhao, Furong @@aut@@ Cao, Yunfeng @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	306654423
dewey-sort	3540
id	ELV066175844
language_de	englisch
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author	Wang, Juan
spellingShingle	Wang, Juan ddc 540 bkl 35.00 bkl 44.46 misc Amino acid neurotransmitters misc Human plasma misc Liquid chromatography-tandem mass spectrometry misc Reference intervals misc Schizophrenia Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia
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topic_title	540 610 VZ 35.00 bkl 44.46 bkl Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia Amino acid neurotransmitters Human plasma Liquid chromatography-tandem mass spectrometry Reference intervals Schizophrenia
topic	ddc 540 bkl 35.00 bkl 44.46 misc Amino acid neurotransmitters misc Human plasma misc Liquid chromatography-tandem mass spectrometry misc Reference intervals misc Schizophrenia
topic_unstemmed	ddc 540 bkl 35.00 bkl 44.46 misc Amino acid neurotransmitters misc Human plasma misc Liquid chromatography-tandem mass spectrometry misc Reference intervals misc Schizophrenia
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title	Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia
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title_full	Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia
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title_sort	simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: establishment of reference intervals in chinese adult population and application to patients with schizophrenia
title_auth	Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia
abstract	Schizophrenia is a serious mental disease with unknown etiology that affects approximately 1 % of the population around the world. Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glutamate acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine acid (Gly), and Taurine acid (Tau) in patients with schizophrenia plasma and establish reference intervals for Chinese adult populations, and applied to patients with schizophrenia for a preliminary exploration of changes in their plasma levels of five amino acid neurotransmitters. Sample treatment involved protein precipitation followed by dansyl chloride (DNS-Cl) derivatization and total run time is 5.8 min. The method was validated according to the latest national and international guidelines, which achieved acceptable precision (0.54–14.54 %) and accuracy (97.06–103.82 %). The reference interval for Glu, Asp, Gly, Tau, and GABA were 55.51–189.06, 27.51–92.38, 204.01–574.55, 107.50–227.65, and <1 μmol/L, respectively. Increased Tau levels and decreased Asp and Glu levels were shown in patients with schizophrenia. This method was suitable for clinical routine detection of plasma 5 amino acid neurotransmitters in Chinese adult populations.
abstractGer	Schizophrenia is a serious mental disease with unknown etiology that affects approximately 1 % of the population around the world. Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glutamate acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine acid (Gly), and Taurine acid (Tau) in patients with schizophrenia plasma and establish reference intervals for Chinese adult populations, and applied to patients with schizophrenia for a preliminary exploration of changes in their plasma levels of five amino acid neurotransmitters. Sample treatment involved protein precipitation followed by dansyl chloride (DNS-Cl) derivatization and total run time is 5.8 min. The method was validated according to the latest national and international guidelines, which achieved acceptable precision (0.54–14.54 %) and accuracy (97.06–103.82 %). The reference interval for Glu, Asp, Gly, Tau, and GABA were 55.51–189.06, 27.51–92.38, 204.01–574.55, 107.50–227.65, and <1 μmol/L, respectively. Increased Tau levels and decreased Asp and Glu levels were shown in patients with schizophrenia. This method was suitable for clinical routine detection of plasma 5 amino acid neurotransmitters in Chinese adult populations.
abstract_unstemmed	Schizophrenia is a serious mental disease with unknown etiology that affects approximately 1 % of the population around the world. Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glutamate acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine acid (Gly), and Taurine acid (Tau) in patients with schizophrenia plasma and establish reference intervals for Chinese adult populations, and applied to patients with schizophrenia for a preliminary exploration of changes in their plasma levels of five amino acid neurotransmitters. Sample treatment involved protein precipitation followed by dansyl chloride (DNS-Cl) derivatization and total run time is 5.8 min. The method was validated according to the latest national and international guidelines, which achieved acceptable precision (0.54–14.54 %) and accuracy (97.06–103.82 %). The reference interval for Glu, Asp, Gly, Tau, and GABA were 55.51–189.06, 27.51–92.38, 204.01–574.55, 107.50–227.65, and <1 μmol/L, respectively. Increased Tau levels and decreased Asp and Glu levels were shown in patients with schizophrenia. This method was suitable for clinical routine detection of plasma 5 amino acid neurotransmitters in Chinese adult populations.
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title_short	Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia
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author2	Cui, Li Pan, Guoliang Li, Ying Sun, Xiaoyu Zhang, Yalian Zhao, Furong Cao, Yunfeng
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Altered levels of amino acid neurotransmitters may underlie the physiopathology of schizophrenia (SZ). This study aimed to develop a rapid and robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glutamate acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine acid (Gly), and Taurine acid (Tau) in patients with schizophrenia plasma and establish reference intervals for Chinese adult populations, and applied to patients with schizophrenia for a preliminary exploration of changes in their plasma levels of five amino acid neurotransmitters. Sample treatment involved protein precipitation followed by dansyl chloride (DNS-Cl) derivatization and total run time is 5.8 min. The method was validated according to the latest national and international guidelines, which achieved acceptable precision (0.54–14.54 %) and accuracy (97.06–103.82 %). The reference interval for Glu, Asp, Gly, Tau, and GABA were 55.51–189.06, 27.51–92.38, 204.01–574.55, 107.50–227.65, and <1 μmol/L, respectively. Increased Tau levels and decreased Asp and Glu levels were shown in patients with schizophrenia. This method was suitable for clinical routine detection of plasma 5 amino acid neurotransmitters in Chinese adult populations.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Amino acid neurotransmitters</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human plasma</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Liquid chromatography-tandem mass spectrometry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Reference intervals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Schizophrenia</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cui, Li</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pan, Guoliang</subfield><subfield code="e">verfasserin</subfield><subfield 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Simultaneous determination of human plasma 5 amino acid neurotransmitters using liquid chromatography-tandem mass spectrometry: Establishment of reference intervals in Chinese adult population and application to patients with schizophrenia

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